(Circular) (Six-base) MAPSORT of: pFN21AA1411.seq Check: 5758 from: 1 to: 9250 pFN21AA1411 9250 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7060 278 Size: 53 83 186 936 5524 2468 Fragments arranged by size: 5524 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3410 6593 1937 Size: 1473 3183 4594 Fragments arranged by size: 4594 3183 1473 Acc65I G'GTAC_C Cuts at: 6571 6571 Size: 9250 AclI AA'CG_TT Cuts at: 1563 7377 7750 1563 Size: 5814 373 3063 Fragments arranged by size: 5814 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2031 3279 3281 3357 5219 5609 5663 5757 Size: 399 1248 2 76 1862 390 54 94 Cuts at: 5757 7313 8697 8965 1632 Size: 1556 1384 268 1917 Fragments arranged by size: 1917 1862 1556 1384 1248 399 390 268 94 76 54 2 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9053 AflIII A'CryG_T Cuts at: 1203 5165 6173 8149 9126 1203 Size: 3962 1008 1976 977 1327 Fragments arranged by size: 3962 1976 1327 1008 977 AgeI A'CCGG_T Cuts at: 8081 8081 Size: 9250 AhdI GACnn_n'nnGTC Cuts at: 5907 6340 7979 5907 Size: 433 1639 7178 Fragments arranged by size: 7178 1639 433 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9250 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9250 AlwNI CAG_nnn'CTG Cuts at: 2968 3257 6993 8565 2968 Size: 289 3736 1572 3653 Fragments arranged by size: 3736 3653 1572 289 ApaLI G'TGCA_C Cuts at: 3130 5765 7307 8463 3130 Size: 2635 1542 1156 3917 Fragments arranged by size: 3917 2635 1542 1156 ApoI r'AATT_y Cuts at: 1493 2030 2109 2121 2223 2326 3285 3835 4451 Size: 537 79 12 102 103 959 550 616 Cuts at: 4451 4564 4744 4906 5522 6515 6559 6785 6937 Size: 113 180 162 616 993 44 226 152 Cuts at: 6937 9046 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 993 959 616 616 550 537 226 180 162 152 113 103 102 79 44 12 AseI AT'TA_AT Cuts at: 160 1032 3530 7804 160 Size: 872 2498 4274 1606 Fragments arranged by size: 4274 2498 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9250 AvaI C'yCGr_G Cuts at: 1870 1943 6575 1870 Size: 73 4632 4545 Fragments arranged by size: 4632 4545 73 BamHI G'GATC_C Cuts at: 3655 6266 6580 6947 3655 Size: 2611 314 367 5958 Fragments arranged by size: 5958 2611 367 314 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3392 6571 8026 618 Size: 325 217 627 1605 3179 1455 1842 Fragments arranged by size: 3179 1842 1605 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 6569 1391 Size: 5178 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2570 3267 928 Size: 971 671 697 6911 Fragments arranged by size: 6911 971 697 671 BbvCI CC'TCA_GC Cuts at: 4419 4419 Size: 9250 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4621 4655 7441 7475 1903 Size: 34 2684 34 2786 34 3678 Fragments arranged by size: 3678 2786 2684 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4831 7142 8352 4831 Size: 2311 1210 5729 Fragments arranged by size: 5729 2311 1210 BclI T'GATC_A Cuts at: 1548 3937 1548 Size: 2389 6861 BfrBI ATG'CAT Cuts at: 2513 6437 2513 Size: 3924 5326 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7861 136 Size: 107 122 71 631 605 6189 1525 Fragments arranged by size: 6189 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3205 5791 6351 9245 3205 Size: 2586 560 2894 3210 Fragments arranged by size: 3210 2894 2586 560 BlpI GC'TnA_GC Cuts at: 6667 6667 Size: 9250 Bme1580I G_kGCm'C Cuts at: 1790 3134 5769 7311 8467 1790 Size: 1344 2635 1542 1156 2573 Fragments arranged by size: 2635 2573 1542 1344 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9250 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3981 6981 7939 Size: 416 53 83 399 24 2564 3000 958 Cuts at: 7939 442 Size: 1753 Fragments arranged by size: 3000 2564 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2756 3726 1056 Size: 1700 970 6580 Fragments arranged by size: 6580 1700 970 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2846 3382 3750 7910 1239 Size: 1607 536 368 4160 2579 Fragments arranged by size: 4160 2579 1607 536 368 Bpu10I CC'TnA_GC Cuts at: 4419 4419 Size: 9250 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 7375 8240 8538 2336 Size: 5039 865 298 3048 Fragments arranged by size: 5039 3048 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6128 7913 882 Size: 617 4629 1785 2219 Fragments arranged by size: 4629 2219 1785 617 BsaAI yAC'GTr Cuts at: 493 1204 7041 493 Size: 711 5837 2702 Fragments arranged by size: 5837 2702 711 BsaBI GATnn'nnATC Cuts at: 3204 9244 3204 Size: 6040 3210 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7057 7439 8074 275 Size: 53 83 186 936 5524 382 635 1451 Fragments arranged by size: 5524 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7683 8081 8355 8502 8913 7683 Size: 398 274 147 411 8020 Fragments arranged by size: 8020 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 9025 Fragments arranged by size: 9025 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 5276 1186 Size: 4090 5160 BseYI C'CCAG_C Cuts at: 8453 8949 8453 Size: 496 8754 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2408 5460 5808 6459 1163 Size: 573 672 3052 348 651 3954 Fragments arranged by size: 3954 3052 672 651 573 348 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6700 7461 8489 1540 Size: 275 186 4699 761 1028 2301 Fragments arranged by size: 4699 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3021 3134 5769 6109 7311 7396 8467 3021 Size: 113 2635 340 1202 85 1071 3804 Fragments arranged by size: 3804 2635 1202 1071 340 113 85 BsmI GAATG_Cn' Cuts at: 2392 3053 4595 6761 6854 2392 Size: 661 1542 2166 93 4788 Fragments arranged by size: 4788 2166 1542 661 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3021 3134 5769 6109 6569 7311 7396 Size: 399 1231 113 2635 340 460 742 85 Cuts at: 7396 8467 1391 Size: 1071 2174 Fragments arranged by size: 2635 2174 1231 1071 742 460 399 340 113 85 BspEI T'CCGG_A Cuts at: 8913 8913 Size: 9250 BspHI T'CATG_A Cuts at: 2191 7139 8869 2191 Size: 4948 1730 2572 Fragments arranged by size: 4948 2572 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3394 4222 6605 844 Size: 2550 828 2383 3489 Fragments arranged by size: 3489 2550 2383 828 BsrBI CCG'CTC Cuts at: 1394 1823 7137 1394 Size: 429 5314 3507 Fragments arranged by size: 5314 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2028 2688 3087 5191 6437 7745 7919 Size: 1183 780 660 399 2104 1246 1308 174 Cuts at: 7919 65 Size: 1396 Fragments arranged by size: 2104 1396 1308 1246 1183 780 660 399 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7894 8081 1700 Size: 110 6084 187 2869 Fragments arranged by size: 6084 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2229 5670 96 Size: 2133 3441 3676 Fragments arranged by size: 3676 3441 2133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9250 BssSI C'ACGA_G Cuts at: 5481 7310 8322 5481 Size: 1829 1012 6409 Fragments arranged by size: 6409 1829 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9250 BstBI TT'CG_AA Cuts at: 8054 8054 Size: 9250 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9250 BstXI CCAn_nnnn'nTGG Cuts at: 3319 3319 Size: 9250 BstYI r'GATC_y Cuts at: 1972 2344 3205 3655 3997 5791 6266 6351 6580 Size: 372 861 450 342 1794 475 85 229 Cuts at: 6580 6947 7332 7349 8801 8887 8899 9245 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1794 1452 861 475 450 385 372 367 346 342 229 86 85 17 12 BstZ17I GTA'TAC Cuts at: 3411 3411 Size: 9250 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 8698 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8511 Fragments arranged by size: 8511 683 56 BtsI GCAGTG_nn' Cuts at: 6109 6766 7560 7580 6109 Size: 657 794 20 7779 Fragments arranged by size: 7779 794 657 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9250 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9215 DraI TTT'AAA Cuts at: 2253 6031 6330 6554 2253 Size: 3778 299 224 4949 Fragments arranged by size: 4949 3778 299 224 DraIII CAC_nnn'GTG Cuts at: 2738 2738 Size: 9250 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3708 8257 809 Size: 507 2392 4549 1802 Fragments arranged by size: 4549 2392 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6697 7586 9215 Size: 54 1425 50 275 18 4867 889 1629 Cuts at: 9215 8 Size: 43 Fragments arranged by size: 4867 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6697 1537 Size: 275 4885 4090 Fragments arranged by size: 4885 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2235 2790 3171 4782 5262 5718 7180 1353 Size: 882 555 381 1611 480 456 1462 3423 Fragments arranged by size: 3423 1611 1462 882 555 480 456 381 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7837 8211 8357 387 Size: 1064 138 6248 374 146 1280 Fragments arranged by size: 6248 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2846 3279 3281 3357 3382 3750 Size: 393 399 815 433 2 76 25 368 Cuts at: 3750 5219 5609 5663 5757 7313 7910 8697 8965 Size: 1469 390 54 94 1556 597 787 268 Cuts at: 8965 1239 Size: 1524 Fragments arranged by size: 1556 1524 1469 815 787 597 433 399 393 390 368 268 94 76 54 25 2 EcoO109I rG'GnC_Cy Cuts at: 4034 4391 4034 Size: 357 8893 EcoRI G'AATT_C Cuts at: 2030 2121 3285 6559 6937 2030 Size: 91 1164 3274 378 4343 Fragments arranged by size: 4343 3274 1164 378 91 EcoRV GAT'ATC Cuts at: 3862 3862 Size: 9250 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2330 2443 2867 4692 5097 5399 5571 5639 6251 Size: 113 424 1825 405 302 172 68 612 Cuts at: 6251 2330 Size: 5329 Fragments arranged by size: 5329 1825 612 424 405 302 172 113 68 FspI TGC'GCA Cuts at: 2928 7756 2928 Size: 4828 4422 HaeII r_GCGC'y Cuts at: 1678 8397 1678 Size: 6719 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2605 2637 4535 4567 5893 Size: 32 135 32 1033 32 1898 32 1326 Cuts at: 5893 5925 5932 5964 6326 6358 7891 7923 7965 Size: 32 7 32 362 32 1533 32 42 Cuts at: 7965 7997 1373 Size: 32 2626 Fragments arranged by size: 2626 1898 1533 1326 1033 362 135 42 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 6594 6840 669 Size: 988 281 4656 246 3079 Fragments arranged by size: 4656 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2545 3491 4149 5968 6215 748 Size: 1797 946 658 1819 247 3783 Fragments arranged by size: 3783 1819 1797 946 658 247 HpaI GTT'AAC Cuts at: 6840 6840 Size: 9250 KpnI G_GTAC'C Cuts at: 6575 6575 Size: 9250 MfeI C'AATT_G Cuts at: 3972 6849 3972 Size: 2877 6373 MluI A'CGCG_T Cuts at: 9126 9126 Size: 9250 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 8364 8548 1305 Size: 7059 184 2007 Fragments arranged by size: 7059 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7428 Fragments arranged by size: 7428 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2454 2460 4599 5024 6311 7208 7567 Size: 648 1288 6 2139 425 1287 897 359 Cuts at: 7567 7726 518 Size: 159 2042 Fragments arranged by size: 2139 2042 1288 1287 897 648 425 359 159 6 MspA1I CmG'CkG Cuts at: 1606 6169 6452 6666 7343 8491 8736 1606 Size: 4563 283 214 677 1148 245 2120 Fragments arranged by size: 4563 2120 1148 677 283 245 214 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8698 NdeI CA'TA_TG Cuts at: 387 4288 387 Size: 3901 5349 NheI G'CTAG_C Cuts at: 1052 2752 3722 1052 Size: 1700 970 6580 Fragments arranged by size: 6580 1700 970 NotI GC'GGCC_GC Cuts at: 6697 6697 Size: 9250 NsiI A_TGCA'T Cuts at: 2515 6439 2515 Size: 3924 5326 NspI r_CATG'y Cuts at: 1132 1229 2156 5169 5437 6177 6608 8153 1132 Size: 97 927 3013 268 740 431 1545 2229 Fragments arranged by size: 3013 2229 1545 927 740 431 268 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9250 PciI A'CATG_T Cuts at: 5165 6173 8149 5165 Size: 1008 1976 6266 Fragments arranged by size: 6266 1976 1008 PflMI CCAn_nnn'nTGG Cuts at: 1108 3405 1108 Size: 2297 6953 PmeI GTTT'AAAC Cuts at: 6554 6554 Size: 9250 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7319 7351 8832 8864 7319 Size: 32 1481 32 7705 Fragments arranged by size: 7705 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4034 4391 4034 Size: 357 8893 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9250 PsiI TTA'TAA Cuts at: 2912 2978 4443 6820 2912 Size: 66 1465 2377 5342 Fragments arranged by size: 5342 2377 1465 66 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5631 5663 5631 Size: 32 9218 PstI C_TGCA'G Cuts at: 830 3161 6602 830 Size: 2331 3441 3478 Fragments arranged by size: 3478 3441 2331 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9250 PvuII CAG'CTG Cuts at: 6169 6452 6169 Size: 283 8967 SalI G'TCGA_C Cuts at: 1936 6592 1936 Size: 4656 4594 SapI GCTCTTCn'nnn_ Cuts at: 5262 5262 Size: 9250 SbfI CC_TGCA'GG Cuts at: 6602 6602 Size: 9250 ScaI AGT'ACT Cuts at: 5095 5554 7498 9210 5095 Size: 459 1944 1712 5135 Fragments arranged by size: 5135 1944 1712 459 SfcI C'TryA_G Cuts at: 826 1045 3122 3157 3348 4125 4725 4884 5043 Size: 219 2077 35 191 777 600 159 159 Cuts at: 5043 5179 6272 6598 7733 8414 8605 826 Size: 136 1093 326 1135 681 191 1471 Fragments arranged by size: 2077 1471 1135 1093 777 681 600 326 219 191 191 159 159 136 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9250 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9250 SmaI CCC'GGG Cuts at: 1872 6577 1872 Size: 4705 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2315 7354 8255 8517 820 Size: 197 926 372 5039 901 262 1553 Fragments arranged by size: 5039 1553 926 901 372 262 197 SnaBI TAC'GTA Cuts at: 493 7041 493 Size: 6548 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9250 SphI G_CATG'C Cuts at: 1132 2156 6608 1132 Size: 1024 4452 3774 Fragments arranged by size: 4452 3774 1024 SspI AAT'ATT Cuts at: 5 52 3003 5507 6035 6504 6542 7174 5 Size: 47 2951 2504 528 469 38 632 2081 Fragments arranged by size: 2951 2504 2081 632 528 469 47 38 StuI AGG'CCT Cuts at: 1517 2445 1517 Size: 928 8322 StyI C'CwwG_G Cuts at: 513 1065 2340 4037 6023 6082 6689 513 Size: 552 1275 1697 1986 59 607 3074 Fragments arranged by size: 3074 1986 1697 1275 607 552 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 7277 7294 7447 7632 8077 325 Size: 1255 5697 17 153 185 445 1498 Fragments arranged by size: 5697 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2229 3587 4472 Size: 274 80 33 51 1443 252 1358 885 Cuts at: 4472 4481 5093 5447 5552 5670 5746 7496 9208 Size: 9 612 354 105 118 76 1750 1712 Cuts at: 9208 96 Size: 138 Fragments arranged by size: 1750 1712 1443 1358 885 612 354 274 252 138 118 105 80 76 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4829 4861 9210 9242 4829 Size: 32 4349 32 4837 Fragments arranged by size: 4837 4349 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9250 XbaI T'CTAG_A Cuts at: 4298 6586 4298 Size: 2288 6962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4831 4831 Size: 9250 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9250 XmaI C'CCGG_G Cuts at: 1870 6575 1870 Size: 4705 4545 XmnI GAAnn'nnTTC Cuts at: 2799 3780 7379 2799 Size: 981 3599 4670 Fragments arranged by size: 4670 3599 981 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7058 276 Size: 53 83 186 936 5524 2468 Fragments arranged by size: 5524 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI