(Circular) (Six-base) MAPSORT of: pFN21AE4227.seq Check: 8269 from: 1 to: 7111 pFN21AE4227 7111 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2029 3949 2029 Size: 1920 5191 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4921 278 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3399 3864 4454 1937 Size: 1462 465 590 4594 Fragments arranged by size: 4594 1462 590 465 Acc65I G'GTAC_C Cuts at: 4432 4432 Size: 7111 AclI AA'CG_TT Cuts at: 1563 5238 5611 1563 Size: 3675 373 3063 Fragments arranged by size: 3675 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 3334 3614 3636 3863 3998 5174 6558 Size: 512 1190 280 22 227 135 1176 1384 Cuts at: 6558 6826 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1190 1176 512 280 268 227 135 22 AfeI AGC'GCT Cuts at: 3038 3038 Size: 7111 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6914 AflIII A'CryG_T Cuts at: 1203 4002 6010 6987 1203 Size: 2799 2008 977 1327 Fragments arranged by size: 2799 2008 1327 977 AgeI A'CCGG_T Cuts at: 5942 5942 Size: 7111 AhdI GACnn_n'nnGTC Cuts at: 2749 4228 5840 2749 Size: 1479 1612 4020 Fragments arranged by size: 4020 1612 1479 AleI CACnn'nnGTG Cuts at: 1166 2558 1166 Size: 1392 5719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7111 AlwNI CAG_nnn'CTG Cuts at: 2196 2515 2765 3262 3316 3328 3451 3520 4051 Size: 319 250 497 54 12 123 69 531 Cuts at: 4051 4854 6426 2196 Size: 803 1572 2881 Fragments arranged by size: 2881 1572 803 531 497 319 250 123 69 54 12 ApaI G_GGCC'C Cuts at: 2954 4063 2954 Size: 1109 6002 ApaLI G'TGCA_C Cuts at: 5168 6324 5168 Size: 1156 5955 ApoI r'AATT_y Cuts at: 1493 2644 4420 4646 4798 6907 1493 Size: 1151 1776 226 152 2109 1697 Fragments arranged by size: 2109 1776 1697 1151 226 152 AseI AT'TA_AT Cuts at: 160 1032 5665 160 Size: 872 4633 1606 Fragments arranged by size: 4633 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 AvaI C'yCGr_G Cuts at: 1870 1943 2131 2462 2556 2655 2691 2953 3278 Size: 73 188 331 94 99 36 262 325 Cuts at: 3278 4436 1870 Size: 1158 4545 Fragments arranged by size: 4545 1158 331 325 262 188 99 94 73 36 BamHI G'GATC_C Cuts at: 4441 4808 4441 Size: 367 6744 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2324 2678 2853 3811 4300 Size: 325 217 627 537 354 175 958 489 Cuts at: 4300 4432 5887 618 Size: 132 1455 1842 Fragments arranged by size: 1842 1455 958 627 537 489 354 325 217 175 132 BanII G_rGCy'C Cuts at: 1391 2138 2185 2532 2799 2954 3614 4063 4430 Size: 747 47 347 267 155 660 449 367 Cuts at: 4430 1391 Size: 4072 Fragments arranged by size: 4072 747 660 449 367 347 267 155 47 BbeI G_GCGC'C Cuts at: 2857 2857 Size: 7111 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2470 3624 3630 4223 928 Size: 971 571 1154 6 593 3816 Fragments arranged by size: 3816 1154 971 593 571 6 BbvCI CC'TCA_GC Cuts at: 4334 4334 Size: 7111 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5302 5336 1903 Size: 34 3365 34 3678 Fragments arranged by size: 3678 3365 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5003 6213 5003 Size: 1210 5901 BclI T'GATC_A Cuts at: 1548 3156 1548 Size: 1608 5503 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3638 3947 5722 Size: 107 122 71 631 605 1966 309 1775 Cuts at: 5722 136 Size: 1525 Fragments arranged by size: 1966 1775 1525 631 605 309 122 107 71 BglII A'GATC_T Cuts at: 7106 7106 Size: 7111 BlpI GC'TnA_GC Cuts at: 3113 3227 3951 4528 3113 Size: 114 724 577 5696 Fragments arranged by size: 5696 724 577 114 Bme1580I G_kGCm'C Cuts at: 1790 2329 2662 2954 4063 4106 4305 5172 6328 Size: 539 333 292 1109 43 199 867 1156 Cuts at: 6328 1790 Size: 2573 Fragments arranged by size: 2573 1156 1109 867 539 333 292 199 43 BmgBI CAC'GTC Cuts at: 1318 2905 3364 1318 Size: 1587 459 5065 Fragments arranged by size: 5065 1587 459 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2055 2132 4403 Size: 416 53 83 399 24 638 77 2271 Cuts at: 4403 4842 5800 442 Size: 439 958 1753 Fragments arranged by size: 2271 1753 958 638 439 416 399 83 77 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7111 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2582 2582 Size: 7111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2015 2363 2374 3338 3350 3479 3515 3998 Size: 776 348 11 964 12 129 36 483 Cuts at: 3998 4093 5771 1239 Size: 95 1678 2579 Fragments arranged by size: 2579 1678 964 776 483 348 129 95 36 12 11 Bpu10I CC'TnA_GC Cuts at: 2516 2577 3722 3971 4334 2516 Size: 61 1145 249 363 5293 Fragments arranged by size: 5293 1145 363 249 61 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3554 3638 5236 6101 6399 3554 Size: 84 1598 865 298 4266 Fragments arranged by size: 4266 1598 865 298 84 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5774 882 Size: 617 4275 2219 Fragments arranged by size: 4275 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4003 4902 493 Size: 711 2799 899 2702 Fragments arranged by size: 2799 2702 899 711 BsaBI GATnn'nnATC Cuts at: 7105 7105 Size: 7111 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2854 3099 4918 5300 Size: 53 83 186 936 1321 245 1819 382 Cuts at: 5300 5935 275 Size: 635 1451 Fragments arranged by size: 1819 1451 1321 936 635 382 245 186 83 53 BsaWI w'CCGG_w Cuts at: 2681 5544 5942 6216 6363 6774 2681 Size: 2863 398 274 147 411 3018 Fragments arranged by size: 3018 2863 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2084 2114 2393 2423 3131 Size: 30 165 30 681 30 279 30 708 Cuts at: 3131 3161 3786 3816 1178 Size: 30 625 30 4473 Fragments arranged by size: 4473 708 681 625 279 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2540 2957 2960 2993 3512 4001 4010 Size: 1000 354 417 3 33 519 489 9 Cuts at: 4010 1186 Size: 4287 Fragments arranged by size: 4287 1000 519 489 417 354 33 9 3 BseYI C'CCAG_C Cuts at: 2291 2328 2335 3014 6314 6810 2291 Size: 37 7 679 3300 496 2592 Fragments arranged by size: 3300 2592 679 496 37 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2603 3686 4175 1163 Size: 573 867 1083 489 4099 Fragments arranged by size: 4099 1083 867 573 489 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4561 5322 6350 1540 Size: 275 186 2560 761 1028 2301 Fragments arranged by size: 2560 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2522 2843 3293 5172 5257 6328 2522 Size: 321 450 1879 85 1071 3305 Fragments arranged by size: 3305 1879 1071 450 321 85 BsiWI C'GTAC_G Cuts at: 3085 3085 Size: 7111 BsmI GAATG_Cn' Cuts at: 4622 4715 4622 Size: 93 7018 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2185 2329 2522 2532 2662 2799 Size: 399 348 47 144 193 10 130 137 Cuts at: 2799 2843 2954 3293 3614 4063 4106 4305 4430 Size: 44 111 339 321 449 43 199 125 Cuts at: 4430 5172 5257 6328 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 449 399 348 339 321 199 193 144 137 130 125 111 85 47 44 43 10 BspEI T'CCGG_A Cuts at: 6774 6774 Size: 7111 BspHI T'CATG_A Cuts at: 5000 6730 5000 Size: 1730 5381 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2029 3411 3949 4466 844 Size: 1185 1382 538 517 3489 Fragments arranged by size: 3489 1382 1185 538 517 BsrBI CCG'CTC Cuts at: 1394 1823 2581 2739 2851 4998 1394 Size: 429 758 158 112 2147 3507 Fragments arranged by size: 3507 2147 758 429 158 112 BsrDI GCAATG_nn' Cuts at: 65 1248 3682 5606 5780 65 Size: 1183 2434 1924 174 1396 Fragments arranged by size: 2434 1924 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5755 5942 1700 Size: 110 3945 187 2869 Fragments arranged by size: 3945 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3685 96 Size: 3589 3522 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7111 BssSI C'ACGA_G Cuts at: 2775 5171 6183 2775 Size: 2396 1012 3703 Fragments arranged by size: 3703 2396 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3833 794 Size: 3039 4072 BstBI TT'CG_AA Cuts at: 5915 5915 Size: 7111 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7111 BstXI CCAn_nnnn'nTGG Cuts at: 2045 4393 2045 Size: 2348 4763 BstYI r'GATC_y Cuts at: 1972 3732 4089 4441 4808 5193 5210 6662 6748 Size: 1760 357 352 367 385 17 1452 86 Cuts at: 6748 6760 7106 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1760 1452 385 367 357 352 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2392 2996 4024 513 Size: 552 939 388 604 1028 3600 Fragments arranged by size: 3600 1028 939 604 552 388 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4099 487 Size: 683 56 2873 3499 Fragments arranged by size: 3499 2873 683 56 BtsI GCAGTG_nn' Cuts at: 2058 2085 3263 3376 3815 4187 4627 5421 5441 Size: 27 1178 113 439 372 440 794 20 Cuts at: 5441 2058 Size: 3728 Fragments arranged by size: 3728 1178 794 440 439 372 113 27 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7111 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7076 DraI TTT'AAA Cuts at: 4415 4415 Size: 7111 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2355 4130 6118 809 Size: 507 1039 1775 1988 1802 Fragments arranged by size: 1988 1802 1775 1039 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2237 2309 2491 Size: 54 1425 50 275 18 407 72 182 Cuts at: 2491 2756 2907 3105 3630 3997 4558 5447 7076 Size: 265 151 198 525 367 561 889 1629 Cuts at: 7076 8 Size: 43 Fragments arranged by size: 1629 1425 889 561 525 407 367 275 265 198 182 151 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4558 1537 Size: 275 2746 4090 Fragments arranged by size: 4090 2746 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2439 2629 2811 3692 3881 4314 5041 1353 Size: 1086 190 182 881 189 433 727 3423 Fragments arranged by size: 3423 1086 881 727 433 190 189 182 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2656 3919 4262 5698 6072 6218 Size: 1064 138 1067 1263 343 1436 374 146 Cuts at: 6218 387 Size: 1280 Fragments arranged by size: 1436 1280 1263 1067 1064 374 343 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2015 2144 2363 2374 3334 3338 3350 Size: 393 383 129 219 11 960 4 12 Cuts at: 3350 3479 3515 3614 3636 3863 3998 4093 5174 Size: 129 36 99 22 227 135 95 1081 Cuts at: 5174 5771 6558 6826 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1081 960 787 597 393 383 268 227 219 135 129 129 99 95 36 22 12 11 4 EcoNI CCTnn'n_nnAGG Cuts at: 2223 2888 3553 2223 Size: 665 665 5781 Fragments arranged by size: 5781 665 665 EcoO109I rG'GnC_Cy Cuts at: 2208 2348 2449 2512 2573 2950 3313 3967 4239 Size: 140 101 63 61 377 363 654 272 Cuts at: 4239 2208 Size: 5080 Fragments arranged by size: 5080 654 377 363 272 140 101 63 61 EcoRI G'AATT_C Cuts at: 2644 4420 4798 2644 Size: 1776 378 4957 Fragments arranged by size: 4957 1776 378 EcoRV GAT'ATC Cuts at: 3076 3076 Size: 7111 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3754 3754 Size: 7111 FspI TGC'GCA Cuts at: 5617 5617 Size: 7111 HaeII r_GCGC'y Cuts at: 1678 2383 2590 2857 2916 3040 3451 4341 6258 Size: 705 207 267 59 124 411 890 1917 Cuts at: 6258 1678 Size: 2531 Fragments arranged by size: 2531 1917 890 705 411 267 207 124 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 2393 2425 2735 Size: 32 135 32 512 32 277 32 310 Cuts at: 2735 2767 3220 3252 4214 4246 5752 5784 5826 Size: 32 453 32 962 32 1506 32 42 Cuts at: 5826 5858 1373 Size: 32 2626 Fragments arranged by size: 2626 1506 962 512 453 310 277 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4455 4701 669 Size: 988 281 2517 246 3079 Fragments arranged by size: 3079 2517 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7111 HpaI GTT'AAC Cuts at: 4701 4701 Size: 7111 KasI G'GCGC_C Cuts at: 2853 2853 Size: 7111 KpnI G_GTAC'C Cuts at: 4436 4436 Size: 7111 MfeI C'AATT_G Cuts at: 4710 4710 Size: 7111 MluI A'CGCG_T Cuts at: 6987 6987 Size: 7111 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6225 6409 1305 Size: 4920 184 2007 Fragments arranged by size: 4920 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2239 2311 2493 2909 3107 Size: 54 1425 343 407 72 182 416 198 Cuts at: 3107 3632 10 Size: 525 3489 Fragments arranged by size: 3489 1425 525 416 407 343 198 182 72 54 MslI CAynn'nnrTG Cuts at: 518 1166 2558 4197 5069 5428 5587 518 Size: 648 1392 1639 872 359 159 2042 Fragments arranged by size: 2042 1639 1392 872 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2196 2277 2307 2998 3096 3325 3760 3777 Size: 590 81 30 691 98 229 435 17 Cuts at: 3777 4527 5204 6352 6597 1606 Size: 750 677 1148 245 2120 Fragments arranged by size: 2120 1148 750 691 677 590 435 245 229 98 81 30 17 NarI GG'CG_CC Cuts at: 2854 2854 Size: 7111 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5620 Fragments arranged by size: 5620 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7111 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7111 NotI GC'GGCC_GC Cuts at: 4558 4558 Size: 7111 NspI r_CATG'y Cuts at: 1132 1229 4469 6014 1132 Size: 97 3240 1545 2229 Fragments arranged by size: 3240 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2203 2532 1348 Size: 855 329 5927 Fragments arranged by size: 5927 855 329 PciI A'CATG_T Cuts at: 6010 6010 Size: 7111 PflMI CCAn_nnn'nTGG Cuts at: 1108 2765 1108 Size: 1657 5454 PfoI T'CCnGG_A Cuts at: 2834 3456 4356 2834 Size: 622 900 5589 Fragments arranged by size: 5589 900 622 PmeI GTTT'AAAC Cuts at: 4415 4415 Size: 7111 PmlI CAC'GTG Cuts at: 4003 4003 Size: 7111 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5180 5212 6693 6725 5180 Size: 32 1481 32 5566 Fragments arranged by size: 5566 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2208 2348 2449 2573 3313 3967 4239 2208 Size: 140 101 124 740 654 272 5080 Fragments arranged by size: 5080 740 654 272 140 124 101 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7111 PsiI TTA'TAA Cuts at: 4681 4681 Size: 7111 PspOMI G'GGCC_C Cuts at: 2950 4059 2950 Size: 1109 6002 PstI C_TGCA'G Cuts at: 830 3138 3312 3826 3960 3987 4463 830 Size: 2308 174 514 134 27 476 3478 Fragments arranged by size: 3478 2308 514 476 174 134 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7111 PvuII CAG'CTG Cuts at: 2307 2307 Size: 7111 SacII CC_GC'GG Cuts at: 2999 2999 Size: 7111 SalI G'TCGA_C Cuts at: 1936 4453 1936 Size: 2517 4594 SanDI GG'GwC_CC Cuts at: 2208 2348 2208 Size: 140 6971 SapI GCTCTTCn'nnn_ Cuts at: 2811 2811 Size: 7111 SbfI CC_TGCA'GG Cuts at: 3312 4463 3312 Size: 1151 5960 ScaI AGT'ACT Cuts at: 5359 7071 5359 Size: 1712 5399 SfcI C'TryA_G Cuts at: 826 1045 2073 3134 3308 3822 3956 3983 4459 Size: 219 1028 1061 174 514 134 27 476 Cuts at: 4459 5594 6275 6466 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1061 1028 681 514 476 219 191 174 134 27 SfiI GGCCn_nnn'nGGCC Cuts at: 3638 3638 Size: 7111 SfoI GGC'GCC Cuts at: 2855 2855 Size: 7111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7111 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7111 SmaI CCC'GGG Cuts at: 1872 2657 2693 2955 4438 1872 Size: 785 36 262 1483 4545 Fragments arranged by size: 4545 1483 785 262 36 SmlI C'TyrA_G Cuts at: 820 1017 1943 3569 3653 5215 6116 6378 820 Size: 197 926 1626 84 1562 901 262 1553 Fragments arranged by size: 1626 1562 1553 926 901 262 197 84 SnaBI TAC'GTA Cuts at: 493 4902 493 Size: 4409 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7111 SphI G_CATG'C Cuts at: 1132 4469 1132 Size: 3337 3774 SrfI GCCC'GGGC Cuts at: 2657 2657 Size: 7111 SspI AAT'ATT Cuts at: 5 52 5035 5 Size: 47 4983 2081 Fragments arranged by size: 4983 2081 47 StuI AGG'CCT Cuts at: 1517 3170 3425 3641 4310 1517 Size: 1653 255 216 669 4318 Fragments arranged by size: 4318 1653 669 255 216 StyI C'CwwG_G Cuts at: 513 1065 2004 2224 3636 4209 4550 513 Size: 552 939 220 1412 573 341 3074 Fragments arranged by size: 3074 1412 939 573 552 341 220 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4209 4288 5138 5155 5308 5493 5938 Size: 1255 2629 79 850 17 153 185 445 Cuts at: 5938 325 Size: 1498 Fragments arranged by size: 2629 1498 1255 850 445 185 153 79 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3685 5357 7069 Size: 274 80 33 51 1443 1708 1672 1712 Cuts at: 7069 96 Size: 138 Fragments arranged by size: 1712 1708 1672 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3242 3274 4057 4089 7071 7103 3242 Size: 32 783 32 2982 32 3250 Fragments arranged by size: 3250 2982 783 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7111 XbaI T'CTAG_A Cuts at: 4447 4447 Size: 7111 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4390 4390 Size: 7111 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7111 XmaI C'CCGG_G Cuts at: 1870 2655 2691 2953 4436 1870 Size: 785 36 262 1483 4545 Fragments arranged by size: 4545 1483 785 262 36 XmnI GAAnn'nnTTC Cuts at: 5240 5240 Size: 7111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4919 276 Size: 53 83 186 936 3385 2468 Fragments arranged by size: 3385 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SexAI SwaI