################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AclI AcsI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsiEI BsiWI Bsp1407I BspDI BspHI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoT22I FseI FspI HindIII HpaI MamI MfeI MluI MluNI MscI MunI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 320 GACGTC GACGT^C Acc65I 485 GGTACC G^GTACC AccII 1785 CGCG CG^CG AfaI 486 GTAC GT^AC Asp718I 485 GGTACC G^GTACC AvaI 383 CTCGAG C^[CT]CG[GA]G BbeI 950 GGCGCC GGCGC^C BbrPI 1746 CACGTG CAC^GTG BbsI 1807 GAAGAC GAAGAC(2/6) BglI 1238 GCCAGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1807 GAAGAC GAAGAC(2/6) BsaAI 1746 CACGTG [CT]AC^GT[GA] BsmBI 1260 CGTCTC CGTCTC(1/5) BsmI 151 GAATGC GAATGC(1/-1) BsoBI 383 CTCGAG C^[CT]CG[GA]G BspLU11I 1148 ACATGT A^CATGT BsrBI 568 CCGCTC CCGCTC(-3/-3) BstUI 1785 CGCG CG^CG EarI 1523 CTCTTC CTCTTC(1/4) HincII 1474 GTCAAC GT[CT]^[GA]AC HindII 1474 GTCAAC GT[CT]^[GA]AC KasI 950 GGCGCC G^GCGCC KpnI 485 GGTACC GGTAC^C Ksp632I 1523 CTCTTC CTCTTC(1/4) KspI 1784 CCGCGG CCGC^GG MseI 437 TTAA T^TAA MvnI 1785 CGCG CG^CG NarI 950 GGCGCC GG^CGCC NdeI 955 CATATG CA^TATG NspI 1148 ACATGT [GA]CATG^[CT] PaeR7I 383 CTCGAG C^TCGAG PflMI 1014 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1746 CACGTG CAC^GTG PmlI 1746 CACGTG CAC^GTG PpuMI 1223 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 1514 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 486 GTAC GT^AC SacI 240 GAGCTC GAGCT^C SacII 1784 CCGCGG CCGC^GG SfoI 950 GGCGCC GGC^GCC Tru9I 437 TTAA T^TAA Van91I 1014 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 383 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 107 GTCTAC GT^[AC][GT]AC 846 GTCTAC AflIII 842 ACACGT A^C[GA][CT]GT 1148 ACATGT AocI 429 CCTAAGG CC^T[ACGT]AGG 2156 CCTCAGG AspHI 240 GAGCTC G[AT]GC[AT]^C 979 GTGCTC BbvCI 1642 CCTCAGC CCTCAGC(-5/-2) 1688 CCTCAGC BcgI 278 CGATGACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2074 CGAAGTGGCTGC BclI 665 TGATCA T^GATCA 1007 TGATCA BfaI 346 CTAG C^TAG 1331 CTAG BsgI 798 CTGCAC GTGCAG(16/14) 1162 CTGCAC BsiHKAI 240 GAGCTC G[AT]GC[AT]^C 979 GTGCTC BspMI 197 ACCTGC ACCTGC(4/8) 1199 ACCTGC BsrFI 189 GCCGGC [GA]^CCGG[CT] 1495 GCCGGC BssSI 205 CTCGTG CACGAG(-5/-1) 1597 CTCGTG BstEII 424 GGTGACC G^GT[ACGT]ACC 1269 GGTCACC BstPI 424 GGTGACC G^GT[ACGT]ACC 1269 GGTCACC Bsu36I 429 CCTAAGG CC^T[ACGT]AGG 2156 CCTCAGG Cfr10I 189 GCCGGC [GA]^CCGG[CT] 1495 GCCGGC EaeI 191 CGGCCA [CT]^GGCC[GA] 1787 CGGCCA Eco81I 429 CCTAAGG CC^T[ACGT]AGG 2156 CCTCAGG EcoNI 260 CCTTCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1200 CCTGCAAAAGG EcoO65I 424 GGTGACC G^GT[ACGT]ACC 1269 GGTCACC EcoRV 448 GATATC GAT^ATC 652 GATATC FbaI 665 TGATCA T^GATCA 1007 TGATCA HaeII 183 GGCGCT [GA]GCGC^[CT] 950 GGCGCC MaeI 346 CTAG C^TAG 1331 CTAG NaeI 189 GCCGGC GCC^GGC 1495 GCCGGC NgoMIV 189 GCCGGC G^CCGGC 1495 GCCGGC PleI 1342 GACTC GAGTC(4/5) 1280 GAGTC PvuII 550 CAGCTG CAG^CTG 1044 CAGCTG StuI 268 AGGCCT AGG^CCT 392 AGGCCT TfiI 362 GAATC G^A[AT]TC 417 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccIII AciI AcyI AluI AlwI ApaI AvaII BamHI BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspEI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MroI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII