Restriction map for
pFN21AE4227
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BcgI BciVI
BfrI BglII BlnI BsaBI BsaI
BseAI BsiEI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII EagI EclXI Eco52I Eco81I
EcoO65I EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 639 GAATTC [GA]^AATT[CT]
AflIII 1997 ACGTGT A^C[GA][CT]GT
Aor51HI 1031 AGCGCT AGC^GCT
ApoI 639 GAATTC [GA]^AATT[CT]
BbeI 848 GGCGCC GGCGC^C
BbrPI 1996 CACGTG CAC^GTG
BbvCI 2328 CCTCAGC CCTCAGC(-5/-2)
BclI 1151 TGATCA T^GATCA
BsaAI 1996 CACGTG [CT]AC^GT[GA]
BsaWI 676 ACCGGA [AT]^CCGG[AT]
BsiWI 1080 CGTACG C^GTACG
Bsp1407I 1680 TGTACA T^GTACA
BsrDI 1670 GCAATG GCAATG(2/0)
BsrGI 1680 TGTACA T^GTACA
BssSI 770 CACGAG CACGAG(-5/-1)
BstAPI 1822 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III 1031 AGCGCT AGC^GCT
EcoRI 639 GAATTC G^AATTC
EcoRV 1069 GATATC GAT^ATC
FbaI 1151 TGATCA T^GATCA
KasI 848 GGCGCC G^GCGCC
KspI 991 CCGCGG CCGC^GG
MseI 869 TTAA T^TAA
NarI 848 GGCGCC GG^CGCC
PflMI 754 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1996 CACGTG CAC^GTG
PmlI 1996 CACGTG CAC^GTG
PvuII 300 CAGCTG CAG^CTG
SacII 991 CCGCGG CCGC^GG
SapI 811 GAAGAGC GCTCTTC(1/4)
SbfI 1302 CCTGCAGG CCTGCA^GG
SfiI 1626 GGCCACCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 848 GGCGCC GGC^GCC
Sse8387I 1302 CCTGCAGG CCTGCA^GG
SspBI 1680 TGTACA T^GTACA
TfiI 860 GATTC G^A[AT]TC
Tru9I 869 TTAA T^TAA
Tsp509I 640 AATT ^AATT
Van91I 754 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2378 CCACCAGCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 1393 GTCTAC GT^[AC][GT]AC
1858 GTCTAC
AcyI 848 GGCGCC G[GA]^CG[CT]C
1093 GGCGTC
AhdI 739 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2218 GACATCCTGTC
ApaI 945 GGGCCC GGGCC^C
2054 GGGCCC
AspEI 739 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2218 GACATCCTGTC
BglI 1627 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1936 GCCAAGATGGC
BsaHI 848 GGCGCC G[GA]^CG[CT]C
1093 GGCGTC
BsmAI 855 GTCTC GTCTC(1/5)
1497 GTCTC
BstXI 33 CCACCTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2381 CCAGCAAACTGG
BstYI 1727 AGATCC [GA]^GATC[CT]
2084 GGATCT
DrdI 344 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2119 GACAAGGAGGTC
Eam1105I 739 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2218 GACATCCTGTC
HgaI 1094 GCGTC GACGC(5/10)
1512 GCGTC
Hin1I 848 GGCGCC G[GA]^CG[CT]C
1093 GGCGTC
MflI 1727 AGATCC [GA]^GATC[CT]
2084 GGATCT
MslI 549 CACCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2188 CACCCAGATG
SmlI 1564 CTCAAG C^T[CT][GA]AG
1648 CTCAAG
XhoII 1727 AGATCC [GA]^GATC[CT]
2084 GGATCT
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I XmaCI XmaI