(Circular) (Six-base) MAPSORT of: pFN21AB5241.seq Check: 9430 from: 1 to: 6139 pFN21AB5241 6139 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3192 3949 278 Size: 53 83 186 936 1656 757 2468 Fragments arranged by size: 2468 1656 936 757 186 83 53 AccI GT'mk_AC Cuts at: 1937 3342 3482 1937 Size: 1405 140 4594 Fragments arranged by size: 4594 1405 140 Acc65I G'GTAC_C Cuts at: 3460 3460 Size: 6139 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2240 2474 2669 2699 2727 3102 4202 5586 Size: 608 234 195 30 28 375 1100 1384 Cuts at: 5586 5854 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1100 608 375 268 234 195 30 28 AfeI AGC'GCT Cuts at: 2539 2539 Size: 6139 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5942 AflIII A'CryG_T Cuts at: 1203 2742 5038 6015 1203 Size: 1539 2296 977 1327 Fragments arranged by size: 2296 1539 1327 977 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 4868 4868 Size: 6139 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6139 AlwNI CAG_nnn'CTG Cuts at: 3882 5454 3882 Size: 1572 4567 ApaI G_GGCC'C Cuts at: 3394 3394 Size: 6139 ApaLI G'TGCA_C Cuts at: 4196 5352 4196 Size: 1156 4983 ApoI r'AATT_y Cuts at: 1493 2135 3088 3170 3448 3674 3826 5935 1493 Size: 642 953 82 278 226 152 2109 1697 Fragments arranged by size: 2109 1697 953 642 278 226 152 82 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 3464 1870 Size: 73 1521 4545 Fragments arranged by size: 4545 1521 73 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3460 4915 618 Size: 325 217 627 1673 1455 1842 Fragments arranged by size: 1842 1673 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3394 3458 1391 Size: 2003 64 4072 Fragments arranged by size: 4072 2003 64 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4330 4364 1903 Size: 34 2393 34 3678 Fragments arranged by size: 3678 2393 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4031 5241 4031 Size: 1210 4929 BclI T'GATC_A Cuts at: 1548 2023 1548 Size: 475 5664 BfrBI ATG'CAT Cuts at: 3108 3108 Size: 6139 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2571 2938 4750 Size: 107 122 71 631 605 899 367 1812 Cuts at: 4750 136 Size: 1525 Fragments arranged by size: 1812 1525 899 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 3310 6134 3310 Size: 2824 3315 BlpI GC'TnA_GC Cuts at: 3556 3556 Size: 6139 Bme1580I G_kGCm'C Cuts at: 1790 3394 4200 5356 1790 Size: 1604 806 1156 2573 Fragments arranged by size: 2573 1604 1156 806 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3870 4828 442 Size: 416 53 83 399 24 2453 958 1753 Fragments arranged by size: 2453 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 3395 4799 1239 Size: 827 1329 1404 2579 Fragments arranged by size: 2579 1404 1329 827 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2837 3343 4264 5129 5427 2837 Size: 506 921 865 298 3549 Fragments arranged by size: 3549 921 865 506 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4802 882 Size: 617 3303 2219 Fragments arranged by size: 3303 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3930 493 Size: 711 2726 2702 Fragments arranged by size: 2726 2702 711 BsaBI GATnn'nnATC Cuts at: 2025 2906 6133 2025 Size: 881 3227 2031 Fragments arranged by size: 3227 2031 881 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3189 3946 4328 4963 Size: 53 83 186 936 1656 757 382 635 Cuts at: 4963 275 Size: 1451 Fragments arranged by size: 1656 1451 936 757 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4572 4970 5244 5391 5802 4572 Size: 398 274 147 411 4909 Fragments arranged by size: 4909 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5914 Fragments arranged by size: 5914 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2296 1186 Size: 1110 5029 BseYI C'CCAG_C Cuts at: 2540 5342 5838 2540 Size: 2802 496 2841 Fragments arranged by size: 2841 2802 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5566 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3589 4350 5378 1540 Size: 275 186 1588 761 1028 2301 Fragments arranged by size: 2301 1588 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4200 4285 5356 4200 Size: 85 1071 4983 Fragments arranged by size: 4983 1071 85 BsmI GAATG_Cn' Cuts at: 3650 3743 3650 Size: 93 6046 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3394 3458 4200 4285 5356 1391 Size: 399 1604 64 742 85 1071 2174 Fragments arranged by size: 2174 1604 1071 742 399 85 64 BspEI T'CCGG_A Cuts at: 5802 5802 Size: 6139 BspHI T'CATG_A Cuts at: 4028 5758 4028 Size: 1730 4409 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3494 844 Size: 2650 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3388 4026 1394 Size: 429 1565 638 3507 Fragments arranged by size: 3507 1565 638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4634 4808 65 Size: 1183 3386 174 1396 Fragments arranged by size: 3386 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4783 4970 1700 Size: 110 2973 187 2869 Fragments arranged by size: 2973 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6139 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6139 BssSI C'ACGA_G Cuts at: 4199 5211 4199 Size: 1012 5127 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6139 BstBI TT'CG_AA Cuts at: 4943 4943 Size: 6139 BstEII G'GTnAC_C Cuts at: 1184 2290 1184 Size: 1106 5033 BstYI r'GATC_y Cuts at: 1972 3310 3469 3836 4221 4238 5690 5776 5788 Size: 1338 159 367 385 17 1452 86 12 Cuts at: 5788 6134 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1338 385 367 346 159 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2621 2621 Size: 6139 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5587 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5400 Fragments arranged by size: 5400 683 56 BtsI GCAGTG_nn' Cuts at: 3006 3655 4449 4469 3006 Size: 649 794 20 4676 Fragments arranged by size: 4676 794 649 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6139 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 2578 3213 3443 2578 Size: 635 230 5274 Fragments arranged by size: 5274 635 230 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5146 809 Size: 507 3830 1802 Fragments arranged by size: 3830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3586 4475 6104 Size: 54 1425 50 275 18 1756 889 1629 Cuts at: 6104 8 Size: 43 Fragments arranged by size: 1756 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3586 1537 Size: 275 1774 4090 Fragments arranged by size: 4090 1774 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2216 3078 4069 1353 Size: 863 862 991 3423 Fragments arranged by size: 3423 991 863 862 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4726 5100 5246 387 Size: 1064 138 3137 374 146 1280 Fragments arranged by size: 3137 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2240 2474 2669 2699 2727 3102 Size: 393 434 174 234 195 30 28 375 Cuts at: 3102 3395 4202 4799 5586 5854 1239 Size: 293 807 597 787 268 1524 Fragments arranged by size: 1524 807 787 597 434 393 375 293 268 234 195 174 30 28 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2624 2360 Size: 264 5875 EcoO109I rG'GnC_Cy Cuts at: 3391 3434 3391 Size: 43 6096 EcoRI G'AATT_C Cuts at: 2135 3448 3826 2135 Size: 1313 378 4448 Fragments arranged by size: 4448 1313 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2509 2509 Size: 6139 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 2541 5286 1678 Size: 863 2745 2531 Fragments arranged by size: 2745 2531 863 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3418 3450 4780 4812 4854 Size: 32 135 32 1846 32 1330 32 42 Cuts at: 4854 4886 1373 Size: 32 2626 Fragments arranged by size: 2626 1846 1330 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3483 3729 669 Size: 988 281 1545 246 3079 Fragments arranged by size: 3079 1545 988 281 246 HindIII A'AGCT_T Cuts at: 748 2384 2681 3319 748 Size: 1636 297 638 3568 Fragments arranged by size: 3568 1636 638 297 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KpnI G_GTAC'C Cuts at: 3464 3464 Size: 6139 MfeI C'AATT_G Cuts at: 2051 3738 2051 Size: 1687 4452 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5253 5437 1305 Size: 3948 184 2007 Fragments arranged by size: 3948 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4317 Fragments arranged by size: 4317 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4097 4456 4615 518 Size: 648 2931 359 159 2042 Fragments arranged by size: 2931 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3555 4232 5380 5625 1606 Size: 1949 677 1148 245 2120 Fragments arranged by size: 2120 1949 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5587 NdeI CA'TA_TG Cuts at: 387 387 Size: 6139 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6139 NotI GC'GGCC_GC Cuts at: 3586 3586 Size: 6139 NsiI A_TGCA'T Cuts at: 3110 3110 Size: 6139 NspI r_CATG'y Cuts at: 1132 1229 2746 3497 5042 1132 Size: 97 1517 751 1545 2229 Fragments arranged by size: 2229 1545 1517 751 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6139 PciI A'CATG_T Cuts at: 2742 5038 2742 Size: 2296 3843 PflMI CCAn_nnn'nTGG Cuts at: 1108 2651 1108 Size: 1543 4596 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4208 4240 5721 5753 4208 Size: 32 1481 32 4594 Fragments arranged by size: 4594 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3434 3434 Size: 6139 PshAI GACnn'nnGTC Cuts at: 1613 2759 1613 Size: 1146 4993 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PspOMI G'GGCC_C Cuts at: 3390 3390 Size: 6139 PstI C_TGCA'G Cuts at: 830 3001 3491 830 Size: 2171 490 3478 Fragments arranged by size: 3478 2171 490 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 SalI G'TCGA_C Cuts at: 1936 3481 1936 Size: 1545 4594 SapI GCTCTTCn'nnn_ Cuts at: 2216 2216 Size: 6139 SbfI CC_TGCA'GG Cuts at: 3491 3491 Size: 6139 ScaI AGT'ACT Cuts at: 3380 4387 6099 3380 Size: 1007 1712 3420 Fragments arranged by size: 3420 1712 1007 SfcI C'TryA_G Cuts at: 826 1045 2404 2997 3487 4622 5303 5494 826 Size: 219 1359 593 490 1135 681 191 1471 Fragments arranged by size: 1471 1359 1135 681 593 490 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6139 SmaI CCC'GGG Cuts at: 1872 3466 1872 Size: 1594 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2816 3322 4243 5144 5406 820 Size: 197 926 873 506 921 901 262 1553 Fragments arranged by size: 1553 926 921 901 873 506 262 197 SnaBI TAC'GTA Cuts at: 493 3930 493 Size: 3437 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 3497 1132 Size: 2365 3774 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 2489 2834 1517 Size: 972 345 4822 Fragments arranged by size: 4822 972 345 StyI C'CwwG_G Cuts at: 513 1065 2484 3331 3578 513 Size: 552 1419 847 247 3074 Fragments arranged by size: 3074 1419 847 552 247 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2863 4166 4183 4336 4521 4966 325 Size: 1255 1283 1303 17 153 185 445 1498 Fragments arranged by size: 1498 1303 1283 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2009 2718 3378 Size: 274 80 33 51 1443 32 709 660 Cuts at: 3378 4385 6097 96 Size: 1007 1712 138 Fragments arranged by size: 1712 1443 1007 709 660 274 138 80 51 33 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2886 2918 6099 6131 2886 Size: 32 3181 32 2894 Fragments arranged by size: 3181 2894 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3432 1316 Size: 2116 4023 XbaI T'CTAG_A Cuts at: 3034 3475 3034 Size: 441 5698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2155 3022 2155 Size: 867 5272 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6139 XmaI C'CCGG_G Cuts at: 1870 3464 1870 Size: 1594 4545 XmnI GAAnn'nnTTC Cuts at: 4268 4268 Size: 6139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3190 3947 276 Size: 53 83 186 936 1656 757 2468 Fragments arranged by size: 2468 1656 936 757 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI