Restriction map for
pFN21AA1411
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AocI Aor51HI
ApaI AscI Asp718I AspI AvaI
AvrII BalI BanII BbeI BbrPI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BsrFI BssHII BstAPI
BstBI BstEII BstPI BstUI Bsu36I
CelII Cfr10I ClaI CpoI DsaI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I FseI HaeII
HapII HgaI Hin1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NcoI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1404 GTATAC GT^[AC][GT]AC
AseI 1524 ATTAAT AT^TAAT
AsnI 1524 ATTAAT AT^TAAT
AviII 921 TGCGCA TGC^GCA
BanI 1387 GGTGCC G^G[CT][GA]CC
BbvCI 2413 CCTCAGC CCTCAGC(-5/-2)
BcgI 2627 CGACTGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2832 GGATAC GTATCC(6/5)
BclI 1932 TGATCA T^GATCA
BsaBI 1195 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4117 GGTCTC GGTCTC(1/5)
BseRI 3256 GAGGAG GAGGAG(10/8)
BspHI 186 TCATGA T^CATGA
BssSI 3476 CACGAG CACGAG(-5/-1)
Bst1107I 1404 GTATAC GTA^TAC
BstXI 1307 CCAGTAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1404 GTATAC GTA^TAC
CfoI 922 GCGC GCG^C
DraIII 728 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1697 GACAGACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1855 GATATC GAT^ATC
FauI 4298 CCCGC CCCGC(4/6)
FbaI 1932 TGATCA T^GATCA
FspI 921 TGCGCA TGC^GCA
HhaI 922 GCGC GCG^C
HinP1I 922 GCGC G^CGC
MamI 1195 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1967 CAATTG C^AATTG
MunI 1967 CAATTG C^AATTG
NdeI 2282 CATATG CA^TATG
PflMI 1394 CCAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1524 ATTAAT AT^TAAT
PstI 1152 CTGCAG CTGCA^G
RcaI 186 TCATGA T^CATGA
SapI 3262 GAAGAGC GCTCTTC(1/4)
SmlI 310 CTTGAG C^T[CT][GA]AG
SphI 147 GCATGC GCATG^C
StuI 438 AGGCCT AGG^CCT
Van91I 1394 CCAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2293 TCTAGA T^CTAGA
XcmI 2819 CCAAACCTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AciI 1130 CCGC CCGC(-3/-1)
4299 CCGC
AflIII 3160 ACATGT A^C[GA][CT]GT
4168 ACATGT
AhdI 3897 GACTGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4330 GACAAACAGTC
Alw44I 1125 GTGCAC G^TGCAC
3760 GTGCAC
AlwNI 958 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1247 CAGTTACTG
ApaLI 1125 GTGCAC G^TGCAC
3760 GTGCAC
Asp700I 790 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1771 GAAAATGTTC
AspEI 3897 GACTGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4330 GACAAACAGTC
BamHI 1650 GGATCC G^GATCC
4261 GGATCC
BbsI 558 GAAGAC GAAGAC(2/6)
1255 GAAGAC
BpuAI 558 GAAGAC GAAGAC(2/6)
1255 GAAGAC
BsmFI 954 GGGAC GGGAC(10/14)
2884 GGGAC
Bsp1407I 224 TGTACA T^GTACA
3665 TGTACA
BspLU11I 3160 ACATGT A^CATGT
4168 ACATGT
BspMI 1380 ACCTGC ACCTGC(4/8)
2208 ACCTGC
BsrGI 224 TGTACA T^GTACA
3665 TGTACA
DraII 2028 AGGACCT [GA]G^G[ACGT]CC[CT]
2385 AGGTCCT
Eam1105I 3897 GACTGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4330 GACAAACAGTC
EcoO109I 2028 AGGACCT [GA]G^G[ACGT]CC[CT]
2385 AGGTCCT
EcoT22I 506 ATGCAT ATGCA^T
4430 ATGCAT
HaeIII 439 GGCC GG^CC
4016 GGCC
MspA1I 4162 CAGCTG C[AC]G^C[GT]G
4445 CAGCTG
NheI 747 GCTAGC G^CTAGC
1717 GCTAGC
NsiI 506 ATGCAT ATGCA^T
4430 ATGCAT
PpuMI 2028 AGGACCT [GA]G^G[AT]CC[CT]
2385 AGGTCCT
PvuII 4162 CAGCTG CAG^CTG
4445 CAGCTG
ScaI 3088 AGTACT AGT^ACT
3547 AGTACT
SspBI 224 TGTACA T^GTACA
3665 TGTACA
Tsp45I 2547 GTCAC ^GT[GC]AC
3404 GTGAC
XmnI 790 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1771 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BbvI BfaI BglII
BmyI BpmI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII