Restriction map for
pFN21AA1411
(Linear) (Six-base) MAPSORT of: KIAA1411.seq Check: 5658 from: 1 to: 4545
KIAA1411.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AccI GT'mk_AC
Cuts at: 0 1405 4545
Size: 1405 3140
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 1274 1276 1352 3214 3604 3658 3752
Size: 26 1248 2 76 1862 390 54 94
Cuts at: 3752 4545
Size: 793
Fragments arranged by size:
1862 1248 793 390 94 76 54 26
2
AflIII A'CryG_T
Cuts at: 0 3160 4168 4545
Size: 3160 1008 377
Fragments arranged by size:
3160 1008 377
AhdI GACnn_n'nnGTC
Cuts at: 0 3902 4335 4545
Size: 3902 433 210
Fragments arranged by size:
3902 433 210
AlwNI CAG_nnn'CTG
Cuts at: 0 963 1252 4545
Size: 963 289 3293
Fragments arranged by size:
3293 963 289
ApaLI G'TGCA_C
Cuts at: 0 1125 3760 4545
Size: 1125 2635 785
Fragments arranged by size:
2635 1125 785
ApoI r'AATT_y
Cuts at: 0 25 104 116 218 321 1280 1830 2446
Size: 25 79 12 102 103 959 550 616
Cuts at: 2446 2559 2739 2901 3517 4510 4545
Size: 113 180 162 616 993 35
Fragments arranged by size:
993 959 616 616 550 180 162 113
103 102 79 35 25 12
AseI AT'TA_AT
Cuts at: 0 1525 4545
Size: 1525 3020
BamHI G'GATC_C
Cuts at: 0 1650 4261 4545
Size: 1650 2611 284
Fragments arranged by size:
2611 1650 284
BanI G'GyrC_C
Cuts at: 0 1387 4545
Size: 1387 3158
BbsI GAAGACnn'nnnn_
Cuts at: 0 565 1262 4545
Size: 565 697 3283
Fragments arranged by size:
3283 697 565
BbvCI CC'TCA_GC
Cuts at: 0 2414 4545
Size: 2414 2131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2616 2650 4545
Size: 2616 34 1895
Fragments arranged by size:
2616 1895 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2826 4545
Size: 2826 1719
BclI T'GATC_A
Cuts at: 0 1932 4545
Size: 1932 2613
BfrBI ATG'CAT
Cuts at: 0 508 4432 4545
Size: 508 3924 113
Fragments arranged by size:
3924 508 113
BglII A'GATC_T
Cuts at: 0 1200 3786 4346 4545
Size: 1200 2586 560 199
Fragments arranged by size:
2586 1200 560 199
Bme1580I G_kGCm'C
Cuts at: 0 1129 3764 4545
Size: 1129 2635 781
Fragments arranged by size:
2635 1129 781
BmrI ACTGGGnnnn_n'
Cuts at: 0 1976 4545
Size: 1976 2569
BmtI G_CTAG'C
Cuts at: 0 751 1721 4545
Size: 751 970 2824
Fragments arranged by size:
2824 970 751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 1377 1745 4545
Size: 841 536 368 2800
Fragments arranged by size:
2800 841 536 368
Bpu10I CC'TnA_GC
Cuts at: 0 2414 4545
Size: 2414 2131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 4545
Size: 331 4214
BsaI GGTCTCn'nnnn_
Cuts at: 0 4123 4545
Size: 4123 422
BsaBI GATnn'nnATC
Cuts at: 0 1199 4545
Size: 1199 3346
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3271 4545
Size: 3271 1274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 3455 3803 4454 4545
Size: 403 3052 348 651 91
Fragments arranged by size:
3052 651 403 348 91
BsiHKAI G_wGCw'C
Cuts at: 0 1016 1129 3764 4104 4545
Size: 1016 113 2635 340 441
Fragments arranged by size:
2635 1016 441 340 113
BsmI GAATG_Cn'
Cuts at: 0 387 1048 2590 4545
Size: 387 661 1542 1955
Fragments arranged by size:
1955 1542 661 387
Bsp1286I G_dGCh'C
Cuts at: 0 1016 1129 3764 4104 4545
Size: 1016 113 2635 340 441
Fragments arranged by size:
2635 1016 441 340 113
BspHI T'CATG_A
Cuts at: 0 186 4545
Size: 186 4359
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1389 2217 4545
Size: 1389 828 2328
Fragments arranged by size:
2328 1389 828
BsrDI GCAATG_nn'
Cuts at: 0 23 683 1082 3186 4432 4545
Size: 23 660 399 2104 1246 113
Fragments arranged by size:
2104 1246 660 399 113 23
BsrGI T'GTAC_A
Cuts at: 0 224 3665 4545
Size: 224 3441 880
Fragments arranged by size:
3441 880 224
BssSI C'ACGA_G
Cuts at: 0 3476 4545
Size: 3476 1069
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1314 4545
Size: 1314 3231
BstYI r'GATC_y
Cuts at: 0 339 1200 1650 1992 3786 4261 4346 4545
Size: 339 861 450 342 1794 475 85 199
Fragments arranged by size:
1794 861 475 450 342 339 199 85
BstZ17I GTA'TAC
Cuts at: 0 1406 4545
Size: 1406 3139
BtsI GCAGTG_nn'
Cuts at: 0 4104 4545
Size: 4104 441
DraI TTT'AAA
Cuts at: 0 248 4026 4325 4545
Size: 248 3778 299 220
Fragments arranged by size:
3778 299 248 220
DraIII CAC_nnn'GTG
Cuts at: 0 733 4545
Size: 733 3812
DrdI GACnn_nn'nnGTC
Cuts at: 0 1703 4545
Size: 1703 2842
EarI CTCTTCn'nnn_
Cuts at: 0 230 785 1166 2777 3257 3713 4545
Size: 230 555 381 1611 480 456 832
Fragments arranged by size:
1611 832 555 480 456 381 230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 841 1274 1276 1352 1377 1745 3214
Size: 26 815 433 2 76 25 368 1469
Cuts at: 3214 3604 3658 3752 4545
Size: 390 54 94 793
Fragments arranged by size:
1469 815 793 433 390 368 94 76
54 26 25 2
EcoO109I rG'GnC_Cy
Cuts at: 0 2029 2386 4545
Size: 2029 357 2159
Fragments arranged by size:
2159 2029 357
EcoRI G'AATT_C
Cuts at: 0 25 116 1280 4545
Size: 25 91 1164 3265
Fragments arranged by size:
3265 1164 91 25
EcoRV GAT'ATC
Cuts at: 0 1857 4545
Size: 1857 2688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 325 438 862 2687 3092 3394 3566 3634
Size: 325 113 424 1825 405 302 172 68
Cuts at: 3634 4246 4545
Size: 612 299
Fragments arranged by size:
1825 612 424 405 325 302 299 172
113 68
FspI TGC'GCA
Cuts at: 0 923 4545
Size: 923 3622
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 600 632 2530 2562 3888 3920 3927 3959
Size: 600 32 1898 32 1326 32 7 32
Cuts at: 3959 4321 4353 4545
Size: 362 32 192
Fragments arranged by size:
1898 1326 600 362 192 32 32 32
32 32 7
HindIII A'AGCT_T
Cuts at: 0 540 1486 2144 3963 4210 4545
Size: 540 946 658 1819 247 335
Fragments arranged by size:
1819 946 658 540 335 247
MfeI C'AATT_G
Cuts at: 0 1967 4545
Size: 1967 2578
MslI CAynn'nnrTG
Cuts at: 0 449 455 2594 3019 4306 4545
Size: 449 6 2139 425 1287 239
Fragments arranged by size:
2139 1287 449 425 239 6
MspA1I CmG'CkG
Cuts at: 0 4164 4447 4545
Size: 4164 283 98
Fragments arranged by size:
4164 283 98
NdeI CA'TA_TG
Cuts at: 0 2283 4545
Size: 2283 2262
NheI G'CTAG_C
Cuts at: 0 747 1717 4545
Size: 747 970 2828
Fragments arranged by size:
2828 970 747
NsiI A_TGCA'T
Cuts at: 0 510 4434 4545
Size: 510 3924 111
