Restriction map for
pFN21AA0726
(Linear) (Six-base) MAPSORT of: KIAA0726.seq Check: 6918 from: 1 to: 2868
KIAA0726.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 175 1047 2026 2868
Size: 175 872 979 842
Fragments arranged by size:
979 872 842 175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1836 1891 1901 2868
Size: 1836 55 10 967
Fragments arranged by size:
1836 967 55 10
AhdI GACnn_n'nnGTC
Cuts at: 0 1548 2868
Size: 1548 1320
AlwNI CAG_nnn'CTG
Cuts at: 0 775 1722 1929 2457 2868
Size: 775 947 207 528 411
Fragments arranged by size:
947 775 528 411 207
ApaI G_GGCC'C
Cuts at: 0 352 1097 2868
Size: 352 745 1771
Fragments arranged by size:
1771 745 352
ApaLI G'TGCA_C
Cuts at: 0 1000 2104 2868
Size: 1000 1104 764
Fragments arranged by size:
1104 1000 764
ApoI r'AATT_y
Cuts at: 0 2126 2377 2868
Size: 2126 251 491
Fragments arranged by size:
2126 491 251
AvaI C'yCGr_G
Cuts at: 0 1360 2155 2868
Size: 1360 795 713
Fragments arranged by size:
1360 795 713
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 430 463 2868
Size: 430 33 2405
Fragments arranged by size:
2405 430 33
BamHI G'GATC_C
Cuts at: 0 2151 2868
Size: 2151 717
BanI G'GyrC_C
Cuts at: 0 222 1042 1059 1340 2868
Size: 222 820 17 281 1528
Fragments arranged by size:
1528 820 281 222 17
BanII G_rGCy'C
Cuts at: 0 352 1090 1097 2213 2868
Size: 352 738 7 1116 655
Fragments arranged by size:
1116 738 655 352 7
BbvCI CC'TCA_GC
Cuts at: 0 695 2868
Size: 695 2173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 977 1011 1383 1417 2868
Size: 977 34 372 34 1451
Fragments arranged by size:
1451 977 372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 811 2868
Size: 811 2057
BfrBI ATG'CAT
Cuts at: 0 1642 2868
Size: 1642 1226
BglII A'GATC_T
Cuts at: 0 191 536 2868
Size: 191 345 2332
Fragments arranged by size:
2332 345 191
BlpI GC'TnA_GC
Cuts at: 0 2440 2868
Size: 2440 428
Bme1580I G_kGCm'C
Cuts at: 0 227 352 1004 1064 1097 1290 2071 2108
Size: 227 125 652 60 33 193 781 37
Cuts at: 2108 2868
Size: 760
Fragments arranged by size:
781 760 652 227 193 125 60 37
33
BmrI ACTGGGnnnn_n'
Cuts at: 0 421 519 947 1070 1288 2868
Size: 421 98 428 123 218 1580
Fragments arranged by size:
1580 428 421 218 123 98
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2303 2332 2868
Size: 2303 29 536
Fragments arranged by size:
2303 536 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1336 1630 1753 2227 2352 2633 2643
Size: 829 507 294 123 474 125 281 10
Cuts at: 2643 2868
Size: 225
Fragments arranged by size:
829 507 474 294 281 225 125 123
10
Bpu10I CC'TnA_GC
Cuts at: 0 695 1599 2868
Size: 695 904 1269
Fragments arranged by size:
1269 904 695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1300 2868
Size: 1300 1568
BsaI GGTCTCn'nnnn_
Cuts at: 0 805 1535 1744 2842 2868
Size: 805 730 209 1098 26
Fragments arranged by size:
1098 805 730 209 26
BsaAI yAC'GTr
Cuts at: 0 1704 1896 2436 2868
Size: 1704 192 540 432
Fragments arranged by size:
1704 540 432 192
BsaHI Gr'CG_yC
Cuts at: 0 1814 1916 1988 2868
Size: 1814 102 72 880
Fragments arranged by size:
1814 880 102 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1381 1411 2506 2536 2868
Size: 1381 30 1095 30 332
Fragments arranged by size:
1381 1095 332 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1501 2868
Size: 1501 1367
BseYI C'CCAG_C
Cuts at: 0 730 775 1083 1959 2242 2428 2447 2533
Size: 730 45 308 876 283 186 19 86
Cuts at: 2533 2821 2868
Size: 288 47
Fragments arranged by size:
876 730 308 288 283 186 86 47
45 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1075 2386 2443 2868
Size: 1075 1311 57 425
Fragments arranged by size:
1311 1075 425 57
BsiEI CG_ry'CG
Cuts at: 0 483 2868
Size: 483 2385
BsiHKAI G_wGCw'C
Cuts at: 0 1004 2108 2213 2441 2582 2868
Size: 1004 1104 105 228 141 286
Fragments arranged by size:
1104 1004 286 228 141 105
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1875 2634 2829 2868
Size: 1875 759 195 39
Fragments arranged by size:
1875 759 195 39
Bsp1286I G_dGCh'C
Cuts at: 0 227 352 1004 1064 1090 1097 1290 2071
Size: 227 125 652 60 26 7 193 781
Cuts at: 2071 2108 2213 2441 2582 2868
Size: 37 105 228 141 286
Fragments arranged by size:
781 652 286 228 227 193 141 125
105 60 37 26 7
BspHI T'CATG_A
Cuts at: 0 1463 2868
Size: 1463 1405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 175 1047 1311 2026 2868
Size: 175 872 264 715 842
Fragments arranged by size:
872 842 715 264 175
BsrBI CCG'CTC
Cuts at: 0 906 2160 2868
Size: 906 1254 708
Fragments arranged by size:
1254 906 708
BsrDI GCAATG_nn'
Cuts at: 0 2379 2868
Size: 2379 489
BsrFI r'CCGG_y
Cuts at: 0 2638 2868
Size: 2638 230
BstAPI GCAn_nnn'nTGC
Cuts at: 0 691 772 2457 2868
Size: 691 81 1685 411
Fragments arranged by size:
1685 691 411 81
BstEII G'GTnAC_C
Cuts at: 0 2467 2868
Size: 2467 401
BstXI CCAn_nnnn'nTGG
Cuts at: 0 217 870 1083 1426 2868
Size: 217 653 213 343 1442
Fragments arranged by size:
1442 653 343 217 213
BstYI r'GATC_y
Cuts at: 0 191 536 948 1925 2151 2273 2868
Size: 191 345 412 977 226 122 595
Fragments arranged by size:
977 595 412 345 226 191 122
Bsu36I CC'TnA_GG
Cuts at: 0 2278 2868
Size: 2278 590
BtgI C'CryG_G
Cuts at: 0 79 210 1242 1569 2463 2723 2868
Size: 79 131 1032 327 894 260 145
Fragments arranged by size:
1032 894 327 260 145 131 79
BtsI GCAGTG_nn'
Cuts at: 0 1835 1940 2802 2868
Size: 1835 105 862 66
Fragments arranged by size:
1835 862 105 66
DraIII CAC_nnn'GTG
Cuts at: 0 772 2037 2868
Size: 772 1265 831
Fragments arranged by size:
1265 831 772
DrdI GACnn_nn'nnGTC
Cuts at: 0 127 2868
Size: 127 2741
EaeI y'GGCC_r
Cuts at: 0 872 2358 2810 2868
Size: 872 1486 452 58
Fragments arranged by size:
1486 872 452 58
EarI CTCTTCn'nnn_
Cuts at: 0 1169 1853 2264 2689 2868
Size: 1169 684 411 425 179
Fragments arranged by size:
1169 684 425 411 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 793 2868
Size: 793 2075
EcoICRI GAG'CTC
Cuts at: 0 2211 2868
Size: 2211 657
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1336 1630 1753 1836 1891 1901 2227
Size: 829 507 294 123 83 55 10 326
Cuts at: 2227 2352 2633 2643 2868
Size: 125 281 10 225
Fragments arranged by size:
829 507 326 294 281 225 125 123
83 55 10 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 247 2281 2868
Size: 247 2034 587
Fragments arranged by size:
2034 587 247
EcoO109I rG'GnC_Cy
Cuts at: 0 348 349 1094 1472 1820 2654 2673 2868
Size: 348 1 745 378 348 834 19 195
Fragments arranged by size:
834 745 378 348 348 195 19 1
EcoRI G'AATT_C
Cuts at: 0 2377 2868
Size: 2377 491
FspI TGC'GCA
Cuts at: 0 2605 2868
Size: 2605 263
FspAI rTGC'GCAy
Cuts at: 0 2605 2868
Size: 2605 263
HaeII r_GCGC'y
Cuts at: 0 913 2540 2868
Size: 913 1627 328
Fragments arranged by size:
1627 913 328
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 883 915 1204 1236 1381 1413 1534 1566
Size: 883 32 289 32 145 32 121 32
Cuts at: 1566 2170 2202 2828 2860 2868
Size: 604 32 626 32 8
Fragments arranged by size:
883 626 604 289 145 121 32 32
32 32 32 32 8
HincII GTy'rAC
Cuts at: 0 405 2868
Size: 405 2463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2072 2868
Size: 2072 796
MscI TGG'CCA
Cuts at: 0 874 2868
Size: 874 1994
MslI CAynn'nnrTG
Cuts at: 0 109 1424 2086 2260 2554 2557 2713 2868
Size: 109 1315 662 174 294 3 156 155
Fragments arranged by size:
1315 662 294 174 156 155 109 3
MspA1I CmG'CkG
Cuts at: 0 174 734 1963 2199 2868
Size: 174 560 1229 236 669
Fragments arranged by size:
1229 669 560 236 174
NaeI GCC'GGC
Cuts at: 0 2640 2868
Size: 2640 228
NcoI C'CATG_G
Cuts at: 0 79 210 1569 2723 2868
Size: 79 131 1359 1154 145
Fragments arranged by size:
1359 1154 145 131 79
NgoMIV G'CCGG_C
Cuts at: 0 2638 2868
Size: 2638 230
NsiI A_TGCA'T
Cuts at: 0 1644 2868
Size: 1644 1224
NspI r_CATG'y
Cuts at: 0 397 1646 1777 2091 2868
Size: 397 1249 131 314 777
Fragments arranged by size:
1249 777 397 314 131
PasI CC'CwG_GG
Cuts at: 0 1065 2868
Size: 1065 1803
PflMI CCAn_nnn'nTGG
Cuts at: 0 775 2868
Size: 775 2093
PfoI T'CCnGG_A
Cuts at: 0 2174 2868
Size: 2174 694
PmlI CAC'GTG
Cuts at: 0 1704 2436 2868
Size: 1704 732 432
Fragments arranged by size:
1704 732 432
PpuMI rG'GwC_Cy
Cuts at: 0 2673 2868
Size: 2673 195
PspOMI G'GGCC_C
Cuts at: 0 348 1093 2868
Size: 348 745 1775
Fragments arranged by size:
1775 745 348
PspXI vC'TCGA_Gb
Cuts at: 0 1360 2868
Size: 1360 1508
PstI C_TGCA'G
Cuts at: 0 620 860 1772 2195 2868
Size: 620 240 912 423 673
Fragments arranged by size:
912 673 620 423 240
PvuI CG_AT'CG
Cuts at: 0 483 2868
Size: 483 2385
PvuII CAG'CTG
Cuts at: 0 734 1963 2868
Size: 734 1229 905
Fragments arranged by size:
1229 905 734
SacI G_AGCT'C
Cuts at: 0 2213 2868
Size: 2213 655
SexAI A'CCwGG_T
Cuts at: 0 768 2033 2868
Size: 768 1265 835
Fragments arranged by size:
1265 835 768
SfcI C'TryA_G
Cuts at: 0 616 856 1005 1248 1662 1768 2191 2868
Size: 616 240 149 243 414 106 423 677
Fragments arranged by size:
677 616 423 414 243 240 149 106
SmlI C'TyrA_G
Cuts at: 0 1279 1360 2868
Size: 1279 81 1508
Fragments arranged by size:
1508 1279 81
StuI AGG'CCT
Cuts at: 0 331 2868
Size: 331 2537
StyI C'CwwG_G
Cuts at: 0 79 155 210 278 875 1536 1569 2669
Size: 79 76 55 68 597 661 33 1100
Cuts at: 2669 2723 2868
Size: 54 145
Fragments arranged by size:
1100 661 597 145 79 76 68 55
54 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 576 1057 1060 1454 1549 2868
Size: 576 481 3 394 95 1319
Fragments arranged by size:
1319 576 481 394 95 3
TatI w'GTAC_w
Cuts at: 0 527 620 1522 2868
Size: 527 93 902 1346
Fragments arranged by size:
1346 902 527 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 909 941 983 1015 2506 2538 2868
Size: 909 32 42 32 1491 32 330
Fragments arranged by size:
1491 909 330 42 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 285 942 1567 1881 2684 2868
Size: 285 657 625 314 803 184
Fragments arranged by size:
803 657 625 314 285 184
XhoI C'TCGA_G
Cuts at: 0 1360 2868
Size: 1360 1508
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApaI ApaLI ApoI AvaI
BaeI BamHI BanI BanII BbvCI BcgI BciVI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI
PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuI
PvuII SacI SexAI SfcI SmlI StuI StyI TaqII
TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AscI AseI AsiSI AvrII BbeI
BbsI BclI BglI BmgBI BmtI BsaBI BsaWI BsiWI
BsmI BspEI BsrGI BssHII BssSI BstBI BstZ17I BtgZI
ClaI CspCI DraI EagI EcoRV FalI FseI HindIII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI PacI PciI PmeI PpiI PshAI PsiI
PsrI RsrII SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XmaI XmnI ZraI