(Linear) (Six-base) MAPSORT of: KIEE4209.seq Check: 9156 from: 1 to: 2187 KIEE4209.orf With 182 enzymes: SgfI * July 3, 2012 18:46 .. AatII G_ACGT'C Cuts at: 0 324 2187 Size: 324 1863 AccI GT'mk_AC Cuts at: 0 108 847 2187 Size: 108 739 1340 Fragments arranged by size: 1340 739 108 Acc65I G'GTAC_C Cuts at: 0 485 2187 Size: 485 1702 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 929 2048 2187 Size: 929 1119 139 Fragments arranged by size: 1119 929 139 AflIII A'CryG_T Cuts at: 0 842 1148 2187 Size: 842 306 1039 Fragments arranged by size: 1039 842 306 AleI CACnn'nnGTG Cuts at: 0 976 1105 1597 2075 2187 Size: 976 129 492 478 112 Fragments arranged by size: 976 492 478 129 112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 994 2187 Size: 994 1193 ApaI G_GGCC'C Cuts at: 0 582 865 2047 2187 Size: 582 283 1182 140 Fragments arranged by size: 1182 582 283 140 AvaI C'yCGr_G Cuts at: 0 383 2187 Size: 383 1804 BamHI G'GATC_C Cuts at: 0 1558 1586 1673 1775 2187 Size: 1558 28 87 102 412 Fragments arranged by size: 1558 412 102 87 28 BanI G'GyrC_C Cuts at: 0 143 378 485 882 950 2187 Size: 143 235 107 397 68 1237 Fragments arranged by size: 1237 397 235 143 107 68 BanII G_rGCy'C Cuts at: 0 244 259 582 865 1354 1739 2047 2187 Size: 244 15 323 283 489 385 308 140 Fragments arranged by size: 489 385 323 308 283 244 140 15 BbeI G_GCGC'C Cuts at: 0 954 2187 Size: 954 1233 BbsI GAAGACnn'nnnn_ Cuts at: 0 1814 2187 Size: 1814 373 BbvCI CC'TCA_GC Cuts at: 0 1643 1689 2187 Size: 1643 46 498 Fragments arranged by size: 1643 498 46 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 267 301 2063 2097 2187 Size: 267 34 1762 34 90 Fragments arranged by size: 1762 267 90 34 34 BclI T'GATC_A Cuts at: 0 665 1007 2187 Size: 665 342 1180 Fragments arranged by size: 1180 665 342 BglI GCCn_nnn'nGGC Cuts at: 0 1244 2187 Size: 1244 943 Bme1580I G_kGCm'C Cuts at: 0 37 148 582 865 1463 2047 2187 Size: 37 111 434 283 598 584 140 Fragments arranged by size: 598 584 434 283 140 111 37 BmgBI CAC'GTC Cuts at: 0 845 2187 Size: 845 1342 BmrI ACTGGGnnnn_n' Cuts at: 0 696 970 2187 Size: 696 274 1217 Fragments arranged by size: 1217 696 274 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1853 2187 Size: 1853 334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 816 975 1073 1561 2187 Size: 49 767 159 98 488 626 Fragments arranged by size: 767 626 488 159 98 49 Bpu10I CC'TnA_GC Cuts at: 0 231 1252 1643 1689 2187 Size: 231 1021 391 46 498 Fragments arranged by size: 1021 498 391 231 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 628 1878 1996 2187 Size: 628 1250 118 191 Fragments arranged by size: 1250 628 191 118 BsaI GGTCTCn'nnnn_ Cuts at: 0 1363 1526 1563 2187 Size: 1363 163 37 624 Fragments arranged by size: 1363 624 163 37 BsaAI yAC'GTr Cuts at: 0 1748 2187 Size: 1748 439 BsaHI Gr'CG_yC Cuts at: 0 160 321 951 1217 1259 2017 2187 Size: 160 161 630 266 42 758 170 Fragments arranged by size: 758 630 266 170 161 160 42 BsaWI w'CCGG_w Cuts at: 0 461 926 1984 2089 2108 2187 Size: 461 465 1058 105 19 79 Fragments arranged by size: 1058 465 461 105 79 19 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 698 728 1202 1232 1341 1371 1696 1726 Size: 698 30 474 30 109 30 325 30 Cuts at: 1726 2187 Size: 461 Fragments arranged by size: 698 474 461 325 109 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1377 1671 1940 2187 Size: 1377 294 269 247 Fragments arranged by size: 1377 294 269 247 BseYI C'CCAG_C Cuts at: 0 522 557 1013 1127 2021 2179 2187 Size: 522 35 456 114 894 158 8 Fragments arranged by size: 894 522 456 158 114 35 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 783 1147 2187 Size: 783 364 1040 Fragments arranged by size: 1040 783 364 BsiHKAI G_wGCw'C Cuts at: 0 244 983 2187 Size: 244 739 1204 Fragments arranged by size: 1204 739 244 BsmI GAATG_Cn' Cuts at: 0 157 2187 Size: 157 2030 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1266 2187 Size: 1266 921 Bsp1286I G_dGCh'C Cuts at: 0 37 148 244 259 582 865 983 1354 Size: 37 111 96 15 323 283 118 371 Cuts at: 1354 1463 1739 2047 2187 Size: 109 276 308 140 Fragments arranged by size: 371 323 308 283 276 140 118 111 109 96 37 15 BspEI T'CCGG_A Cuts at: 0 461 1984 2089 2108 2187 Size: 461 1523 105 19 79 Fragments arranged by size: 1523 461 105 79 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 206 1208 2187 Size: 206 1002 979 Fragments arranged by size: 1002 979 206 BsrBI CCG'CTC Cuts at: 0 570 2187 Size: 570 1617 BsrFI r'CCGG_y Cuts at: 0 189 1495 2187 Size: 189 1306 692 Fragments arranged by size: 1306 692 189 BssSI C'ACGA_G Cuts at: 0 205 1597 2187 Size: 205 1392 590 Fragments arranged by size: 1392 590 205 BstEII G'GTnAC_C Cuts at: 0 424 1269 2187 Size: 424 845 918 Fragments arranged by size: 918 845 424 BstXI CCAn_nnnn'nTGG Cuts at: 0 373 534 1050 1107 1599 2077 2142 2187 Size: 373 161 516 57 492 478 65 45 Fragments arranged by size: 516 492 478 373 161 65 57 45 BstYI r'GATC_y Cuts at: 0 1558 1586 1673 1775 2187 Size: 1558 28 87 102 412 Fragments arranged by size: 1558 412 102 87 28 Bsu36I CC'TnA_GG Cuts at: 0 430 2157 2187 Size: 430 1727 30 Fragments arranged by size: 1727 430 30 BtgI C'CryG_G Cuts at: 0 533 1019 1106 1179 1426 1628 1694 1784 Size: 533 486 87 73 247 202 66 90 Cuts at: 1784 2187 Size: 403 Fragments arranged by size: 533 486 403 247 202 90 87 73 66 BtsI GCAGTG_nn' Cuts at: 0 1463 1770 2150 2187 Size: 1463 307 380 37 Fragments arranged by size: 1463 380 307 37 EaeI y'GGCC_r Cuts at: 0 191 1787 2187 Size: 191 1596 400 Fragments arranged by size: 1596 400 191 EarI CTCTTCn'nnn_ Cuts at: 0 1529 2187 Size: 1529 658 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1551 2187 Size: 1551 636 EcoICRI GAG'CTC Cuts at: 0 242 2187 Size: 242 1945 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 816 929 975 1073 1561 2048 2187 Size: 49 767 113 46 98 488 487 139 Fragments arranged by size: 767 488 487 139 113 98 49 46 EcoNI CCTnn'n_nnAGG Cuts at: 0 264 1204 2187 Size: 264 940 983 Fragments arranged by size: 983 940 264 EcoO109I rG'GnC_Cy Cuts at: 0 578 579 861 1224 2187 Size: 578 1 282 363 963 Fragments arranged by size: 963 578 363 282 1 EcoRV GAT'ATC Cuts at: 0 450 654 2187 Size: 450 204 1533 Fragments arranged by size: 1533 450 204 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1540 2187 Size: 1540 647 HaeII r_GCGC'y Cuts at: 0 187 954 2187 Size: 187 767 1233 Fragments arranged by size: 1233 767 187 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 437 469 1202 1234 1271 1303 1341 1373 Size: 437 32 733 32 37 32 38 32 Cuts at: 1373 1578 1610 1696 1728 2187 Size: 205 32 86 32 459 Fragments arranged by size: 733 459 437 205 86 38 37 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1476 2187 Size: 1476 711 KasI G'GCGC_C Cuts at: 0 950 2187 Size: 950 1237 KpnI G_GTAC'C Cuts at: 0 489 2187 Size: 489 1698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 197 2187 Size: 197 1990 MslI CAynn'nnrTG Cuts at: 0 371 661 976 1105 1597 1876 2075 2187 Size: 371 290 315 129 492 279 199 112 Fragments arranged by size: 492 371 315 290 279 199 129 112 MspA1I CmG'CkG Cuts at: 0 512 552 1046 1786 2187 Size: 512 40 494 740 401 Fragments arranged by size: 740 512 494 401 40 NaeI GCC'GGC Cuts at: 0 191 1497 2187 Size: 191 1306 690 Fragments arranged by size: 1306 690 191 NarI GG'CG_CC Cuts at: 0 951 2187 Size: 951 1236 NcoI C'CATG_G Cuts at: 0 1019 1179 1628 2187 Size: 1019 160 449 559 Fragments arranged by size: 1019 559 449 160 NdeI CA'TA_TG Cuts at: 0 956 2187 Size: 956 1231 NgoMIV G'CCGG_C Cuts at: 0 189 1495 2187 Size: 189 1306 692 Fragments arranged by size: 1306 692 189 NspI r_CATG'y Cuts at: 0 1152 2187 Size: 1152 1035 PasI CC'CwG_GG Cuts at: 0 865 1501 2187 Size: 865 636 686 Fragments arranged by size: 865 686 636 PciI A'CATG_T Cuts at: 0 1148 2187 Size: 1148 1039 PflMI CCAn_nnn'nTGG Cuts at: 0 1020 2187 Size: 1020 1167 PfoI T'CCnGG_A Cuts at: 0 244 871 1089 1345 1582 2187 Size: 244 627 218 256 237 605 Fragments arranged by size: 627 605 256 244 237 218 PmlI CAC'GTG Cuts at: 0 1748 2187 Size: 1748 439 PpuMI rG'GwC_Cy Cuts at: 0 1224 2187 Size: 1224 963 PshAI GACnn'nnGTC Cuts at: 0 1518 2187 Size: 1518 669 PspOMI G'GGCC_C Cuts at: 0 578 861 2043 2187 Size: 578 283 1182 144 Fragments arranged by size: 1182 578 283 144 PspXI vC'TCGA_Gb Cuts at: 0 383 2187 Size: 383 1804 PstI C_TGCA'G Cuts at: 0 329 577 1568 2187 Size: 329 248 991 619 Fragments arranged by size: 991 619 329 248 PvuII CAG'CTG Cuts at: 0 552 1046 2187 Size: 552 494 1141 Fragments arranged by size: 1141 552 494 SacI G_AGCT'C Cuts at: 0 244 2187 Size: 244 1943 SacII CC_GC'GG Cuts at: 0 1787 2187 Size: 1787 400 SfcI C'TryA_G Cuts at: 0 109 325 405 573 1564 2187 Size: 109 216 80 168 991 623 Fragments arranged by size: 991 623 216 168 109 80 SfoI GGC'GCC Cuts at: 0 952 2187 Size: 952 1235 SmlI C'TyrA_G Cuts at: 0 383 643 1893 2011 2187 Size: 383 260 1250 118 176 Fragments arranged by size: 1250 383 260 176 118 StuI AGG'CCT Cuts at: 0 270 394 2187 Size: 270 124 1793 Fragments arranged by size: 1793 270 124 StyI C'CwwG_G Cuts at: 0 1019 1179 1265 1401 1628 2039 2129 2187 Size: 1019 160 86 136 227 411 90 58 Fragments arranged by size: 1019 411 227 160 136 90 86 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1027 2187 Size: 1027 1160 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1658 1690 1856 1888 1959 1991 2187 Size: 1658 32 166 32 71 32 196 Fragments arranged by size: 1658 196 166 71 32 32 32 XhoI C'TCGA_G Cuts at: 0 383 2187 Size: 383 1804 ZraI GAC'GTC Cuts at: 0 322 2187 Size: 322 1865 Enzymes that do cut: AatII AccI Acc65I AcuI AflIII AleI AlfI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI KpnI MmeI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII SfcI SfoI SmlI StuI StyI TaqII TstI XhoI ZraI Enzymes that do not cut: AarI AclI AfeI AflII AgeI AhdI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BciVI BfrBI BglII BlpI BmtI BsaBI BsiEI BsiWI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoRI FseI FspI FspAI HindIII HpaI MfeI MluI MscI NheI NotI NruI NsiI PacI PmeI PpiI PsiI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI XmaI XmnI