Fragments arranged by size:
3924 510 111
NspI r_CATG'y
Cuts at: 0 151 3164 3432 4172 4545
Size: 151 3013 268 740 373
Fragments arranged by size:
3013 740 373 268 151
PciI A'CATG_T
Cuts at: 0 3160 4168 4545
Size: 3160 1008 377
Fragments arranged by size:
3160 1008 377
PflMI CCAn_nnn'nTGG
Cuts at: 0 1400 4545
Size: 1400 3145
PpuMI rG'GwC_Cy
Cuts at: 0 2029 2386 4545
Size: 2029 357 2159
Fragments arranged by size:
2159 2029 357
PsiI TTA'TAA
Cuts at: 0 907 973 2438 4545
Size: 907 66 1465 2107
Fragments arranged by size:
2107 1465 907 66
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3626 3658 4545
Size: 3626 32 887
Fragments arranged by size:
3626 887 32
PstI C_TGCA'G
Cuts at: 0 1156 4545
Size: 1156 3389
PvuII CAG'CTG
Cuts at: 0 4164 4447 4545
Size: 4164 283 98
Fragments arranged by size:
4164 283 98
SapI GCTCTTCn'nnn_
Cuts at: 0 3257 4545
Size: 3257 1288
ScaI AGT'ACT
Cuts at: 0 3090 3549 4545
Size: 3090 459 996
Fragments arranged by size:
3090 996 459
SfcI C'TryA_G
Cuts at: 0 1117 1152 1343 2120 2720 2879 3038 3174
Size: 1117 35 191 777 600 159 159 136
Cuts at: 3174 4267 4545
Size: 1093 278
Fragments arranged by size:
1117 1093 777 600 278 191 159 159
136 35
SmlI C'TyrA_G
Cuts at: 0 310 4545
Size: 310 4235
SphI G_CATG'C
Cuts at: 0 151 4545
Size: 151 4394
SspI AAT'ATT
Cuts at: 0 998 3502 4030 4499 4537 4545
Size: 998 2504 528 469 38 8
Fragments arranged by size:
2504 998 528 469 38 8
StuI AGG'CCT
Cuts at: 0 440 4545
Size: 440 4105
StyI C'CwwG_G
Cuts at: 0 335 2032 4018 4077 4545
Size: 335 1697 1986 59 468
Fragments arranged by size:
1986 1697 468 335 59
TatI w'GTAC_w
Cuts at: 0 224 1582 2467 2476 3088 3442 3547 3665
Size: 224 1358 885 9 612 354 105 118
Cuts at: 3665 3741 4545
Size: 76 804
Fragments arranged by size:
1358 885 804 612 354 224 118 105
76 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2824 2856 4545
Size: 2824 32 1689
Fragments arranged by size:
2824 1689 32
XbaI T'CTAG_A
Cuts at: 0 2293 4545
Size: 2293 2252
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2826 4545
Size: 2826 1719
XmnI GAAnn'nnTTC
Cuts at: 0 794 1775 4545
Size: 794 981 2770
Fragments arranged by size:
2770 981 794
Enzymes that do cut:
AccI AcuI AflIII AhdI AlwNI ApaLI ApoI AseI
BamHI BanI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaBI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrGI BssSI BstXI BstYI BstZ17I BtsI DraI
DraIII DrdI EarI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI Hin4I HindIII MfeI MslI MspA1I NdeI NheI
NsiI NspI PciI PflMI PpuMI PsiI PsrI PstI
PvuII SapI ScaI SfcI SmlI SphI SspI StuI
StyI TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI AscI AsiSI AvaI AvrII BaeI
BanII BbeI BglI BlpI BmgBI BplI BsaAI BsaHI
BsaWI BsaXI BseYI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BstAPI BstBI BstEII Bsu36I BtgI BtgZI
ClaI CspCI EaeI EagI EciI EcoICRI EcoNI FseI
FspAI HaeII HincII HpaI KasI KpnI MluI MmeI
MscI NaeI NarI NcoI NgoMIV NotI NruI PacI
PasI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SwaI TaqII Tth111I XhoI XmaI ZraI