(Linear) (Six-base) MAPSORT of: KIBB0445.seq Check: 4064 from: 1 to: 1473 KIBB0445.orf With 182 enzymes: SgfI * March 22, 2012 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 117 313 1473 Size: 117 196 1160 Fragments arranged by size: 1160 196 117 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 1209 1473 Size: 208 1001 264 Fragments arranged by size: 1001 264 208 AflIII A'CryG_T Cuts at: 0 380 926 1473 Size: 380 546 547 Fragments arranged by size: 547 546 380 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 763 795 1473 Size: 763 32 678 Fragments arranged by size: 763 678 32 AlwNI CAG_nnn'CTG Cuts at: 0 393 405 462 939 1116 1377 1473 Size: 393 12 57 477 177 261 96 Fragments arranged by size: 477 393 261 177 96 57 12 ApaI G_GGCC'C Cuts at: 0 425 469 1473 Size: 425 44 1004 Fragments arranged by size: 1004 425 44 ApaLI G'TGCA_C Cuts at: 0 1316 1473 Size: 1316 157 ApoI r'AATT_y Cuts at: 0 652 1473 Size: 652 821 AvaI C'yCGr_G Cuts at: 0 248 738 1350 1473 Size: 248 490 612 123 Fragments arranged by size: 612 490 248 123 BanI G'GyrC_C Cuts at: 0 786 1473 Size: 786 687 BanII G_rGCy'C Cuts at: 0 20 89 425 469 1157 1331 1473 Size: 20 69 336 44 688 174 142 Fragments arranged by size: 688 336 174 142 69 44 20 BbsI GAAGACnn'nnnn_ Cuts at: 0 680 1473 Size: 680 793 BbvCI CC'TCA_GC Cuts at: 0 1051 1473 Size: 1051 422 BglI GCCn_nnn'nGGC Cuts at: 0 47 259 1061 1382 1473 Size: 47 212 802 321 91 Fragments arranged by size: 802 321 212 91 47 BlpI GC'TnA_GC Cuts at: 0 28 442 1156 1473 Size: 28 414 714 317 Fragments arranged by size: 714 414 317 28 Bme1580I G_kGCm'C Cuts at: 0 425 469 791 1320 1433 1473 Size: 425 44 322 529 113 40 Fragments arranged by size: 529 425 322 113 44 40 BmrI ACTGGGnnnn_n' Cuts at: 0 1307 1449 1473 Size: 1307 142 24 Fragments arranged by size: 1307 142 24 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 741 859 1171 1354 1473 Size: 741 118 312 183 119 Fragments arranged by size: 741 312 183 119 118 Bpu10I CC'TnA_GC Cuts at: 0 417 1010 1051 1473 Size: 417 593 41 422 Fragments arranged by size: 593 422 417 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 1315 1473 Size: 133 1182 158 Fragments arranged by size: 1182 158 133 BsaI GGTCTCn'nnnn_ Cuts at: 0 209 1473 Size: 209 1264 BsaAI yAC'GTr Cuts at: 0 815 1473 Size: 815 658 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 115 128 145 158 265 295 1473 Size: 115 13 17 13 107 30 1178 Fragments arranged by size: 1178 115 107 30 17 13 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 136 682 1456 1473 Size: 136 546 774 17 Fragments arranged by size: 774 546 136 17 BseYI C'CCAG_C Cuts at: 0 1059 1473 Size: 1059 414 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 391 949 1153 1473 Size: 391 558 204 320 Fragments arranged by size: 558 391 320 204 BsiHKAI G_wGCw'C Cuts at: 0 29 372 1157 1320 1331 1473 Size: 29 343 785 163 11 142 Fragments arranged by size: 785 343 163 142 29 11 Bsp1286I G_dGCh'C Cuts at: 0 20 29 89 372 425 469 791 1157 Size: 20 9 60 283 53 44 322 366 Cuts at: 1157 1320 1331 1433 1473 Size: 163 11 102 40 Fragments arranged by size: 366 322 283 163 102 60 53 44 40 20 11 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 117 313 509 1100 1473 Size: 117 196 196 591 373 Fragments arranged by size: 591 373 196 196 117 BsrDI GCAATG_nn' Cuts at: 0 1193 1473 Size: 1193 280 BsrFI r'CCGG_y Cuts at: 0 95 1473 Size: 95 1378 BstAPI GCAn_nnn'nTGC Cuts at: 0 367 393 402 1473 Size: 367 26 9 1071 Fragments arranged by size: 1071 367 26 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 169 1473 Size: 169 1304 BstYI r'GATC_y Cuts at: 0 851 1100 1473 Size: 851 249 373 Fragments arranged by size: 851 373 249 BtgI C'CryG_G Cuts at: 0 80 632 791 1321 1392 1436 1473 Size: 80 552 159 530 71 44 37 Fragments arranged by size: 552 530 159 80 71 44 37 BtsI GCAGTG_nn' Cuts at: 0 266 1473 Size: 266 1207 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 897 932 1176 1211 1473 Size: 897 35 244 35 262 Fragments arranged by size: 897 262 244 35 35 DraIII CAC_nnn'GTG Cuts at: 0 367 1473 Size: 367 1106 EaeI y'GGCC_r Cuts at: 0 174 941 1043 1184 1473 Size: 174 767 102 141 289 Fragments arranged by size: 767 289 174 141 102 EarI CTCTTCn'nnn_ Cuts at: 0 184 1252 1288 1473 Size: 184 1068 36 185 Fragments arranged by size: 1068 185 184 36 EcoICRI GAG'CTC Cuts at: 0 1155 1329 1473 Size: 1155 174 144 Fragments arranged by size: 1155 174 144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 741 859 1171 1209 1354 1473 Size: 208 533 118 312 38 145 119 Fragments arranged by size: 533 312 208 145 119 118 38 EcoNI CCTnn'n_nnAGG Cuts at: 0 454 1473 Size: 454 1019 EcoO109I rG'GnC_Cy Cuts at: 0 49 421 422 459 466 1083 1473 Size: 49 372 1 37 7 617 390 Fragments arranged by size: 617 390 372 49 37 7 1 EcoRI G'AATT_C Cuts at: 0 652 1473 Size: 652 821 HaeII r_GCGC'y Cuts at: 0 7 80 349 1473 Size: 7 73 269 1124 Fragments arranged by size: 1124 269 73 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 115 147 265 297 503 535 1473 Size: 115 32 118 32 206 32 938 Fragments arranged by size: 938 206 118 115 32 32 32 HindIII A'AGCT_T Cuts at: 0 1402 1473 Size: 1402 71 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 504 1473 Size: 504 969 MscI TGG'CCA Cuts at: 0 176 943 1045 1473 Size: 176 767 102 428 Fragments arranged by size: 767 428 176 102 MslI CAynn'nnrTG Cuts at: 0 1198 1473 Size: 1198 275 MspA1I CmG'CkG Cuts at: 0 32 822 1059 1323 1394 1473 Size: 32 790 237 264 71 79 Fragments arranged by size: 790 264 237 79 71 32 NaeI GCC'GGC Cuts at: 0 97 1473 Size: 97 1376 NcoI C'CATG_G Cuts at: 0 80 1436 1473 Size: 80 1356 37 Fragments arranged by size: 1356 80 37 NgoMIV G'CCGG_C Cuts at: 0 95 1473 Size: 95 1378 NspI r_CATG'y Cuts at: 0 1212 1473 Size: 1212 261 PflMI CCAn_nnn'nTGG Cuts at: 0 243 462 939 1473 Size: 243 219 477 534 Fragments arranged by size: 534 477 243 219 PfoI T'CCnGG_A Cuts at: 0 533 1473 Size: 533 940 PpuMI rG'GwC_Cy Cuts at: 0 459 1473 Size: 459 1014 PspOMI G'GGCC_C Cuts at: 0 421 465 1473 Size: 421 44 1008 Fragments arranged by size: 1008 421 44 PstI C_TGCA'G Cuts at: 0 211 506 1070 1473 Size: 211 295 564 403 Fragments arranged by size: 564 403 295 211 PvuII CAG'CTG Cuts at: 0 32 1059 1473 Size: 32 1027 414 Fragments arranged by size: 1027 414 32 SacI G_AGCT'C Cuts at: 0 1157 1331 1473 Size: 1157 174 142 Fragments arranged by size: 1157 174 142 SacII CC_GC'GG Cuts at: 0 1324 1395 1473 Size: 1324 71 78 Fragments arranged by size: 1324 78 71 SbfI CC_TGCA'GG Cuts at: 0 1070 1473 Size: 1070 403 SexAI A'CCwGG_T Cuts at: 0 1037 1473 Size: 1037 436 SfcI C'TryA_G Cuts at: 0 207 502 1066 1473 Size: 207 295 564 407 Fragments arranged by size: 564 407 295 207 SfiI GGCCn_nnn'nGGCC Cuts at: 0 259 1473 Size: 259 1214 SmaI CCC'GGG Cuts at: 0 250 1352 1473 Size: 250 1102 121 Fragments arranged by size: 1102 250 121 SmlI C'TyrA_G Cuts at: 0 148 1330 1473 Size: 148 1182 143 Fragments arranged by size: 1182 148 143 SrfI GCCC'GGGC Cuts at: 0 250 1473 Size: 250 1223 StuI AGG'CCT Cuts at: 0 1274 1473 Size: 1274 199 StyI C'CwwG_G Cuts at: 0 80 89 995 1436 1473 Size: 80 9 906 441 37 Fragments arranged by size: 906 441 80 37 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 609 925 983 1473 Size: 609 316 58 490 Fragments arranged by size: 609 490 316 58 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 128 160 1260 1292 1473 Size: 128 32 1100 32 181 Fragments arranged by size: 1100 181 128 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 87 169 977 1473 Size: 87 82 808 496 Fragments arranged by size: 808 496 87 82 XmaI C'CCGG_G Cuts at: 0 248 1350 1473 Size: 248 1102 123 Fragments arranged by size: 1102 248 123 XmnI GAAnn'nnTTC Cuts at: 0 1384 1473 Size: 1384 89 Enzymes that do cut: AarI AcuI AflIII AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SbfI SexAI SfcI SfiI SmaI SmlI SrfI StuI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI EciI EcoRV FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI ScaI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TatI Tth111I XbaI XhoI ZraI