(Circular) (Six-base) MAPSORT of: pFN21AB0445.seq Check: 272 from: 1 to: 6178 pFN21AB0445 6178 bp With 182 enzymes: SgfI * March 28, 2012 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2122 2318 2122 Size: 196 5982 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3988 278 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3521 1937 Size: 1584 4594 Acc65I G'GTAC_C Cuts at: 3499 3499 Size: 6178 AclI AA'CG_TT Cuts at: 1563 4305 4678 1563 Size: 2742 373 3063 Fragments arranged by size: 3063 2742 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2213 3214 4241 5625 5893 1632 Size: 581 1001 1027 1384 268 1917 Fragments arranged by size: 1917 1384 1027 1001 581 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5981 AflIII A'CryG_T Cuts at: 1203 2385 2931 5077 6054 1203 Size: 1182 546 2146 977 1327 Fragments arranged by size: 2146 1327 1182 977 546 AgeI A'CCGG_T Cuts at: 5009 5009 Size: 6178 AhdI GACnn_n'nnGTC Cuts at: 4907 4907 Size: 6178 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6178 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6178 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2768 2800 2768 Size: 32 6146 AlwNI CAG_nnn'CTG Cuts at: 2398 2410 2467 2944 3121 3382 3921 5493 2398 Size: 12 57 477 177 261 539 1572 3083 Fragments arranged by size: 3083 1572 539 477 261 177 57 12 ApaI G_GGCC'C Cuts at: 2430 2474 2430 Size: 44 6134 ApaLI G'TGCA_C Cuts at: 3321 4235 5391 3321 Size: 914 1156 4108 Fragments arranged by size: 4108 1156 914 ApoI r'AATT_y Cuts at: 1493 2657 3487 3713 3865 5974 1493 Size: 1164 830 226 152 2109 1697 Fragments arranged by size: 2109 1697 1164 830 226 152 AseI AT'TA_AT Cuts at: 160 1032 4732 160 Size: 872 3700 1606 Fragments arranged by size: 3700 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 AvaI C'yCGr_G Cuts at: 1870 1943 2253 2743 3355 3503 1870 Size: 73 310 490 612 148 4545 Fragments arranged by size: 4545 612 490 310 148 73 BamHI G'GATC_C Cuts at: 3508 3875 3508 Size: 367 5811 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2791 3499 4954 618 Size: 325 217 627 1004 708 1455 1842 Fragments arranged by size: 1842 1455 1004 708 627 325 217 BanII G_rGCy'C Cuts at: 1391 2025 2094 2430 2474 3162 3336 3497 1391 Size: 634 69 336 44 688 174 161 4072 Fragments arranged by size: 4072 688 634 336 174 161 69 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2685 928 Size: 971 786 4421 Fragments arranged by size: 4421 971 786 BbvCI CC'TCA_GC Cuts at: 3056 3056 Size: 6178 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4369 4403 1903 Size: 34 2432 34 3678 Fragments arranged by size: 3678 2432 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4070 5280 4070 Size: 1210 4968 BclI T'GATC_A Cuts at: 1548 1548 Size: 6178 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 2264 3066 Size: 107 122 71 631 605 380 212 802 Cuts at: 3066 3387 4789 136 Size: 321 1402 1525 Fragments arranged by size: 1525 1402 802 631 605 380 321 212 122 107 71 BglII A'GATC_T Cuts at: 6173 6173 Size: 6178 BlpI GC'TnA_GC Cuts at: 2033 2447 3161 3595 2033 Size: 414 714 434 4616 Fragments arranged by size: 4616 714 434 414 Bme1580I G_kGCm'C Cuts at: 1790 2430 2474 2796 3325 3438 4239 5395 1790 Size: 640 44 322 529 113 801 1156 2573 Fragments arranged by size: 2573 1156 801 640 529 322 113 44 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6178 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3312 3454 3909 Size: 416 53 83 399 24 1895 142 455 Cuts at: 3909 4867 442 Size: 958 1753 Fragments arranged by size: 1895 1753 958 455 416 399 142 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6178 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2746 2864 3176 3359 4838 1239 Size: 1507 118 312 183 1479 2579 Fragments arranged by size: 2579 1507 1479 312 183 118 Bpu10I CC'TnA_GC Cuts at: 2422 3015 3056 2422 Size: 593 41 5544 Fragments arranged by size: 5544 593 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2138 3320 4303 5168 5466 2138 Size: 1182 983 865 298 2850 Fragments arranged by size: 2850 1182 983 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2214 4841 882 Size: 617 715 2627 2219 Fragments arranged by size: 2627 2219 715 617 BsaAI yAC'GTr Cuts at: 493 1204 2820 3969 493 Size: 711 1616 1149 2702 Fragments arranged by size: 2702 1616 1149 711 BsaBI GATnn'nnATC Cuts at: 6172 6172 Size: 6178 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3985 4367 5002 275 Size: 53 83 186 936 2452 382 635 1451 Fragments arranged by size: 2452 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4611 5009 5283 5430 5841 4611 Size: 398 274 147 411 4948 Fragments arranged by size: 4948 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2133 2150 2163 2270 Size: 30 165 30 717 13 17 13 107 Cuts at: 2270 2300 1178 Size: 30 5056 Fragments arranged by size: 5056 717 165 107 30 30 30 17 13 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 2687 3461 1186 Size: 955 546 774 3903 Fragments arranged by size: 3903 955 774 546 BseYI C'CCAG_C Cuts at: 3064 5381 5877 3064 Size: 2317 496 3365 Fragments arranged by size: 3365 2317 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2396 2954 3158 1163 Size: 573 660 558 204 4183 Fragments arranged by size: 4183 660 573 558 204 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3628 4389 5417 1540 Size: 275 186 1627 761 1028 2301 Fragments arranged by size: 2301 1627 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 2377 3162 3325 3336 4239 4324 5395 2034 Size: 343 785 163 11 903 85 1071 2817 Fragments arranged by size: 2817 1071 903 785 343 163 85 11 BsmI GAATG_Cn' Cuts at: 3689 3782 3689 Size: 93 6085 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2034 2094 2377 2430 2474 2796 Size: 399 235 9 60 283 53 44 322 Cuts at: 2796 3162 3325 3336 3438 3497 4239 4324 5395 Size: 366 163 11 102 59 742 85 1071 Cuts at: 5395 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 366 322 283 235 163 102 85 60 59 53 44 11 9 BspEI T'CCGG_A Cuts at: 5841 5841 Size: 6178 BspHI T'CATG_A Cuts at: 4067 5797 4067 Size: 1730 4448 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2122 2318 2514 3105 3533 844 Size: 1278 196 196 591 428 3489 Fragments arranged by size: 3489 1278 591 428 196 196 BsrBI CCG'CTC Cuts at: 1394 1823 4065 1394 Size: 429 2242 3507 Fragments arranged by size: 3507 2242 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3198 4673 4847 65 Size: 1183 1950 1475 174 1396 Fragments arranged by size: 1950 1475 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2100 4822 5009 1700 Size: 110 290 2722 187 2869 Fragments arranged by size: 2869 2722 290 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6178 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6178 BssSI C'ACGA_G Cuts at: 4238 5250 4238 Size: 1012 5166 BstAPI GCAn_nnn'nTGC Cuts at: 794 2372 2398 2407 794 Size: 1578 26 9 4565 Fragments arranged by size: 4565 1578 26 9 BstBI TT'CG_AA Cuts at: 4982 4982 Size: 6178 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6178 BstXI CCAn_nnnn'nTGG Cuts at: 2174 2174 Size: 6178 BstYI r'GATC_y Cuts at: 1972 2856 3105 3508 3875 4260 4277 5729 5815 Size: 884 249 403 367 385 17 1452 86 Cuts at: 5815 5827 6173 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 884 403 385 367 346 249 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2085 2637 2796 3326 3397 3441 Size: 552 939 81 552 159 530 71 44 Cuts at: 3441 513 Size: 3250 Fragments