Restriction map for
pFN21AE4227
(Linear) (Six-base) MAPSORT of: KIEE4227.seq Check: 2450 from: 1 to: 2406
KIEE4227.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 24 1944 2406
Size: 24 1920 462
Fragments arranged by size:
1920 462 24
AccI GT'mk_AC
Cuts at: 0 1394 1859 2406
Size: 1394 465 547
Fragments arranged by size:
1394 547 465
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1329 1609 1631 1858 1993 2406
Size: 139 1190 280 22 227 135 413
Fragments arranged by size:
1190 413 280 227 139 135 22
AfeI AGC'GCT
Cuts at: 0 1033 2406
Size: 1033 1373
AflIII A'CryG_T
Cuts at: 0 1997 2406
Size: 1997 409
AhdI GACnn_n'nnGTC
Cuts at: 0 744 2223 2406
Size: 744 1479 183
Fragments arranged by size:
1479 744 183
AleI CACnn'nnGTG
Cuts at: 0 553 2406
Size: 553 1853
AlwNI CAG_nnn'CTG
Cuts at: 0 191 510 760 1257 1311 1323 1446 1515
Size: 191 319 250 497 54 12 123 69
Cuts at: 1515 2046 2406
Size: 531 360
Fragments arranged by size:
531 497 360 319 250 191 123 69
54 12
ApaI G_GGCC'C
Cuts at: 0 949 2058 2406
Size: 949 1109 348
Fragments arranged by size:
1109 949 348
ApoI r'AATT_y
Cuts at: 0 639 2406
Size: 639 1767
AvaI C'yCGr_G
Cuts at: 0 126 457 551 650 686 948 1273 2406
Size: 126 331 94 99 36 262 325 1133
Fragments arranged by size:
1133 331 325 262 126 99 94 36
BanI G'GyrC_C
Cuts at: 0 319 673 848 1806 2295 2406
Size: 319 354 175 958 489 111
Fragments arranged by size:
958 489 354 319 175 111
BanII G_rGCy'C
Cuts at: 0 133 180 527 794 949 1609 2058 2406
Size: 133 47 347 267 155 660 449 348
Fragments arranged by size:
660 449 348 347 267 155 133 47
BbeI G_GCGC'C
Cuts at: 0 852 2406
Size: 852 1554
BbsI GAAGACnn'nnnn_
Cuts at: 0 465 1619 1625 2218 2406
Size: 465 1154 6 593 188
Fragments arranged by size:
1154 593 465 188 6
BbvCI CC'TCA_GC
Cuts at: 0 2329 2406
Size: 2329 77
BclI T'GATC_A
Cuts at: 0 1151 2406
Size: 1151 1255
BglI GCCn_nnn'nGGC
Cuts at: 0 1633 1942 2406
Size: 1633 309 464
Fragments arranged by size:
1633 464 309
BlpI GC'TnA_GC
Cuts at: 0 1108 1222 1946 2406
Size: 1108 114 724 460
Fragments arranged by size:
1108 724 460 114
Bme1580I G_kGCm'C
Cuts at: 0 324 657 949 2058 2101 2300 2406
Size: 324 333 292 1109 43 199 106
Fragments arranged by size:
1109 333 324 292 199 106 43
BmgBI CAC'GTC
Cuts at: 0 900 1359 2406
Size: 900 459 1047
Fragments arranged by size:
1047 900 459
BmrI ACTGGGnnnn_n'
Cuts at: 0 50 127 2398 2406
Size: 50 77 2271 8
Fragments arranged by size:
2271 77 50 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 577 2406
Size: 577 1829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 358 369 1333 1345 1474 1510 1993
Size: 10 348 11 964 12 129 36 483
Cuts at: 1993 2088 2406
Size: 95 318
Fragments arranged by size:
964 483 348 318 129 95 36 12
11 10
Bpu10I CC'TnA_GC
Cuts at: 0 511 572 1717 1966 2329 2406
Size: 511 61 1145 249 363 77
Fragments arranged by size:
1145 511 363 249 77 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1549 1633 2406
Size: 1549 84 773
Fragments arranged by size:
1549 773 84
BsaAI yAC'GTr
Cuts at: 0 1998 2406
Size: 1998 408
BsaHI Gr'CG_yC
Cuts at: 0 849 1094 2406
Size: 849 245 1312
Fragments arranged by size:
1312 849 245
BsaWI w'CCGG_w
Cuts at: 0 676 2406
Size: 676 1730
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 388 418 1126 1156 1781 1811
Size: 79 30 279 30 708 30 625 30
Cuts at: 1811 2406
Size: 595
Fragments arranged by size:
708 625 595 279 79 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 535 952 955 988 1507 1996 2005
Size: 181 354 417 3 33 519 489 9
Cuts at: 2005 2406
Size: 401
Fragments arranged by size:
519 489 417 401 354 181 33 9
3
BseYI C'CCAG_C
Cuts at: 0 286 323 330 1009 2406
Size: 286 37 7 679 1397
Fragments arranged by size:
1397 679 286 37 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 1681 2170 2406
Size: 598 1083 489 236
Fragments arranged by size:
1083 598 489 236
BsiHKAI G_wGCw'C
Cuts at: 0 517 838 1288 2406
Size: 517 321 450 1118
Fragments arranged by size:
1118 517 450 321
BsiWI C'GTAC_G
Cuts at: 0 1080 2406
Size: 1080 1326
Bsp1286I G_dGCh'C
Cuts at: 0 133 180 324 517 527 657 794 838
Size: 133 47 144 193 10 130 137 44
Cuts at: 838 949 1288 1609 2058 2101 2300 2406
Size: 111 339 321 449 43 199 106
Fragments arranged by size:
449 339 321 199 193 144 137 133
130 111 106 47 44 43 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 24 1406 1944 2406
Size: 24 1382 538 462
Fragments arranged by size:
1382 538 462 24
BsrBI CCG'CTC
Cuts at: 0 576 734 846 2406
Size: 576 158 112 1560
Fragments arranged by size:
1560 576 158 112
BsrDI GCAATG_nn'
Cuts at: 0 1677 2406
Size: 1677 729
BsrGI T'GTAC_A
Cuts at: 0 1680 2406
Size: 1680 726
BssSI C'ACGA_G
Cuts at: 0 770 2406
Size: 770 1636
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1828 2406
Size: 1828 578
BstXI CCAn_nnnn'nTGG
Cuts at: 0 40 2388 2406
Size: 40 2348 18
Fragments arranged by size:
2348 40 18
BstYI r'GATC_y
Cuts at: 0 1727 2084 2406
Size: 1727 357 322
Fragments arranged by size:
1727 357 322
BtgI C'CryG_G
Cuts at: 0 387 991 2019 2406
Size: 387 604 1028 387
Fragments arranged by size:
1028 604 387 387
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2094 2406
Size: 2094 312
BtsI GCAGTG_nn'
Cuts at: 0 53 80 1258 1371 1810 2182 2406
Size: 53 27 1178 113 439 372 224
Fragments arranged by size:
1178 439 372 224 113 53 27
DrdI GACnn_nn'nnGTC
Cuts at: 0 350 2125 2406
Size: 350 1775 281
Fragments arranged by size:
1775 350 281
EaeI y'GGCC_r
Cuts at: 0 232 304 486 751 902 1100 1625 1992
Size: 232 72 182 265 151 198 525 367
Cuts at: 1992 2406
Size: 414
Fragments arranged by size:
525 414 367 265 232 198 182 151
72
EarI CTCTTCn'nnn_
Cuts at: 0 434 624 806 1687 1876 2309 2406
Size: 434 190 182 881 189 433 97
Fragments arranged by size:
881 434 433 190 189 182 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 651 1914 2257 2406
Size: 651 1263 343 149
Fragments arranged by size:
1263 651 343 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 139 358 369 1329 1333 1345 1474
Size: 10 129 219 11 960 4 12 129
Cuts at: 1474 1510 1609 1631 1858 1993 2088 2406
Size: 36 99 22 227 135 95 318
Fragments arranged by size:
960 318 227 219 135 129 129 99
95 36 22 12 11 10 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 218 883 1548 2406
Size: 218 665 665 858
Fragments arranged by size:
858 665 665 218
EcoO109I rG'GnC_Cy
Cuts at: 0 203 343 444 507 568 945 1308 1962
Size: 203 140 101 63 61 377 363 654
Cuts at: 1962 2234 2406
Size: 272 172
Fragments arranged by size:
654 377 363 272 203 172 140 101
63 61
EcoRI G'AATT_C
Cuts at: 0 639 2406
Size: 639 1767
EcoRV GAT'ATC
Cuts at: 0 1071 2406
Size: 1071 1335
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1749 2406
Size: 1749 657
HaeII r_GCGC'y
Cuts at: 0 378 585 852 911 1035 1446 2336 2406
Size: 378 207 267 59 124 411 890 70
Fragments arranged by size:
890 411 378 267 207 124 70 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 388 420 730 762 1215 1247
Size: 79 32 277 32 310 32 453 32
Cuts at: 1247 2209 2241 2406
Size: 962 32 165
Fragments arranged by size:
962 453 310 277 165 79 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 848 2406
Size: 848 1558
MscI TGG'CCA
Cuts at: 0 234 306 488 904 1102 1627 2406
Size: 234 72 182 416 198 525 779
Fragments arranged by size:
779 525 416 234 198 182 72
MslI CAynn'nnrTG
Cuts at: 0 553 2192 2406
Size: 553 1639 214
Fragments arranged by size:
1639 553 214
MspA1I CmG'CkG
Cuts at: 0 191 272 302 993 1091 1320 1755 1772
Size: 191 81 30 691 98 229 435 17
Cuts at: 1772 2406
Size: 634
Fragments arranged by size:
691 634 435 229 191 98 81 30
17
NarI GG'CG_CC
Cuts at: 0 849 2406
Size: 849 1557
PasI CC'CwG_GG
Cuts at: 0 198 527 2406
Size: 198 329 1879
Fragments arranged by size:
1879 329 198
PflMI CCAn_nnn'nTGG
Cuts at: 0 760 2406
Size: 760 1646
PfoI T'CCnGG_A
Cuts at: 0 829 1451 2351 2406
Size: 829 622 900 55
Fragments arranged by size:
900 829 622 55
PmlI CAC'GTG
Cuts at: 0 1998 2406
Size: 1998 408
PpuMI rG'GwC_Cy
Cuts at: 0 203 343 444 568 1308 1962 2234 2406
Size: 203 140 101 124 740 654 272 172
Fragments arranged by size:
740 654 272 203 172 140 124 101
PspOMI G'GGCC_C
Cuts at: 0 945 2054 2406
Size: 945 1109 352
Fragments arranged by size:
1109 945 352
PstI C_TGCA'G
Cuts at: 0 1133 1307 1821 1955 1982 2406
Size: 1133 174 514 134 27 424
Fragments arranged by size:
1133 514 424 174 134 27
PvuII CAG'CTG
Cuts at: 0 302 2406
Size: 302 2104
SacII CC_GC'GG
Cuts at: 0 994 2406
Size: 994 1412
SanDI GG'GwC_CC
Cuts at: 0 203 343 2406
Size: 203 140 2063
Fragments arranged by size:
2063 203 140
SapI GCTCTTCn'nnn_
Cuts at: 0 806 2406
Size: 806 1600
SbfI CC_TGCA'GG
Cuts at: 0 1307 2406
Size: 1307 1099
SfcI C'TryA_G
Cuts at: 0 68 1129 1303 1817 1951 1978 2406
Size: 68 1061 174 514 134 27 428
Fragments arranged by size:
1061 514 428 174 134 68 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1633 2406
Size: 1633 773
SfoI GGC'GCC
Cuts at: 0 850 2406
Size: 850 1556
SmaI CCC'GGG
Cuts at: 0 652 688 950 2406
Size: 652 36 262 1456
Fragments arranged by size:
1456 652 262 36
SmlI C'TyrA_G
Cuts at: 0 1564 1648 2406
Size: 1564 84 758
Fragments arranged by size:
1564 758 84
SrfI GCCC'GGGC
Cuts at: 0 652 2406
Size: 652 1754
StuI AGG'CCT
Cuts at: 0 1165 1420 1636 2305 2406
Size: 1165 255 216 669 101
Fragments arranged by size:
1165 669 255 216 101
StyI C'CwwG_G
Cuts at: 0 219 1631 2204 2406
Size: 219 1412 573 202
Fragments arranged by size:
1412 573 219 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2204 2283 2406
Size: 2204 79 123
Fragments arranged by size:
2204 123 79
TatI w'GTAC_w
Cuts at: 0 1680 2406
Size: 1680 726
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1237 1269 2052 2084 2406
Size: 1237 32 783 32 322
Fragments arranged by size:
1237 783 322 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2385 2406
Size: 2385 21
XmaI C'CCGG_G
Cuts at: 0 650 686 948 2406
Size: 650 36 262 1458
Fragments arranged by size:
1458 650 262 36
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsiWI Bsp1286I BspMI BsrBI BsrDI BsrGI
BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DrdI
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I KasI MscI MslI MspA1I NarI
PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacII SanDI SapI SbfI SfcI SfiI SfoI SmaI
SmlI SrfI StuI StyI TaqII TatI TstI XcmI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AlfI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BfrBI BglII BmtI BsaI BsaBI BsiEI BsmI BsmBI
BspEI BspHI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII EagI EcoICRI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI ScaI SexAI SgfI
SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI
XhoI XmnI ZraI