Restriction map for
pFN21AE9543
(Linear) (Six-base) MAPSORT of: KIEE9543.seq Check: 6343 from: 1 to: 1488
KIEE9543.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 1029 1488
Size: 988 41 459
Fragments arranged by size:
988 459 41
AflIII A'CryG_T
Cuts at: 0 184 1488
Size: 184 1304
AleI CACnn'nnGTG
Cuts at: 0 704 1488
Size: 704 784
ApaLI G'TGCA_C
Cuts at: 0 13 1488
Size: 13 1475
BanI G'GyrC_C
Cuts at: 0 1411 1488
Size: 1411 77
BanII G_rGCy'C
Cuts at: 0 239 1086 1252 1488
Size: 239 847 166 236
Fragments arranged by size:
847 239 236 166
BbsI GAAGACnn'nnnn_
Cuts at: 0 600 1488
Size: 600 888
BbvCI CC'TCA_GC
Cuts at: 0 592 1488
Size: 592 896
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 474 508 1488
Size: 474 34 980
Fragments arranged by size:
980 474 34
BclI T'GATC_A
Cuts at: 0 923 1488
Size: 923 565
BfrBI ATG'CAT
Cuts at: 0 63 1488
Size: 63 1425
BglII A'GATC_T
Cuts at: 0 318 1488
Size: 318 1170
BlpI GC'TnA_GC
Cuts at: 0 1085 1488
Size: 1085 403
Bme1580I G_kGCm'C
Cuts at: 0 17 1414 1488
Size: 17 1397 74
Fragments arranged by size:
1397 74 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 437 1488
Size: 437 1051
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 137 995 1488
Size: 124 13 858 493
Fragments arranged by size:
858 493 124 13
Bpu10I CC'TnA_GC
Cuts at: 0 233 592 1429 1488
Size: 233 359 837 59
Fragments arranged by size:
837 359 233 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 265 763 1488
Size: 265 498 725
Fragments arranged by size:
725 498 265
BsaAI yAC'GTr
Cuts at: 0 185 1488
Size: 185 1303
BsaWI w'CCGG_w
Cuts at: 0 533 1488
Size: 533 955
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 1488
Size: 295 30 1163
Fragments arranged by size:
1163 295 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 1488
Size: 25 1463
BseYI C'CCAG_C
Cuts at: 0 818 887 1239 1245 1415 1488
Size: 818 69 352 6 170 73
Fragments arranged by size:
818 352 170 73 69 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 526 531 1015 1075 1488
Size: 336 190 5 484 60 413
Fragments arranged by size:
484 413 336 190 60 5
BsiHKAI G_wGCw'C
Cuts at: 0 17 239 1488
Size: 17 222 1249
Fragments arranged by size:
1249 222 17
BsmI GAATG_Cn'
Cuts at: 0 63 1488
Size: 63 1425
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1388 1488
Size: 1388 100
Bsp1286I G_dGCh'C
Cuts at: 0 17 239 1086 1252 1414 1488
Size: 17 222 847 166 162 74
Fragments arranged by size:
847 222 166 162 74 17
BspEI T'CCGG_A
Cuts at: 0 533 1488
Size: 533 955
BsrDI GCAATG_nn'
Cuts at: 0 1032 1488
Size: 1032 456
BssSI C'ACGA_G
Cuts at: 0 1228 1488
Size: 1228 260
BstAPI GCAn_nnn'nTGC
Cuts at: 0 653 1488
Size: 653 835
BstXI CCAn_nnnn'nTGG
Cuts at: 0 102 513 1488
Size: 102 411 975
Fragments arranged by size:
975 411 102
BstYI r'GATC_y
Cuts at: 0 110 318 1488
Size: 110 208 1170
Fragments arranged by size:
1170 208 110
BtgI C'CryG_G
Cuts at: 0 512 1488
Size: 512 976
BtsI GCAGTG_nn'
Cuts at: 0 506 543 651 1401 1488
Size: 506 37 108 750 87
Fragments arranged by size:
750 506 108 87 37
EaeI y'GGCC_r
Cuts at: 0 1388 1481 1488
Size: 1388 93 7
Fragments arranged by size:
1388 93 7
EarI CTCTTCn'nnn_
Cuts at: 0 244 757 1488
Size: 244 513 731
Fragments arranged by size:
731 513 244
EcoICRI GAG'CTC
Cuts at: 0 237 1488
Size: 237 1251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 137 988 995 1029 1488
Size: 124 13 851 7 34 459
Fragments arranged by size:
851 459 124 34 13 7
EcoO109I rG'GnC_Cy
Cuts at: 0 462 741 824 931 1488
Size: 462 279 83 107 557
Fragments arranged by size:
557 462 279 107 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 352 384 1488
Size: 295 32 25 32 1104
Fragments arranged by size:
1104 295 32 32 25
HincII GTy'rAC
Cuts at: 0 957 1488
Size: 957 531
HpaI GTT'AAC
Cuts at: 0 957 1488
Size: 957 531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 350 740 1488
Size: 169 181 390 748
Fragments arranged by size:
748 390 181 169
MscI TGG'CCA
Cuts at: 0 1390 1483 1488
Size: 1390 93 5
Fragments arranged by size:
1390 93 5
MslI CAynn'nnrTG
Cuts at: 0 206 334 511 565 704 991 1488
Size: 206 128 177 54 139 287 497
Fragments arranged by size:
497 287 206 177 139 128 54
MspA1I CmG'CkG
Cuts at: 0 227 1049 1080 1488
Size: 227 822 31 408
Fragments arranged by size:
822 408 227 31
NcoI C'CATG_G
Cuts at: 0 512 1488
Size: 512 976
NsiI A_TGCA'T
Cuts at: 0 65 1488
Size: 65 1423
NspI r_CATG'y
Cuts at: 0 1458 1488
Size: 1458 30
PmlI CAC'GTG
Cuts at: 0 185 1488
Size: 185 1303
PpuMI rG'GwC_Cy
Cuts at: 0 824 1488
Size: 824 664
PshAI GACnn'nnGTC
Cuts at: 0 1380 1488
Size: 1380 108
PvuII CAG'CTG
Cuts at: 0 227 1049 1488
Size: 227 822 439
Fragments arranged by size:
822 439 227
SacI G_AGCT'C
Cuts at: 0 239 1488
Size: 239 1249
SanDI GG'GwC_CC
Cuts at: 0 824 1488
Size: 824 664
SapI GCTCTTCn'nnn_
Cuts at: 0 244 1488
Size: 244 1244
SfcI C'TryA_G
Cuts at: 0 480 1488
Size: 480 1008
StyI C'CwwG_G
Cuts at: 0 512 1488
Size: 512 976
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 196 289 909 1429 1488
Size: 196 93 620 520 59
Fragments arranged by size:
620 520 196 93 59
Tth111I GACn'n_nGTC
Cuts at: 0 262 1488
Size: 262 1226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 129 263 1488
Size: 129 134 1225
Fragments arranged by size:
1225 134 129
Enzymes that do cut:
AcuI AflIII AleI ApaLI BanI BanII BbsI BbvCI
BcgI BclI BfrBI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrDI BssSI BstAPI
BstXI BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI
EcoO109I Hin4I HincII HpaI MmeI MscI MslI MspA1I
NcoI NsiI NspI PmlI PpuMI PshAI PvuII SacI
SanDI SapI SfcI StyI TaqII Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BciVI BglI
BmgBI BmtI BplI BpuEI BsaBI BsaHI BsiEI BsiWI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PpiI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
XbaI XhoI XmaI XmnI ZraI