################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI AviII AvrII BbeI BbrPI BciVI BfrI BlnI BsaAI BsaBI BsiEI BsiWI BspDI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoT22I FseI FspI HindIII KasI KspI MamI MfeI MluI MunI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SexAI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccII 4315 CGCG CG^CG AccIII 1163 TCCGGA T^CCGGA Aor51HI 122 AGCGCT AGC^GCT BamHI 1743 GGATCC G^GATCC BcgI 3359 GCAACCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BseAI 1163 TCCGGA T^CCGGA BsmBI 3336 GAGACG CGTCTC(1/5) BsmI 2256 GCATTC GAATGC(1/-1) BspEI 1163 TCCGGA T^CCGGA BspHI 4817 TCATGA T^CATGA BsrDI 3897 CATTGC GCAATG(2/0) BsrFI 104 GCCGGC [GA]^CCGG[CT] BstUI 4315 CGCG CG^CG Cfr10I 104 GCCGGC [GA]^CCGG[CT] DraIII 1135 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3490 GACAGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 122 AGCGCT AGC^GCT EcoRV 4123 GATATC GAT^ATC HpaI 2461 GTTAAC GTT^AAC MroI 1163 TCCGGA T^CCGGA MslI 3840 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 4315 CGCG CG^CG NaeI 104 GCCGGC GCC^GGC NdeI 3290 CATATG CA^TATG NgoMIV 104 GCCGGC G^CCGGC RcaI 4817 TCATGA T^CATGA ScaI 3800 AGTACT AGT^ACT SfiI 1356 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 2532 GCATGC GCATG^C XbaI 888 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 415 GTCTAC GT^[AC][GT]AC 739 GTCTAC AocI 1207 CCTGAGG CC^T[ACGT]AGG 2430 CCTGAGG Asp700I 4654 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4821 GAAACTCTTC BbsI 1623 GAAGAC GAAGAC(2/6) 3193 GAAGAC BclI 1113 TGATCA T^GATCA 3906 TGATCA BglII 251 AGATCT A^GATCT 4656 AGATCT BpuAI 1623 GAAGAC GAAGAC(2/6) 3193 GAAGAC BsaI 373 GAGACC GGTCTC(1/5) 2745 GGTCTC BsgI 2440 CTGCAC GTGCAG(16/14) 1141 GTGCAG BsrBI 4266 CCGCTC CCGCTC(-3/-3) 1885 GAGCGG BstAPI 616 GCAGTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 778 GCAGCCAATGC Bsu36I 1207 CCTGAGG CC^T[ACGT]AGG 2430 CCTGAGG DraI 2359 TTTAAA TTT^AAA 2823 TTTAAA Eco81I 1207 CCTGAGG CC^T[ACGT]AGG 2430 CCTGAGG FbaI 1113 TGATCA T^GATCA 3906 TGATCA NspI 401 ACATGC [GA]CATG^[CT] 2532 GCATGC SapI 703 GCTCTTC GCTCTTC(1/4) 1308 GCTCTTC SbfI 1518 CCTGCAGG CCTGCA^GG 3153 CCTGCAGG Sse8387I 1518 CCTGCAGG CCTGCA^GG 3153 CCTGCAGG XmnI 4654 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4821 GAAACTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### Acc65I AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI Asp718I AspHI AspI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI