(Circular) (Six-base) MAPSORT of: pFN21AA0292.seq Check: 4574 from: 1 to: 10585 pFN21AA0292 10585 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8395 278 Size: 53 83 186 936 6859 2468 Fragments arranged by size: 6859 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2996 7928 1937 Size: 1059 4932 4594 Fragments arranged by size: 4932 4594 1059 Acc65I G'GTAC_C Cuts at: 3331 7906 3331 Size: 4575 6010 AclI AA'CG_TT Cuts at: 1563 8712 9085 1563 Size: 7149 373 3063 Fragments arranged by size: 7149 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3079 3528 3546 3557 4259 5859 6204 6288 Size: 1447 449 18 11 702 1600 345 84 Cuts at: 6288 6558 7482 8648 10032 10300 1632 Size: 270 924 1166 1384 268 1917 Fragments arranged by size: 1917 1600 1447 1384 1166 924 702 449 345 270 268 84 18 11 AfeI AGC'GCT Cuts at: 7638 7638 Size: 10585 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10388 AflIII A'CryG_T Cuts at: 1203 3420 9484 10461 1203 Size: 2217 6064 977 1327 Fragments arranged by size: 6064 2217 1327 977 AgeI A'CCGG_T Cuts at: 9416 9416 Size: 10585 AhdI GACnn_n'nnGTC Cuts at: 4631 9314 4631 Size: 4683 5902 AleI CACnn'nnGTG Cuts at: 1166 4668 7266 1166 Size: 3502 2598 4485 Fragments arranged by size: 4485 3502 2598 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3511 7284 1184 Size: 2327 3773 4485 Fragments arranged by size: 4485 3773 2327 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5296 5328 5296 Size: 32 10553 AlwNI CAG_nnn'CTG Cuts at: 2195 2198 2584 3356 4490 6368 6865 7442 8328 Size: 3 386 772 1134 1878 497 577 886 Cuts at: 8328 9900 2195 Size: 1572 2880 Fragments arranged by size: 2880 1878 1572 1134 886 772 577 497 386 3 ApaI G_GGCC'C Cuts at: 4073 4460 6778 4073 Size: 387 2318 7880 Fragments arranged by size: 7880 2318 387 ApaLI G'TGCA_C Cuts at: 8642 9798 8642 Size: 1156 9429 ApoI r'AATT_y Cuts at: 1493 2122 2899 3234 3502 4247 5242 7556 7894 Size: 629 777 335 268 745 995 2314 338 Cuts at: 7894 8120 8272 10381 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2314 2109 1697 995 777 745 629 338 335 268 226 152 AseI AT'TA_AT Cuts at: 160 1032 9139 160 Size: 872 8107 1606 Fragments arranged by size: 8107 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10585 AvaI C'yCGr_G Cuts at: 1870 1943 3831 5205 7910 1870 Size: 73 1888 1374 2705 4545 Fragments arranged by size: 4545 2705 1888 1374 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5255 5288 5255 Size: 33 10552 BamHI G'GATC_C Cuts at: 7915 8282 7915 Size: 367 10218 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3331 6389 7107 7368 7685 Size: 325 217 627 1544 3058 718 261 317 Cuts at: 7685 7788 7906 9361 618 Size: 103 118 1455 1842 Fragments arranged by size: 3058 1842 1544 1455 718 627 325 317 261 217 118 103 BanII G_rGCy'C Cuts at: 1391 4073 4460 4651 5212 5408 6778 7422 7904 Size: 2682 387 191 561 196 1370 644 482 Cuts at: 7904 1391 Size: 4072 Fragments arranged by size: 4072 2682 1370 644 561 482 387 196 191 BbeI G_GCGC'C Cuts at: 7372 7689 7372 Size: 317 10268 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2706 3461 3815 4803 6294 6347 7086 Size: 971 807 755 354 988 1491 53 739 Cuts at: 7086 7389 928 Size: 303 4124 Fragments arranged by size: 4124 1491 988 971 807 755 739 354 303 53 BbvCI CC'TCA_GC Cuts at: 2325 5676 6687 6960 7859 2325 Size: 3351 1011 273 899 5051 Fragments arranged by size: 5051 3351 1011 899 273 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 1997 2021 2031 8776 8810 1903 Size: 34 50 10 24 10 6745 34 3678 Fragments arranged by size: 6745 3678 50 34 34 24 10 10 BciVI GTATCCnnnnn_n' Cuts at: 4194 8477 9687 4194 Size: 4283 1210 5092 Fragments arranged by size: 5092 4283 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 10585 BfrBI ATG'CAT Cuts at: 3953 3953 Size: 10585 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2341 4053 4969 Size: 107 122 71 631 605 669 1712 916 Cuts at: 4969 5480 6521 9196 136 Size: 511 1041 2675 1525 Fragments arranged by size: 2675 1712 1525 1041 916 669 631 605 511 122 107 71 BglII A'GATC_T Cuts at: 10580 10580 Size: 10585 BlpI GC'TnA_GC Cuts at: 8002 8002 Size: 10585 Bme1580I G_kGCm'C Cuts at: 1790 4073 4460 5476 6778 7110 7491 7793 7820 Size: 2283 387 1016 1302 332 381 302 27 Cuts at: 7820 8646 9802 1790 Size: 826 1156 2573 Fragments arranged by size: 2573 2283 1302 1156 1016 826 387 381 332 302 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10585 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2484 3189 3276 Size: 416 53 83 399 24 1067 705 87 Cuts at: 3276 4462 4667 5463 5793 8316 9274 442 Size: 1186 205 796 330 2523 958 1753 Fragments arranged by size: 2523 1753 1186 1067 958 796 705 416 399 330 205 87 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2217 4404 1056 Size: 1161 2187 7237 Fragments arranged by size: 7237 2187 1161 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3766 6195 7795 3766 Size: 2429 1600 6556 Fragments arranged by size: 6556 2429 1600 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2283 2472 3102 3176 3559 3960 4866 4926 Size: 1044 189 630 74 383 401 906 60 Cuts at: 4926 5323 5577 5598 6483 7538 9245 1239 Size: 397 254 21 885 1055 1707 2579 Fragments arranged by size: 2579 1707 1055 1044 906 885 630 401 397 383 254 189 74 60 21 Bpu10I CC'TnA_GC Cuts at: 2325 3762 5319 5676 6687 6960 7512 7794 7859 Size: 1437 1557 357 1011 273 552 282 65 Cuts at: 7859 2325 Size: 5051 Fragments arranged by size: 5051 1557 1437 1011 552 357 282 273 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3372 4305 4803 8710 9575 9873 3372 Size: 933 498 3907 865 298 4084 Fragments arranged by size: 4084 3907 933 865 498 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2213 4437 4842 5082 6430 6622 9248 Size: 617 714 2224 405 240 1348 192 2626 Cuts at: 9248 882 Size: 2219 Fragments arranged by size: 2626 2224 2219 1348 714 617 405 240 192 BsaAI yAC'GTr Cuts at: 493 1204 6280 8376 493 Size: 711 5076 2096 2702 Fragments arranged by size: 5076 2702 2096 711 BsaBI GATnn'nnATC Cuts at: 10579 10579 Size: 10585 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7369 7686 8392 8774 Size: 53 83 186 936 5836 317 706 382 Cuts at: 8774 9409 275 Size: 635 1451 Fragments arranged by size: 5836 1451 936 706 635 382 317 186 83 53 BsaWI w'CCGG_w Cuts at: 5136 7146 9018 9416 9690 9837 10248 5136 Size: 2010 1872 398 274 147 411 5473 Fragments arranged by size: 5473 2010 1872 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2389 2419 3148 3178 5296 Size: 30 165 30 986 30 729 30 2118 Cuts at: 5296 5326 6126 6156 1178 Size: 30 800 30 5607 Fragments arranged by size: 5607 2118 986 800 729 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2199 2313 2716 2719 2722 2725 2728 3156 Size: 1013 114 403 3 3 3 3 428 Cuts at: 3156 3435 3505 3667 3996 3999 5303 5779 6115 Size: 279 70 162 329 3 1304 476 336 Cuts at: 6115 7313 7316 7784 1186 Size: 1198 3 468 3987 Fragments arranged by size: 3987 1304 1198 1013 476 468 428 403 336 329 279 162 114 70 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3963 5483 6128 6777 6858 9788 10284 3963 Size: 1520 645 649 81 2930 496 4264 Fragments arranged by size: 4264 2930 1520 649 645 496 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 3481 1163 Size: 573 633 1112 8267 Fragments arranged by size: 8267 1112 633 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8035 8796 9824 1540 Size: 275 186 6034 761 1028 2301 Fragments arranged by size: 6034 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2500 3379 3629 3697 8646 8731 9802 2500 Size: 879 250 68 4949 85 1071 3283 Fragments arranged by size: 4949 3283 1071 879 250 85 68 BsmI GAATG_Cn' Cuts at: 2809 5888 8096 8189 2809 Size: 3079 2208 93 5205 Fragments arranged by size: 5205 3079 2208 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4706 4706 Size: 10585 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2500 3379 3629 3697 4073 4460 4651 Size: 399 710 879 250 68 376 387 191 Cuts at: 4651 5212 5408 5476 6778 7110 7422 7491 7793 Size: 561 196 68 1302 332 312 69 302 Cuts at: 7793 7820 7904 8646 8731 9802 1391 Size: 27 84 742 85 1071 2174 Fragments arranged by size: 2174 1302 1071 879 742 710 561 399 387 376 332 312 302 250 196 191 85 84 69 68 68 27 BspEI T'CCGG_A Cuts at: 10248 10248 Size: 10585 BspHI T'CATG_A Cuts at: 5032 5760 8474 10204 5032 Size: 728 2714 1730 5413 Fragments arranged by size: 5413 2714 1730 728 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5225 7940 844 Size: 4381 2715 3489 Fragments arranged by size: 4381 3489 2715 BsrBI CCG'CTC Cuts at: 1394 1823 4779 7377 7879 8472 1394 Size: 429 2956 2598 502 593 3507 Fragments arranged by size: 3507 2956 2598 593 502 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3931 4900 7558 9080 9254 65 Size: 1183 2683 969 2658 1522 174 1396 Fragments arranged by size: 2683 2658 1522 1396 1183 969 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3682 9229 9416 1700 Size: 110 1872 5547 187 2869 Fragments arranged by size: 5547 2869 1872 187 110 BsrGI T'GTAC_A Cuts at: 96 6944 96 Size: 6848 3737 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10585 BssSI C'ACGA_G Cuts at: 8645 9657 8645 Size: 1012 9573 BstAPI GCAn_nnn'nTGC Cuts at: 794 2195 2198 2989 3692 4270 794 Size: 1401 3 791 703 578 7109 Fragments arranged by size: 7109 1401 791 703 578 3 BstBI TT'CG_AA Cuts at: 6113 9389 6113 Size: 3276 7309 BstEII G'GTnAC_C Cuts at: 1184 2237 2672 4280 7157 1184 Size: 1053 435 1608 2877 4612 Fragments arranged by size: 4612 2877 1608 1053 435 BstXI CCAn_nnnn'nTGG Cuts at: 2126 4375 5483 2126 Size: 2249 1108 7228 Fragments arranged by size: 7228 2249 1108 BstYI r'GATC_y Cuts at: 1972 2810 3352 5572 7915 8282 8667 8684 10136 Size: 838 542 2220 2343 367 385 17 1452 Cuts at: 10136 10222 10234 10580 1972 Size: 86 12 346 1977 Fragments arranged by size: 2343 2220 1977 1452 838 542 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2997 2997 Size: 10585 Bsu36I CC'TnA_GG Cuts at: 3054 6519 3054 Size: 3465 7120 BtgI C'CryG_G Cuts at: 513 1065 2035 4219 4752 4888 5360 6035 513 Size: 552 970 2184 533 136 472 675 5063 Fragments arranged by size: 5063 2184 970 675 552 533 472 136 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2222 487 Size: 683 56 996 8850 Fragments arranged by size: 8850 996 683 56 BtsI GCAGTG_nn' Cuts at: 2161 2167 2173 3214 4852 7459 7669 8101 8895 Size: 6 6 1041 1638 2607 210 432 794 Cuts at: 8895 8915 2161 Size: 20 3831 Fragments arranged by size: 3831 2607 1638 1041 794 432 210 20 6 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10585 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10550 DraI TTT'AAA Cuts at: 7889 7889 Size: 10585 DraIII CAC_nnn'GTG Cuts at: 2833 3692 2833 Size: 859 9726 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9592 809 Size: 507 8276 1802 Fragments arranged by size: 8276 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2227 2599 2698 Size: 54 1425 50 275 18 397 372 99 Cuts at: 2698 3393 3619 4045 4054 4216 7344 8032 8921 Size: 695 226 426 9 162 3128 688 889 Cuts at: 8921 10550 8 Size: 1629 43 Fragments arranged by size: 3128 1629 1425 889 695 688 426 397 372 275 226 162 99 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 8032 1537 Size: 275 6220 4090 Fragments arranged by size: 6220 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 3735 5360 6164 6218 6297 6303 6534 Size: 729 1653 1625 804 54 79 6 231 Cuts at: 6534 7302 8515 1353 Size: 768 1213 3423 Fragments arranged by size: 3423 1653 1625 1213 804 768 729 231 79 54 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5083 9172 9546 9692 387 Size: 1064 138 3494 4089 374 146 1280 Fragments arranged by size: 4089 3494 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2472 3079 3102 3176 3528 3546 Size: 393 651 189 607 23 74 352 18 Cuts at: 3546 3557 3559 3960 4259 4866 4926 5323 5577 Size: 11 2 401 299 607 60 397 254 Cuts at: 5577 5598 5859 6204 6288 6483 6558 7482 7538 Size: 21 261 345 84 195 75 924 56 Cuts at: 7538 8648 9245 10032 10300 1239 Size: 1110 597 787 268 1524 Fragments arranged by size: 1524 1110 924 787 651 607 607 597 401 397 393 352 345 299 268 261 254 195 189 84 75 74 60 56 23 21 18 11 2 EcoNI CCTnn'n_nnAGG Cuts at: 2588 3463 3978 4262 4465 5232 6183 2588 Size: 875 515 284 203 767 951 6990 Fragments arranged by size: 6990 951 875 767 515 284 203 EcoO109I rG'GnC_Cy Cuts at: 2928 3069 3468 4070 4408 4456 4457 4639 4975 Size: 141 399 602 338 48 1 182 336 Cuts at: 4975 5224 5959 6774 7076 7397 2928 Size: 249 735 815 302 321 6116 Fragments arranged by size: 6116 815 735 602 399 338 336 321 302 249 182 141 48 1 EcoRI G'AATT_C Cuts at: 5242 7894 8272 5242 Size: 2652 378 7555 Fragments arranged by size: 7555 2652 378 EcoRV GAT'ATC Cuts at: 5926 5926 Size: 10585 FspI TGC'GCA Cuts at: 9091 9091 Size: 10585 HaeII r_GCGC'y Cuts at: 1678 3762 5491 5880 7049 7372 7640 7689 9732 Size: 2084 1729 389 1169 323 268 49 2043 Cuts at: 9732 1678 Size: 2531 Fragments arranged by size: 2531 2084 2043 1729 1169 389 323 268 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2389 2421 3268 3300 3866 Size: 32 135 32 817 32 847 32 566 Cuts at: 3866 3898 4004 4036 4504 4536 4538 4570 4587 Size: 32 106 32 468 32 2 32 17 Cuts at: 4587 4617 4619 4649 5915 5947 6146 6178 9226 Size: 30 2 30 1266 32 199 32 3048 Cuts at: 9226 9258 9300 9332 1373 Size: 32 42 32 2626 Fragments arranged by size: 3048 2626 1266 847 817 566 468 199 135 106 42 32 32 32 32 32 32 32 32 32 32 32 32 30 30 17 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 7929 8175 669 Size: 988 281 5991 246 3079 Fragments arranged by size: 5991 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 10585 HpaI GTT'AAC Cuts at: 8175 8175 Size: 10585 KasI G'GCGC_C Cuts at: 7368 7685 7368 Size: 317 10268 KpnI G_GTAC'C Cuts at: 3335 7910 3335 Size: 4575 6010 MfeI C'AATT_G Cuts at: 8184 8184 Size: 10585 MluI A'CGCG_T Cuts at: 10461 10461 Size: 10585 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3287 3807 4047 4733 7854 9699 9883 1305 Size: 1982 520 240 686 3121 1845 184 2007 Fragments arranged by size: 3121 2007 1982 1845 686 520 240 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2229 2601 2700 3395 3621 Size: 54 1425 343 397 372 99 695 226 Cuts at: 3621 4047 4056 4218 10 Size: 426 9 162 6377 Fragments arranged by size: 6377 1425 695 426 397 372 343 226 162 99 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 4668 4964 5217 7235 7266 8543 8902 Size: 648 3502 296 253 2018 31 1277 359 Cuts at: 8902 9061 518 Size: 159 2042 Fragments arranged by size: 3502 2042 2018 1277 648 359 296 253 159 31 MspA1I CmG'CkG Cuts at: 1606 2195 2641 3122 3535 4067 4890 5251 5310 Size: 589 446 481 413 532 823 361 59 Cuts at: 5310 5607 8001 8678 9826 10071 1606 Size: 297 2394 677 1148 245 2120 Fragments arranged by size: 2394 2120 1148 823 677 589 532 481 446 413 361 297 245 59 NarI GG'CG_CC Cuts at: 7369 7686 7369 Size: 317 10268 NcoI C'CATG_G Cuts at: 513 1065 4219 4752 513 Size: 552 3154 533 6346 Fragments arranged by size: 6346 3154 552 533 NdeI CA'TA_TG Cuts at: 387 387 Size: 10585 NheI G'CTAG_C Cuts at: 1052 2213 4400 1052 Size: 1161 2187 7237 Fragments arranged by size: 7237 2187 1161 NotI GC'GGCC_GC Cuts at: 8032 8032 Size: 10585 NsiI A_TGCA'T Cuts at: 3955 3955 Size: 10585 NspI r_CATG'y Cuts at: 1132 1229 2987 3424 3772 6356 7943 9488 1132 Size: 97 1758 437 348 2584 1587 1545 2229 Fragments arranged by size: 2584 2229 1758 1587 1545 437 348 97 PasI CC'CwG_GG Cuts at: 1348 2368 4461 4643 5081 6451 1348 Size: 1020 2093 182 438 1370 5482 Fragments arranged by size: 5482 2093 1370 1020 438 182 PciI A'CATG_T Cuts at: 3420 9484 3420 Size: 6064 4521 PflMI CCAn_nnn'nTGG Cuts at: 1108 6865 1108 Size: 5757 4828 PfoI T'CCnGG_A Cuts at: 3976 4108 5614 3976 Size: 132 1506 8947 Fragments arranged by size: 8947 1506 132 PmeI GTTT'AAAC Cuts at: 7889 7889 Size: 10585 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5296 5328 8654 8686 10167 10199 5296 Size: 32 3326 32 1481 32 5682 Fragments arranged by size: 5682 3326 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2928 3069 4639 4975 5224 5959 7076 2928 Size: 141 1570 336 249 735 1117 6437 Fragments arranged by size: 6437 1570 1117 735 336 249 141 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10585 PsiI TTA'TAA Cuts at: 5259 8155 5259 Size: 2896 7689 PspOMI G'GGCC_C Cuts at: 4069 4456 6774 4069 Size: 387 2318 7880 Fragments arranged by size: 7880 2318 387 PstI C_TGCA'G Cuts at: 830 3304 3904 4066 4266 5338 6718 7074 7632 Size: 2474 600 162 200 1072 1380 356 558 Cuts at: 7632 7937 830 Size: 305 3478 Fragments arranged by size: 3478 2474 1380 1072 600 558 356 305 200 162 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10585 PvuII CAG'CTG Cuts at: 2195 3122 3535 5251 2195 Size: 927 413 1716 7529 Fragments arranged by size: 7529 1716 927 413 SacII CC_GC'GG Cuts at: 4891 4891 Size: 10585 SalI G'TCGA_C Cuts at: 1936 7927 1936 Size: 5991 4594 SanDI GG'GwC_CC Cuts at: 2928 4639 4975 5224 2928 Size: 1711 336 249 8289 Fragments arranged by size: 8289 1711 336 249 SapI GCTCTTCn'nnn_ Cuts at: 7302 7302 Size: 10585 SbfI CC_TGCA'GG Cuts at: 4266 7632 7937 4266 Size: 3366 305 6914 Fragments arranged by size: 6914 3366 305 ScaI AGT'ACT Cuts at: 3385 4318 8833 10545 3385 Size: 933 4515 1712 3425 Fragments arranged by size: 4515 3425 1712 933 SexAI A'CCwGG_T Cuts at: 5698 7110 7602 5698 Size: 1412 492 8681 Fragments arranged by size: 8681 1412 492 SfcI C'TryA_G Cuts at: 826 1045 2304 2627 3148 3300 3900 4062 4123 Size: 219 1259 323 521 152 600 162 61 Cuts at: 4123 4188 4262 5334 6066 6714 7070 7628 7933 Size: 65 74 1072 732 648 356 558 305 Cuts at: 7933 9068 9749 9940 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1259 1135 1072 732 681 648 600 558 521 356 323 305 219 191 162 152 74 65 61 SfiI GGCCn_nnn'nGGCC Cuts at: 2341 4053 2341 Size: 1712 8873 SfoI GGC'GCC Cuts at: 7370 7687 7370 Size: 317 10268 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10585 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10585 SmaI CCC'GGG Cuts at: 1872 3833 7912 1872 Size: 1961 4079 4545 Fragments arranged by size: 4545 4079 1961 SmlI C'TyrA_G Cuts at: 820 1017 1943 3387 4320 4818 8689 9590 9852 Size: 197 926 1444 933 498 3871 901 262 Cuts at: 9852 820 Size: 1553 Fragments arranged by size: 3871 1553 1444 933 926 901 498 262 197 SnaBI TAC'GTA Cuts at: 493 8376 493 Size: 7883 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10585 SphI G_CATG'C Cuts at: 1132 7943 1132 Size: 6811 3774 SspI AAT'ATT Cuts at: 5 52 6094 8509 5 Size: 47 6042 2415 2081 Fragments arranged by size: 6042 2415 2081 47 StuI AGG'CCT Cuts at: 1517 2335 1517 Size: 818 9767 StyI C'CwwG_G Cuts at: 513 1065 2440 4219 4752 5372 5955 6296 6430 Size: 552 1375 1779 533 620 583 341 134 Cuts at: 6430 6531 7692 8024 513 Size: 101 1161 332 3074 Fragments arranged by size: 3074 1779 1375 1161 620 583 552 533 341 332 134 101 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4714 5004 6443 6872 7145 7392 8612 Size: 1255 3134 290 1439 429 273 247 1220 Cuts at: 8612 8629 8782 8967 9412 325 Size: 17 153 185 445 1498 Fragments arranged by size: 3134 1498 1439 1255 1220 445 429 290 273 247 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3042 3383 4316 Size: 274 80 33 51 1443 1065 341 933 Cuts at: 4316 6944 8831 10543 96 Size: 2628 1887 1712 138 Fragments arranged by size: 2628 1887 1712 1443 1065 933 341 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2060 2092 2817 2849 7068 7100 7141 7173 10545 Size: 32 725 32 4219 32 41 32 3372 Cuts at: 10545 10577 2060 Size: 32 2068 Fragments arranged by size: 4219 3372 2068 725 41 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6251 7090 1316 Size: 4935 839 4811 Fragments arranged by size: 4935 4811 839 XbaI T'CTAG_A Cuts at: 7921 7921 Size: 10585 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5657 6862 5657 Size: 1205 9380 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10585 XmaI C'CCGG_G Cuts at: 1870 3831 7910 1870 Size: 1961 4079 4545 Fragments arranged by size: 4545 4079 1961 XmnI GAAnn'nnTTC Cuts at: 2335 4272 8714 2335 Size: 1937 4442 4206 Fragments arranged by size: 4442 4206 1937 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8393 276 Size: 53 83 186 936 6859 2468 Fragments arranged by size: 6859 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BsiWI EcoICRI FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SrfI SwaI