(Circular) (Six-base) MAPSORT of: pFN21AB5271.seq Check: 6068 from: 1 to: 5956 pFN21AB5271 5956 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3052 3052 Size: 5956 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3154 3299 1937 Size: 1217 145 4594 Fragments arranged by size: 4594 1217 145 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2290 2684 4019 5403 5671 1632 Size: 658 394 1335 1384 268 1917 Fragments arranged by size: 1917 1384 1335 658 394 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2859 1184 Size: 1675 4281 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2457 2489 2457 Size: 32 5924 AlwNI CAG_nnn'CTG Cuts at: 3699 5271 3699 Size: 1572 4384 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 2213 2693 2808 2908 3030 3265 3491 3643 Size: 720 480 115 100 122 235 226 152 Cuts at: 3643 5752 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 720 480 235 226 152 122 115 100 AseI AT'TA_AT Cuts at: 160 1032 2403 4510 160 Size: 872 1371 2107 1606 Fragments arranged by size: 2107 1606 1371 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 3281 1870 Size: 73 1338 4545 Fragments arranged by size: 4545 1338 73 AvrII C'CTAG_G Cuts at: 2500 2500 Size: 5956 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3277 4732 618 Size: 325 217 627 1490 1455 1842 Fragments arranged by size: 1842 1490 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3275 1391 Size: 1884 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2048 928 Size: 971 149 4836 Fragments arranged by size: 4836 971 149 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4147 4181 1903 Size: 34 50 34 2126 34 3678 Fragments arranged by size: 3678 2126 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4567 136 Size: 107 122 71 631 605 2895 1525 Fragments arranged by size: 2895 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 4017 5173 1790 Size: 2227 1156 2573 Fragments arranged by size: 2573 2227 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2465 3687 4645 Size: 416 53 83 399 24 1048 1222 958 Cuts at: 4645 442 Size: 1753 Fragments arranged by size: 1753 1222 1048 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2333 2740 2333 Size: 407 5549 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2115 4616 1239 Size: 876 2501 2579 Fragments arranged by size: 2579 2501 876 Bpu10I CC'TnA_GC Cuts at: 2151 2151 Size: 5956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 4946 5244 4081 Size: 865 298 4793 Fragments arranged by size: 4793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2253 4619 882 Size: 617 754 2366 2219 Fragments arranged by size: 2366 2219 754 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3763 4145 4780 275 Size: 53 83 186 936 2230 382 635 1451 Fragments arranged by size: 2230 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2611 4389 4787 5061 5208 5619 2611 Size: 1778 398 274 147 411 2948 Fragments arranged by size: 2948 1778 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5731 Fragments arranged by size: 5731 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5956 BseYI C'CCAG_C Cuts at: 2141 5159 5655 2141 Size: 3018 496 2442 Fragments arranged by size: 3018 2442 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5383 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2052 3406 4167 5195 1540 Size: 275 186 51 1354 761 1028 2301 Fragments arranged by size: 2301 1354 1028 761 275 186 51 BsiHKAI G_wGCw'C Cuts at: 2494 4017 4102 5173 2494 Size: 1523 85 1071 3277 Fragments arranged by size: 3277 1523 1071 85 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2494 3275 4017 4102 5173 1391 Size: 399 704 781 742 85 1071 2174 Fragments arranged by size: 2174 1071 781 742 704 399 85 BspEI T'CCGG_A Cuts at: 2611 5619 2611 Size: 3008 2948 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3052 3311 844 Size: 2208 259 3489 Fragments arranged by size: 3489 2208 259 BsrBI CCG'CTC Cuts at: 1394 1823 2011 2031 3843 1394 Size: 429 188 20 1812 3507 Fragments arranged by size: 3507 1812 429 188 20 BsrDI GCAATG_nn' Cuts at: 65 1248 4451 4625 65 Size: 1183 3203 174 1396 Fragments arranged by size: 3203 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4600 4787 1700 Size: 110 2790 187 2869 Fragments arranged by size: 2869 2790 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 2322 4016 5028 2322 Size: 1694 1012 3250 Fragments arranged by size: 3250 1694 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5956 BstBI TT'CG_AA Cuts at: 3028 4760 3028 Size: 1732 4224 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstYI r'GATC_y Cuts at: 1972 3286 3653 4038 4055 5507 5593 5605 5951 Size: 1314 367 385 17 1452 86 12 346 Cuts at: 5951 1972 Size: 1977 Fragments arranged by size: 1977 1452 1314 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3155 3155 Size: 5956 BtgI C'CryG_G Cuts at: 513 1065 2412 513 Size: 552 1347 4057 Fragments arranged by size: 4057 1347 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5217 Fragments arranged by size: 5217 683 56 BtsI GCAGTG_nn' Cuts at: 3472 4266 4286 3472 Size: 794 20 5142 Fragments arranged by size: 5142 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3403 4292 5921 Size: 54 1425 50 275 18 1573 889 1629 Cuts at: 5921 8 Size: 43 Fragments arranged by size: 1629 1573 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2391 2520 2826 3227 3886 1353 Size: 1038 129 306 401 659 3423 Fragments arranged by size: 3423 1038 659 401 306 129 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4543 4917 5063 387 Size: 1064 138 2954 374 146 1280 Fragments arranged by size: 2954 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2290 2684 4019 4616 5403 5671 Size: 393 483 175 394 1335 597 787 268 Cuts at: 5671 1239 Size: 1524 Fragments arranged by size: 1524 1335 787 597 483 394 393 268 175 EcoNI CCTnn'n_nnAGG Cuts at: 2066 2066 Size: 5956 EcoO109I rG'GnC_Cy Cuts at: 2020 2020 Size: 5956 EcoRI G'AATT_C Cuts at: 2213 3030 3265 3643 2213 Size: 817 235 378 4526 Fragments arranged by size: 4526 817 378 235 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3122 3122 Size: 5956 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 5103 1678 Size: 3425 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2305 2336 2337 2368 2664 Size: 32 135 32 733 31 1 31 296 Cuts at: 2664 2696 4597 4629 4671 4703 1373 Size: 32 1901 32 42 32 2626 Fragments arranged by size: 2626 1901 733 296 135 42 32 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 2560 2743 3300 3546 669 Size: 988 281 622 183 557 246 3079 Fragments arranged by size: 3079 988 622 557 281 246 183 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2508 5070 5254 1305 Size: 1203 2562 184 2007 Fragments arranged by size: 2562 2007 1203 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4134 Fragments arranged by size: 4134 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2933 3914 4273 4432 518 Size: 648 1767 981 359 159 2042 Fragments arranged by size: 2042 1767 981 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3372 4049 5197 5442 1606 Size: 1766 677 1148 245 2120 Fragments arranged by size: 2120 1766 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2412 513 Size: 552 1347 4057 Fragments arranged by size: 4057 1347 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 3314 4859 1132 Size: 97 2085 1545 2229 Fragments arranged by size: 2229 2085 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2756 1108 Size: 1648 4308 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2048 1613 Size: 435 5521 PsiI TTA'TAA Cuts at: 3138 3526 3138 Size: 388 5568 PstI C_TGCA'G Cuts at: 830 3308 830 Size: 2478 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SapI GCTCTTCn'nnn_ Cuts at: 2391 2520 2826 2391 Size: 129 306 5521 Fragments arranged by size: 5521 306 129 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SfcI C'TryA_G Cuts at: 826 1045 3304 4439 5120 5311 826 Size: 219 2259 1135 681 191 1471 Fragments arranged by size: 2259 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4060 4961 5223 820 Size: 197 926 2117 901 262 1553 Fragments arranged by size: 2117 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 2339 2701 2856 3134 3880 5 Size: 47 2287 362 155 278 746 2081 Fragments arranged by size: 2287 2081 746 362 278 155 47 StuI AGG'CCT Cuts at: 1517 2072 1517 Size: 555 5401 StyI C'CwwG_G Cuts at: 513 1065 2412 2500 2779 3395 513 Size: 552 1347 88 279 616 3074 Fragments arranged by size: 3074 1347 616 552 279 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3983 4000 4153 4338 4783 325 Size: 1255 2403 17 153 185 445 1498 Fragments arranged by size: 2403 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2552 4202 5914 Size: 274 80 33 51 1443 575 1650 1712 Cuts at: 5914 96 Size: 138 Fragments arranged by size: 1712 1650 1443 575 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5916 5948 5916 Size: 32 5924 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2854 2854 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 2404 4085 2404 Size: 1681 4275 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI