Restriction map for
pFN21AA1777
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# NO CUT ENZYMES ( 104 enzymes) #
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AccIII AflII AflIII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
AspEI AvrII BbeI BbrPI BbvCI
BciVI BfrI BlnI BlpI Bpu1102I
BsaI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV FseI HgaI HincII HindII
HindIII HpaI KasI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TthHB8I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1222 GACGTC GACGT^C
Acc65I 412 GGTACC G^GTACC
AccI 730 GTCTAC GT^[AC][GT]AC
AclI 806 AACGTT AA^CGTT
AcyI 1222 GACGTC G[GA]^CG[CT]C
Alw44I 978 GTGCAC G^TGCAC
ApaI 367 GGGCCC GGGCC^C
ApaLI 978 GTGCAC G^TGCAC
Asp700I 103 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 412 GGTACC G^GTACC
AspI 1392 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 440 TGCGCA TGC^GCA
BalI 1629 TGGCCA TGG^CCA
BamHI 968 GGATCC G^GATCC
BcgI 646 GCACGGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 632 TGATCA T^GATCA
BglI 1150 GCCGTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1323 AGATCT A^GATCT
BsaAI 733 TACGTG [CT]AC^GT[GA]
BsaBI 2719 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1222 GACGTC G[GA]^CG[CT]C
BsmBI 276 CGTCTC CGTCTC(1/5)
BsrFI 2330 ACCGGC [GA]^CCGG[CT]
Cfr10I 2330 ACCGGC [GA]^CCGG[CT]
DraI 1887 TTTAAA TTT^AAA
DraIII 1685 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 1546 ATGCAT ATGCA^T
FbaI 632 TGATCA T^GATCA
FspI 440 TGCGCA TGC^GCA
HaeII 1643 GGCGCT [GA]GCGC^[CT]
Hin1I 1222 GACGTC G[GA]^CG[CT]C
KpnI 412 GGTACC GGTAC^C
MamI 2719 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1629 TGGCCA TGG^CCA
MscI 1629 TGGCCA TGG^CCA
NsiI 1546 ATGCAT ATGCA^T
NspI 2078 GCATGT [GA]CATG^[CT]
PflFI 1392 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 387 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 806 AACGTT AA^CGTT
PvuII 2386 CAGCTG CAG^CTG
SapI 574 GAAGAGC GCTCTTC(1/4)
SmlI 2106 CTTGAG C^T[CT][GA]AG
StuI 431 AGGCCT AGG^CCT
Tth111I 1392 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 387 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1203 CCAGAGTGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 103 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 319 CGCG CG^CG
2314 CGCG
BbsI 750 GTCTTC GAAGAC(2/6)
2231 GTCTTC
BpuAI 750 GTCTTC GAAGAC(2/6)
2231 GTCTTC
BsmAI 286 GAGAC GTCTC(1/5)
277 GTCTC
BsmI 1110 GAATGC GAATGC(1/-1)
2405 GCATTC
BspHI 1083 TCATGA T^CATGA
1388 TCATGA
BsrDI 1122 CATTGC GCAATG(2/0)
776 GCAATG
BstAPI 2334 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2396 GCAAAATCTGC
BstUI 319 CGCG CG^CG
2314 CGCG
EcoRI 1305 GAATTC G^AATTC
1707 GAATTC
FauI 1845 CCCGC CCCGC(4/6)
2312 CCCGC
MvnI 319 CGCG CG^CG
2314 CGCG
NcoI 363 CCATGG C^CATGG
1518 CCATGG
PpuMI 1367 AGGACCC [GA]G^G[AT]CC[CT]
2005 GGGACCT
PstI 942 CTGCAG CTGCA^G
1363 CTGCAG
RcaI 1083 TCATGA T^CATGA
1388 TCATGA
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII