Restriction map for
pFN21AB5241
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I AlwNI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco52I
EcoRV FseI FspI HapII HgaI
HincII HindII HpaI HpaII KasI
KpnI KspI MluI MluNI MroI
MscI MslI MspA1I MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 1183 GACGTC GACGT^C
AccI 1336 GTCTAC GT^[AC][GT]AC
AcyI 1183 GACGTC G[GA]^CG[CT]C
AflIII 737 ACATGT A^C[GA][CT]GT
AocI 615 CCTAAGG CC^T[ACGT]AGG
Aor51HI 532 AGCGCT AGC^GCT
ApaI 1385 GGGCCC GGGCC^C
AspI 1424 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1385 GGGCCC G[GA]GC[CT]^C
BclI 18 TGATCA T^GATCA
BglII 1305 AGATCT A^GATCT
BmyI 1385 GGGCCC G[AGT]GC[ACT]^C
BsaHI 1183 GACGTC G[GA]^CG[CT]C
BseRI 276 GAGGAG GAGGAG(10/8)
Bsp1286I 1385 GGGCCC G[AGT]GC[ACT]^C
BspLU11I 737 ACATGT A^CATGT
BsrBI 1381 GAGCGG CCGCTC(-3/-3)
BstEII 285 GGTGACC G^GT[ACGT]ACC
BstPI 285 GGTGACC G^GT[ACGT]ACC
BstYI 1305 AGATCT [GA]^GATC[CT]
Bsu36I 615 CCTAAGG CC^T[ACGT]AGG
CfoI 533 GCGC GCG^C
Eco47III 532 AGCGCT AGC^GCT
Eco81I 615 CCTAAGG CC^T[ACGT]AGG
EcoO65I 285 GGTGACC G^GT[ACGT]ACC
EcoRI 130 GAATTC G^AATTC
EcoT22I 1101 ATGCAT ATGCA^T
FauI 1383 GCGGG CCCGC(4/6)
FbaI 18 TGATCA T^GATCA
HaeII 532 AGCGCT [GA]GCGC^[CT]
HhaI 533 GCGC GCG^C
Hin1I 1183 GACGTC G[GA]^CG[CT]C
HinP1I 533 GCGC G^CGC
MaeII 1184 ACGT A^CGT
MfeI 46 CAATTG C^AATTG
MflI 1305 AGATCT [GA]^GATC[CT]
MunI 46 CAATTG C^AATTG
NsiI 1101 ATGCAT ATGCA^T
NspI 737 ACATGT [GA]CATG^[CT]
PflFI 1424 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 640 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1428 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 750 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 992 CTGCAG CTGCA^G
SapI 216 GAAGAGC GCTCTTC(1/4)
ScaI 1373 AGTACT AGT^ACT
TaqI 762 TCGA T^CGA
Tth111I 1424 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 762 TCGA T^CGA
Van91I 640 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1029 TCTAGA T^CTAGA
XhoII 1305 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 897 GATCC GGATC(4/5)
120 GGATC
AvaII 510 GGACC G^G[AT]CC
1429 GGTCC
BglI 560 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
927 GCCTAGAGGGC
BpmI 40 CTGGAG CTGGAG(16/14)
1369 CTGGAG
BsaBI 16 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
897 GATCCAAATC
BsmAI 1392 GAGAC GTCTC(1/5)
1422 GAGAC
DdeI 616 CTAAG C^T[ACGT]AG
1390 CTGAG
DraI 571 TTTAAA TTT^AAA
1206 TTTAAA
DraII 1385 GGGCCCT [GA]G^G[ACGT]CC[CT]
1428 AGGTCCT
EarI 216 GAAGAG CTCTTC(1/4)
1078 GAAGAG
EcoNI 351 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
615 CCTAAGGCAGG
EcoO109I 1385 GGGCCCT [GA]G^G[ACGT]CC[CT]
1428 AGGTCCT
EcoT14I 479 CCAAGG C^C[AT][AT]GG
1326 CCTTGG
Ksp632I 216 GAAGAG CTCTTC(1/4)
1078 GAAGAG
MaeIII 56 GTCAC ^GT[ACGT]AC
286 GTGAC
MamI 16 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
897 GATCCAAATC
NlaIV 510 GGACCC GG[ACGT]^[ACGT]CC
1385 GGGCCC
PleI 113 GAGTC GAGTC(4/5)
279 GAGTC
SfaNI 861 GCATC GCATC(5/9)
1231 GCATC
SfcI 399 CTATAG C^T[GA][CT]AG
992 CTGCAG
SmlI 811 CTTGAG C^T[CT][GA]AG
1317 CTTGAG
StuI 482 AGGCCT AGG^CCT
827 AGGCCT
StyI 479 CCAAGG C^C[AT][AT]GG
1326 CCTTGG
TfiI 526 GAATC G^A[AT]TC
988 GAATC
Tsp45I 56 GTCAC ^GT[GC]AC
286 GTGAC
XcmI 143 CCAAATACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1010 CCACATTTGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BfaI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DpnII EcoRII Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
MaeI MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII RsaI
Sau3AI Sau96I ScrFI Tru9I TseI
Tsp509I TspRI