Restriction map for
pFN21AA1849
(Linear) (Six-base) MAPSORT of: KIAA1849.seq Check: 9049 from: 1 to: 3219
KIAA1849.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1863 2399 3219
Size: 1863 536 820
Fragments arranged by size:
1863 820 536
AccI GT'mk_AC
Cuts at: 0 1706 3219
Size: 1706 1513
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 214 2259 3219
Size: 70 144 2045 960
Fragments arranged by size:
2045 960 144 70
AfeI AGC'GCT
Cuts at: 0 2780 3219
Size: 2780 439
AleI CACnn'nnGTG
Cuts at: 0 785 3219
Size: 785 2434
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1609 1641 3219
Size: 1609 32 1578
Fragments arranged by size:
1609 1578 32
AlwNI CAG_nnn'CTG
Cuts at: 0 215 1083 1206 1371 2025 2391 3015 3219
Size: 215 868 123 165 654 366 624 204
Fragments arranged by size:
868 654 624 366 215 204 165 123
ApaI G_GGCC'C
Cuts at: 0 697 1158 1963 2121 2230 2256 3219
Size: 697 461 805 158 109 26 963
Fragments arranged by size:
963 805 697 461 158 109 26
ApaLI G'TGCA_C
Cuts at: 0 1876 3219
Size: 1876 1343
AscI GG'CGCG_CC
Cuts at: 0 1102 3219
Size: 1102 2117
AvaI C'yCGr_G
Cuts at: 0 446 697 955 1091 1150 1157 1810 1882
Size: 446 251 258 136 59 7 653 72
Cuts at: 1882 2266 2488 2532 2573 2995 3021 3219
Size: 384 222 44 41 422 26 198
Fragments arranged by size:
653 446 422 384 258 251 222 198
136 72 59 44 41 26 7
BanI G'GyrC_C
Cuts at: 0 139 253 1196 2519 2989 3047 3219
Size: 139 114 943 1323 470 58 172
Fragments arranged by size:
1323 943 470 172 139 114 58
BanII G_rGCy'C
Cuts at: 0 164 616 664 697 707 950 1040 1158
Size: 164 452 48 33 10 243 90 118
Cuts at: 1158 1226 1457 1541 1963 2121 2176 2230 2256
Size: 68 231 84 422 158 55 54 26
Cuts at: 2256 2616 2662 3002 3219
Size: 360 46 340 217
Fragments arranged by size:
452 422 360 340 243 231 217 164
158 118 90 84 68 55 54 48
46 33 26 10
BbeI G_GCGC'C
Cuts at: 0 143 1200 2993 3051 3219
Size: 143 1057 1793 58 168
Fragments arranged by size:
1793 1057 168 143 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 2907 3219
Size: 2907 312
BbvCI CC'TCA_GC
Cuts at: 0 475 3219
Size: 475 2744
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 550 584 3219
Size: 550 34 2635
Fragments arranged by size:
2635 550 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3079 3219
Size: 3079 140
BglI GCCn_nnn'nGGC
Cuts at: 0 356 2152 2657 3098 3219
Size: 356 1796 505 441 121
Fragments arranged by size:
1796 505 441 356 121
BlpI GC'TnA_GC
Cuts at: 0 94 3219
Size: 94 3125
Bme1580I G_kGCm'C
Cuts at: 0 697 995 1158 1357 1759 1880 1963 2121
Size: 697 298 163 199 402 121 83 158
Cuts at: 2121 2230 2256 2488 2522 2625 3219
Size: 109 26 232 34 103 594
Fragments arranged by size:
697 594 402 298 232 199 163 158
121 109 103 83 34 26
BmgBI CAC'GTC
Cuts at: 0 342 492 3177 3219
Size: 342 150 2685 42
Fragments arranged by size:
2685 342 150 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 658 3219
Size: 658 2561
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 635 1476 1908 2630 3219
Size: 635 841 432 722 589
Fragments arranged by size:
841 722 635 589 432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 243 310 819 964 1234 1252 1432
Size: 114 129 67 509 145 270 18 180
Cuts at: 1432 1690 1771 2047 2081 2314 2545 2852 2874
Size: 258 81 276 34 233 231 307 22
Cuts at: 2874 3094 3219
Size: 220 125
Fragments arranged by size:
509 307 276 270 258 233 231 220
180 145 129 125 114 81 67 34
22 18
Bpu10I CC'TnA_GC
Cuts at: 0 475 658 1969 2729 3219
Size: 475 183 1311 760 490
Fragments arranged by size:
1311 760 490 475 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1360 2625 3219
Size: 1360 1265 594
Fragments arranged by size:
1360 1265 594
BsaI GGTCTCn'nnnn_
Cuts at: 0 643 3219
Size: 643 2576
BsaAI yAC'GTr
Cuts at: 0 330 2505 3219
Size: 330 2175 714
Fragments arranged by size:
2175 714 330
BsaHI Gr'CG_yC
Cuts at: 0 140 1197 2990 3048 3219
Size: 140 1057 1793 58 171
Fragments arranged by size:
1793 1057 171 140 58
BsaWI w'CCGG_w
Cuts at: 0 2829 3219
Size: 2829 390
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 103 133 1012 1042 1699 1729 3219
Size: 103 30 879 30 657 30 1490
Fragments arranged by size:
1490 879 657 103 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 511 608 715 1603 1666 1687 1828 1939
Size: 511 97 107 888 63 21 141 111
Cuts at: 1939 2872 3219
Size: 933 347
Fragments arranged by size:
933 888 511 347 141 111 107 97
63 21
BseYI C'CCAG_C
Cuts at: 0 170 364 844 1076 2120 2175 2329 2474
Size: 170 194 480 232 1044 55 154 145
Cuts at: 2474 2565 2710 2949 3057 3219
Size: 91 145 239 108 162
Fragments arranged by size:
1044 480 239 232 194 170 162 154
145 145 108 91 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 870 994 1617 2073 2170 2843 3219
Size: 201 669 124 623 456 97 673 376
Fragments arranged by size:
673 669 623 456 376 201 124 97
BsiEI CG_ry'CG
Cuts at: 0 2652 3041 3219
Size: 2652 389 178
Fragments arranged by size:
2652 389 178
BsiHKAI G_wGCw'C
Cuts at: 0 164 616 798 1541 1880 2069 2239 3219
Size: 164 452 182 743 339 189 170 980
Fragments arranged by size:
980 743 452 339 189 182 170 164
Bsp1286I G_dGCh'C
Cuts at: 0 164 616 664 697 707 798 950 995
Size: 164 452 48 33 10 91 152 45
Cuts at: 995 1040 1158 1226 1357 1457 1541 1759 1880
Size: 45 118 68 131 100 84 218 121
Cuts at: 1880 1963 2069 2121 2176 2230 2239 2256 2488
Size: 83 106 52 55 54 9 17 232
Cuts at: 2488 2522 2616 2625 2662 3002 3219
Size: 34 94 9 37 340 217
Fragments arranged by size:
452 340 232 218 217 164 152 131
121 118 106 100 94 91 84 83
68 55 54 52 48 45 45 37
34 33 17 10 9 9
BspEI T'CCGG_A
Cuts at: 0 2829 3219
Size: 2829 390
BspHI T'CATG_A
Cuts at: 0 1256 3219
Size: 1256 1963
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1031 1055 1863 2108 2399 3219
Size: 1031 24 808 245 291 820
Fragments arranged by size:
1031 820 808 291 245 24
BsrBI CCG'CTC
Cuts at: 0 294 906 3219
Size: 294 612 2313
Fragments arranged by size:
2313 612 294
BsrFI r'CCGG_y
Cuts at: 0 250 319 355 807 896 1130 2044 2189
Size: 250 69 36 452 89 234 914 145
Cuts at: 2189 3219
Size: 1030
Fragments arranged by size:
1030 914 452 250 234 145 89 69
36
BsrGI T'GTAC_A
Cuts at: 0 1727 3219
Size: 1727 1492
BssHII G'CGCG_C
Cuts at: 0 1102 3219
Size: 1102 2117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1371 2193 2851 3219
Size: 1371 822 658 368
Fragments arranged by size:
1371 822 658 368
BstXI CCAn_nnnn'nTGG
Cuts at: 0 516 1464 2367 3219
Size: 516 948 903 852
Fragments arranged by size:
948 903 852 516
BstYI r'GATC_y
Cuts at: 0 3194 3219
Size: 3194 25
BtgI C'CryG_G
Cuts at: 0 926 1001 1014 1478 1658 2222 2652 3219
Size: 926 75 13 464 180 564 430 567
Fragments arranged by size:
926 567 564 464 430 180 75 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 161 374 3219
Size: 161 213 2845
Fragments arranged by size:
2845 213 161
BtsI GCAGTG_nn'
Cuts at: 0 882 1705 1930 2469 3219
Size: 882 823 225 539 750
Fragments arranged by size:
882 823 750 539 225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 611 646 3219
Size: 611 35 2573
Fragments arranged by size:
2573 611 35
DraIII CAC_nnn'GTG
Cuts at: 0 817 2505 3219
Size: 817 1688 714
Fragments arranged by size:
1688 817 714
EaeI y'GGCC_r
Cuts at: 0 245 321 918 1334 1466 1643 2105 2219
Size: 245 76 597 416 132 177 462 114
Cuts at: 2219 2649 2749 3038 3219
Size: 430 100 289 181
Fragments arranged by size:
597 462 430 416 289 245 181 177
132 114 100 76
EagI C'GGCC_G
Cuts at: 0 2649 3038 3219
Size: 2649 389 181
Fragments arranged by size:
2649 389 181
EarI CTCTTCn'nnn_
Cuts at: 0 475 1918 2603 3219
Size: 475 1443 685 616
Fragments arranged by size:
1443 685 616 475
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 883 2392 3219
Size: 883 1509 827
Fragments arranged by size:
1509 883 827
EcoICRI GAG'CTC
Cuts at: 0 162 614 1539 3219
Size: 162 452 925 1680
Fragments arranged by size:
1680 925 452 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 114 214 243 310 819 964 1234
Size: 70 44 100 29 67 509 145 270
Cuts at: 1234 1252 1432 1690 1771 2047 2081 2259 2314
Size: 18 180 258 81 276 34 178 55
Cuts at: 2314 2545 2852 2874 3094 3219
Size: 231 307 22 220 125
Fragments arranged by size:
509 307 276 270 258 231 220 180
178 145 125 100 81 70 67 55
44 34 29 22 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 760 2854 3219
Size: 760 2094 365
Fragments arranged by size:
2094 760 365
EcoO109I rG'GnC_Cy
Cuts at: 0 111 693 694 1020 1062 1154 1959 1960
Size: 111 582 1 326 42 92 805 1
Cuts at: 1960 2117 2227 2252 2468 2529 2556 2628 2739
Size: 157 110 25 216 61 27 72 111
Cuts at: 2739 2938 3219
Size: 199 281
Fragments arranged by size:
805 582 326 281 216 199 157 111
111 110 92 72 61 42 27 25
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 438 3219
Size: 438 2781
FseI GG_CCGG'CC
Cuts at: 0 323 3219
Size: 323 2896
HaeII r_GCGC'y
Cuts at: 0 143 266 289 1200 1779 2045 2782 2993
Size: 143 123 23 911 579 266 737 211
Cuts at: 2993 3051 3219
Size: 58 168
Fragments arranged by size:
911 737 579 266 211 168 143 123
58 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 1012 1044 1774 1806 3219
Size: 103 32 877 32 730 32 1413
Fragments arranged by size:
1413 877 730 103 32 32 32
HincII GTy'rAC
Cuts at: 0 975 3219
Size: 975 2244
KasI G'GCGC_C
Cuts at: 0 139 1196 2989 3047 3219
Size: 139 1057 1793 58 172
Fragments arranged by size:
1793 1057 172 139 58
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 3197 3219
Size: 467 2730 22
Fragments arranged by size:
2730 467 22
MscI TGG'CCA
Cuts at: 0 247 1468 2107 2751 3219
Size: 247 1221 639 644 468
Fragments arranged by size:
1221 644 639 468 247
MslI CAynn'nnrTG
Cuts at: 0 785 1663 2131 3219
Size: 785 878 468 1088
Fragments arranged by size:
1088 878 785 468
MspA1I CmG'CkG
Cuts at: 0 66 174 372 564 682 826 917 928
Size: 66 108 198 192 118 144 91 11
Cuts at: 928 1206 1472 1596 1869 2184 2211 2810 3037
Size: 278 266 124 273 315 27 599 227
Cuts at: 3037 3219
Size: 182
Fragments arranged by size:
599 315 278 273 266 227 198 192
182 144 124 118 108 91 66 27
11
NaeI GCC'GGC
Cuts at: 0 321 357 898 1132 2046 3219
Size: 321 36 541 234 914 1173
Fragments arranged by size:
1173 914 541 321 234 36
NarI GG'CG_CC
Cuts at: 0 140 1197 2990 3048 3219
Size: 140 1057 1793 58 171
Fragments arranged by size:
1793 1057 171 140 58
NcoI C'CATG_G
Cuts at: 0 1478 1658 2222 3219
Size: 1478 180 564 997
Fragments arranged by size:
1478 997 564 180
NgoMIV G'CCGG_C
Cuts at: 0 319 355 896 1130 2044 3219
Size: 319 36 541 234 914 1175
Fragments arranged by size:
1175 914 541 319 234 36
NruI TCG'CGA
Cuts at: 0 3166 3219
Size: 3166 53
NspI r_CATG'y
Cuts at: 0 81 1578 3219
Size: 81 1497 1641
Fragments arranged by size:
1641 1497 81
PasI CC'CwG_GG
Cuts at: 0 597 690 1032 2114 2552 2618 3219
Size: 597 93 342 1082 438 66 601
Fragments arranged by size:
1082 601 597 438 342 93 66
PfoI T'CCnGG_A
Cuts at: 0 85 428 460 908 941 1211 1448 1748
Size: 85 343 32 448 33 270 237 300
Cuts at: 1748 1924 2024 3071 3219
Size: 176 100 1047 148
Fragments arranged by size:
1047 448 343 300 270 237 176 148
100 85 33 32
PmlI CAC'GTG
Cuts at: 0 2505 3219
Size: 2505 714
PpuMI rG'GwC_Cy
Cuts at: 0 111 1020 1062 2556 2739 3219
Size: 111 909 42 1494 183 480
Fragments arranged by size:
1494 909 480 183 111 42
PspOMI G'GGCC_C
Cuts at: 0 693 1154 1959 2117 2226 2252 3219
Size: 693 461 805 158 109 26 967
Fragments arranged by size:
967 805 693 461 158 109 26
PstI C_TGCA'G
Cuts at: 0 563 764 1250 1874 2018 2847 3219
Size: 563 201 486 624 144 829 372
Fragments arranged by size:
829 624 563 486 372 201 144
PvuII CAG'CTG
Cuts at: 0 66 174 1206 1472 1596 1869 2184 2211
Size: 66 108 1032 266 124 273 315 27
Cuts at: 2211 3219
Size: 1008
Fragments arranged by size:
1032 1008 315 273 266 124 108 66
27
SacI G_AGCT'C
Cuts at: 0 164 616 1541 3219
Size: 164 452 925 1678
Fragments arranged by size:
1678 925 452 164
SacII CC_GC'GG
Cuts at: 0 929 3219
Size: 929 2290
SanDI GG'GwC_CC
Cuts at: 0 2556 2739 3219
Size: 2556 183 480
Fragments arranged by size:
2556 480 183
SfcI C'TryA_G
Cuts at: 0 559 760 768 1246 1870 2014 2843 3219
Size: 559 201 8 478 624 144 829 376
Fragments arranged by size:
829 624 559 478 376 201 144 8
SfoI GGC'GCC
Cuts at: 0 141 1198 2991 3049 3219
Size: 141 1057 1793 58 170
Fragments arranged by size:
1793 1057 170 141 58
SmaI CCC'GGG
Cuts at: 0 957 1093 1159 2575 2997 3023 3219
Size: 957 136 66 1416 422 26 196
Fragments arranged by size:
1416 957 422 196 136 66 26
SmlI C'TyrA_G
Cuts at: 0 1375 2604 3219
Size: 1375 1229 615
Fragments arranged by size:
1375 1229 615
SphI G_CATG'C
Cuts at: 0 81 3219
Size: 81 3138
SrfI GCCC'GGGC
Cuts at: 0 1093 3219
Size: 1093 2126
StuI AGG'CCT
Cuts at: 0 2002 2272 2457 3219
Size: 2002 270 185 762
Fragments arranged by size:
2002 762 270 185
StyI C'CwwG_G
Cuts at: 0 509 1097 1478 1658 2222 3102 3219
Size: 509 588 381 180 564 880 117
Fragments arranged by size:
880 588 564 509 381 180 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 1354 2127 2331 3219
Size: 184 1170 773 204 888
Fragments arranged by size:
1170 888 773 204 184
TatI w'GTAC_w
Cuts at: 0 1544 1727 3219
Size: 1544 183 1492
Fragments arranged by size:
1544 1492 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2306 2338 3219
Size: 2306 32 881
Fragments arranged by size:
2306 881 32
Tth111I GACn'n_nGTC
Cuts at: 0 211 3219
Size: 211 3008
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 781 1476 1485 1665 3219
Size: 781 695 9 180 1554
Fragments arranged by size:
1554 781 695 180 9
XmaI C'CCGG_G
Cuts at: 0 955 1091 1157 2573 2995 3021 3219
Size: 955 136 66 1416 422 26 198
Fragments arranged by size:
1416 955 422 198 136 66 26
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AloI AlwNI ApaI
ApaLI AscI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstXI BstYI
BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SfcI
SfoI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI
ApoI AseI AsiSI AvrII BaeI BamHI BclI BfrBI
BglII BmtI BsaBI BsiWI BsmI BsmBI BsrDI BssSI
BstBI BstEII BstZ17I Bsu36I ClaI DraI DrdI EcoRI
EcoRV FspI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NsiI PacI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SspI SwaI XbaI XhoI XmnI ZraI