arranged by size: 3250 939 552 552 530 159 81 71 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5439 Fragments arranged by size: 5439 683 56 BtsI GCAGTG_nn' Cuts at: 2271 3694 4488 4508 2271 Size: 1423 794 20 3941 Fragments arranged by size: 3941 1423 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6178 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2902 2937 3181 3216 529 Size: 35 2338 35 244 35 3491 Fragments arranged by size: 3491 2338 244 35 35 35 DraI TTT'AAA Cuts at: 3482 3482 Size: 6178 DraIII CAC_nnn'GTG Cuts at: 2372 2372 Size: 6178 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5185 809 Size: 507 3869 1802 Fragments arranged by size: 3869 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2179 2946 3048 Size: 54 1425 50 275 18 349 767 102 Cuts at: 3048 3189 3625 4514 6143 8 Size: 141 436 889 1629 43 Fragments arranged by size: 1629 1425 889 767 436 349 275 141 102 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3625 1537 Size: 275 1813 4090 Fragments arranged by size: 4090 1813 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2189 3257 3293 4108 1353 Size: 836 1068 36 815 3423 Fragments arranged by size: 3423 1068 836 815 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4765 5139 5285 387 Size: 1064 138 3176 374 146 1280 Fragments arranged by size: 3176 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3160 3334 3160 Size: 174 6004 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2746 2864 3176 3214 3359 4241 Size: 393 581 533 118 312 38 145 882 Cuts at: 4241 4838 5625 5893 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 882 787 597 581 533 393 312 268 145 118 38 EcoNI CCTnn'n_nnAGG Cuts at: 2459 2459 Size: 6178 EcoO109I rG'GnC_Cy Cuts at: 2054 2426 2427 2464 2471 3088 2054 Size: 372 1 37 7 617 5144 Fragments arranged by size: 5144 617 372 37 7 1 EcoRI G'AATT_C Cuts at: 2657 3487 3865 2657 Size: 830 378 4970 Fragments arranged by size: 4970 830 378 FspI TGC'GCA Cuts at: 4684 4684 Size: 6178 HaeII r_GCGC'y Cuts at: 1678 2012 2085 2354 5325 1678 Size: 334 73 269 2971 2531 Fragments arranged by size: 2971 2531 334 269 73 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 2270 2302 2508 Size: 32 135 32 548 32 118 32 206 Cuts at: 2508 2540 4819 4851 4893 4925 1373 Size: 32 2279 32 42 32 2626 Fragments arranged by size: 2626 2279 548 206 135 118 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3522 3768 669 Size: 988 281 1584 246 3079 Fragments arranged by size: 3079 1584 988 281 246 HindIII A'AGCT_T Cuts at: 748 3407 748 Size: 2659 3519 HpaI GTT'AAC Cuts at: 3768 3768 Size: 6178 KpnI G_GTAC'C Cuts at: 3503 3503 Size: 6178 MfeI C'AATT_G Cuts at: 3777 3777 Size: 6178 MluI A'CGCG_T Cuts at: 6054 6054 Size: 6178 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2509 5292 5476 1305 Size: 1204 2783 184 2007 Fragments arranged by size: 2783 2007 1204 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2181 2948 3050 10 Size: 54 1425 343 349 767 102 3138 Fragments arranged by size: 3138 1425 767 349 343 102 54 MslI CAynn'nnrTG Cuts at: 518 1166 3203 4136 4495 4654 518 Size: 648 2037 933 359 159 2042 Fragments arranged by size: 2042 2037 933 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2037 2827 3064 3328 3399 3594 4271 5419 Size: 431 790 237 264 71 195 677 1148 Cuts at: 5419 5664 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 790 677 431 264 245 237 195 71 NaeI GCC'GGC Cuts at: 2102 2102 Size: 6178 NcoI C'CATG_G Cuts at: 513 1065 2004 2085 3441 513 Size: 552 939 81 1356 3250 Fragments arranged by size: 3250 1356 939 552 81 NdeI CA'TA_TG Cuts at: 387 387 Size: 6178 NgoMIV G'CCGG_C Cuts at: 2100 2100 Size: 6178 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6178 NotI GC'GGCC_GC Cuts at: 3625 3625 Size: 6178 NspI r_CATG'y Cuts at: 1132 1229 3217 3536 5081 1132 Size: 97 1988 319 1545 2229 Fragments arranged by size: 2229 1988 1545 319 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6178 PciI A'CATG_T Cuts at: 5077 5077 Size: 6178 PflMI CCAn_nnn'nTGG Cuts at: 1108 2248 2467 2944 1108 Size: 1140 219 477 4342 Fragments arranged by size: 4342 1140 477 219 PfoI T'CCnGG_A Cuts at: 2538 2538 Size: 6178 PmeI GTTT'AAAC Cuts at: 3482 3482 Size: 6178 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4247 4279 5760 5792 4247 Size: 32 1481 32 4633 Fragments arranged by size: 4633 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2464 2464 Size: 6178 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6178 PsiI TTA'TAA Cuts at: 3748 3748 Size: 6178 PspOMI G'GGCC_C Cuts at: 2426 2470 2426 Size: 44 6134 PstI C_TGCA'G Cuts at: 830 2216 2511 3075 3530 830 Size: 1386 295 564 455 3478 Fragments arranged by size: 3478 1386 564 455 295 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6178 PvuII CAG'CTG Cuts at: 2037 3064 2037 Size: 1027 5151 SacI G_AGCT'C Cuts at: 3162 3336 3162 Size: 174 6004 SacII CC_GC'GG Cuts at: 3329 3400 3329 Size: 71 6107 SalI G'TCGA_C Cuts at: 1936 3520 1936 Size: 1584 4594 SbfI CC_TGCA'GG Cuts at: 3075 3530 3075 Size: 455 5723 ScaI AGT'ACT Cuts at: 4426 6138 4426 Size: 1712 4466 SexAI A'CCwGG_T Cuts at: 3042 3042 Size: 6178 SfcI C'TryA_G Cuts at: 826 1045 2212 2507 3071 3526 4661 5342 5533 Size: 219 1167 295 564 455 1135 681 191 Cuts at: 5533 826 Size: 1471 Fragments arranged by size: 1471 1167 1135 681 564 455 295 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2264 2264 Size: 6178 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6178 SmaI CCC'GGG Cuts at: 1872 2255 3357 3505 1872 Size: 383 1102 148 4545 Fragments arranged by size: 4545 1102 383 148 SmlI C'TyrA_G Cuts at: 820 1017 1943 2153 3335 4282 5183 5445 820 Size: 197 926 210 1182 947 901 262 1553 Fragments arranged by size: 1553 1182 947 926 901 262 210 197 SnaBI TAC'GTA Cuts at: 493 3969 493 Size: 3476 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6178 SphI G_CATG'C Cuts at: 1132 3536 1132 Size: 2404 3774 SrfI GCCC'GGGC Cuts at: 2255 2255 Size: 6178 SspI AAT'ATT Cuts at: 5 52 4102 5 Size: 47 4050 2081 Fragments arranged by size: 4050 2081 47 StuI AGG'CCT Cuts at: 1517 3279 1517 Size: 1762 4416 StyI C'CwwG_G Cuts at: 513 1065 2004 2085 2094 3000 3441 3474 3617 Size: 552 939 81 9 906 441 33 143 Cuts at: 3617 513 Size: 3074 Fragments arranged by size: 3074 939 906 552 441 143 81 33 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2614 2930 2988 4205 4222 4375 4560 Size: 1255 1034 316 58 1217 17 153 185 Cuts at: 4560 5005 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1217 1034 445 316 185 153 58 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4424 6136 96 Size: 274 80 33 51 1443 2447 1712 138 Fragments arranged by size: 2447 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2133 2165 3265 3297 6138 6170 2133 Size: 32 1100 32 2841 32 2141 Fragments arranged by size: 2841 2141 1100 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6178 XbaI T'CTAG_A Cuts at: 3514 3514 Size: 6178 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2092 2174 2982 2092 Size: 82 808 5288 Fragments arranged by size: 5288 808 82 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6178 XmaI C'CCGG_G Cuts at: 1870 2253 3355 3503 1870 Size: 383 1102 148 4545 Fragments arranged by size: 4545 1102 383 148 XmnI GAAnn'nnTTC Cuts at: 3389 4307 3389 Size: 918 5260 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3986 276 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SfoI SwaI