(Circular) (Six-base) MAPSORT of: pFN21AE9543.seq Check: 2480 from: 1 to: 6193 pFN21AE9543 6193 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4003 278 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3536 1937 Size: 1599 4594 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2993 3034 4256 5640 5908 1632 Size: 1361 41 1222 1384 268 1917 Fragments arranged by size: 1917 1384 1361 1222 268 41 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 2189 5092 6069 1203 Size: 986 2903 977 1327 Fragments arranged by size: 2903 1327 986 977 AgeI A'CCGG_T Cuts at: 5024 5024 Size: 6193 AhdI GACnn_n'nnGTC Cuts at: 4922 4922 Size: 6193 AleI CACnn'nnGTG Cuts at: 1166 2709 1166 Size: 1543 4650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6193 AlwNI CAG_nnn'CTG Cuts at: 3936 5508 3936 Size: 1572 4621 ApaLI G'TGCA_C Cuts at: 2018 4250 5406 2018 Size: 2232 1156 2805 Fragments arranged by size: 2805 2232 1156 ApoI r'AATT_y Cuts at: 1493 3502 3728 3880 5989 1493 Size: 2009 226 152 2109 1697 Fragments arranged by size: 2109 2009 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4747 160 Size: 872 3715 1606 Fragments arranged by size: 3715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 3518 1870 Size: 73 1575 4545 Fragments arranged by size: 4545 1575 73 BamHI G'GATC_C Cuts at: 3523 3890 3523 Size: 367 5826 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3416 3514 4969 618 Size: 325 217 627 1629 98 1455 1842 Fragments arranged by size: 1842 1629 1455 627 325 217 98 BanII G_rGCy'C Cuts at: 1391 2244 3091 3257 3512 1391 Size: 853 847 166 255 4072 Fragments arranged by size: 4072 853 847 255 166 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2605 928 Size: 971 706 4516 Fragments arranged by size: 4516 971 706 BbvCI CC'TCA_GC Cuts at: 2597 2597 Size: 6193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2479 2513 4384 4418 1903 Size: 34 542 34 1871 34 3678 Fragments arranged by size: 3678 1871 542 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4085 5295 4085 Size: 1210 4983 BclI T'GATC_A Cuts at: 1548 2928 1548 Size: 1380 4813 BfrBI ATG'CAT Cuts at: 2068 2068 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4804 136 Size: 107 122 71 631 605 3132 1525 Fragments arranged by size: 3132 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2323 6188 2323 Size: 3865 2328 BlpI GC'TnA_GC Cuts at: 3090 3610 3090 Size: 520 5673 Bme1580I G_kGCm'C Cuts at: 1790 2022 3419 4254 5410 1790 Size: 232 1397 835 1156 2573 Fragments arranged by size: 2573 1397 1156 835 232 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6193 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2442 3924 4882 Size: 416 53 83 399 24 1025 1482 958 Cuts at: 4882 442 Size: 1753 Fragments arranged by size: 1753 1482 1025 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2142 3000 4853 1239 Size: 890 13 858 1853 2579 Fragments arranged by size: 2579 1853 890 858 13 Bpu10I CC'TnA_GC Cuts at: 2238 2597 3434 2238 Size: 359 837 4997 Fragments arranged by size: 4997 837 359 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4318 5183 5481 4318 Size: 865 298 5030 Fragments arranged by size: 5030 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2270 2768 4856 882 Size: 617 771 498 2088 2219 Fragments arranged by size: 2219 2088 771 617 498 BsaAI yAC'GTr Cuts at: 493 1204 2190 3984 493 Size: 711 986 1794 2702 Fragments arranged by size: 2702 1794 986 711 BsaBI GATnn'nnATC Cuts at: 6187 6187 Size: 6193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4000 4382 5017 275 Size: 53 83 186 936 2467 382 635 1451 Fragments arranged by size: 2467 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2538 4626 5024 5298 5445 5856 2538 Size: 2088 398 274 147 411 2875 Fragments arranged by size: 2875 2088 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2300 2330 1178 Size: 30 165 30 897 30 5041 Fragments arranged by size: 5041 897 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 1186 Size: 844 5349 BseYI C'CCAG_C Cuts at: 2823 2892 3244 3250 3420 5396 5892 2823 Size: 69 352 6 170 1976 496 3124 Fragments arranged by size: 3124 1976 496 352 170 69 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2341 2531 2536 3020 3080 1163 Size: 573 605 190 5 484 60 4276 Fragments arranged by size: 4276 605 573 484 190 60 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3643 4404 5432 1540 Size: 275 186 1642 761 1028 2301 Fragments arranged by size: 2301 1642 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2022 2244 4254 4339 5410 2022 Size: 222 2010 85 1071 2805 Fragments arranged by size: 2805 2010 1071 222 85 BsmI GAATG_Cn' Cuts at: 2068 3704 3797 2068 Size: 1636 93 4464 Fragments arranged by size: 4464 1636 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3393 3393 Size: 6193 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2244 3091 3257 3419 3512 4254 Size: 399 232 222 847 166 162 93 742 Cuts at: 4254 4339 5410 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 847 742 399 232 222 166 162 93 85 BspEI T'CCGG_A Cuts at: 2538 5856 2538 Size: 3318 2875 BspHI T'CATG_A Cuts at: 4082 5812 4082 Size: 1730 4463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3548 844 Size: 2704 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4080 1394 Size: 429 2257 3507 Fragments arranged by size: 3507 2257 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3037 4688 4862 65 Size: 1183 1789 1651 174 1396 Fragments arranged by size: 1789 1651 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4837 5024 1700 Size: 110 3027 187 2869 Fragments arranged by size: 3027 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6193 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6193 BssSI C'ACGA_G Cuts at: 3233 4253 5265 3233 Size: 1020 1012 4161 Fragments arranged by size: 4161 1020 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2658 794 Size: 1864 4329 BstBI TT'CG_AA Cuts at: 4997 4997 Size: 6193 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6193 BstXI CCAn_nnnn'nTGG Cuts at: 2107 2518 2107 Size: 411 5782 BstYI r'GATC_y Cuts at: 1972 2115 2323 3523 3890 4275 4292 5744 5830 Size: 143 208 1200 367 385 17 1452 86 Cuts at: 5830 5842 6188 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1200 385 367 346 208 143 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2517 513 Size: 552 939 513 4189 Fragments arranged by size: 4189 939 552 513 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5454 Fragments arranged by size: 5454 683 56 BtsI GCAGTG_nn' Cuts at: 2511 2548 2656 3406 3709 4503 4523 2511 Size: 37 108 750 303 794 20 4181 Fragments arranged by size: 4181 794 750 303 108 37 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6158 DraI TTT'AAA Cuts at: 3497 3497 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5200 809 Size: 507 3884 1802 Fragments arranged by size: 3884 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3393 3486 3640 Size: 54 1425 50 275 18 1563 93 154 Cuts at: 3640 4529 6158 8 Size: 889 1629 43 Fragments arranged by size: 1629 1563 1425 889 275 154 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3640 1537 Size: 275 1828 4090 Fragments arranged by size: 4090 1828 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2249 2762 4123 1353 Size: 896 513 1361 3423 Fragments arranged by size: 3423 1361 896 513 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4780 5154 5300 387 Size: 1064 138 3191 374 146 1280 Fragments arranged by size: 3191 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2242 2242 Size: 6193 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2142 2993 3000 3034 4256 4853 Size: 393 497 13 851 7 34 1222 597 Cuts at: 4853 5640 5908 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1222 851 787 597 497 393 268 34 13 7 EcoO109I rG'GnC_Cy Cuts at: 2467 2746 2829 2936 2467 Size: 279 83 107 5724 Fragments arranged by size: 5724 279 107 83 EcoRI G'AATT_C Cuts at: 3502 3880 3502 Size: 378 5815 FspI TGC'GCA Cuts at: 4699 4699 Size: 6193 HaeII r_GCGC'y Cuts at: 1678 5340 1678 Size: 3662 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2300 2332 2357 2389 4834 Size: 32 135 32 728 32 25 32 2445 Cuts at: 4834 4866 4908 4940 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2445 728 135 42 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 2962 3537 3783 669 Size: 988 281 1024 575 246 3079 Fragments arranged by size: 3079 1024 988 575 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6193 HpaI GTT'AAC Cuts at: 2962 3783 2962 Size: 821 5372 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 3792 3792 Size: 6193 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2174 2355 2745 5307 5491 1305 Size: 869 181 390 2562 184 2007 Fragments arranged by size: 2562 2007 869 390 184 181 MscI TGG'CCA Cuts at: 10 64 1489 1832 3395 3488 10 Size: 54 1425 343 1563 93 2715 Fragments arranged by size: 2715 1563 1425 343 93 54 MslI CAynn'nnrTG Cuts at: 518 1166 2211 2339 2516 2570 2709 2996 4151 Size: 648 1045 128 177 54 139 287 1155 Cuts at: 4151 4510 4669 518 Size: 359 159 2042 Fragments arranged by size: 2042 1155 1045 648 359 287 177 159 139 128 54 MspA1I CmG'CkG Cuts at: 1606 2232 3054 3085 3609 4286 5434 5679 1606 Size: 626 822 31 524 677 1148 245 2120 Fragments arranged by size: 2120 1148 822 677 626 524 245 31 NcoI C'CATG_G Cuts at: 513 1065 2004 2517 513 Size: 552 939 513 4189 Fragments arranged by size: 4189 939 552 513 NdeI CA'TA_TG Cuts at: 387 387 Size: 6193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6193 NotI GC'GGCC_GC Cuts at: 3640 3640 Size: 6193 NsiI A_TGCA'T Cuts at: 2070 2070 Size: 6193 NspI r_CATG'y Cuts at: 1132 1229 3463 3551 5096 1132 Size: 97 2234 88 1545 2229 Fragments arranged by size: 2234 2229 1545 97 88 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6193 PciI A'CATG_T Cuts at: 5092 5092 Size: 6193 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6193 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PmlI CAC'GTG Cuts at: 2190 2190 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4262 4294 5775 5807 4262 Size: 32 1481 32 4648 Fragments arranged by size: 4648 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2829 2829 Size: 6193 PshAI GACnn'nnGTC Cuts at: 1613 3385 1613 Size: 1772 4421 PsiI TTA'TAA Cuts at: 3763 3763 Size: 6193 PstI C_TGCA'G Cuts at: 830 3545 830 Size: 2715 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6193 PvuII CAG'CTG Cuts at: 2232 3054 2232 Size: 822 5371 SacI G_AGCT'C Cuts at: 2244 2244 Size: 6193 SalI G'TCGA_C Cuts at: 1936 3535 1936 Size: 1599 4594 SanDI GG'GwC_CC Cuts at: 2829 2829 Size: 6193 SapI GCTCTTCn'nnn_ Cuts at: 2249 2249 Size: 6193 SbfI CC_TGCA'GG Cuts at: 3545 3545 Size: 6193 ScaI AGT'ACT Cuts at: 4441 6153 4441 Size: 1712 4481 SfcI C'TryA_G Cuts at: 826 1045 2485 3541 4676 5357 5548 826 Size: 219 1440 1056 1135 681 191 1471 Fragments arranged by size: 1471 1440 1135 1056 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6193 SmaI CCC'GGG Cuts at: 1872 3520 1872 Size: 1648 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4297 5198 5460 820 Size: 197 926 2354 901 262 1553 Fragments arranged by size: 2354 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SspI AAT'ATT Cuts at: 5 52 4117 5 Size: 47 4065 2081 Fragments arranged by size: 4065 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6193 StyI C'CwwG_G Cuts at: 513 1065 2004 2517 3632 513 Size: 552 939 513 1115 3074 Fragments arranged by size: 3074 1115 939 552 513 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2201 2294 2914 3434 4220 4237 4390 Size: 1255 621 93 620 520 786 17 153 Cuts at: 4390 4575 5020 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 786 621 620 520 445 185 153 93 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4439 6151 96 Size: 274 80 33 51 1443 2462 1712 138 Fragments arranged by size: 2462 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6153 6185 6153 Size: 32 6161 Tth111I GACn'n_nGTC Cuts at: 1316 2267 1316 Size: 951 5242 XbaI T'CTAG_A Cuts at: 3529 3529 Size: 6193 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2134 2268 2134 Size: 134 6059 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6193 XmaI C'CCGG_G Cuts at: 1870 3518 1870 Size: 1648 4545 XmnI GAAnn'nnTTC Cuts at: 4322 4322 Size: 6193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4001 276 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI