(Circular) (Six-base) MAPSORT of: pFN21AE4209.seq Check: 7878 from: 1 to: 6892 pFN21AE4209 6892 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2329 4702 278 Size: 53 83 186 936 793 2373 2468 Fragments arranged by size: 2468 2373 936 793 186 83 53 AccI GT'mk_AC Cuts at: 1937 2113 2852 4235 1937 Size: 176 739 1383 4594 Fragments arranged by size: 4594 1383 739 176 Acc65I G'GTAC_C Cuts at: 2490 4213 2490 Size: 1723 5169 AclI AA'CG_TT Cuts at: 1563 5019 5392 1563 Size: 3456 373 3063 Fragments arranged by size: 3456 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2934 4053 4955 6339 6607 1632 Size: 1302 1119 902 1384 268 1917 Fragments arranged by size: 1917 1384 1302 1119 902 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6695 AflIII A'CryG_T Cuts at: 1203 2847 3153 5791 6768 1203 Size: 1644 306 2638 977 1327 Fragments arranged by size: 2638 1644 1327 977 306 AgeI A'CCGG_T Cuts at: 5723 5723 Size: 6892 AhdI GACnn_n'nnGTC Cuts at: 5621 5621 Size: 6892 AleI CACnn'nnGTG Cuts at: 1166 2981 3110 3602 4080 1166 Size: 1815 129 492 478 3978 Fragments arranged by size: 3978 1815 492 478 129 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2999 1184 Size: 1815 5077 AlwNI CAG_nnn'CTG Cuts at: 4635 6207 4635 Size: 1572 5320 ApaI G_GGCC'C Cuts at: 2587 2870 4052 2587 Size: 283 1182 5427 Fragments arranged by size: 5427 1182 283 ApaLI G'TGCA_C Cuts at: 4949 6105 4949 Size: 1156 5736 ApoI r'AATT_y Cuts at: 1493 4201 4427 4579 6688 1493 Size: 2708 226 152 2109 1697 Fragments arranged by size: 2708 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5446 160 Size: 872 4414 1606 Fragments arranged by size: 4414 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6892 AvaI C'yCGr_G Cuts at: 1870 1943 2388 4217 1870 Size: 73 445 1829 4545 Fragments arranged by size: 4545 1829 445 73 BamHI G'GATC_C Cuts at: 3563 3591 3678 3780 4222 4589 3563 Size: 28 87 102 442 367 5866 Fragments arranged by size: 5866 442 367 102 87 28 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2148 2383 2490 2887 2955 Size: 325 217 627 361 235 107 397 68 Cuts at: 2955 4213 5668 618 Size: 1258 1455 1842 Fragments arranged by size: 1842 1455 1258 627 397 361 325 235 217 107 68 BanII G_rGCy'C Cuts at: 1391 2249 2264 2587 2870 3359 3744 4052 4211 Size: 858 15 323 283 489 385 308 159 Cuts at: 4211 1391 Size: 4072 Fragments arranged by size: 4072 858 489 385 323 308 283 159 15 BbeI G_GCGC'C Cuts at: 2959 2959 Size: 6892 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3819 928 Size: 971 1920 4001 Fragments arranged by size: 4001 1920 971 BbvCI CC'TCA_GC Cuts at: 3648 3694 3648 Size: 46 6846 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2272 2306 4068 4102 5083 5117 1903 Size: 34 335 34 1762 34 981 34 3678 Fragments arranged by size: 3678 1762 981 335 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4784 5994 4784 Size: 1210 5682 BclI T'GATC_A Cuts at: 1548 2670 3012 1548 Size: 1122 342 5428 Fragments arranged by size: 5428 1122 342 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3249 5503 136 Size: 107 122 71 631 605 1577 2254 1525 Fragments arranged by size: 2254 1577 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6887 6887 Size: 6892 BlpI GC'TnA_GC Cuts at: 4309 4309 Size: 6892 Bme1580I G_kGCm'C Cuts at: 1790 2042 2153 2587 2870 3468 4052 4953 6109 Size: 252 111 434 283 598 584 901 1156 Cuts at: 6109 1790 Size: 2573 Fragments arranged by size: 2573 1156 901 598 584 434 283 252 111 BmgBI CAC'GTC Cuts at: 1318 2850 1318 Size: 1532 5360 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2701 2975 4623 Size: 416 53 83 399 24 1284 274 1648 Cuts at: 4623 5581 442 Size: 958 1753 Fragments arranged by size: 1753 1648 1284 958 416 399 274 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6892 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3858 3858 Size: 6892 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2821 2980 3078 3566 5552 1239 Size: 815 767 159 98 488 1986 2579 Fragments arranged by size: 2579 1986 815 767 488 159 98 Bpu10I CC'TnA_GC Cuts at: 2236 3257 3648 3694 2236 Size: 1021 391 46 5434 Fragments arranged by size: 5434 1021 391 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2633 3883 4001 5017 5882 6180 2633 Size: 1250 118 1016 865 298 3345 Fragments arranged by size: 3345 1250 1016 865 298 118 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3368 3531 3568 5555 882 Size: 617 1869 163 37 1987 2219 Fragments arranged by size: 2219 1987 1869 617 163 37 BsaAI yAC'GTr Cuts at: 493 1204 3753 4683 493 Size: 711 2549 930 2702 Fragments arranged by size: 2702 2549 930 711 BsaBI GATnn'nnATC Cuts at: 6886 6886 Size: 6892 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2165 2326 2956 3222 Size: 53 83 186 936 632 161 630 266 Cuts at: 3222 3264 4022 4699 5081 5716 275 Size: 42 758 677 382 635 1451 Fragments arranged by size: 1451 936 758 677 635 632 630 382 266 186 161 83 53 42 BsaWI w'CCGG_w Cuts at: 2466 2931 3989 4094 4113 5325 5723 5997 6144 Size: 465 1058 105 19 1212 398 274 147 Cuts at: 6144 6555 2466 Size: 411 2803 Fragments arranged by size: 2803 1212 1058 465 411 398 274 147 105 19 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2703 2733 3207 3237 3346 Size: 30 165 30 1300 30 474 30 109 Cuts at: 3346 3376 3701 3731 1178 Size: 30 325 30 4339 Fragments arranged by size: 4339 1300 474 325 165 109 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3382 3676 3945 1186 Size: 2196 294 269 4133 Fragments arranged by size: 4133 2196 294 269 BseYI C'CCAG_C Cuts at: 2527 2562 3018 3132 4026 4184 6095 6591 2527 Size: 35 456 114 894 158 1911 496 2828 Fragments arranged by size: 2828 1911 894 496 456 158 114 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2788 3152 1163 Size: 573 1052 364 4903 Fragments arranged by size: 4903 1052 573 364 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4342 5103 6131 1540 Size: 275 186 2341 761 1028 2301 Fragments arranged by size: 2341 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2249 2988 4953 5038 6109 2249 Size: 739 1965 85 1071 3032 Fragments arranged by size: 3032 1965 1071 739 85 BsmI GAATG_Cn' Cuts at: 2162 4403 4496 2162 Size: 2241 93 4558 Fragments arranged by size: 4558 2241 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3271 3271 Size: 6892 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2153 2249 2264 2587 2870 2988 Size: 399 252 111 96 15 323 283 118 Cuts at: 2988 3359 3468 3744 4052 4211 4953 5038 6109 Size: 371 109 276 308 159 742 85 1071 Cuts at: 6109 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 371 323 308 283 276 252 159 118 111 109 96 85 15 BspEI T'CCGG_A Cuts at: 2466 3989 4094 4113 6555 2466 Size: 1523 105 19 2442 2803 Fragments arranged by size: 2803 2442 1523 105 19 BspHI T'CATG_A Cuts at: 4781 6511 4781 Size: 1730 5162 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2211 3213 4247 844 Size: 1367 1002 1034 3489 Fragments arranged by size: 3489 1367 1034 1002 BsrBI CCG'CTC Cuts at: 1394 1823 2575 4779 1394 Size: 429 752 2204 3507 Fragments arranged by size: 3507 2204 752 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5387 5561 65 Size: 1183 4139 174 1396 Fragments arranged by size: 4139 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2194 3500 5536 5723 1700 Size: 110 384 1306 2036 187 2869 Fragments arranged by size: 2869 2036 1306 384 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6892 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6892 BssSI C'ACGA_G Cuts at: 2210 3602 4952 5964 2210 Size: 1392 1350 1012 3138 Fragments arranged by size: 3138 1392 1350 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6892 BstBI TT'CG_AA Cuts at: 5696 5696 Size: 6892 BstEII G'GTnAC_C Cuts at: 1184 2429 3274 1184 Size: 1245 845 4802 Fragments arranged by size: 4802 1245 845 BstXI CCAn_nnnn'nTGG Cuts at: 2378 2539 3055 3112 3604 4082 4147 2378 Size: 161 516 57 492 478 65 5123 Fragments arranged by size: 5123 516 492 478 161 65 57 BstYI r'GATC_y Cuts at: 1972 3563 3591 3678 3780 4222 4589 4974 4991 Size: 1591 28 87 102 442 367 385 17 Cuts at: 4991 6443 6529 6541 6887 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1591 1452 442 385 367 346 102 87 86 28 17 12 Bsu36I CC'TnA_GG Cuts at: 2435 4162 2435 Size: 1727 5165 BtgI C'CryG_G Cuts at: 513 1065 2004 2538 3024 3111 3184 3431 3633 Size: 552 939 534 486 87 73 247 202 Cuts at: 3633 3699 3789 513 Size: 66 90 3616 Fragments arranged by size: 3616 939 552 534 486 247 202 90 87 73 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6153 Fragments arranged by size: 6153 683 56 BtsI GCAGTG_nn' Cuts at: 3468 3775 4155 4408 5202 5222 3468 Size: 307 380 253 794 20 5138 Fragments arranged by size: 5138 794 380 307 253 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6892 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6857 DraI TTT'AAA Cuts at: 4196 4196 Size: 6892 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5899 809 Size: 507 4583 1802 Fragments arranged by size: 4583 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 3792 4339 Size: 54 1425 50 275 18 366 1596 547 Cuts at: 4339 5228 6857 8 Size: 889 1629 43 Fragments arranged by size: 1629 1596 1425 889 547 366 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4339 1537 Size: 275 2527 4090 Fragments arranged by size: 4090 2527 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3534 4822 1353 Size: 2181 1288 3423 Fragments arranged by size: 3423 2181 1288 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3556 5479 5853 5999 387 Size: 1064 138 1967 1923 374 146 1280 Fragments arranged by size: 1967 1923 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2247 2247 Size: 6892 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2821 2934 2980 3078 3566 4053 Size: 393 422 767 113 46 98 488 487 Cuts at: 4053 4955 5552 6339 6607 1239 Size: 902 597 787 268 1524 Fragments arranged by size: 1524 902 787 767 597 488 487 422 393 268 113 98 46 EcoNI CCTnn'n_nnAGG Cuts at: 2269 3209 2269 Size: 940 5952 EcoO109I rG'GnC_Cy Cuts at: 2583 2584 2866 3229 2583 Size: 1 282 363 6246 Fragments arranged by size: 6246 363 282 1 EcoRI G'AATT_C Cuts at: 4201 4579 4201 Size: 378 6514 EcoRV GAT'ATC Cuts at: 2455 2659 2455 Size: 204 6688 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3545 3545 Size: 6892 FspI TGC'GCA Cuts at: 5398 5398 Size: 6892 HaeII r_GCGC'y Cuts at: 1678 2192 2959 6039 1678 Size: 514 767 3080 2531 Fragments arranged by size: 3080 2531 767 514 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2442 2474 3207 3239 3276 Size: 32 135 32 870 32 733 32 37 Cuts at: 3276 3308 3346 3378 3583 3615 3701 3733 5533 Size: 32 38 32 205 32 86 32 1800 Cuts at: 5533 5565 5607 5639 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1800 870 733 205 135 86 42 38 37 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3481 4236 4482 669 Size: 988 281 1543 755 246 3079 Fragments arranged by size: 3079 1543 988 755 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6892 HpaI GTT'AAC Cuts at: 4482 4482 Size: 6892 KasI G'GCGC_C Cuts at: 2955 2955 Size: 6892 KpnI G_GTAC'C Cuts at: 2494 4217 2494 Size: 1723 5169 MfeI C'AATT_G Cuts at: 4491 4491 Size: 6892 MluI A'CGCG_T Cuts at: 6768 6768 Size: 6892 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2202 6006 6190 1305 Size: 897 3804 184 2007 Fragments arranged by size: 3804 2007 897 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5070 Fragments arranged by size: 5070 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2376 2666 2981 3110 3602 3881 4080 Size: 648 1210 290 315 129 492 279 199 Cuts at: 4080 4850 5209 5368 518 Size: 770 359 159 2042 Fragments arranged by size: 2042 1210 770 648 492 359 315 290 279 199 159 129 MspA1I CmG'CkG Cuts at: 1606 2517 2557 3051 3791 4308 4985 6133 6378 Size: 911 40 494 740 517 677 1148 245 Cuts at: 6378 1606 Size: 2120 Fragments arranged by size: 2120 1148 911 740 677 517 494 245 40 NaeI GCC'GGC Cuts at: 2196 3502 2196 Size: 1306 5586 NarI GG'CG_CC Cuts at: 2956 2956 Size: 6892 NcoI C'CATG_G Cuts at: 513 1065 2004 3024 3184 3633 513 Size: 552 939 1020 160 449 3772 Fragments arranged by size: 3772 1020 939 552 449 160 NdeI CA'TA_TG Cuts at: 387 2961 387 Size: 2574 4318 NgoMIV G'CCGG_C Cuts at: 2194 3500 2194 Size: 1306 5586 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6892 NotI GC'GGCC_GC Cuts at: 4339 4339 Size: 6892 NspI r_CATG'y Cuts at: 1132 1229 3157 4250 5795 1132 Size: 97 1928 1093 1545 2229 Fragments arranged by size: 2229 1928 1545 1093 97 PasI CC'CwG_GG Cuts at: 1348 2870 3506 1348 Size: 1522 636 4734 Fragments arranged by size: 4734 1522 636 PciI A'CATG_T Cuts at: 3153 5791 3153 Size: 2638 4254 PflMI CCAn_nnn'nTGG Cuts at: 1108 3025 1108 Size: 1917 4975 PfoI T'CCnGG_A Cuts at: 2249 2876 3094 3350 3587 2249 Size: 627 218 256 237 5554 Fragments arranged by size: 5554 627 256 237 218 PmeI GTTT'AAAC Cuts at: 4196 4196 Size: 6892 PmlI CAC'GTG Cuts at: 3753 3753 Size: 6892 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4961 4993 6474 6506 4961 Size: 32 1481 32 5347 Fragments arranged by size: 5347 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3229 3229 Size: 6892 PshAI GACnn'nnGTC Cuts at: 1613 3523 1613 Size: 1910 4982 PsiI TTA'TAA Cuts at: 4462 4462 Size: 6892 PspOMI G'GGCC_C Cuts at: 2583 2866 4048 2583 Size: 283 1182 5427 Fragments arranged by size: 5427 1182 283 PspXI vC'TCGA_Gb Cuts at: 2388 2388 Size: 6892 PstI C_TGCA'G Cuts at: 830 2334 2582 3573 4244 830 Size: 1504 248 991 671 3478 Fragments arranged by size: 3478 1504 991 671 248 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6892 PvuII CAG'CTG Cuts at: 2557 3051 2557 Size: 494 6398 SacI G_AGCT'C Cuts at: 2249 2249 Size: 6892 SacII CC_GC'GG Cuts at: 3792 3792 Size: 6892 SalI G'TCGA_C Cuts at: 1936 4234 1936 Size: 2298 4594 SbfI CC_TGCA'GG Cuts at: 4244 4244 Size: 6892 ScaI AGT'ACT Cuts at: 5140 6852 5140 Size: 1712 5180 SfcI C'TryA_G Cuts at: 826 1045 2114 2330 2410 2578 3569 4240 5375 Size: 219 1069 216 80 168 991 671 1135 Cuts at: 5375 6056 6247 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1069 991 681 671 219 216 191 168 80 SfoI GGC'GCC Cuts at: 2957 2957 Size: 6892 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6892 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6892 SmaI CCC'GGG Cuts at: 1872 4219 1872 Size: 2347 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2388 2648 3898 4016 4996 5897 Size: 197 926 445 260 1250 118 980 901 Cuts at: 5897 6159 820 Size: 262 1553 Fragments arranged by size: 1553 1250 980 926 901 445 262 260 197 118 SnaBI TAC'GTA Cuts at: 493 4683 493 Size: 4190 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6892 SphI G_CATG'C Cuts at: 1132 4250 1132 Size: 3118 3774 SspI AAT'ATT Cuts at: 5 52 4816 5 Size: 47 4764 2081 Fragments arranged by size: 4764 2081 47 StuI AGG'CCT Cuts at: 1517 2275 2399 1517 Size: 758 124 6010 Fragments arranged by size: 6010 758 124 StyI C'CwwG_G Cuts at: 513 1065 2004 3024 3184 3270 3406 3633 4044 Size: 552 939 1020 160 86 136 227 411 Cuts at: 4044 4134 4331 513 Size: 90 197 3074 Fragments arranged by size: 3074 1020 939 552 411 227 197 160 136 90 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3032 4919 4936 5089 5274 5719 325 Size: 1255 1452 1887 17 153 185 445 1498 Fragments arranged by size: 1887 1498 1452 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5138 6850 96 Size: 274 80 33 51 1443 3161 1712 138 Fragments arranged by size: 3161 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3663 3695 3861 3893 3964 3996 6852 6884 3663 Size: 32 166 32 71 32 2856 32 3671 Fragments arranged by size: 3671 2856 166 71 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6892 XbaI T'CTAG_A Cuts at: 4228 4228 Size: 6892 XhoI C'TCGA_G Cuts at: 1943 2388 1943 Size: 445 6447 XmaI C'CCGG_G Cuts at: 1870 4217 1870 Size: 2347 4545 XmnI GAAnn'nnTTC Cuts at: 5021 5021 Size: 6892 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2327 4700 276 Size: 53 83 186 936 793 2373 2468 Fragments arranged by size: 2468 2373 936 793 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII FseI FspAI NruI NsiI PacI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI