(Circular) (Six-base) MAPSORT of: pFN21AB5183.seq Check: 2422 from: 1 to: 6007 pFN21AB5183 6007 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3817 278 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2568 3350 1937 Size: 631 782 4594 Fragments arranged by size: 4594 782 631 Acc65I G'GTAC_C Cuts at: 3328 3328 Size: 6007 AclI AA'CG_TT Cuts at: 1563 4134 4507 1563 Size: 2571 373 3063 Fragments arranged by size: 3063 2571 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2213 3078 4070 5454 5722 1632 Size: 581 865 992 1384 268 1917 Fragments arranged by size: 1917 1384 992 865 581 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 4906 5883 1203 Size: 3703 977 1327 Fragments arranged by size: 3703 1327 977 AgeI A'CCGG_T Cuts at: 4838 4838 Size: 6007 AhdI GACnn_n'nnGTC Cuts at: 2788 4736 2788 Size: 1948 4059 AleI CACnn'nnGTG Cuts at: 1166 2621 1166 Size: 1455 4552 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3038 1184 Size: 1854 4153 AlwNI CAG_nnn'CTG Cuts at: 3750 5322 3750 Size: 1572 4435 ApaI G_GGCC'C Cuts at: 2029 2029 Size: 6007 ApaLI G'TGCA_C Cuts at: 3169 4064 5220 3169 Size: 895 1156 3956 Fragments arranged by size: 3956 1156 895 ApoI r'AATT_y Cuts at: 1493 3316 3542 3694 5803 1493 Size: 1823 226 152 2109 1697 Fragments arranged by size: 2109 1823 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4561 160 Size: 872 3529 1606 Fragments arranged by size: 3529 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 2028 3332 1870 Size: 73 85 1304 4545 Fragments arranged by size: 4545 1304 85 73 BamHI G'GATC_C Cuts at: 2940 3337 3704 2940 Size: 397 367 5243 Fragments arranged by size: 5243 397 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3191 3328 4783 618 Size: 325 217 627 1404 137 1455 1842 Fragments arranged by size: 1842 1455 1404 627 325 217 137 BanII G_rGCy'C Cuts at: 1391 2029 3326 1391 Size: 638 1297 4072 Fragments arranged by size: 4072 1297 638 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5036 BbvCI CC'TCA_GC Cuts at: 2150 2150 Size: 6007 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4198 4232 1903 Size: 34 2261 34 3678 Fragments arranged by size: 3678 2261 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3899 5109 3899 Size: 1210 4797 BclI T'GATC_A Cuts at: 1548 2460 1548 Size: 912 5095 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4618 136 Size: 107 122 71 631 605 2946 1525 Fragments arranged by size: 2946 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2019 6002 2019 Size: 3983 2024 BlpI GC'TnA_GC Cuts at: 2376 2409 3424 2376 Size: 33 1015 4959 Fragments arranged by size: 4959 1015 33 Bme1580I G_kGCm'C Cuts at: 1790 2029 2112 2839 3173 3196 4068 5224 1790 Size: 239 83 727 334 23 872 1156 2573 Fragments arranged by size: 2573 1156 872 727 334 239 83 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2106 2513 2829 Size: 416 53 83 399 24 689 407 316 Cuts at: 2829 3291 3738 4696 442 Size: 462 447 958 1753 Fragments arranged by size: 1753 958 689 462 447 416 407 399 316 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 2309 2678 4667 1239 Size: 926 144 369 1989 2579 Fragments arranged by size: 2579 1989 926 369 144 Bpu10I CC'TnA_GC Cuts at: 2150 2242 3121 2150 Size: 92 879 5036 Fragments arranged by size: 5036 879 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2564 3014 4132 4997 5295 2564 Size: 450 1118 865 298 3276 Fragments arranged by size: 3276 1118 865 450 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2252 2878 4670 882 Size: 617 753 626 1792 2219 Fragments arranged by size: 2219 1792 753 626 617 BsaAI yAC'GTr Cuts at: 493 1204 3798 493 Size: 711 2594 2702 Fragments arranged by size: 2702 2594 711 BsaBI GATnn'nnATC Cuts at: 6001 6001 Size: 6007 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3814 4196 4831 275 Size: 53 83 186 936 2281 382 635 1451 Fragments arranged by size: 2281 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4440 4838 5112 5259 5670 4440 Size: 398 274 147 411 4777 Fragments arranged by size: 4777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2198 2228 1178 Size: 30 165 30 795 30 4957 Fragments arranged by size: 4957 795 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6007 BseYI C'CCAG_C Cuts at: 2830 3096 3253 5210 5706 2830 Size: 266 157 1957 496 3131 Fragments arranged by size: 3131 1957 496 266 157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2580 1163 Size: 573 844 4590 Fragments arranged by size: 4590 844 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3457 4218 5246 1540 Size: 275 186 1456 761 1028 2301 Fragments arranged by size: 2301 1456 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3173 4068 4153 5224 3173 Size: 895 85 1071 3956 Fragments arranged by size: 3956 1071 895 85 BsmI GAATG_Cn' Cuts at: 3518 3611 3518 Size: 93 5914 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2112 2839 3173 3196 3326 4068 Size: 399 239 83 727 334 23 130 742 Cuts at: 4068 4153 5224 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 727 399 334 239 130 85 83 23 BspEI T'CCGG_A Cuts at: 5670 5670 Size: 6007 BspHI T'CATG_A Cuts at: 2508 3234 3896 5626 2508 Size: 726 662 1730 2889 Fragments arranged by size: 2889 1730 726 662 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3362 844 Size: 2518 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2380 2992 3894 1394 Size: 429 557 612 902 3507 Fragments arranged by size: 3507 902 612 557 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2403 3144 4502 4676 65 Size: 1183 1155 741 1358 174 1396 Fragments arranged by size: 1396 1358 1183 1155 741 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2446 4651 4838 1700 Size: 110 636 2205 187 2869 Fragments arranged by size: 2869 2205 636 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6007 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6007 BssSI C'ACGA_G Cuts at: 4067 5079 4067 Size: 1012 4995 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6007 BstBI TT'CG_AA Cuts at: 4811 4811 Size: 6007 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6007 BstXI CCAn_nnnn'nTGG Cuts at: 2623 2656 3092 3304 2623 Size: 33 436 212 5326 Fragments arranged by size: 5326 436 212 33 BstYI r'GATC_y Cuts at: 1972 2019 2034 2071 2940 3337 3704 4089 4106 Size: 47 15 37 869 397 367 385 17 Cuts at: 4106 5558 5644 5656 6002 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 869 397 385 367 346 86 47 37 17 15 12 BtgI C'CryG_G Cuts at: 513 1065 2047 513 Size: 552 982 4473 Fragments arranged by size: 4473 982 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2206 3101 487 Size: 683 56 980 895 3393 Fragments arranged by size: 3393 980 895 683 56 BtsI GCAGTG_nn' Cuts at: 2112 2205 3003 3523 4317 4337 2112 Size: 93 798 520 794 20 3782 Fragments arranged by size: 3782 798 794 520 93 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5972 DraI TTT'AAA Cuts at: 3311 3311 Size: 6007 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5014 809 Size: 507 3698 1802 Fragments arranged by size: 3698 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2352 2415 2493 Size: 54 1425 50 275 18 522 63 78 Cuts at: 2493 3454 4343 5972 8 Size: 961 889 1629 43 Fragments arranged by size: 1629 1425 961 889 522 275 78 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3454 1537 Size: 275 1642 4090 Fragments arranged by size: 4090 1642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3243 3937 1353 Size: 1890 694 3423 Fragments arranged by size: 3423 1890 694 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4594 4968 5114 387 Size: 1064 138 3005 374 146 1280 Fragments arranged by size: 3005 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2213 2309 2678 3078 4070 4667 Size: 393 533 48 96 369 400 992 597 Cuts at: 4667 5454 5722 1239 Size: 787 268 1524 Fragments arranged by size: 1524 992 787 597 533 400 393 369 268 96 48 EcoO109I rG'GnC_Cy Cuts at: 2140 2884 2140 Size: 744 5263 EcoRI G'AATT_C Cuts at: 3316 3694 3316 Size: 378 5629 FspI TGC'GCA Cuts at: 4513 4513 Size: 6007 HaeII r_GCGC'y Cuts at: 1678 5154 1678 Size: 3476 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2297 2329 2774 2806 4648 Size: 32 135 32 725 32 445 32 1842 Cuts at: 4648 4680 4722 4754 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1842 725 445 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3037 3351 3597 669 Size: 988 281 1099 314 246 3079 Fragments arranged by size: 3079 1099 988 314 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6007 HpaI GTT'AAC Cuts at: 3597 3597 Size: 6007 KpnI G_GTAC'C Cuts at: 3332 3332 Size: 6007 MfeI C'AATT_G Cuts at: 3606 3606 Size: 6007 MluI A'CGCG_T Cuts at: 5883 5883 Size: 6007 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2274 5121 5305 1305 Size: 969 2847 184 2007 Fragments arranged by size: 2847 2007 969 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2495 10 Size: 54 1425 343 663 3522 Fragments arranged by size: 3522 1425 663 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2621 3965 4324 4483 518 Size: 648 1455 1344 359 159 2042 Fragments arranged by size: 2042 1455 1344 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2049 2375 2413 2830 3423 4100 5248 5493 Size: 443 326 38 417 593 677 1148 245 Cuts at: 5493 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 593 443 417 326 245 38 NaeI GCC'GGC Cuts at: 2448 2448 Size: 6007 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5455 NdeI CA'TA_TG Cuts at: 387 387 Size: 6007 NgoMIV G'CCGG_C Cuts at: 2446 2446 Size: 6007 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 3454 3454 Size: 6007 NspI r_CATG'y Cuts at: 1132 1229 3365 4910 1132 Size: 97 2136 1545 2229 Fragments arranged by size: 2229 2136 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6007 PciI A'CATG_T Cuts at: 4906 4906 Size: 6007 PflMI CCAn_nnn'nTGG Cuts at: 1108 2950 1108 Size: 1842 4165 PfoI T'CCnGG_A Cuts at: 2037 2655 2037 Size: 618 5389 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4076 4108 5589 5621 4076 Size: 32 1481 32 4462 Fragments arranged by size: 4462 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2884 2884 Size: 6007 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6007 PsiI TTA'TAA Cuts at: 3577 3577 Size: 6007 PspOMI G'GGCC_C Cuts at: 2025 2025 Size: 6007 PstI C_TGCA'G Cuts at: 830 2107 3359 830 Size: 1277 1252 3478 Fragments arranged by size: 3478 1277 1252 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 PvuII CAG'CTG Cuts at: 2375 2413 2830 2375 Size: 38 417 5552 Fragments arranged by size: 5552 417 38 SacII CC_GC'GG Cuts at: 2050 2050 Size: 6007 SalI G'TCGA_C Cuts at: 1936 3349 1936 Size: 1413 4594 SapI GCTCTTCn'nnn_ Cuts at: 3243 3243 Size: 6007 SbfI CC_TGCA'GG Cuts at: 3359 3359 Size: 6007 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SexAI A'CCwGG_T Cuts at: 3153 3153 Size: 6007 SfcI C'TryA_G Cuts at: 826 1045 2103 3355 4490 5171 5362 826 Size: 219 1058 1252 1135 681 191 1471 Fragments arranged by size: 1471 1252 1135 1058 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6007 SmaI CCC'GGG Cuts at: 1872 2030 3334 1872 Size: 158 1304 4545 Fragments arranged by size: 4545 1304 158 SmlI C'TyrA_G Cuts at: 820 1017 1943 2579 3029 4111 5012 5274 820 Size: 197 926 636 450 1082 901 262 1553 Fragments arranged by size: 1553 1082 926 901 636 450 262 197 SnaBI TAC'GTA Cuts at: 493 3798 493 Size: 3305 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 3365 1132 Size: 2233 3774 SspI AAT'ATT Cuts at: 5 52 3931 5 Size: 47 3879 2081 Fragments arranged by size: 3879 2081 47 StuI AGG'CCT Cuts at: 1517 3011 1517 Size: 1494 4513 StyI C'CwwG_G Cuts at: 513 1065 2673 3446 513 Size: 552 1608 773 3074 Fragments arranged by size: 3074 1608 773 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2330 2942 3040 4034 4051 4204 4389 Size: 1255 750 612 98 994 17 153 185 Cuts at: 4389 4834 325 Size: 445 1498 Fragments arranged by size: 1498 1255 994 750 612 445 185 153 98 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4253 5965 96 Size: 274 80 33 51 1443 2276 1712 138 Fragments arranged by size: 2276 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2198 2230 5967 5999 2198 Size: 32 3737 32 2206 Fragments arranged by size: 3737 2206 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2852 1316 Size: 1536 4471 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2065 2335 3185 2065 Size: 270 850 4887 Fragments arranged by size: 4887 850 270 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6007 XmaI C'CCGG_G Cuts at: 1870 2028 3332 1870 Size: 158 1304 4545 Fragments arranged by size: 4545 1304 158 XmnI GAAnn'nnTTC Cuts at: 4136 4136 Size: 6007 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3815 276 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SfiI SfoI SrfI SwaI