Restriction map for
pFN21AA1777
(Linear) (Six-base) MAPSORT of: KIAA1777.seq Check: 2157 from: 1 to: 2844
KIAA1777.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 682 2844
Size: 682 2162
AatII G_ACGT'C
Cuts at: 0 1226 2844
Size: 1226 1618
AccI GT'mk_AC
Cuts at: 0 731 2844
Size: 731 2113
Acc65I G'GTAC_C
Cuts at: 0 412 2844
Size: 412 2432
AclI AA'CG_TT
Cuts at: 0 807 2844
Size: 807 2037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 1808 2077 2218 2844
Size: 302 1506 269 141 626
Fragments arranged by size:
1506 626 302 269 141
AleI CACnn'nnGTG
Cuts at: 0 204 724 2844
Size: 204 520 2120
Fragments arranged by size:
2120 520 204
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 222 2844
Size: 222 2622
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 476 508 1736 1768 2290 2322 2420 2452
Size: 476 32 1228 32 522 32 98 32
Cuts at: 2452 2844
Size: 392
Fragments arranged by size:
1228 522 476 392 98 32 32 32
32
AlwNI CAG_nnn'CTG
Cuts at: 0 91 133 2187 2340 2844
Size: 91 42 2054 153 504
Fragments arranged by size:
2054 504 153 91 42
ApaI G_GGCC'C
Cuts at: 0 371 2844
Size: 371 2473
ApaLI G'TGCA_C
Cuts at: 0 978 2844
Size: 978 1866
ApoI r'AATT_y
Cuts at: 0 1009 1305 1707 2540 2643 2844
Size: 1009 296 402 833 103 201
Fragments arranged by size:
1009 833 402 296 201 103
AvaI C'yCGr_G
Cuts at: 0 370 993 1134 2844
Size: 370 623 141 1710
Fragments arranged by size:
1710 623 370 141
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 396 429 2844
Size: 396 33 2415
Fragments arranged by size:
2415 396 33
BamHI G'GATC_C
Cuts at: 0 968 2844
Size: 968 1876
BanI G'GyrC_C
Cuts at: 0 383 412 1690 2844
Size: 383 29 1278 1154
Fragments arranged by size:
1278 1154 383 29
BanII G_rGCy'C
Cuts at: 0 304 371 581 1388 1447 2844
Size: 304 67 210 807 59 1397
Fragments arranged by size:
1397 807 304 210 67 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 743 2224 2844
Size: 743 1481 620
Fragments arranged by size:
1481 743 620
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 635 669 2844
Size: 635 34 2175
Fragments arranged by size:
2175 635 34
BclI T'GATC_A
Cuts at: 0 632 2844
Size: 632 2212
BfrBI ATG'CAT
Cuts at: 0 1548 2844
Size: 1548 1296
BglI GCCn_nnn'nGGC
Cuts at: 0 1156 2844
Size: 1156 1688
BglII A'GATC_T
Cuts at: 0 1323 2844
Size: 1323 1521
Bme1580I G_kGCm'C
Cuts at: 0 371 982 2748 2844
Size: 371 611 1766 96
Fragments arranged by size:
1766 611 371 96
BmgBI CAC'GTC
Cuts at: 0 276 2844
Size: 276 2568
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 294 2844
Size: 294 2550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1738 1790 2042 2289 2380 2844
Size: 1738 52 252 247 91 464
Fragments arranged by size:
1738 464 252 247 91 52
Bpu10I CC'TnA_GC
Cuts at: 0 303 2844
Size: 303 2541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2127 2844
Size: 2127 717
BsaAI yAC'GTr
Cuts at: 0 735 2844
Size: 735 2109
BsaBI GATnn'nnATC
Cuts at: 0 2723 2844
Size: 2723 121
BsaHI Gr'CG_yC
Cuts at: 0 1223 2844
Size: 1223 1621
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 1191 1221 1736 1766 2420 2450
Size: 55 30 1106 30 515 30 654 30
Cuts at: 2450 2844
Size: 394
Fragments arranged by size:
1106 654 515 394 55 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 717 824 1717 2844
Size: 172 545 107 893 1127
Fragments arranged by size:
1127 893 545 172 107
BseYI C'CCAG_C
Cuts at: 0 40 828 914 1747 2384 2798 2844
Size: 40 788 86 833 637 414 46
Fragments arranged by size:
833 788 637 414 86 46 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 804 870 892 1029 1874 2326 2343
Size: 502 302 66 22 137 845 452 17
Cuts at: 2343 2464 2498 2844
Size: 121 34 346
Fragments arranged by size:
845 502 452 346 302 137 121 66
34 22 17
BsiHKAI G_wGCw'C
Cuts at: 0 275 304 982 1388 1447 1778 1991 2844
Size: 275 29 678 406 59 331 213 853
Fragments arranged by size:
853 678 406 331 275 213 59 29
BsmI GAATG_Cn'
Cuts at: 0 1116 2405 2844
Size: 1116 1289 439
Fragments arranged by size:
1289 1116 439
BsmBI CGTCTCn'nnnn_
Cuts at: 0 282 2844
Size: 282 2562
Bsp1286I G_dGCh'C
Cuts at: 0 275 304 371 581 982 1388 1447 1778
Size: 275 29 67 210 401 406 59 331
Cuts at: 1778 1991 2748 2844
Size: 213 757 96
Fragments arranged by size:
757 406 401 331 275 213 210 96
67 59 29
BspHI T'CATG_A
Cuts at: 0 1083 1388 2844
Size: 1083 305 1456
Fragments arranged by size:
1456 1083 305
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 682 826 892 2844
Size: 682 144 66 1952
Fragments arranged by size:
1952 682 144 66
BsrDI GCAATG_nn'
Cuts at: 0 783 1121 2844
Size: 783 338 1723
Fragments arranged by size:
1723 783 338
BsrFI r'CCGG_y
Cuts at: 0 2330 2844
Size: 2330 514
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2340 2402 2844
Size: 2340 62 442
Fragments arranged by size:
2340 442 62
BstXI CCAn_nnnn'nTGG
Cuts at: 0 693 726 1762 2267 2844
Size: 693 33 1036 505 577
Fragments arranged by size:
1036 693 577 505 33
BstYI r'GATC_y
Cuts at: 0 968 1323 2432 2844
Size: 968 355 1109 412
Fragments arranged by size:
1109 968 412 355
BtgI C'CryG_G
Cuts at: 0 363 1488 1518 2844
Size: 363 1125 30 1326
Fragments arranged by size:
1326 1125 363 30
BtsI GCAGTG_nn'
Cuts at: 0 139 519 2285 2844
Size: 139 380 1766 559
Fragments arranged by size:
1766 559 380 139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1812 1847 2844
Size: 1812 35 997
Fragments arranged by size:
1812 997 35
DraI TTT'AAA
Cuts at: 0 1889 2844
Size: 1889 955
DraIII CAC_nnn'GTG
Cuts at: 0 1690 2844
Size: 1690 1154
DrdI GACnn_nn'nnGTC
Cuts at: 0 765 1358 1816 2844
Size: 765 593 458 1028
Fragments arranged by size:
1028 765 593 458
EaeI y'GGCC_r
Cuts at: 0 716 1157 1629 2844
Size: 716 441 472 1215
Fragments arranged by size:
1215 716 472 441
EarI CTCTTCn'nnn_
Cuts at: 0 278 569 1085 1239 1888 2747 2844
Size: 278 291 516 154 649 859 97
Fragments arranged by size:
859 649 516 291 278 154 97
EcoICRI GAG'CTC
Cuts at: 0 302 1386 1445 2844
Size: 302 1084 59 1399
Fragments arranged by size:
1399 1084 302 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 1738 1790 1808 2042 2077 2218 2289
Size: 302 1436 52 18 234 35 141 71
Cuts at: 2289 2380 2844
Size: 91 464
Fragments arranged by size:
1436 464 302 234 141 91 71 52
35 18
EcoO109I rG'GnC_Cy
Cuts at: 0 848 1368 2006 2844
Size: 848 520 638 838
Fragments arranged by size:
848 838 638 520
EcoRI G'AATT_C
Cuts at: 0 1305 1707 2844
Size: 1305 402 1137
Fragments arranged by size:
1305 1137 402
FspI TGC'GCA
Cuts at: 0 442 2844
Size: 442 2402
FspAI rTGC'GCAy
Cuts at: 0 442 2844
Size: 442 2402
HaeII r_GCGC'y
Cuts at: 0 1647 2844
Size: 1647 1197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 129 161 286 318 850 882
Size: 55 32 42 32 125 32 532 32
Cuts at: 882 2015 2047 2462 2494 2716 2748 2844
Size: 1133 32 415 32 222 32 96
Fragments arranged by size:
1133 532 415 222 125 96 55 42
32 32 32 32 32 32 32
KpnI G_GTAC'C
Cuts at: 0 416 2844
Size: 416 2428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 1400 2844
Size: 41 1359 1444
Fragments arranged by size:
1444 1359 41
MscI TGG'CCA
Cuts at: 0 1631 2844
Size: 1631 1213
MslI CAynn'nnrTG
Cuts at: 0 204 676 724 1429 2174 2714 2720 2844
Size: 204 472 48 705 745 540 6 124
Fragments arranged by size:
745 705 540 472 204 124 48 6
MspA1I CmG'CkG
Cuts at: 0 1349 2388 2562 2844
Size: 1349 1039 174 282
Fragments arranged by size:
1349 1039 282 174
NcoI C'CATG_G
Cuts at: 0 363 1518 2844
Size: 363 1155 1326
Fragments arranged by size:
1326 1155 363
NsiI A_TGCA'T
Cuts at: 0 1550 2844
Size: 1550 1294
NspI r_CATG'y
Cuts at: 0 2082 2844
Size: 2082 762
PasI CC'CwG_GG
Cuts at: 0 1461 2844
Size: 1461 1383
PflMI CCAn_nnn'nTGG
Cuts at: 0 393 2844
Size: 393 2451
PfoI T'CCnGG_A
Cuts at: 0 754 811 971 1504 1991 2186 2844
Size: 754 57 160 533 487 195 658
Fragments arranged by size:
754 658 533 487 195 160 57
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1736 1768 2420 2452 2844
Size: 1736 32 652 32 392
Fragments arranged by size:
1736 652 392 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1368 2006 2844
Size: 1368 638 838
Fragments arranged by size:
1368 838 638
PspOMI G'GGCC_C
Cuts at: 0 367 2844
Size: 367 2477
PstI C_TGCA'G
Cuts at: 0 946 1367 2844
Size: 946 421 1477
Fragments arranged by size:
1477 946 421
PvuII CAG'CTG
Cuts at: 0 2388 2844
Size: 2388 456
SacI G_AGCT'C
Cuts at: 0 304 1388 1447 2844
Size: 304 1084 59 1397
Fragments arranged by size:
1397 1084 304 59
SapI GCTCTTCn'nnn_
Cuts at: 0 569 2844
Size: 569 2275
SfcI C'TryA_G
Cuts at: 0 942 1363 2823 2844
Size: 942 421 1460 21
Fragments arranged by size:
1460 942 421 21
SmlI C'TyrA_G
Cuts at: 0 2106 2844
Size: 2106 738
StuI AGG'CCT
Cuts at: 0 433 2844
Size: 433 2411
StyI C'CwwG_G
Cuts at: 0 363 1518 1737 2844
Size: 363 1155 219 1107
Fragments arranged by size:
1155 1107 363 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1132 2398 2844
Size: 1132 1266 446
Fragments arranged by size:
1266 1132 446
TatI w'GTAC_w
Cuts at: 0 513 1130 1540 2844
Size: 513 617 410 1304
Fragments arranged by size:
1304 617 513 410
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 854 886 1191 1223 1605 1637
Size: 367 32 455 32 305 32 382 32
Cuts at: 1637 1785 1817 2844
Size: 148 32 1027
Fragments arranged by size:
1027 455 382 367 305 148 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1395 2844
Size: 1395 1449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1210 2844
Size: 1210 1634
XmnI GAAnn'nnTTC
Cuts at: 0 107 2844
Size: 107 2737
ZraI GAC'GTC
Cuts at: 0 1224 2844
Size: 1224 1620
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI
BanI BanII BbsI BcgI BclI BfrBI BglI BglII
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstXI BstYI
BtgI BtsI CspCI DraI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I
KpnI MmeI MscI MslI MspA1I NcoI NsiI NspI
PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SapI SfcI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AflIII AgeI AhdI AscI AseI AsiSI
AvrII BbeI BbvCI BciVI BlpI BmrI BmtI BsaI
BsaWI BsiEI BsiWI BspEI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI EagI EciI
EcoNI EcoRV FalI FseI HincII HindIII HpaI KasI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PciI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XhoI XmaI