Restriction map for
pFN21AE9543
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AgeI AhdI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BamHI
BbeI BciVI BfaI BfrI BglI
BlnI BsaBI BsaHI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII KasI KpnI KspI
MaeI MamI MfeI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmeI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 533 TCCGGA T^CCGGA
AflIII 184 ACGTGT A^C[GA][CT]GT
Alw44I 13 GTGCAC G^TGCAC
AlwI 111 GATCC GGATC(4/5)
ApaLI 13 GTGCAC G^TGCAC
AspI 259 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1411 GGCACC G^G[CT][GA]CC
BbrPI 183 CACGTG CAC^GTG
BbsI 607 GTCTTC GAAGAC(2/6)
BbvCI 591 GCTGAGG CCTCAGC(-5/-2)
BcgI 485 GCACATCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 923 TGATCA T^GATCA
BcnI 1139 CCCGG CC^[GC]GG
BglII 318 AGATCT A^GATCT
BlpI 1084 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1084 GCTCAGC GC^T[ACGT]AGC
BpuAI 607 GTCTTC GAAGAC(2/6)
BsaAI 183 CACGTG [CT]AC^GT[GA]
BsaWI 533 TCCGGA [AT]^CCGG[AT]
BseAI 533 TCCGGA T^CCGGA
BseRI 34 CTCCTC GAGGAG(10/8)
BsmBI 1382 CGTCTC CGTCTC(1/5)
BsmI 63 GCATTC GAATGC(1/-1)
BspEI 533 TCCGGA T^CCGGA
BsrDI 1025 GCAATG GCAATG(2/0)
BssSI 1228 CTCGTG CACGAG(-5/-1)
BstAPI 647 GCAACCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1084 GCTCAGC GC^T[ACGT]AGC
DsaI 512 CCATGG C^C[GA][CT]GG
EcoT14I 512 CCATGG C^C[AT][AT]GG
EcoT22I 61 ATGCAT ATGCA^T
FbaI 923 TGATCA T^GATCA
HincII 955 GTTAAC GT[CT]^[GA]AC
HindII 955 GTTAAC GT[CT]^[GA]AC
HpaI 955 GTTAAC GTT^AAC
MroI 533 TCCGGA T^CCGGA
MseI 956 TTAA T^TAA
NciI 1139 CCCGG CC^[GC]GG
NcoI 512 CCATGG C^CATGG
NsiI 61 ATGCAT ATGCA^T
NspI 1454 GCATGT [GA]CATG^[CT]
PflFI 259 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 183 CACGTG CAC^GTG
PmlI 183 CACGTG CAC^GTG
PpuMI 823 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1376 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 235 GAGCTC GAGCT^C
SapI 237 GCTCTTC GCTCTTC(1/4)
SfcI 480 CTACAG C^T[GA][CT]AG
StyI 512 CCATGG C^C[AT][AT]GG
TaqI 737 TCGA T^CGA
Tru9I 956 TTAA T^TAA
Tth111I 259 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 737 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AspHI 13 GTGCAC G[AT]GC[AT]^C
235 GAGCTC
BalI 1388 TGGCCA TGG^CCA
1481 TGGCCA
BsaI 271 GAGACC GGTCTC(1/5)
769 GAGACC
BsiHKAI 13 GTGCAC G[AT]GC[AT]^C
235 GAGCTC
BstXI 95 CCATGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
506 CCACTGCCATGG
BstYI 110 AGATCC [GA]^GATC[CT]
318 AGATCT
EaeI 1388 TGGCCA [CT]^GGCC[GA]
1481 TGGCCA
EarI 238 CTCTTC CTCTTC(1/4)
751 CTCTTC
FauI 196 CCCGC CCCGC(4/6)
1080 GCGGG
Ksp632I 238 CTCTTC CTCTTC(1/4)
751 CTCTTC
MaeII 17 ACGT A^CGT
184 ACGT
MflI 110 AGATCC [GA]^GATC[CT]
318 AGATCT
MluNI 1388 TGGCCA TGG^CCA
1481 TGGCCA
MscI 1388 TGGCCA TGG^CCA
1481 TGGCCA
PvuII 225 CAGCTG CAG^CTG
1047 CAGCTG
XcmI 122 CCAATGAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
256 CCAGACCAAGTCTGG
XhoII 110 AGATCC [GA]^GATC[CT]
318 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI TseI Tsp45I
TspRI