(Circular) (Six-base) MAPSORT of: pFN21AE4286.seq Check: 7894 from: 1 to: 9598 pFN21AE4286 9598 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7408 278 Size: 53 83 186 936 5872 2468 Fragments arranged by size: 5872 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2421 2745 6941 1937 Size: 484 324 4196 4594 Fragments arranged by size: 4594 4196 484 324 Acc65I G'GTAC_C Cuts at: 2280 3003 3696 6919 2280 Size: 723 693 3223 4959 Fragments arranged by size: 4959 3223 723 693 AclI AA'CG_TT Cuts at: 1563 7725 8098 1563 Size: 6162 373 3063 Fragments arranged by size: 6162 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2168 2554 2952 4649 5477 6664 7661 9045 Size: 536 386 398 1697 828 1187 997 1384 Cuts at: 9045 9313 1632 Size: 268 1917 Fragments arranged by size: 1917 1697 1384 1187 997 828 536 398 386 268 AfeI AGC'GCT Cuts at: 2129 2129 Size: 9598 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9401 AflIII A'CryG_T Cuts at: 1203 8497 9474 1203 Size: 7294 977 1327 Fragments arranged by size: 7294 1327 977 AgeI A'CCGG_T Cuts at: 8429 8429 Size: 9598 AhdI GACnn_n'nnGTC Cuts at: 8327 8327 Size: 9598 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9598 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2106 4269 1184 Size: 922 2163 6513 Fragments arranged by size: 6513 2163 922 AlwNI CAG_nnn'CTG Cuts at: 2104 3049 4675 5228 6074 6484 6631 7341 8913 Size: 945 1626 553 846 410 147 710 1572 Cuts at: 8913 2104 Size: 2789 Fragments arranged by size: 2789 1626 1572 945 846 710 553 410 147 ApaI G_GGCC'C Cuts at: 2027 3364 4408 2027 Size: 1337 1044 7217 Fragments arranged by size: 7217 1337 1044 ApaLI G'TGCA_C Cuts at: 7655 8811 7655 Size: 1156 8442 ApoI r'AATT_y Cuts at: 1493 2501 5421 5477 6907 7133 7285 9394 1493 Size: 1008 2920 56 1430 226 152 2109 1697 Fragments arranged by size: 2920 2109 1697 1430 1008 226 152 56 AseI AT'TA_AT Cuts at: 160 1032 8152 160 Size: 872 7120 1606 Fragments arranged by size: 7120 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9598 AvaI C'yCGr_G Cuts at: 1870 1943 2412 3372 4122 5330 6923 1870 Size: 73 469 960 750 1208 1593 4545 Fragments arranged by size: 4545 1593 1208 960 750 469 73 BamHI G'GATC_C Cuts at: 3748 6928 7295 3748 Size: 3180 367 6051 Fragments arranged by size: 6051 3180 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2280 3003 3696 5770 6919 Size: 325 217 627 493 723 693 2074 1149 Cuts at: 6919 8374 618 Size: 1455 1842 Fragments arranged by size: 2074 1842 1455 1149 723 693 627 493 325 217 BanII G_rGCy'C Cuts at: 1391 2027 2604 2893 2930 3315 3364 3964 4408 Size: 636 577 289 37 385 49 600 444 Cuts at: 4408 5605 5937 6917 1391 Size: 1197 332 980 4072 Fragments arranged by size: 4072 1197 980 636 600 577 444 385 332 289 49 37 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3635 5205 928 Size: 971 1736 1570 5321 Fragments arranged by size: 5321 1736 1570 971 BbvCI CC'TCA_GC Cuts at: 2058 2511 3426 4614 2058 Size: 453 915 1188 7042 Fragments arranged by size: 7042 1188 915 453 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5353 5387 7789 7823 1903 Size: 34 3416 34 2402 34 3678 Fragments arranged by size: 3678 3416 2402 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7490 8700 7490 Size: 1210 8388 BclI T'GATC_A Cuts at: 1548 3118 5911 1548 Size: 1570 2793 5235 Fragments arranged by size: 5235 2793 1570 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2888 3368 4230 Size: 107 122 71 631 605 1216 480 862 Cuts at: 4230 5633 8209 136 Size: 1403 2576 1525 Fragments arranged by size: 2576 1525 1403 1216 862 631 605 480 122 107 71 BglII A'GATC_T Cuts at: 2256 6661 9593 2256 Size: 4405 2932 2261 Fragments arranged by size: 4405 2932 2261 BlpI GC'TnA_GC Cuts at: 4257 4838 5210 5643 6624 7015 4257 Size: 581 372 433 981 391 6840 Fragments arranged by size: 6840 981 581 433 391 372 Bme1580I G_kGCm'C Cuts at: 1790 2027 3212 3364 4408 5158 6006 7659 8815 Size: 237 1185 152 1044 750 848 1653 1156 Cuts at: 8815 1790 Size: 2573 Fragments arranged by size: 2573 1653 1185 1156 1044 848 750 237 152 BmgBI CAC'GTC Cuts at: 1318 5086 1318 Size: 3768 5830 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2697 7329 8287 Size: 416 53 83 399 24 1280 4632 958 Cuts at: 8287 442 Size: 1753 Fragments arranged by size: 4632 1753 1280 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9598 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3352 3849 3352 Size: 497 9101 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2198 2540 2735 3029 3213 3263 3284 Size: 896 63 342 195 294 184 50 21 Cuts at: 3284 3386 3412 3774 4199 4367 4619 4697 4877 Size: 102 26 362 425 168 252 78 180 Cuts at: 4877 4981 5009 5030 5741 5906 5960 6785 8258 Size: 104 28 21 711 165 54 825 1473 Cuts at: 8258 1239 Size: 2579 Fragments arranged by size: 2579 1473 896 825 711 425 362 342 294 252 195 184 180 168 165 104 102 78 63 54 50 28 26 21 21 Bpu10I CC'TnA_GC Cuts at: 2058 2511 3426 3509 4614 6014 6485 2058 Size: 453 915 83 1105 1400 471 5171 Fragments arranged by size: 5171 1400 1105 915 471 453 83 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2927 3791 5341 5720 6143 7723 8588 8886 2927 Size: 864 1550 379 423 1580 865 298 3639 Fragments arranged by size: 3639 1580 1550 865 864 423 379 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2372 4756 8261 882 Size: 617 873 2384 3505 2219 Fragments arranged by size: 3505 2384 2219 873 617 BsaAI yAC'GTr Cuts at: 493 1204 7389 493 Size: 711 6185 2702 Fragments arranged by size: 6185 2702 711 BsaBI GATnn'nnATC Cuts at: 9592 9592 Size: 9598 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2531 4441 5487 5568 Size: 53 83 186 936 998 1910 1046 81 Cuts at: 5568 6226 7405 7787 8422 275 Size: 658 1179 382 635 1451 Fragments arranged by size: 1910 1451 1179 1046 998 936 658 635 382 186 83 81 53 BsaWI w'CCGG_w Cuts at: 3168 4728 6250 8031 8429 8703 8850 9261 3168 Size: 1560 1522 1781 398 274 147 411 3505 Fragments arranged by size: 3505 1781 1560 1522 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3191 3221 4121 4151 4979 Size: 30 165 30 1788 30 900 30 828 Cuts at: 4979 5009 5352 5382 6155 6185 1178 Size: 30 343 30 773 30 4591 Fragments arranged by size: 4591 1788 900 828 773 343 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 2075 2132 2168 2195 2998 3323 3695 Size: 840 49 57 36 27 803 325 372 Cuts at: 3695 3788 4142 4542 5360 5798 6524 6863 1186 Size: 93 354 400 818 438 726 339 3921 Fragments arranged by size: 3921 840 818 803 726 438 400 372 354 339 325 93 57 49 36 27 BseYI C'CCAG_C Cuts at: 2031 2215 2458 3049 3219 3447 4288 5548 5616 Size: 184 243 591 170 228 841 1260 68 Cuts at: 5616 8801 9297 2031 Size: 3185 496 2332 Fragments arranged by size: 3185 2332 1260 841 591 496 243 228 184 170 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3167 4430 1163 Size: 573 1431 1263 6331 Fragments arranged by size: 6331 1431 1263 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7048 7809 8837 1540 Size: 275 186 5047 761 1028 2301 Fragments arranged by size: 5047 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2604 2710 3279 3315 5211 5892 5937 6110 6491 Size: 106 569 36 1896 681 45 173 381 Cuts at: 6491 7659 7744 8815 2604 Size: 1168 85 1071 3387 Fragments arranged by size: 3387 1896 1168 1071 681 569 381 173 106 85 45 36 BsmI GAATG_Cn' Cuts at: 4261 7109 7202 4261 Size: 2848 93 6657 Fragments arranged by size: 6657 2848 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5335 5335 Size: 9598 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2604 2710 2893 2930 3212 3279 Size: 399 237 577 106 183 37 282 67 Cuts at: 3279 3315 3364 3964 4408 5158 5211 5605 5892 Size: 36 49 600 444 750 53 394 287 Cuts at: 5892 5937 6006 6110 6491 6917 7659 7744 8815 Size: 45 69 104 381 426 742 85 1071 Cuts at: 8815 1391 Size: 2174 Fragments arranged by size: 2174 1071 750 742 600 577 444 426 399 394 381 287 282 237 183 106 104 85 69 67 53 49 45 37 36 BspEI T'CCGG_A Cuts at: 3168 9261 3168 Size: 6093 3505 BspHI T'CATG_A Cuts at: 6822 7487 9217 6822 Size: 665 1730 7203 Fragments arranged by size: 7203 1730 665 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3531 4896 6342 6953 844 Size: 2687 1365 1446 611 3489 Fragments arranged by size: 3489 2687 1446 1365 611 BsrBI CCG'CTC Cuts at: 1394 1823 3892 6273 7485 1394 Size: 429 2069 2381 1212 3507 Fragments arranged by size: 3507 2381 2069 1212 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5901 8093 8267 65 Size: 1183 4653 2192 174 1396 Fragments arranged by size: 4653 2192 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2109 8242 8429 1700 Size: 110 299 6133 187 2869 Fragments arranged by size: 6133 2869 299 187 110 BsrGI T'GTAC_A Cuts at: 96 4983 5841 6236 96 Size: 4887 858 395 3458 Fragments arranged by size: 4887 3458 858 395 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9598 BssSI C'ACGA_G Cuts at: 7658 8670 7658 Size: 1012 8586 BstAPI GCAn_nnn'nTGC Cuts at: 794 2627 2789 794 Size: 1833 162 7603 Fragments arranged by size: 7603 1833 162 BstBI TT'CG_AA Cuts at: 8402 8402 Size: 9598 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9598 BstXI CCAn_nnnn'nTGG Cuts at: 2291 3421 4912 4960 5836 6748 2291 Size: 1130 1491 48 876 912 5141 Fragments arranged by size: 5141 1491 1130 912 876 48 BstYI r'GATC_y Cuts at: 1972 2256 3748 3777 4074 4131 4302 4392 4482 Size: 284 1492 29 297 57 171 90 90 Cuts at: 4482 4656 5944 6165 6661 6787 6928 7295 7680 Size: 174 1288 221 496 126 141 367 385 Cuts at: 7680 7697 9149 9235 9247 9593 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1492 1452 1288 496 385 367 346 297 284 221 174 171 141 126 90 90 86 57 29 17 12 Bsu36I CC'TnA_GG Cuts at: 3213 4436 3213 Size: 1223 8375 BtgI C'CryG_G Cuts at: 513 1065 2004 2238 2382 2884 3975 4228 5586 Size: 552 939 234 144 502 1091 253 1358 Cuts at: 5586 5835 5850 513 Size: 249 15 4261 Fragments arranged by size: 4261 1358 1091 939 552 502 253 249 234 144 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5338 5974 487 Size: 683 56 4112 636 4111 Fragments arranged by size: 4112 4111 683 636 56 BtsI GCAGTG_nn' Cuts at: 2661 3279 3918 4038 4155 4756 4974 5802 6257 Size: 618 639 120 117 601 218 828 455 Cuts at: 6257 6615 7114 7908 7928 2661 Size: 358 499 794 20 4331 Fragments arranged by size: 4331 828 794 639 618 601 499 455 358 218 120 117 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9598 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9563 DraI TTT'AAA Cuts at: 4366 4830 6902 4366 Size: 464 2072 7062 Fragments arranged by size: 7062 2072 464 DraIII CAC_nnn'GTG Cuts at: 3145 3145 Size: 9598 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5501 8605 809 Size: 507 4185 3104 1802 Fragments arranged by size: 4185 3104 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2541 2592 Size: 54 1425 50 275 18 378 333 51 Cuts at: 2592 3301 4914 5445 5583 5826 6123 6402 7045 Size: 709 1613 531 138 243 297 279 643 Cuts at: 7045 7934 9563 8 Size: 889 1629 43 Fragments arranged by size: 1629 1613 1425 889 709 643 531 378 333 297 279 275 243 138 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7045 1537 Size: 275 5233 4090 Fragments arranged by size: 5233 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2450 2456 2715 3320 3558 4635 4886 6612 Size: 1097 6 259 605 238 1077 251 1726 Cuts at: 6612 6836 7528 1353 Size: 224 692 3423 Fragments arranged by size: 3423 1726 1097 1077 692 605 259 251 238 224 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3770 6457 8185 8559 8705 387 Size: 1064 138 2181 2687 1728 374 146 1280 Fragments arranged by size: 2687 2181 1728 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2602 3313 5935 2602 Size: 711 2622 6265 Fragments arranged by size: 6265 2622 711 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2168 2198 2540 2554 2735 2952 Size: 393 503 33 30 342 14 181 217 Cuts at: 2952 3029 3213 3263 3284 3386 3412 3774 4199 Size: 77 184 50 21 102 26 362 425 Cuts at: 4199 4367 4619 4649 4697 4877 4981 5009 5030 Size: 168 252 30 48 180 104 28 21 Cuts at: 5030 5477 5741 5906 5960 6664 6785 7661 8258 Size: 447 264 165 54 704 121 876 597 Cuts at: 8258 9045 9313 1239 Size: 787 268 1524 Fragments arranged by size: 1524 876 787 704 597 503 447 425 393 362 342 268 264 252 217 184 181 180 168 165 121 104 102 77 54 50 48 33 30 30 28 26 21 21 14 EcoNI CCTnn'n_nnAGG Cuts at: 2150 2843 3414 3425 6635 2150 Size: 693 571 11 3210 5113 Fragments arranged by size: 5113 3210 693 571 11 EcoO109I rG'GnC_Cy Cuts at: 2947 3361 3577 4278 4684 5704 6481 2947 Size: 414 216 701 406 1020 777 6064 Fragments arranged by size: 6064 1020 777 701 414 406 216 EcoRI G'AATT_C Cuts at: 6907 7285 6907 Size: 378 9220 EcoRV GAT'ATC Cuts at: 6130 6130 Size: 9598 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4942 6517 6661 4942 Size: 1575 144 7879 Fragments arranged by size: 7879 1575 144 FspI TGC'GCA Cuts at: 8104 8104 Size: 9598 HaeII r_GCGC'y Cuts at: 1678 2131 5623 6369 8745 1678 Size: 453 3492 746 2376 2531 Fragments arranged by size: 3492 2531 2376 746 453 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2467 2499 3733 3765 4121 Size: 32 135 32 895 32 1234 32 356 Cuts at: 4121 4153 4294 4326 5337 5369 5792 5824 6155 Size: 32 141 32 1011 32 423 32 331 Cuts at: 6155 6187 8239 8271 8313 8345 1373 Size: 32 2052 32 42 32 2626 Fragments arranged by size: 2626 2052 1234 1011 895 423 356 331 141 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2878 4468 5866 6856 6942 7188 Size: 988 281 940 1590 1398 990 86 246 Cuts at: 7188 669 Size: 3079 Fragments arranged by size: 3079 1590 1398 990 988 940 281 246 86 HindIII A'AGCT_T Cuts at: 748 748 Size: 9598 HpaI GTT'AAC Cuts at: 4468 7188 4468 Size: 2720 6878 KpnI G_GTAC'C Cuts at: 2284 3007 3700 6923 2284 Size: 723 693 3223 4959 Fragments arranged by size: 4959 3223 723 693 MfeI C'AATT_G Cuts at: 7197 7197 Size: 9598 MluI A'CGCG_T Cuts at: 9474 9474 Size: 9598 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6108 8712 8896 1305 Size: 4803 2604 184 2007 Fragments arranged by size: 4803 2604 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 2543 2594 3303 4916 Size: 54 1425 343 378 333 51 709 1613 Cuts at: 4916 5447 5585 5828 6404 10 Size: 531 138 243 576 3204 Fragments arranged by size: 3204 1613 1425 709 576 531 378 343 333 243 138 54 51 MslI CAynn'nnrTG Cuts at: 518 1166 5849 7556 7915 8074 518 Size: 648 4683 1707 359 159 2042 Fragments arranged by size: 4683 2042 1707 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2021 2057 2275 2510 2560 2633 3016 3151 Size: 415 36 218 235 50 73 383 135 Cuts at: 3151 3223 3262 3341 3659 4142 4333 4675 4792 Size: 72 39 79 318 483 191 342 117 Cuts at: 4792 5029 5552 5594 6118 6214 6316 7014 7691 Size: 237 523 42 524 96 102 698 677 Cuts at: 7691 8839 9084 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 698 677 524 523 483 415 383 342 318 245 237 235 218 191 135 117 102 96 79 73 72 50 42 39 36 NaeI GCC'GGC Cuts at: 2111 2111 Size: 9598 NcoI C'CATG_G Cuts at: 513 1065 2004 2884 3975 5586 5835 513 Size: 552 939 880 1091 1611 249 4276 Fragments arranged by size: 4276 1611 1091 939 880 552 249 NdeI CA'TA_TG Cuts at: 387 5296 387 Size: 4909 4689 NgoMIV G'CCGG_C Cuts at: 2109 2109 Size: 9598 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9598 NotI GC'GGCC_GC Cuts at: 7045 7045 Size: 9598 NspI r_CATG'y Cuts at: 1132 1229 2410 4541 6956 8501 1132 Size: 97 1181 2131 2415 1545 2229 Fragments arranged by size: 2415 2229 2131 1545 1181 97 PasI CC'CwG_GG Cuts at: 1348 5629 6352 1348 Size: 4281 723 4594 Fragments arranged by size: 4594 4281 723 PciI A'CATG_T Cuts at: 8497 8497 Size: 9598 PflMI CCAn_nnn'nTGG Cuts at: 1108 2130 3790 5086 5629 1108 Size: 1022 1660 1296 543 5077 Fragments arranged by size: 5077 1660 1296 1022 543 PfoI T'CCnGG_A Cuts at: 3751 4635 5046 5184 5937 6630 3751 Size: 884 411 138 753 693 6719 Fragments arranged by size: 6719 884 753 693 411 138 PmeI GTTT'AAAC Cuts at: 6902 6902 Size: 9598 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3915 3947 7667 7699 9180 9212 3915 Size: 32 3720 32 1481 32 4301 Fragments arranged by size: 4301 3720 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3577 4278 4684 5704 3577 Size: 701 406 1020 7471 Fragments arranged by size: 7471 1020 701 406 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9598 PsiI TTA'TAA Cuts at: 7168 7168 Size: 9598 PspOMI G'GGCC_C Cuts at: 2023 3360 4404 2023 Size: 1337 1044 7217 Fragments arranged by size: 7217 1337 1044 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5513 5545 5513 Size: 32 9566 PstI C_TGCA'G Cuts at: 830 2623 3103 3528 3658 4062 4743 5163 5998 Size: 1793 480 425 130 404 681 420 835 Cuts at: 5998 6468 6798 6950 830 Size: 470 330 152 3478 Fragments arranged by size: 3478 1793 835 681 480 470 425 420 404 330 152 130 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9598 PvuII CAG'CTG Cuts at: 2057 2275 2510 2560 2633 3016 3223 3262 3341 Size: 218 235 50 73 383 207 39 79 Cuts at: 3341 3659 4142 4675 4792 5029 5552 5594 6118 Size: 318 483 533 117 237 523 42 524 Cuts at: 6118 6214 2057 Size: 96 5441 Fragments arranged by size: 5441 533 524 523 483 383 318 237 235 218 207 117 96 79 73 50 42 39 SacI G_AGCT'C Cuts at: 2604 3315 5937 2604 Size: 711 2622 6265 Fragments arranged by size: 6265 2622 711 SalI G'TCGA_C Cuts at: 1936 6940 1936 Size: 5004 4594 SapI GCTCTTCn'nnn_ Cuts at: 2715 3320 2715 Size: 605 8993 SbfI CC_TGCA'GG Cuts at: 3528 5163 6950 3528 Size: 1635 1787 6176 Fragments arranged by size: 6176 1787 1635 ScaI AGT'ACT Cuts at: 5807 7846 9558 5807 Size: 2039 1712 5847 Fragments arranged by size: 5847 2039 1712 SfcI C'TryA_G Cuts at: 826 1045 2619 3099 3524 3654 4058 4522 4739 Size: 219 1574 480 425 130 404 464 217 Cuts at: 4739 5159 5651 5994 6464 6526 6794 6946 8081 Size: 420 492 343 470 62 268 152 1135 Cuts at: 8081 8762 8953 826 Size: 681 191 1471 Fragments arranged by size: 1574 1471 1135 681 492 480 470 464 425 420 404 343 268 219 217 191 152 130 62 SfiI GGCCn_nnn'nGGCC Cuts at: 3368 3368 Size: 9598 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9598 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9598 SmaI CCC'GGG Cuts at: 1872 2414 3374 4124 6925 1872 Size: 542 960 750 2801 4545 Fragments arranged by size: 4545 2801 960 750 542 SmlI C'TyrA_G Cuts at: 820 1017 1943 2942 3770 5320 5735 6158 7702 Size: 197 926 999 828 1550 415 423 1544 Cuts at: 7702 8603 8865 820 Size: 901 262 1553 Fragments arranged by size: 1553 1550 1544 999 926 901 828 423 415 262 197 SnaBI TAC'GTA Cuts at: 493 7389 493 Size: 6896 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9598 SphI G_CATG'C Cuts at: 1132 4541 6956 1132 Size: 3409 2415 3774 Fragments arranged by size: 3774 3409 2415 SspI AAT'ATT Cuts at: 5 52 7522 5 Size: 47 7470 2081 Fragments arranged by size: 7470 2081 47 StuI AGG'CCT Cuts at: 1517 2141 3420 4400 5012 5318 5675 6233 1517 Size: 624 1279 980 612 306 357 558 4882 Fragments arranged by size: 4882 1279 980 624 612 558 357 306 StyI C'CwwG_G Cuts at: 513 1065 2004 2884 3975 4149 5586 5835 6405 Size: 552 939 880 1091 174 1437 249 570 Cuts at: 6405 7037 513 Size: 632 3074 Fragments arranged by size: 3074 1437 1091 939 880 632 570 552 249 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2342 3485 6740 7625 7642 7795 7980 Size: 1255 762 1143 3255 885 17 153 185 Cuts at: 7980 8425 325 Size: 445 1498 Fragments arranged by size: 3255 1498 1255 1143 885 762 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4983 5529 5805 Size: 274 80 33 51 1443 3006 546 276 Cuts at: 5805 5841 6236 6534 7844 9556 96 Size: 36 395 298 1310 1712 138 Fragments arranged by size: 3006 1712 1443 1310 546 395 298 276 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3191 3223 9558 9590 3191 Size: 32 6335 32 3199 Fragments arranged by size: 6335 3199 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3575 3634 5228 1316 Size: 2259 59 1594 5686 Fragments arranged by size: 5686 2259 1594 59 XbaI T'CTAG_A Cuts at: 2893 6934 2893 Size: 4041 5557 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2131 2710 4672 4957 5426 6118 2131 Size: 579 1962 285 469 692 5611 Fragments arranged by size: 5611 1962 692 579 469 285 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9598 XmaI C'CCGG_G Cuts at: 1870 2412 3372 4122 6923 1870 Size: 542 960 750 2801 4545 Fragments arranged by size: 4545 2801 960 750 542 XmnI GAAnn'nnTTC Cuts at: 6663 6830 7727 6663 Size: 167 897 8534 Fragments arranged by size: 8534 897 167 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7406 276 Size: 53 83 186 936 5872 2468 Fragments arranged by size: 5872 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI RsrII SacII SanDI SexAI SfoI SrfI SwaI