################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI ScaI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcsI 652 GAATTC [GA]^AATT[CT] Alw44I 1316 GTGCAC G^TGCAC ApaLI 1316 GTGCAC G^TGCAC ApoI 652 GAATTC [GA]^AATT[CT] Asp700I 1380 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 786 GGTGCC G^G[CT][GA]CC BbsI 673 GAAGAC GAAGAC(2/6) BbvCI 1050 CCTCAGC CCTCAGC(-5/-2) BpuAI 673 GAAGAC GAAGAC(2/6) BsaAI 813 CACGTA [CT]AC^GT[GA] BsaI 215 GAGACC GGTCTC(1/5) BsrDI 1194 CATTGC GCAATG(2/0) BsrFI 95 GCCGGC [GA]^CCGG[CT] BstXI 162 CCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 95 GCCGGC [GA]^CCGG[CT] DraIII 362 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 450 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 652 GAATTC G^AATTC HindIII 1402 AAGCTT A^AGCTT MaeIII 324 GTCAC ^GT[ACGT]AC MslI 1194 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 95 GCCGGC GCC^GGC NgoMIV 95 GCCGGC G^CCGGC NspI 1208 GCATGT [GA]CATG^[CT] PpuMI 458 AGGACCT [GA]G^G[AT]CC[CT] SbfI 1065 CCTGCAGG CCTGCA^GG SexAI 1037 ACCTGGT A^CC[AT]GGT SfiI 252 GGCCATGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 1065 CCTGCAGG CCTGCA^GG StuI 1272 AGGCCT AGG^CCT TaqI 1259 TCGA T^CGA Tsp45I 324 GTCAC ^GT[GC]AC Tsp509I 653 AATT ^AATT TthHB8I 1259 TCGA T^CGA XmnI 1380 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 380 ACACGT A^C[GA][CT]GT 926 ACGTGT AlwI 852 GATCC GGATC(4/5) 1100 GGATC ApaI 421 GGGCCC GGGCC^C 465 GGGCCC BfaI 107 CTAG C^TAG 364 CTAG BstYI 851 AGATCC [GA]^GATC[CT] 1100 GGATCT DpnII 852 GATC ^GATC 1101 GATC KspI 1321 CCGCGG CCGC^GG 1392 CCGCGG MaeI 107 CTAG C^TAG 364 CTAG MboI 852 GATC ^GATC 1101 GATC MflI 851 AGATCC [GA]^GATC[CT] 1100 GGATCT MseI 875 TTAA T^TAA 1420 TTAA NcoI 80 CCATGG C^CATGG 1436 CCATGG NdeII 852 GATC ^GATC 1101 GATC PvuII 30 CAGCTG CAG^CTG 1057 CAGCTG SacI 1153 GAGCTC GAGCT^C 1327 GAGCTC SacII 1321 CCGCGG CCGC^GG 1392 CCGCGG Sau3AI 852 GATC ^GATC 1101 GATC SfaNI 801 GATGC GCATC(5/9) 1137 GCATC SmaI 248 CCCGGG CCC^GGG 1350 CCCGGG SmlI 148 CTCAAG C^T[CT][GA]AG 1330 CTCAAG TfiI 645 GATTC G^A[AT]TC 1345 GATTC Tru9I 875 TTAA T^TAA 1420 TTAA TspRI 267 CACTG CA[GC]TG[ACGT][ACGT]^ 1314 CAGTG XhoII 851 AGATCC [GA]^GATC[CT] 1100 GGATCT XmaCI 248 CCCGGG C^CCGGG 1350 CCCGGG XmaI 248 CCCGGG C^CCGGG 1350 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI AvaI AvaII BalI BanII BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PflMI PleI PstI RsaI Sau96I ScrFI SfcI StyI TseI Van91I XcmI