(Circular) (Six-base) MAPSORT of: pFN21AA0806.seq Check: 1131 from: 1 to: 7900 pFN21AA0806 7900 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3981 5710 278 Size: 53 83 186 936 2445 1729 2468 Fragments arranged by size: 2468 2445 1729 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5243 1937 Size: 3306 4594 Acc65I G'GTAC_C Cuts at: 4694 5221 4694 Size: 527 7373 AclI AA'CG_TT Cuts at: 1563 6027 6400 1563 Size: 4464 373 3063 Fragments arranged by size: 4464 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3071 5963 7347 7615 1632 Size: 1439 2892 1384 268 1917 Fragments arranged by size: 2892 1917 1439 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2725 2765 3100 820 Size: 197 1708 40 335 5620 Fragments arranged by size: 5620 1708 335 197 40 AflIII A'CryG_T Cuts at: 1203 6799 7776 1203 Size: 5596 977 1327 Fragments arranged by size: 5596 1327 977 AgeI A'CCGG_T Cuts at: 6731 6731 Size: 7900 AhdI GACnn_n'nnGTC Cuts at: 6629 6629 Size: 7900 AleI CACnn'nnGTG Cuts at: 1166 3566 1166 Size: 2400 5500 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2146 2201 1184 Size: 962 55 6883 Fragments arranged by size: 6883 962 55 AlwNI CAG_nnn'CTG Cuts at: 2044 4804 5643 7215 2044 Size: 2760 839 1572 2729 Fragments arranged by size: 2760 2729 1572 839 ApaLI G'TGCA_C Cuts at: 3417 3559 5957 7113 3417 Size: 142 2398 1156 4204 Fragments arranged by size: 4204 2398 1156 142 ApoI r'AATT_y Cuts at: 1493 2521 3025 3114 3643 3715 5209 5435 5587 Size: 1028 504 89 529 72 1494 226 152 Cuts at: 5587 7696 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1494 1028 529 504 226 152 89 72 AseI AT'TA_AT Cuts at: 160 1032 6454 160 Size: 872 5422 1606 Fragments arranged by size: 5422 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7900 AvaI C'yCGr_G Cuts at: 1870 1943 3942 3968 4128 4698 4749 5225 1870 Size: 73 1999 26 160 570 51 476 4545 Fragments arranged by size: 4545 1999 570 476 160 73 51 26 AvrII C'CTAG_G Cuts at: 2020 2020 Size: 7900 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4504 4537 4686 4719 4504 Size: 33 149 33 7685 Fragments arranged by size: 7685 149 33 33 BamHI G'GATC_C Cuts at: 5230 5597 5230 Size: 367 7533 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2014 3851 4694 5221 Size: 325 217 627 221 6 1837 843 527 Cuts at: 5221 6676 618 Size: 1455 1842 Fragments arranged by size: 1842 1837 1455 843 627 527 325 221 217 6 BanII G_rGCy'C Cuts at: 1391 4542 5219 1391 Size: 3151 677 4072 Fragments arranged by size: 4072 3151 677 BbeI G_GCGC'C Cuts at: 2012 2018 2012 Size: 6 7894 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3973 4518 4566 928 Size: 971 2074 545 48 4262 Fragments arranged by size: 4262 2074 971 545 48 BbvCI CC'TCA_GC Cuts at: 2456 4779 2456 Size: 2323 5577 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6091 6125 1903 Size: 34 4154 34 3678 Fragments arranged by size: 4154 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3628 4855 5792 7002 3628 Size: 1227 937 1210 4526 Fragments arranged by size: 4526 1227 1210 937 BclI T'GATC_A Cuts at: 1548 4795 1548 Size: 3247 4653 BfrBI ATG'CAT Cuts at: 2921 2921 Size: 7900 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6511 136 Size: 107 122 71 631 605 4839 1525 Fragments arranged by size: 4839 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2634 2729 7895 2634 Size: 95 5166 2639 Fragments arranged by size: 5166 2639 95 BlpI GC'TnA_GC Cuts at: 5317 5317 Size: 7900 Bme1580I G_kGCm'C Cuts at: 1790 3421 3563 5198 5961 7117 1790 Size: 1631 142 1635 763 1156 2573 Fragments arranged by size: 2573 1635 1631 1156 763 142 BmgBI CAC'GTC Cuts at: 1318 3558 3631 3961 1318 Size: 2240 73 330 5257 Fragments arranged by size: 5257 2240 330 73 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5631 6589 442 Size: 416 53 83 399 24 4214 958 1753 Fragments arranged by size: 4214 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3437 