(Circular) (Six-base) MAPSORT of: pFN21AA0726.seq Check: 3459 from: 1 to: 7573 pFN21AA0726 7573 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2180 3052 4031 2180 Size: 872 979 5722 Fragments arranged by size: 5722 979 872 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5383 278 Size: 53 83 186 936 3847 2468 Fragments arranged by size: 3847 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4916 1937 Size: 2979 4594 Acc65I G'GTAC_C Cuts at: 4894 4894 Size: 7573 AclI AA'CG_TT Cuts at: 1563 5700 6073 1563 Size: 4137 373 3063 Fragments arranged by size: 4137 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3841 3896 3906 5636 7020 7288 1632 Size: 2209 55 10 1730 1384 268 1917 Fragments arranged by size: 2209 1917 1730 1384 268 55 10 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7376 AflIII A'CryG_T Cuts at: 1203 6472 7449 1203 Size: 5269 977 1327 Fragments arranged by size: 5269 1327 977 AgeI A'CCGG_T Cuts at: 6404 6404 Size: 7573 AhdI GACnn_n'nnGTC Cuts at: 3553 6302 3553 Size: 2749 4824 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7573 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7573 AlwNI CAG_nnn'CTG Cuts at: 2780 3727 3934 4462 5316 6888 2780 Size: 947 207 528 854 1572 3465 Fragments arranged by size: 3465 1572 947 854 528 207 ApaI G_GGCC'C Cuts at: 2357 3102 2357 Size: 745 6828 ApaLI G'TGCA_C Cuts at: 3005 4109 5630 6786 3005 Size: 1104 1521 1156 3792 Fragments arranged by size: 3792 1521 1156 1104 ApoI r'AATT_y Cuts at: 1493 4131 4382 4882 5108 5260 7369 1493 Size: 2638 251 500 226 152 2109 1697 Fragments arranged by size: 2638 2109 1697 500 251 226 152 AseI AT'TA_AT Cuts at: 160 1032 6127 160 Size: 872 5095 1606 Fragments arranged by size: 5095 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 AvaI C'yCGr_G Cuts at: 1870 1943 3365 4160 4898 1870 Size: 73 1422 795 738 4545 Fragments arranged by size: 4545 1422 795 738 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2435 2468 2435 Size: 33 7540 BamHI G'GATC_C Cuts at: 4156 4903 5270 4156 Size: 747 367 6459 Fragments arranged by size: 6459 747 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2227 3047 3064 3345 4894 Size: 325 217 627 440 820 17 281 1549 Cuts at: 4894 6349 618 Size: 1455 1842 Fragments arranged by size: 1842 1549 1455 820 627 440 325 281 217 17 BanII G_rGCy'C Cuts at: 1391 2357 3095 3102 4218 4892 1391 Size: 966 738 7 1116 674 4072 Fragments arranged by size: 4072 1116 966 738 674 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6602 BbvCI CC'TCA_GC Cuts at: 2700 2700 Size: 7573 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2982 3016 3388 3422 5764 5798 1903 Size: 34 1045 34 372 34 2342 34 3678 Fragments arranged by size: 3678 2342 1045 372 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2816 5465 6675 2816 Size: 2649 1210 3714 Fragments arranged by size: 3714 2649 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7573 BfrBI ATG'CAT Cuts at: 3647 3647 Size: 7573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6184 136 Size: 107 122 71 631 605 4512 1525 Fragments arranged by size: 4512 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2196 2541 7568 2196 Size: 345 5027 2201 Fragments arranged by size: 5027 2201 345 BlpI GC'TnA_GC Cuts at: 4445 4990 4445 Size: 545 7028 Bme1580I G_kGCm'C Cuts at: 1790 2232 2357 3009 3069 3102 3295 4076 4113 Size: 442 125 652 60 33 193 781 37 Cuts at: 4113 5634 6790 1790 Size: 1521 1156 2573 Fragments arranged by size: 2573 1521 1156 781 652 442 193 125 60 37 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7573 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2426 2524 2952 Size: 416 53 83 399 24 1009 98 428 Cuts at: 2952 3075 3293 5304 6262 442 Size: 123 218 2011 958 1753 Fragments arranged by size: 2011 1753 1009 958 428 416 399 218 123 98 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7573 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4308 4337 4308 Size: 29 7544 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2834 3341 3635 3758 4232 4357 4638 4648 Size: 1595 507 294 123 474 125 281 10 Cuts at: 4648 6233 1239 Size: 1585 2579 Fragments arranged by size: 2579 1595 1585 507 474 294 281 125 123 10 Bpu10I CC'TnA_GC Cuts at: 2700 3604 2700 Size: 904 6669 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3305 5698 6563 6861 3305 Size: 2393 865 298 4017 Fragments arranged by size: 4017 2393 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2810 3540 3749 4847 6236 882 Size: 617 1311 730 209 1098 1389 2219 Fragments arranged by size: 2219 1389 1311 1098 730 617 209 BsaAI yAC'GTr Cuts at: 493 1204 3709 3901 4441 5364 493 Size: 711 2505 192 540 923 2702 Fragments arranged by size: 2702 2505 923 711 540 192 BsaBI GATnn'nnATC Cuts at: 7567 7567 Size: 7573 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3819 3921 3993 5380 Size: 53 83 186 936 2286 102 72 1387 Cuts at: 5380 5762 6397 275 Size: 382 635 1451 Fragments arranged by size: 2286 1451 1387 936 635 382 186 102 83 72 53 BsaWI w'CCGG_w Cuts at: 6006 6404 6678 6825 7236 6006 Size: 398 274 147 411 6343 Fragments arranged by size: 6343 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3386 3416 4511 4541 1178 Size: 30 165 30 1983 30 1095 30 4210 Fragments arranged by size: 4210 1983 1095 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3506 1186 Size: 2320 5253 BseYI C'CCAG_C Cuts at: 2735 2780 3088 3964 4247 4433 4452 4538 4826 Size: 45 308 876 283 186 19 86 288 Cuts at: 4826 6776 7272 2735 Size: 1950 496 3036 Fragments arranged by size: 3036 1950 876 496 308 288 283 186 86 45 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3080 4391 4448 1163 Size: 573 1344 1311 57 4288 Fragments arranged by size: 4288 1344 1311 573 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2488 5023 5784 6812 1540 Size: 275 186 487 2535 761 1028 2301 Fragments arranged by size: 2535 2301 1028 761 487 275 186 BsiHKAI G_wGCw'C Cuts at: 3009 4113 4218 4446 4587 5634 5719 6790 3009 Size: 1104 105 228 141 1047 85 1071 3792 Fragments arranged by size: 3792 1104 1071 1047 228 141 105 85 BsmI GAATG_Cn' Cuts at: 5084 5177 5084 Size: 93 7480 BsmBI CGTCTCn'nnnn_ Cuts at: 3880 4639 4834 3880 Size: 759 195 6619 Fragments arranged by size: 6619 759 195 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2357 3009 3069 3095 3102 3295 Size: 399 442 125 652 60 26 7 193 Cuts at: 3295 4076 4113 4218 4446 4587 4892 5634 5719 Size: 781 37 105 228 141 305 742 85 Cuts at: 5719 6790 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 781 742 652 442 399 305 228 193 141 125 105 85 60 37 26 7 BspEI T'CCGG_A Cuts at: 7236 7236 Size: 7573 BspHI T'CATG_A Cuts at: 3468 5462 7192 3468 Size: 1994 1730 3849 Fragments arranged by size: 3849 1994 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2180 3052 3316 4031 4928 844 Size: 1336 872 264 715 897 3489 Fragments arranged by size: 3489 1336 897 872 715 264 BsrBI CCG'CTC Cuts at: 1394 1823 2911 4165 5460 1394 Size: 429 1088 1254 1295 3507 Fragments arranged by size: 3507 1295 1254 1088 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4384 6068 6242 65 Size: 1183 3136 1684 174 1396 Fragments arranged by size: 3136 1684 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4643 6217 6404 1700 Size: 110 2833 1574 187 2869 Fragments arranged by size: 2869 2833 1574 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7573 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7573 BssSI C'ACGA_G Cuts at: 5633 6645 5633 Size: 1012 6561 BstAPI GCAn_nnn'nTGC Cuts at: 794 2696 2777 4462 794 Size: 1902 81 1685 3905 Fragments arranged by size: 3905 1902 1685 81 BstBI TT'CG_AA Cuts at: 6377 6377 Size: 7573 BstEII G'GTnAC_C Cuts at: 1184 4472 1184 Size: 3288 4285 BstXI CCAn_nnnn'nTGG Cuts at: 2222 2875 3088 3431 2222 Size: 653 213 343 6364 Fragments arranged by size: 6364 653 343 213 BstYI r'GATC_y Cuts at: 1972 2196 2541 2953 3930 4156 4278 4903 5270 Size: 224 345 412 977 226 122 625 367 Cuts at: 5270 5655 5672 7124 7210 7222 7568 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 977 625 412 385 367 346 345 226 224 122 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4283 4283 Size: 7573 BtgI C'CryG_G Cuts at: 513 1065 2084 2215 3247 3574 4468 4728 513 Size: 552 1019 131 1032 327 894 260 3358 Fragments arranged by size: 3358 1032 1019 894 552 327 260 131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6834 Fragments arranged by size: 6834 683 56 BtsI GCAGTG_nn' Cuts at: 3840 3945 4807 5089 5883 5903 3840 Size: 105 862 282 794 20 5510 Fragments arranged by size: 5510 862 794 282 105 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7573 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7538 DraI TTT'AAA Cuts at: 4877 4877 Size: 7573 DraIII CAC_nnn'GTG Cuts at: 2777 4042 2777 Size: 1265 6308 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2132 6580 809 Size: 507 816 4448 1802 Fragments arranged by size: 4448 1802 816 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2877 4363 4815 Size: 54 1425 50 275 18 1047 1486 452 Cuts at: 4815 5020 5909 7538 8 Size: 205 889 1629 43 Fragments arranged by size: 1629 1486 1425 1047 889 452 275 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5020 1537 Size: 275 3208 4090 Fragments arranged by size: 4090 3208 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3174 3858 4269 4694 5503 1353 Size: 1821 684 411 425 809 3423 Fragments arranged by size: 3423 1821 809 684 425 411 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2798 6160 6534 6680 387 Size: 1064 138 1209 3362 374 146 1280 Fragments arranged by size: 3362 1280 1209 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4216 4216 Size: 7573 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2834 3341 3635 3758 3841 3896 3906 Size: 393 1202 507 294 123 83 55 10 Cuts at: 3906 4232 4357 4638 4648 5636 6233 7020 7288 Size: 326 125 281 10 988 597 787 268 Cuts at: 7288 1239 Size: 1524 Fragments arranged by size: 1524 1202 988 787 597 507 393 326 294 281 268 125 123 83 55 10 10 EcoNI CCTnn'n_nnAGG Cuts at: 2252 4286 2252 Size: 2034 5539 EcoO109I rG'GnC_Cy Cuts at: 2353 2354 3099 3477 3825 4659 4678 2353 Size: 1 745 378 348 834 19 5248 Fragments arranged by size: 5248 834 745 378 348 19 1 EcoRI G'AATT_C Cuts at: 4382 4882 5260 4382 Size: 500 378 6695 Fragments arranged by size: 6695 500 378 FspI TGC'GCA Cuts at: 4610 6079 4610 Size: 1469 6104 FspAI rTGC'GCAy Cuts at: 4610 4610 Size: 7573 HaeII r_GCGC'y Cuts at: 1678 2918 4545 6720 1678 Size: 1240 1627 2175 2531 Fragments arranged by size: 2531 2175 1627 1240 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2888 2920 3209 3241 3386 Size: 32 135 32 1316 32 289 32 145 Cuts at: 3386 3418 3539 3571 4175 4207 4833 4865 6214 Size: 32 121 32 604 32 626 32 1349 Cuts at: 6214 6246 6288 6320 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1349 1316 626 604 289 145 135 121 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2410 4917 5163 669 Size: 988 281 472 2507 246 3079 Fragments arranged by size: 3079 2507 988 472 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7573 HpaI GTT'AAC Cuts at: 5163 5163 Size: 7573 KpnI G_GTAC'C Cuts at: 4898 4898 Size: 7573 MfeI C'AATT_G Cuts at: 5172 5172 Size: 7573 MluI A'CGCG_T Cuts at: 7449 7449 Size: 7573 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4077 6687 6871 1305 Size: 2772 2610 184 2007 Fragments arranged by size: 2772 2610 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2879 10 Size: 54 1425 343 1047 4704 Fragments arranged by size: 4704 1425 1047 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 3429 4091 4265 4559 4562 4718 Size: 648 948 1315 662 174 294 3 156 Cuts at: 4718 5531 5890 6049 518 Size: 813 359 159 2042 Fragments arranged by size: 2042 1315 948 813 662 648 359 294 174 159 156 3 MspA1I CmG'CkG Cuts at: 1606 2179 2739 3968 4204 4989 5666 6814 7059 Size: 573 560 1229 236 785 677 1148 245 Cuts at: 7059 1606 Size: 2120 Fragments arranged by size: 2120 1229 1148 785 677 573 560 245 236 NaeI GCC'GGC Cuts at: 4645 4645 Size: 7573 NcoI C'CATG_G Cuts at: 513 1065 2084 2215 3574 4728 513 Size: 552 1019 131 1359 1154 3358 Fragments arranged by size: 3358 1359 1154 1019 552 131 NdeI CA'TA_TG Cuts at: 387 387 Size: 7573 NgoMIV G'CCGG_C Cuts at: 4643 4643 Size: 7573 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7573 NotI GC'GGCC_GC Cuts at: 5020 5020 Size: 7573 NsiI A_TGCA'T Cuts at: 3649 3649 Size: 7573 NspI r_CATG'y Cuts at: 1132 1229 2402 3651 3782 4096 4931 6476 1132 Size: 97 1173 1249 131 314 835 1545 2229 Fragments arranged by size: 2229 1545 1249 1173 835 314 131 97 PasI CC'CwG_GG Cuts at: 1348 3070 1348 Size: 1722 5851 PciI A'CATG_T Cuts at: 6472 6472 Size: 7573 PflMI CCAn_nnn'nTGG Cuts at: 1108 2780 1108 Size: 1672 5901 PfoI T'CCnGG_A Cuts at: 4179 4179 Size: 7573 PmeI GTTT'AAAC Cuts at: 4877 4877 Size: 7573 PmlI CAC'GTG Cuts at: 3709 4441 3709 Size: 732 6841 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5642 5674 7155 7187 5642 Size: 32 1481 32 6028 Fragments arranged by size: 6028 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4678 4678 Size: 7573 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7573 PsiI TTA'TAA Cuts at: 5143 5143 Size: 7573 PspOMI G'GGCC_C Cuts at: 2353 3098 2353 Size: 745 6828 PspXI vC'TCGA_Gb Cuts at: 3365 3365 Size: 7573 PstI C_TGCA'G Cuts at: 830 2625 2865 3777 4200 4925 830 Size: 1795 240 912 423 725 3478 Fragments arranged by size: 3478 1795 912 725 423 240 PvuI CG_AT'CG Cuts at: 2001 2488 2001 Size: 487 7086 PvuII CAG'CTG Cuts at: 2739 3968 2739 Size: 1229 6344 SacI G_AGCT'C Cuts at: 4218 4218 Size: 7573 SalI G'TCGA_C Cuts at: 1936 4915 1936 Size: 2979 4594 SbfI CC_TGCA'GG Cuts at: 4925 4925 Size: 7573 ScaI AGT'ACT Cuts at: 5821 7533 5821 Size: 1712 5861 SexAI A'CCwGG_T Cuts at: 2773 4038 2773 Size: 1265 6308 SfcI C'TryA_G Cuts at: 826 1045 2621 2861 3010 3253 3667 3773 4196 Size: 219 1576 240 149 243 414 106 423 Cuts at: 4196 4921 6056 6737 6928 826 Size: 725 1135 681 191 1471 Fragments arranged by size: 1576 1471 1135 725 681 423 414 243 240 219 191 149 106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7573 SmaI CCC'GGG Cuts at: 1872 4900 1872 Size: 3028 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3284 3365 5677 6578 6840 820 Size: 197 926 1341 81 2312 901 262 1553 Fragments arranged by size: 2312 1553 1341 926 901 262 197 81 SnaBI TAC'GTA Cuts at: 493 5364 493 Size: 4871 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7573 SphI G_CATG'C Cuts at: 1132 4931 1132 Size: 3799 3774 SspI AAT'ATT Cuts at: 5 52 5497 5 Size: 47 5445 2081 Fragments arranged by size: 5445 2081 47 StuI AGG'CCT Cuts at: 1517 2336 1517 Size: 819 6754 StyI C'CwwG_G Cuts at: 513 1065 2084 2160 2215 2283 2880 3541 3574 Size: 552 1019 76 55 68 597 661 33 Cuts at: 3574 4674 4728 5012 513 Size: 1100 54 284 3074 Fragments arranged by size: 3074 1100 1019 661 597 552 284 76 68 55 54 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2581 3062 3065 3459 3554 5600 5617 Size: 1255 1001 481 3 394 95 2046 17 Cuts at: 5617 5770 5955 6400 325 Size: 153 185 445 1498 Fragments arranged by size: 2046 1498 1255 1001 481 445 394 185 153 95 17 3 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2532 2625 3527 Size: 274 80 33 51 1443 555 93 902 Cuts at: 3527 5819 7531 96 Size: 2292 1712 138 Fragments arranged by size: 2292 1712 1443 902 555 274 138 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2914 2946 2988 3020 4511 4543 7533 7565 2914 Size: 32 42 32 1491 32 2990 32 2922 Fragments arranged by size: 2990 2922 1491 42 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7573 XbaI T'CTAG_A Cuts at: 4909 4909 Size: 7573 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2290 2947 3572 3886 4689 2290 Size: 657 625 314 803 5174 Fragments arranged by size: 5174 803 657 625 314 XhoI C'TCGA_G Cuts at: 1943 3365 1943 Size: 1422 6151 XmaI C'CCGG_G Cuts at: 1870 4898 1870 Size: 3028 4545 XmnI GAAnn'nnTTC Cuts at: 5702 5702 Size: 7573 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5381 276 Size: 53 83 186 936 3847 2468 Fragments arranged by size: 3847 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BsiWI BstZ17I EcoRV FalI FseI KasI NarI NruI PacI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI