(Circular) (Six-base) MAPSORT of: pFN21AA0129.seq Check: 7145 from: 1 to: 6031 pFN21AA0129 6031 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3252 3252 Size: 6031 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3841 278 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3374 1937 Size: 1437 4594 Acc65I G'GTAC_C Cuts at: 2747 3352 2747 Size: 605 5426 AclI AA'CG_TT Cuts at: 1563 4158 4531 1563 Size: 2595 373 3063 Fragments arranged by size: 3063 2595 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 2522 4094 5478 5746 1632 Size: 725 165 1572 1384 268 1917 Fragments arranged by size: 1917 1572 1384 725 268 165 AfeI AGC'GCT Cuts at: 2385 3101 2385 Size: 716 5315 AflII C'TTAA_G Cuts at: 820 1017 2696 820 Size: 197 1679 4155 Fragments arranged by size: 4155 1679 197 AflIII A'CryG_T Cuts at: 1203 4930 5907 1203 Size: 3727 977 1327 Fragments arranged by size: 3727 1327 977 AgeI A'CCGG_T Cuts at: 4862 4862 Size: 6031 AhdI GACnn_n'nnGTC Cuts at: 4760 4760 Size: 6031 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6031 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3164 1184 Size: 1980 4051 AlwNI CAG_nnn'CTG Cuts at: 2499 3774 5346 2499 Size: 1275 1572 3184 Fragments arranged by size: 3184 1572 1275 ApaI G_GGCC'C Cuts at: 2901 2901 Size: 6031 ApaLI G'TGCA_C Cuts at: 2201 4088 5244 2201 Size: 1887 1156 2988 Fragments arranged by size: 2988 1887 1156 ApoI r'AATT_y Cuts at: 1493 2345 2396 3340 3566 3718 5827 1493 Size: 852 51 944 226 152 2109 1697 Fragments arranged by size: 2109 1697 944 852 226 152 51 AseI AT'TA_AT Cuts at: 160 1032 4585 160 Size: 872 3553 1606 Fragments arranged by size: 3553 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 AvaI C'yCGr_G Cuts at: 1870 1943 2585 3356 1870 Size: 73 642 771 4545 Fragments arranged by size: 4545 771 642 73 BamHI G'GATC_C Cuts at: 2818 3361 3728 2818 Size: 543 367 5121 Fragments arranged by size: 5121 543 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2747 2999 3039 3090 3352 Size: 325 217 627 960 252 40 51 262 Cuts at: 3352 4807 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 960 627 325 262 252 217 51 40 BanII G_rGCy'C Cuts at: 1391 2106 2520 2625 2901 3350 1391 Size: 715 414 105 276 449 4072 Fragments arranged by size: 4072 715 449 414 276 105 BbeI G_GCGC'C Cuts at: 3003 3043 3003 Size: 40 5991 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3175 928 Size: 971 1276 3784 Fragments arranged by size: 3784 1276 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4222 4256 1903 Size: 34 2285 34 3678 Fragments arranged by size: 3678 2285 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3923 5133 3923 Size: 1210 4821 BclI T'GATC_A Cuts at: 1548 1548 Size: 6031 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2886 4642 136 Size: 107 122 71 631 605 1214 1756 1525 Fragments arranged by size: 1756 1525 1214 631 605 122 107 71 BglII A'GATC_T Cuts at: 6026 6026 Size: 6031 BlpI GC'TnA_GC Cuts at: 2100 3448 2100 Size: 1348 4683 Bme1580I G_kGCm'C Cuts at: 1790 2205 2610 2901 2907 4092 5248 1790 Size: 415 405 291 6 1185 1156 2573 Fragments arranged by size: 2573 1185 1156 415 405 291 6 BmgBI CAC'GTC Cuts at: 1318 2986 3223 1318 Size: 1668 237 4126 Fragments arranged by size: 4126 1668 237 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2983 3127 3762 Size: 416 53 83 399 24 1566 144 635 Cuts at: 3762 4720 442 Size: 958 1753 Fragments arranged by size: 1753 1566 958 635 416 399 144 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2411 2521 4691 1239 Size: 971 201 110 2170 2579 Fragments arranged by size: 2579 2170 971 201 110 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2288 3080 3131 4156 5021 5319 2288 Size: 792 51 1025 865 298 3000 Fragments arranged by size: 3000 1025 865 792 298 51 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4694 882 Size: 617 3195 2219 Fragments arranged by size: 3195 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2206 3822 493 Size: 711 1002 1616 2702 Fragments arranged by size: 2702 1616 1002 711 BsaBI GATnn'nnATC Cuts at: 6025 6025 Size: 6031 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2678 3000 3040 3180 Size: 53 83 186 936 1145 322 40 140 Cuts at: 3180 3204 3838 4220 4855 275 Size: 24 634 382 635 1451 Fragments arranged by size: 1451 1145 936 635 634 382 322 186 140 83 53 40 24 BsaWI w'CCGG_w Cuts at: 4464 4862 5136 5283 5694 4464 Size: 398 274 147 411 4801 Fragments arranged by size: 4801 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5806 Fragments arranged by size: 5806 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6031 BseYI C'CCAG_C Cuts at: 2152 2884 5234 5730 2152 Size: 732 2350 496 2453 Fragments arranged by size: 2453 2350 732 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2228 3008 1163 Size: 573 492 780 4186 Fragments arranged by size: 4186 780 573 492 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 2092 2860 2965 3057 3172 Size: 275 186 21 70 768 105 92 115 Cuts at: 3172 3481 4242 5270 1540 Size: 309 761 1028 2301 Fragments arranged by size: 2301 1028 768 761 309 275 186 115 105 92 70 21 BsiHKAI G_wGCw'C Cuts at: 2106 2205 4092 4177 5248 2106 Size: 99 1887 85 1071 2889 Fragments arranged by size: 2889 1887 1071 99 85 BsmI GAATG_Cn' Cuts at: 3542 3635 3542 Size: 93 5938 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2205 2520 2610 2625 2901 2907 Size: 399 316 99 315 90 15 276 6 Cuts at: 2907 3350 4092 4177 5248 1391 Size: 443 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 443 399 316 315 276 99 90 85 15 6 BspEI T'CCGG_A Cuts at: 5694 5694 Size: 6031 BspHI T'CATG_A Cuts at: 2475 3920 5650 2475 Size: 1445 1730 2856 Fragments arranged by size: 2856 1730 1445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3252 3386 844 Size: 2408 134 3489 Fragments arranged by size: 3489 2408 134 BsrBI CCG'CTC Cuts at: 1394 1823 3277 3918 1394 Size: 429 1454 641 3507 Fragments arranged by size: 3507 1454 641 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4526 4700 65 Size: 1183 3278 174 1396 Fragments arranged by size: 3278 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2960 3002 3171 3200 4675 4862 1700 Size: 110 1150 42 169 29 1475 187 2869 Fragments arranged by size: 2869 1475 1150 187 169 110 42 29 BsrGI T'GTAC_A Cuts at: 96 2433 96 Size: 2337 3694 BssHII G'CGCG_C Cuts at: 1925 2136 2157 2801 2837 1925 Size: 211 21 644 36 5119 Fragments arranged by size: 5119 644 211 36 21 BssSI C'ACGA_G Cuts at: 2648 2943 4091 5103 2648 Size: 295 1148 1012 3576 Fragments arranged by size: 3576 1148 1012 295 BstAPI GCAn_nnn'nTGC Cuts at: 794 2566 2712 794 Size: 1772 146 4113 Fragments arranged by size: 4113 1772 146 BstBI TT'CG_AA Cuts at: 4835 4835 Size: 6031 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6031 BstYI r'GATC_y Cuts at: 1972 2818 3361 3728 4113 4130 5582 5668 5680 Size: 846 543 367 385 17 1452 86 12 Cuts at: 5680 6026 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 846 543 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2164 2658 3043 513 Size: 552 939 160 494 385 3501 Fragments arranged by size: 3501 939 552 494 385 160 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5292 Fragments arranged by size: 5292 683 56 BtsI GCAGTG_nn' Cuts at: 3547 4341 4361 3547 Size: 794 20 5217 Fragments arranged by size: 5217 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5996 DraI TTT'AAA Cuts at: 2232 3335 2232 Size: 1103 4928 DraIII CAC_nnn'GTG Cuts at: 2176 2176 Size: 6031 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5038 809 Size: 507 3722 1802 Fragments arranged by size: 3722 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2167 2388 2550 Size: 54 1425 50 275 18 337 221 162 Cuts at: 2550 2962 3054 3133 3478 4367 5996 8 Size: 412 92 79 345 889 1629 43 Fragments arranged by size: 1629 1425 889 412 345 337 275 221 162 92 79 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2962 3054 3478 1537 Size: 275 1150 92 424 4090 Fragments arranged by size: 4090 1150 424 275 92 EarI CTCTTCn'nnn_ Cuts at: 1353 2111 2370 3961 1353 Size: 758 259 1591 3423 Fragments arranged by size: 3423 1591 758 259 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2842 4618 4992 5138 387 Size: 1064 138 1253 1776 374 146 1280 Fragments arranged by size: 1776 1280 1253 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2104 2104 Size: 6031 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2357 2411 2521 2522 4094 4691 Size: 393 578 147 54 110 1 1572 597 Cuts at: 4691 5478 5746 1239 Size: 787 268 1524 Fragments arranged by size: 1572 1524 787 597 578 393 268 147 110 54 1 EcoO109I rG'GnC_Cy Cuts at: 2644 2897 3048 2644 Size: 253 151 5627 Fragments arranged by size: 5627 253 151 EcoRI G'AATT_C Cuts at: 3340 3718 3340 Size: 378 5653 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2653 2653 Size: 6031 FspI TGC'GCA Cuts at: 4537 4537 Size: 6031 HaeII r_GCGC'y Cuts at: 1678 2074 2189 2387 3003 3043 3103 5178 1678 Size: 396 115 198 616 40 60 2075 2531 Fragments arranged by size: 2531 2075 616 396 198 115 60 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4672 4704 4746 4778 1373 Size: 32 135 32 3100 32 42 32 2626 Fragments arranged by size: 3100 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2983 3375 3621 669 Size: 988 281 1045 392 246 3079 Fragments arranged by size: 3079 1045 988 392 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6031 HpaI GTT'AAC Cuts at: 3621 3621 Size: 6031 KasI G'GCGC_C Cuts at: 2999 3039 2999 Size: 40 5991 KpnI G_GTAC'C Cuts at: 2751 3356 2751 Size: 605 5426 MfeI C'AATT_G Cuts at: 3630 3630 Size: 6031 MluI A'CGCG_T Cuts at: 5907 5907 Size: 6031 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2025 2514 5145 5329 1305 Size: 720 489 2631 184 2007 Fragments arranged by size: 2631 2007 720 489 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2169 2390 10 Size: 54 1425 343 337 221 3651 Fragments arranged by size: 3651 1425 343 337 221 54 MslI CAynn'nnrTG Cuts at: 518 1166 3989 4348 4507 518 Size: 648 2823 359 159 2042 Fragments arranged by size: 2823 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2118 2127 2198 2239 3045 3447 4124 5272 Size: 512 9 71 41 806 402 677 1148 Cuts at: 5272 5517 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 806 677 512 402 245 71 41 9 NaeI GCC'GGC Cuts at: 3004 3202 3004 Size: 198 5833 NarI GG'CG_CC Cuts at: 3000 3040 3000 Size: 40 5991 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4540 Fragments arranged by size: 4540 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6031 NgoMIV G'CCGG_C Cuts at: 3002 3200 3002 Size: 198 5833 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6031 NotI GC'GGCC_GC Cuts at: 3478 3478 Size: 6031 NspI r_CATG'y Cuts at: 1132 1229 3389 4934 1132 Size: 97 2160 1545 2229 Fragments arranged by size: 2229 2160 1545 97 PasI CC'CwG_GG Cuts at: 1348 2036 1348 Size: 688 5343 PciI A'CATG_T Cuts at: 4930 4930 Size: 6031 PflMI CCAn_nnn'nTGG Cuts at: 1108 2176 1108 Size: 1068 4963 PfoI T'CCnGG_A Cuts at: 3186 3267 3186 Size: 81 5950 PmeI GTTT'AAAC Cuts at: 3335 3335 Size: 6031 PmlI CAC'GTG Cuts at: 2206 2206 Size: 6031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4100 4132 5613 5645 4100 Size: 32 1481 32 4486 Fragments arranged by size: 4486 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6031 PsiI TTA'TAA Cuts at: 3601 3601 Size: 6031 PspOMI G'GGCC_C Cuts at: 2897 2897 Size: 6031 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2346 2378 2346 Size: 32 5999 PstI C_TGCA'G Cuts at: 830 3383 830 Size: 2553 3478 PvuI CG_AT'CG Cuts at: 2001 2860 2001 Size: 859 5172 PvuII CAG'CTG Cuts at: 2239 2239 Size: 6031 SacI G_AGCT'C Cuts at: 2106 2106 Size: 6031 SacII CC_GC'GG Cuts at: 3046 3046 Size: 6031 SalI G'TCGA_C Cuts at: 1936 3373 1936 Size: 1437 4594 SapI GCTCTTCn'nnn_ Cuts at: 2111 2111 Size: 6031 SbfI CC_TGCA'GG Cuts at: 3383 3383 Size: 6031 ScaI AGT'ACT Cuts at: 3116 4279 5991 3116 Size: 1163 1712 3156 Fragments arranged by size: 3156 1712 1163 SfcI C'TryA_G Cuts at: 826 1045 3379 4514 5195 5386 826 Size: 219 2334 1135 681 191 1471 Fragments arranged by size: 2334 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3001 3041 3001 Size: 40 5991 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6031 SmaI CCC'GGG Cuts at: 1872 3358 1872 Size: 1486 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2303 2696 3095 3110 4135 5036 Size: 197 926 360 393 399 15 1025 901 Cuts at: 5036 5298 820 Size: 262 1553 Fragments arranged by size: 1553 1025 926 901 399 393 360 262 197 15 SnaBI TAC'GTA Cuts at: 493 3822 493 Size: 3329 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6031 SphI G_CATG'C Cuts at: 1132 3389 1132 Size: 2257 3774 SspI AAT'ATT Cuts at: 5 52 3955 5 Size: 47 3903 2081 Fragments arranged by size: 3903 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6031 StyI C'CwwG_G Cuts at: 513 1065 2004 3470 513 Size: 552 939 1466 3074 Fragments arranged by size: 3074 1466 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3001 4058 4075 4228 4413 4858 325 Size: 1255 1421 1057 17 153 185 445 1498 Fragments arranged by size: 1498 1421 1255 1057 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2433 2666 3114 Size: 274 80 33 51 1443 456 233 448 Cuts at: 3114 4277 5989 96 Size: 1163 1712 138 Fragments arranged by size: 1712 1443 1163 456 448 274 233 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2803 2835 5991 6023 2803 Size: 32 3156 32 2811 Fragments arranged by size: 3156 2811 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6031 XbaI T'CTAG_A Cuts at: 3367 3367 Size: 6031 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6031 XmaI C'CCGG_G Cuts at: 1870 3356 1870 Size: 1486 4545 XmnI GAAnn'nnTTC Cuts at: 4160 4160 Size: 6031 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3839 276 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI PpuMI PspXI RsrII SanDI SexAI SfiI SrfI SwaI XcmI