1056 Size: 2381 5519 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2144 4108 2144 Size: 1964 5936 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2221 2408 2462 3690 4130 4182 4726 6560 Size: 982 187 54 1228 440 52 544 1834 Cuts at: 6560 1239 Size: 2579 Fragments arranged by size: 2579 1834 1228 982 544 440 187 54 52 Bpu10I CC'TnA_GC Cuts at: 2456 4779 2456 Size: 2323 5577 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3260 3476 6025 6890 7188 3260 Size: 216 2549 865 298 3972 Fragments arranged by size: 3972 2549 865 298 216 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2132 4058 6563 882 Size: 617 633 1926 2505 2219 Fragments arranged by size: 2505 2219 1926 633 617 BsaAI yAC'GTr Cuts at: 493 1204 5691 493 Size: 711 4487 2702 Fragments arranged by size: 4487 2702 711 BsaBI GATnn'nnATC Cuts at: 7894 7894 Size: 7900 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2015 2025 3978 Size: 53 83 186 936 476 6 10 1953 Cuts at: 3978 5707 6089 6724 275 Size: 1729 382 635 1451 Fragments arranged by size: 1953 1729 1451 936 635 476 382 186 83 53 10 6 BsaWI w'CCGG_w Cuts at: 6333 6731 7005 7152 7563 6333 Size: 398 274 147 411 6670 Fragments arranged by size: 6670 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 2406 2436 4724 Size: 30 165 30 621 30 352 30 2288 Cuts at: 4724 4754 1178 Size: 30 4324 Fragments arranged by size: 4324 2288 621 352 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2090 4550 4796 1186 Size: 862 42 2460 246 4290 Fragments arranged by size: 4290 2460 862 246 42 BseYI C'CCAG_C Cuts at: 2044 4286 4562 5040 7103 7599 2044 Size: 2242 276 478 2063 496 2345 Fragments arranged by size: 2345 2242 2063 496 478 276 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3876 1163 Size: 573 2140 5187 Fragments arranged by size: 5187 2140 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5350 6111 7139 1540 Size: 275 186 3349 761 1028 2301 Fragments arranged by size: 3349 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3421 3563 4542 5961 6046 7117 3421 Size: 142 979 1419 85 1071 4204 Fragments arranged by size: 4204 1419 1071 979 142 85 BsmI GAATG_Cn' Cuts at: 2154 2899 3218 5411 5504 2154 Size: 745 319 2193 93 4550 Fragments arranged by size: 4550 2193 745 319 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4187 4187 Size: 7900 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3421 3563 4542 5198 5219 5961 6046 Size: 399 1631 142 979 656 21 742 85 Cuts at: 6046 7117 1391 Size: 1071 2174 Fragments arranged by size: 2174 1631 1071 979 742 656 399 142 85 21 BspEI T'CCGG_A Cuts at: 7563 7563 Size: 7900 BspHI T'CATG_A Cuts at: 5789 7519 5789 Size: 1730 6170 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2114 4307 5255 844 Size: 1270 2193 948 3489 Fragments arranged by size: 3489 2193 1270 948 BsrBI CCG'CTC Cuts at: 1394 1823 2096 4971 5787 1394 Size: 429 273 2875 816 3507 Fragments arranged by size: 3507 2875 816 429 273 BsrDI GCAATG_nn' Cuts at: 65 1248 2082 4979 6395 6569 65 Size: 1183 834 2897 1416 174 1396 Fragments arranged by size: 2897 1416 1396 1183 834 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2011 6544 6731 1700 Size: 110 201 4533 187 2869 Fragments arranged by size: 4533 2869 201 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7900 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7900 BssSI C'ACGA_G Cuts at: 2832 5960 6972 2832 Size: 3128 1012 3760 Fragments arranged by size: 3760 3128 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3964 4645 794 Size: 3170 681 4049 Fragments arranged by size: 4049 3170 681 BstBI TT'CG_AA Cuts at: 6704 6704 Size: 7900 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7900 BstXI CCAn_nnnn'nTGG Cuts at: 4816 4816 Size: 7900 BstYI r'GATC_y Cuts at: 1972 2634 2729 3460 3832 5230 5597 5982 5999 Size: 662 95 731 372 1398 367 385 17 Cuts at: 5999 7451 7537 7549 7895 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1398 731 662 385 372 367 346 95 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3855 513 Size: 552 939 1851 4558 Fragments arranged by size: 4558 1851 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7161 Fragments arranged by size: 7161 683 56 BtsI GCAGTG_nn' Cuts at: 3561 3573 3583 5416 6210 6230 3561 Size: 12 10 1833 794 20 5231 Fragments arranged by size: 5231 1833 794 20 12 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7900 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7865 DraI TTT'AAA Cuts at: 3309 5204 3309 Size: 1895 6005 DraIII CAC_nnn'GTG Cuts at: 3600 3600 Size: 7900 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3041 3162 6907 809 Size: 507 1725 121 3745 1802 Fragments arranged by size: 3745 1802 1725 507 121 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3858 4333 5347 Size: 54 1425 50 275 18 2028 475 1014 Cuts at: 5347 6236 7865 8 Size: 889 1629 43 Fragments arranged by size: 2028 1629 1425 1014 889 475 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5347 1537 Size: 275 3535 4090 Fragments arranged by size: 4090 3535 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 4381 5830 1353 Size: 731 2297 1449 3423 Fragments arranged by size: 3423 2297 1449 731 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6487 6861 7007 387 Size: 1064 138 4898 374 146 1280 Fragments arranged by size: 4898 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4540 4540 Size: 7900 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2221 2408 2462 3071 3690 4130 4182 Size: 393 589 187 54 609 619 440 52 Cuts at: 4182 4726 5963 6560 7347 7615 1239 Size: 544 1237 597 787 268 1524 Fragments arranged by size: 1524 1237 787 619 609 597 589 544 440 393 268 187 54 52 EcoNI CCTnn'n_nnAGG Cuts at: 5131 5131 Size: 7900 EcoO109I rG'GnC_Cy Cuts at: 4746 5083 4746 Size: 337 7563 EcoRI G'AATT_C Cuts at: 5209 5587 5209 Size: 378 7522 FspI TGC'GCA Cuts at: 6406 6406 Size: 7900 HaeII r_GCGC'y Cuts at: 1678 2012 2018 2210 7047 1678 Size: 334 6 192 4837 2531 Fragments arranged by size: 4837 2531 334 192 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 2558 2590 3794 Size: 32 135 32 872 32 82 32 1204 Cuts at: 3794 3826 6541 6573 6615 6647 1373 Size: 32 2715 32 42 32 2626 Fragments arranged by size: 2715 2626 1204 872 135 82 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5244 5490 669 Size: 988 281 3306 246 3079 Fragments arranged by size: 3306 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7900 HpaI GTT'AAC Cuts at: 5490 5490 Size: 7900 KasI G'GCGC_C Cuts at: 2008 2014 2008 Size: 6 7894 KpnI G_GTAC'C Cuts at: 4698 5225 4698 Size: 527 7373 MfeI C'AATT_G Cuts at: 4366 5499 4366 Size: 1133 6767 MluI A'CGCG_T Cuts at: 7776 7776 Size: 7900 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7014 7198 1305 Size: 5709 184 2007 Fragments arranged by size: 5709 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3860 4335 10 Size: 54 1425 343 2028 475 3575 Fragments arranged by size: 3575 2028 1425 475 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3545 3566 4401 4439 4964 5076 5858 Size: 648 2379 21 835 38 525 112 782 Cuts at: 5858 6217 6376 518 Size: 359 159 2042 Fragments arranged by size: 2379 2042 835 782 648 525 359 159 112 38 21 MspA1I CmG'CkG Cuts at: 1606 2746 2971 4987 5316 5993 7141 7386 1606 Size: 1140 225 2016 329 677 1148 245 2120 Fragments arranged by size: 2120 2016 1148 1140 677 329 245 225 NaeI GCC'GGC Cuts at: 2013 2013 Size: 7900 NarI GG'CG_CC Cuts at: 2009 2015 2009 Size: 6 7894 NcoI C'CATG_G Cuts at: 513 1065 2004 3855 513 Size: 552 939 1851 4558 Fragments arranged by size: 4558 1851 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7900 NgoMIV G'CCGG_C Cuts at: 2011 2011 Size: 7900 NheI G'CTAG_C Cuts at: 1052 3433 1052 Size: 2381 5519 NotI GC'GGCC_GC Cuts at: 5347 5347 Size: 7900 NsiI A_TGCA'T Cuts at: 2923 2923 Size: 7900 NspI r_CATG'y Cuts at: 1132 1229 2506 3958 5258 6803 1132 Size: 97 1277 1452 1300 1545 2229 Fragments arranged by size: 2229 1545 1452 1300 1277 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7900 PciI A'CATG_T Cuts at: 6799 6799 Size: 7900 PflMI CCAn_nnn'nTGG Cuts at: 1108 2662 3600 1108 Size: 1554 938 5408 Fragments arranged by size: 5408 1554 938 PfoI T'CCnGG_A Cuts at: 2028 2163 2241 4742 2028 Size: 135 78 2501 5186 Fragments arranged by size: 5186 2501 135 78 PmeI GTTT'AAAC Cuts at: 5204 5204 Size: 7900 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5969 6001 7482 7514 5969 Size: 32 1481 32 6355 Fragments arranged by size: 6355 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4746 4746 Size: 7900 PshAI GACnn'nnGTC Cuts at: 1613 2172 1613 Size: 559 7341 PsiI TTA'TAA Cuts at: 5470 5470 Size: 7900 PstI C_TGCA'G Cuts at: 830 2174 3568 3880 4243 4555 5252 830 Size: 1344 1394 312 363 312 697 3478 Fragments arranged by size: 3478 1394 1344 697 363 312 312 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7900 PvuII CAG'CTG Cuts at: 2971 2971 Size: 7900 SacI G_AGCT'C Cuts at: 4542 4542 Size: 7900 SalI G'TCGA_C Cuts at: 1936 5242 1936 Size: 3306 4594 SbfI CC_TGCA'GG Cuts at: 5252 5252 Size: 7900 ScaI AGT'ACT Cuts at: 6148 7860 6148 Size: 1712 6188 SfcI C'TryA_G Cuts at: 826 1045 2170 3564 3876 4021 4239 4516 4551 Size: 219 1125 1394 312 145 218 277 35 Cuts at: 4551 4609 5248 6383 7064 7255 826 Size: 58 639 1135 681 191 1471 Fragments arranged by size: 1471 1394 1135 1125 681 639 312 277 219 218 191 145 58 35 SfoI GGC'GCC Cuts at: 2010 2016 2010 Size: 6 7894 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7900 SgrAI Cr'CCGG_yG Cuts at: 1700 2011 1700 Size: 311 7589 SmaI CCC'GGG Cuts at: 1872 4700 5227 1872 Size: 2828 527 4545 Fragments arranged by size: 4545 2828 527 SmlI C'TyrA_G Cuts at: 820 1017 1943 2725 2765 3100 3239 3491 3942 Size: 197 926 782 40 335 139 252 451 Cuts at: 3942 6004 6905 7167 820 Size: 2062 901 262 1553 Fragments arranged by size: 2062 1553 926 901 782 451 335 262 252 197 139 40 SnaBI TAC'GTA Cuts at: 493 5691 493 Size: 5198 2702 SpeI A'CTAG_T Cuts at: 152 3689 152 Size: 3537 4363 SphI G_CATG'C Cuts at: 1132 2506 3958 5258 1132 Size: 1374 1452 1300 3774 Fragments arranged by size: 3774 1452 1374 1300 SspI AAT'ATT Cuts at: 5 52 2368 5824 5 Size: 47 2316 3456 2081 Fragments arranged by size: 3456 2316 2081 47 StuI AGG'CCT Cuts at: 1517 5019 1517 Size: 3502 4398 StyI C'CwwG_G Cuts at: 513 1065 2004 2020 2547 2710 3855 4318 4579 Size: 552 939 16 527 163 1145 463 261 Cuts at: 4579 5339 513 Size: 760 3074 Fragments arranged by size: 3074 1145 939 760 552 527 463 261 163 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3601 5927 5944 6097 6282 6727 325 Size: 1255 2021 2326 17 153 185 445 1498 Fragments arranged by size: 2326 2021 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4846 6146 7858 Size: 274 80 33 51 1443 2869 1300 1712 Cuts at: 7858 96 Size: 138 Fragments arranged by size: 2869 1712 1443 1300 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2406 2438 4315 4347 7860 7892 2406 Size: 32 1877 32 3513 32 2414 Fragments arranged by size: 3513 2414 1877 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4804 1316 Size: 3488 4412 XbaI T'CTAG_A Cuts at: 2057 3247 5236 2057 Size: 1190 1989 4721 Fragments arranged by size: 4721 1989 1190 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4388 4388 Size: 7900 XhoI C'TCGA_G Cuts at: 1943 3942 1943 Size: 1999 5901 XmaI C'CCGG_G Cuts at: 1870 4698 5225 1870 Size: 2828 527 4545 Fragments arranged by size: 4545 2828 527 XmnI GAAnn'nnTTC Cuts at: 3218 3318 6029 3218 Size: 100 2711 5089 Fragments arranged by size: 5089 2711 100 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3979 5708 276 Size: 53 83 186 936 2445 1729 2468 Fragments arranged by size: 2468 2445 1729 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI