(Circular) (Six-base) MAPSORT of: pFN21AA1275.seq Check: 6609 from: 1 to: 8236 pFN21AA1275 8236 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3878 4513 3878 Size: 635 7601 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4409 6046 278 Size: 53 83 186 936 2873 1637 2468 Fragments arranged by size: 2873 2468 1637 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2544 3699 5042 5579 1937 Size: 607 1155 1343 537 4594 Fragments arranged by size: 4594 1343 1155 607 537 Acc65I G'GTAC_C Cuts at: 5557 5557 Size: 8236 AclI AA'CG_TT Cuts at: 1563 4881 6363 6736 1563 Size: 3318 1482 373 3063 Fragments arranged by size: 3318 3063 1482 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 3941 4821 5232 6299 7683 7951 1632 Size: 695 1614 880 411 1067 1384 268 1917 Fragments arranged by size: 1917 1614 1384 1067 880 695 411 268 AfeI AGC'GCT Cuts at: 4346 4346 Size: 8236 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8039 AflIII A'CryG_T Cuts at: 1203 7135 8112 1203 Size: 5932 977 1327 Fragments arranged by size: 5932 1327 977 AgeI A'CCGG_T Cuts at: 5401 7067 5401 Size: 1666 6570 AhdI GACnn_n'nnGTC Cuts at: 6965 6965 Size: 8236 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8236 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2409 4083 1184 Size: 1225 1674 5337 Fragments arranged by size: 5337 1674 1225 AlwNI CAG_nnn'CTG Cuts at: 2165 2827 4732 5979 7551 2165 Size: 662 1905 1247 1572 2850 Fragments arranged by size: 2850 1905 1572 1247 662 ApaI G_GGCC'C Cuts at: 4668 4668 Size: 8236 ApaLI G'TGCA_C Cuts at: 3349 6293 7449 3349 Size: 2944 1156 4136 Fragments arranged by size: 4136 2944 1156 ApoI r'AATT_y Cuts at: 1493 3598 4001 4675 4805 5202 5545 5771 5923 Size: 2105 403 674 130 397 343 226 152 Cuts at: 5923 8032 1493 Size: 2109 1697 Fragments arranged by size: 2109 2105 1697 674 403 397 343 226 152 130 AseI AT'TA_AT Cuts at: 160 1032 4205 6790 160 Size: 872 3173 2585 1606 Fragments arranged by size: 3173 2585 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8236 AvaI C'yCGr_G Cuts at: 1870 1943 5421 5561 1870 Size: 73 3478 140 4545 Fragments arranged by size: 4545 3478 140 73 BamHI G'GATC_C Cuts at: 5566 5933 5566 Size: 367 7869 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4689 5557 7012 618 Size: 325 217 627 2902 868 1455 1842 Fragments arranged by size: 2902 1842 1455 868 627 325 217 BanII G_rGCy'C Cuts at: 1391 3267 3867 4360 4593 4668 5555 1391 Size: 1876 600 493 233 75 887 4072 Fragments arranged by size: 4072 1876 887 600 493 233 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2244 2315 2883 3974 4979 928 Size: 971 345 71 568 1091 1005 4185 Fragments arranged by size: 4185 1091 1005 971 568 345 71 BbvCI CC'TCA_GC Cuts at: 2828 2828 Size: 8236 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6427 6461 1903 Size: 34 4490 34 3678 Fragments arranged by size: 4490 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4581 6128 7338 4581 Size: 1547 1210 5479 Fragments arranged by size: 5479 1547 1210 BclI T'GATC_A Cuts at: 1548 3985 1548 Size: 2437 5799 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6847 136 Size: 107 122 71 631 605 5175 1525 Fragments arranged by size: 5175 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2009 4550 8231 2009 Size: 2541 3681 2014 Fragments arranged by size: 3681 2541 2014 BlpI GC'TnA_GC Cuts at: 5653 5653 Size: 8236 Bme1580I G_kGCm'C Cuts at: 1790 3353 4668 4692 6297 7453 1790 Size: 1563 1315 24 1605 1156 2573 Fragments arranged by size: 2573 1605 1563 1315 1156 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8236 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3205 4362 4937 Size: 416 53 83 399 24 1788 1157 575 Cuts at: 4937 5425 5967 6925 442 Size: 488 542 958 1753 Fragments arranged by size: 1788 1753 1157 958 575 542 488 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8236 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2952 2952 Size: 8236 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2211 2243 2357 2636 3392 4220 4434 4560 Size: 972 32 114 279 756 828 214 126 Cuts at: 4560 4962 5133 5515 6896 1239 Size: 402 171 382 1381 2579 Fragments arranged by size: 2579 1381 972 828 756 402 382 279 214 171 126 114 32 Bpu10I CC'TnA_GC Cuts at: 2828 2828 Size: 8236 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2486 2879 2922 2987 6361 7226 7524 2486 Size: 393 43 65 3374 865 298 3198 Fragments arranged by size: 3374 3198 865 393 298 65 43 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5226 6899 882 Size: 617 3727 1673 2219 Fragments arranged by size: 3727 2219 1673 617 BsaAI yAC'GTr Cuts at: 493 1204 6027 493 Size: 711 4823 2702 Fragments arranged by size: 4823 2702 711 BsaBI GATnn'nnATC Cuts at: 8230 8230 Size: 8236 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4406 6043 6425 7060 Size: 53 83 186 936 2873 1637 382 635 Cuts at: 7060 275 Size: 1451 Fragments arranged by size: 2873 1637 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5401 6669 7067 7341 7488 7899 5401 Size: 1268 398 274 147 411 5738 Fragments arranged by size: 5738 1268 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2415 2445 4628 4658 4689 Size: 30 165 30 1012 30 2183 30 31 Cuts at: 4689 4719 1178 Size: 30 4695 Fragments arranged by size: 4695 2183 1012 165 31 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3047 3203 4064 5344 1186 Size: 1861 156 861 1280 4078 Fragments arranged by size: 4078 1861 1280 861 156 BseYI C'CCAG_C Cuts at: 3354 5460 7439 7935 3354 Size: 2106 1979 496 3655 Fragments arranged by size: 3655 2106 1979 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 1163 Size: 573 633 7030 Fragments arranged by size: 7030 633 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5686 6447 7475 1540 Size: 275 186 3685 761 1028 2301 Fragments arranged by size: 3685 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3267 3353 3867 4360 4593 5387 6297 6382 7453 Size: 86 514 493 233 794 910 85 1071 Cuts at: 7453 3267 Size: 4050 Fragments arranged by size: 4050 1071 910 794 514 493 233 86 85 BsmI GAATG_Cn' Cuts at: 5481 5747 5840 5481 Size: 266 93 7877 Fragments arranged by size: 7877 266 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3935 3935 Size: 8236 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3267 3353 3867 4360 4593 4668 4692 Size: 399 1477 86 514 493 233 75 24 Cuts at: 4692 5387 5555 6297 6382 7453 1391 Size: 695 168 742 85 1071 2174 Fragments arranged by size: 2174 1477 1071 742 695 514 493 399 233 168 86 85 75 24 BspEI T'CCGG_A Cuts at: 7899 7899 Size: 8236 BspHI T'CATG_A Cuts at: 6125 7855 6125 Size: 1730 6506 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2619 3408 3878 4513 5591 844 Size: 1775 789 470 635 1078 3489 Fragments arranged by size: 3489 1775 1078 789 635 470 BsrBI CCG'CTC Cuts at: 1394 1823 2626 6123 1394 Size: 429 803 3497 3507 Fragments arranged by size: 3507 3497 803 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2086 6731 6905 65 Size: 1183 838 4645 174 1396 Fragments arranged by size: 4645 1396 1183 838 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2490 5318 5401 6880 7067 1700 Size: 110 680 2828 83 1479 187 2869 Fragments arranged by size: 2869 2828 1479 680 187 110 83 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8236 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8236 BssSI C'ACGA_G Cuts at: 4757 6296 7308 4757 Size: 1539 1012 5685 Fragments arranged by size: 5685 1539 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2512 794 Size: 1718 6518 BstBI TT'CG_AA Cuts at: 3154 7040 3154 Size: 3886 4350 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8236 BstXI CCAn_nnnn'nTGG Cuts at: 2614 4330 2614 Size: 1716 6520 BstYI r'GATC_y Cuts at: 1972 2009 2812 4550 5566 5933 6318 6335 7787 Size: 37 803 1738 1016 367 385 17 1452 Cuts at: 7787 7873 7885 8231 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1738 1452 1016 803 385 367 346 86 37 17 12 BstZ17I GTA'TAC Cuts at: 2545 3700 2545 Size: 1155 7081 Bsu36I CC'TnA_GG Cuts at: 2158 2328 4837 2158 Size: 170 2509 5557 Fragments arranged by size: 5557 2509 170 BtgI C'CryG_G Cuts at: 513 1065 4542 4616 5097 513 Size: 552 3477 74 481 3652 Fragments arranged by size: 3652 3477 552 481 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7497 Fragments arranged by size: 7497 683 56 BtsI GCAGTG_nn' Cuts at: 3784 4767 5752 6546 6566 3784 Size: 983 985 794 20 5454 Fragments arranged by size: 5454 985 983 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8236 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8201 DraI TTT'AAA Cuts at: 3366 3669 5540 3366 Size: 303 1871 6062 Fragments arranged by size: 6062 1871 303 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7243 809 Size: 507 5927 1802 Fragments arranged by size: 5927 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2517 5683 6572 Size: 54 1425 50 275 18 687 3166 889 Cuts at: 6572 8201 8 Size: 1629 43 Fragments arranged by size: 3166 1629 1425 889 687 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5683 1537 Size: 275 3871 4090 Fragments arranged by size: 4090 3871 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 2835 2943 3036 3287 3564 3791 4984 Size: 761 721 108 93 251 277 227 1193 Cuts at: 4984 6166 1353 Size: 1182 3423 Fragments arranged by size: 3423 1193 1182 761 721 277 251 227 108 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6823 7197 7343 387 Size: 1064 138 5234 374 146 1280 Fragments arranged by size: 5234 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3265 3865 4358 4591 3265 Size: 600 493 233 6910 Fragments arranged by size: 6910 600 493 233 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2211 2243 2327 2357 2636 3392 3941 Size: 393 579 32 84 30 279 756 549 Cuts at: 3941 4220 4434 4560 4821 4962 5133 5232 5515 Size: 279 214 126 261 141 171 99 283 Cuts at: 5515 6299 6896 7683 7951 1239 Size: 784 597 787 268 1524 Fragments arranged by size: 1524 787 784 756 597 579 549 393 283 279 279 268 261 214 171 141 126 99 84 32 30 EcoNI CCTnn'n_nnAGG Cuts at: 4836 4836 Size: 8236 EcoO109I rG'GnC_Cy Cuts at: 2380 4664 4665 2380 Size: 2284 1 5951 Fragments arranged by size: 5951 2284 1 EcoRI G'AATT_C Cuts at: 5545 5923 5545 Size: 378 7858 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3512 4045 4241 3512 Size: 533 196 7507 Fragments arranged by size: 7507 533 196 FspI TGC'GCA Cuts at: 6742 6742 Size: 8236 HaeII r_GCGC'y Cuts at: 1678 4348 7383 1678 Size: 2670 3035 2531 Fragments arranged by size: 3035 2670 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2415 2447 4689 4721 5094 Size: 32 135 32 843 32 2242 32 373 Cuts at: 5094 5126 5441 5473 6877 6909 6951 6983 1373 Size: 32 315 32 1404 32 42 32 2626 Fragments arranged by size: 2626 2242 1404 843 373 315 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3784 4410 5580 5826 669 Size: 988 281 1846 626 1170 246 3079 Fragments arranged by size: 3079 1846 1170 988 626 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8236 HpaI GTT'AAC Cuts at: 5826 5826 Size: 8236 KpnI G_GTAC'C Cuts at: 5561 5561 Size: 8236 MfeI C'AATT_G Cuts at: 3938 5835 3938 Size: 1897 6339 MluI A'CGCG_T Cuts at: 8112 8112 Size: 8236 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2272 3958 4119 4311 5168 5305 7350 7534 Size: 967 1686 161 192 857 137 2045 184 Cuts at: 7534 1305 Size: 2007 Fragments arranged by size: 2045 2007 1686 967 857 192 184 161 137 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6414 Fragments arranged by size: 6414 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2391 2753 5486 6194 6553 6712 518 Size: 648 1225 362 2733 708 359 159 2042 Fragments arranged by size: 2733 2042 1225 708 648 362 359 159 MspA1I CmG'CkG Cuts at: 1606 2509 3991 4437 4466 5060 5464 5652 6329 Size: 903 1482 446 29 594 404 188 677 Cuts at: 6329 7477 7722 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1482 1148 903 677 594 446 404 245 188 29 NcoI C'CATG_G Cuts at: 513 1065 4616 5097 513 Size: 552 3551 481 3652 Fragments arranged by size: 3652 3551 552 481 NdeI CA'TA_TG Cuts at: 387 387 Size: 8236 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8236 NotI GC'GGCC_GC Cuts at: 5683 5683 Size: 8236 NspI r_CATG'y Cuts at: 1132 1229 2305 2500 5594 7139 1132 Size: 97 1076 195 3094 1545 2229 Fragments arranged by size: 3094 2229 1545 1076 195 97 PasI CC'CwG_GG Cuts at: 1348 5240 1348 Size: 3892 4344 PciI A'CATG_T Cuts at: 7135 7135 Size: 8236 PflMI CCAn_nnn'nTGG Cuts at: 1108 3940 4985 1108 Size: 2832 1045 4359 Fragments arranged by size: 4359 2832 1045 PfoI T'CCnGG_A Cuts at: 4488 4488 Size: 8236 PmeI GTTT'AAAC Cuts at: 5540 5540 Size: 8236 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6305 6337 7818 7850 6305 Size: 32 1481 32 6691 Fragments arranged by size: 6691 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2380 2380 Size: 8236 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8236 PsiI TTA'TAA Cuts at: 5806 5806 Size: 8236 PspOMI G'GGCC_C Cuts at: 4664 4664 Size: 8236 PspXI vC'TCGA_Gb Cuts at: 5421 5421 Size: 8236 PstI C_TGCA'G Cuts at: 830 3063 3779 5588 830 Size: 2233 716 1809 3478 Fragments arranged by size: 3478 2233 1809 716 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8236 PvuII CAG'CTG Cuts at: 2509 3991 4466 5060 2509 Size: 1482 475 594 5685 Fragments arranged by size: 5685 1482 594 475 SacI G_AGCT'C Cuts at: 3267 3867 4360 4593 3267 Size: 600 493 233 6910 Fragments arranged by size: 6910 600 493 233 SalI G'TCGA_C Cuts at: 1936 5578 1936 Size: 3642 4594 SbfI CC_TGCA'GG Cuts at: 5588 5588 Size: 8236 ScaI AGT'ACT Cuts at: 6484 8196 6484 Size: 1712 6524 SfcI C'TryA_G Cuts at: 826 1045 3059 3287 3775 4348 5584 6719 7400 Size: 219 2014 228 488 573 1236 1135 681 Cuts at: 7400 7591 826 Size: 191 1471 Fragments arranged by size: 2014 1471 1236 1135 681 573 488 228 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8236 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8236 SmaI CCC'GGG Cuts at: 1872 5563 1872 Size: 3691 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2465 2894 2937 3002 5421 6340 Size: 197 926 522 429 43 65 2419 919 Cuts at: 6340 7241 7503 820 Size: 901 262 1553 Fragments arranged by size: 2419 1553 926 919 901 522 429 262 197 65 43 SnaBI TAC'GTA Cuts at: 493 6027 493 Size: 5534 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8236 SphI G_CATG'C Cuts at: 1132 2500 5594 1132 Size: 1368 3094 3774 Fragments arranged by size: 3774 3094 1368 SspI AAT'ATT Cuts at: 5 52 6160 5 Size: 47 6108 2081 Fragments arranged by size: 6108 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8236 StyI C'CwwG_G Cuts at: 513 1065 2020 4616 5097 5675 513 Size: 552 955 2596 481 578 3074 Fragments arranged by size: 3074 2596 955 578 552 481 SwaI ATTT'AAAT Cuts at: 3366 3366 Size: 8236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3368 4707 5488 6263 6280 6433 6618 Size: 1255 1788 1339 781 775 17 153 185 Cuts at: 6618 7063 325 Size: 445 1498 Fragments arranged by size: 1788 1498 1339 1255 781 775 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2806 4174 4252 Size: 274 80 33 51 1443 829 1368 78 Cuts at: 4252 6482 8194 96 Size: 2230 1712 138 Fragments arranged by size: 2230 1712 1443 1368 829 274 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4813 4845 5236 5268 5467 5499 8196 8228 4813 Size: 32 391 32 199 32 2697 32 4821 Fragments arranged by size: 4821 2697 391 199 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5038 1316 Size: 3722 4514 XbaI T'CTAG_A Cuts at: 4561 5572 4561 Size: 1011 7225 XhoI C'TCGA_G Cuts at: 1943 5421 1943 Size: 3478 4758 XmaI C'CCGG_G Cuts at: 1870 5561 1870 Size: 3691 4545 XmnI GAAnn'nnTTC Cuts at: 3078 3150 4484 4675 6365 3078 Size: 72 1334 191 1690 4949 Fragments arranged by size: 4949 1690 1334 191 72 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4407 6044 276 Size: 53 83 186 936 2873 1637 2468 Fragments arranged by size: 2873 2468 1637 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI