(Circular) (Six-base) MAPSORT of: pFN21AB5179.seq Check: 8256 from: 1 to: 5890 pFN21AB5179 5890 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3700 278 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2874 3233 1937 Size: 937 359 4594 Fragments arranged by size: 4594 937 359 Acc65I G'GTAC_C Cuts at: 3011 3211 3011 Size: 200 5690 AclI AA'CG_TT Cuts at: 1563 4017 4390 1563 Size: 2454 373 3063 Fragments arranged by size: 3063 2454 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2126 3122 3953 5337 5605 1632 Size: 494 996 831 1384 268 1917 Fragments arranged by size: 1917 1384 996 831 494 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5693 AflIII A'CryG_T Cuts at: 1203 4789 5766 1203 Size: 3586 977 1327 Fragments arranged by size: 3586 1327 977 AgeI A'CCGG_T Cuts at: 4721 4721 Size: 5890 AhdI GACnn_n'nnGTC Cuts at: 4619 4619 Size: 5890 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5890 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5890 AlwNI CAG_nnn'CTG Cuts at: 3633 5205 3633 Size: 1572 4318 ApaLI G'TGCA_C Cuts at: 3947 5103 3947 Size: 1156 4734 ApoI r'AATT_y Cuts at: 1493 3058 3199 3425 3577 5686 1493 Size: 1565 141 226 152 2109 1697 Fragments arranged by size: 2109 1697 1565 226 152 141 AseI AT'TA_AT Cuts at: 160 1032 4444 160 Size: 872 3412 1606 Fragments arranged by size: 3412 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 AvaI C'yCGr_G Cuts at: 1870 1943 3215 1870 Size: 73 1272 4545 Fragments arranged by size: 4545 1272 73 BamHI G'GATC_C Cuts at: 2149 3220 3587 2149 Size: 1071 367 4452 Fragments arranged by size: 4452 1071 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2843 3011 3211 4666 618 Size: 325 217 627 1056 168 200 1455 1842 Fragments arranged by size: 1842 1455 1056 627 325 217 200 168 BanII G_rGCy'C Cuts at: 1391 3209 1391 Size: 1818 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2394 928 Size: 971 495 4424 Fragments arranged by size: 4424 971 495 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4081 4115 1903 Size: 34 2144 34 3678 Fragments arranged by size: 3678 2144 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2713 3782 4992 2713 Size: 1069 1210 3611 Fragments arranged by size: 3611 1210 1069 BclI T'GATC_A Cuts at: 1548 1548 Size: 5890 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2495 2514 4501 Size: 107 122 71 631 605 823 19 1987 Cuts at: 4501 136 Size: 1525 Fragments arranged by size: 1987 1525 823 631 605 122 107 71 19 BglII A'GATC_T Cuts at: 5885 5885 Size: 5890 BlpI GC'TnA_GC Cuts at: 3307 3307 Size: 5890 Bme1580I G_kGCm'C Cuts at: 1790 2054 3174 3951 5107 1790 Size: 264 1120 777 1156 2573 Fragments arranged by size: 2573 1156 1120 777 264 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5890 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3621 4579 442 Size: 416 53 83 399 24 2204 958 1753 Fragments arranged by size: 2204 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2421 4550 1239 Size: 1182 2129 2579 Fragments arranged by size: 2579 2129 1182 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2343 2535 4015 4880 5178 2343 Size: 192 1480 865 298 3055 Fragments arranged by size: 3055 1480 865 298 192 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2397 2962 4553 882 Size: 617 898 565 1591 2219 Fragments arranged by size: 2219 1591 898 617 565 BsaAI yAC'GTr Cuts at: 493 1204 3681 493 Size: 711 2477 2702 Fragments arranged by size: 2702 2477 711 BsaBI GATnn'nnATC Cuts at: 3096 5884 3096 Size: 2788 3102 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3697 4079 4714 275 Size: 53 83 186 936 2164 382 635 1451 Fragments arranged by size: 2164 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4323 4721 4995 5142 5553 4323 Size: 398 274 147 411 4660 Fragments arranged by size: 4660 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2327 2357 1178 Size: 30 165 30 924 30 4711 Fragments arranged by size: 4711 924 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2459 2853 1186 Size: 874 399 394 4223 Fragments arranged by size: 4223 874 399 394 BseYI C'CCAG_C Cuts at: 2207 5093 5589 2207 Size: 2886 496 2508 Fragments arranged by size: 2886 2508 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2582 1163 Size: 573 846 4471 Fragments arranged by size: 4471 846 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 2878 3340 4101 5129 1540 Size: 275 186 78 799 462 761 1028 2301 Fragments arranged by size: 2301 1028 799 761 462 275 186 78 BsiHKAI G_wGCw'C Cuts at: 2833 3951 4036 5107 2833 Size: 1118 85 1071 3616 Fragments arranged by size: 3616 1118 1071 85 BsmI GAATG_Cn' Cuts at: 3401 3494 3401 Size: 93 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2833 3174 3209 3951 4036 5107 Size: 399 264 779 341 35 742 85 1071 Cuts at: 5107 1391 Size: 2174 Fragments arranged by size: 2174 1071 779 742 399 341 264 85 35 BspEI T'CCGG_A Cuts at: 5553 5553 Size: 5890 BspHI T'CATG_A Cuts at: 3779 5509 3779 Size: 1730 4160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3245 844 Size: 2401 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3777 1394 Size: 429 1954 3507 Fragments arranged by size: 3507 1954 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4385 4559 65 Size: 1183 3137 174 1396 Fragments arranged by size: 3137 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4534 4721 1700 Size: 110 2724 187 2869 Fragments arranged by size: 2869 2724 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5890 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5890 BssSI C'ACGA_G Cuts at: 2030 3950 4962 2030 Size: 1920 1012 2958 Fragments arranged by size: 2958 1920 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2569 794 Size: 1775 4115 BstBI TT'CG_AA Cuts at: 4694 4694 Size: 5890 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5890 BstXI CCAn_nnnn'nTGG Cuts at: 2470 2470 Size: 5890 BstYI r'GATC_y Cuts at: 1972 2149 3091 3097 3220 3587 3972 3989 5441 Size: 177 942 6 123 367 385 17 1452 Cuts at: 5441 5527 5539 5885 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 942 385 367 346 177 123 86 17 12 6 Bsu36I CC'TnA_GG Cuts at: 2769 2769 Size: 5890 BtgI C'CryG_G Cuts at: 513 1065 3161 513 Size: 552 2096 3242 Fragments arranged by size: 3242 2096 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5151 Fragments arranged by size: 5151 683 56 BtsI GCAGTG_nn' Cuts at: 3406 4200 4220 3406 Size: 794 20 5076 Fragments arranged by size: 5076 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5855 DraI TTT'AAA Cuts at: 3194 3194 Size: 5890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4897 809 Size: 507 3581 1802 Fragments arranged by size: 3581 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3136 3337 4226 Size: 54 1425 50 275 18 1306 201 889 Cuts at: 4226 5855 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1306 889 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3337 1537 Size: 275 1525 4090 Fragments arranged by size: 4090 1525 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2631 3017 3820 1353 Size: 1278 386 803 3423 Fragments arranged by size: 3423 1278 803 386 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3059 4477 4851 4997 387 Size: 1064 138 1470 1418 374 146 1280 Fragments arranged by size: 1470 1418 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2421 3122 3953 4550 5337 5605 Size: 393 494 295 701 831 597 787 268 Cuts at: 5605 1239 Size: 1524 Fragments arranged by size: 1524 831 787 701 597 494 393 295 268 EcoNI CCTnn'n_nnAGG Cuts at: 2144 2714 2144 Size: 570 5320 EcoRI G'AATT_C Cuts at: 3199 3577 3199 Size: 378 5512 FspI TGC'GCA Cuts at: 4396 4396 Size: 5890 HaeII r_GCGC'y Cuts at: 1678 5037 1678 Size: 3359 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2327 2359 2784 2816 4531 Size: 32 135 32 755 32 425 32 1715 Cuts at: 4531 4563 4605 4637 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1715 755 425 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2236 2875 3234 3480 669 Size: 988 281 298 639 359 246 3079 Fragments arranged by size: 3079 988 639 359 298 281 246 HindIII A'AGCT_T Cuts at: 748 3179 748 Size: 2431 3459 HpaI GTT'AAC Cuts at: 3480 3480 Size: 5890 KpnI G_GTAC'C Cuts at: 3015 3215 3015 Size: 200 5690 MfeI C'AATT_G Cuts at: 2610 3489 2610 Size: 879 5011 MluI A'CGCG_T Cuts at: 5766 5766 Size: 5890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5004 5188 1305 Size: 3699 184 2007 Fragments arranged by size: 3699 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3138 10 Size: 54 1425 343 1306 2762 Fragments arranged by size: 2762 1425 1306 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3848 4207 4366 518 Size: 648 2682 359 159 2042 Fragments arranged by size: 2682 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2885 3306 3983 5131 5376 1606 Size: 1279 421 677 1148 245 2120 Fragments arranged by size: 2120 1279 1148 677 421 245 NcoI C'CATG_G Cuts at: 513 1065 3161 513 Size: 552 2096 3242 Fragments arranged by size: 3242 2096 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5890 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5890 NotI GC'GGCC_GC Cuts at: 3337 3337 Size: 5890 NspI r_CATG'y Cuts at: 1132 1229 3248 4793 1132 Size: 97 2019 1545 2229 Fragments arranged by size: 2229 2019 1545 97 PasI CC'CwG_GG Cuts at: 1348 3074 1348 Size: 1726 4164 PciI A'CATG_T Cuts at: 4789 4789 Size: 5890 PflMI CCAn_nnn'nTGG Cuts at: 1108 2248 1108 Size: 1140 4750 PmeI GTTT'AAAC Cuts at: 3194 3194 Size: 5890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3959 3991 5472 5504 3959 Size: 32 1481 32 4345 Fragments arranged by size: 4345 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5890 PsiI TTA'TAA Cuts at: 3460 3460 Size: 5890 PstI C_TGCA'G Cuts at: 830 3130 3242 830 Size: 2300 112 3478 Fragments arranged by size: 3478 2300 112 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5890 PvuII CAG'CTG Cuts at: 2885 2885 Size: 5890 SalI G'TCGA_C Cuts at: 1936 2873 3232 1936 Size: 937 359 4594 Fragments arranged by size: 4594 937 359 SbfI CC_TGCA'GG Cuts at: 3242 3242 Size: 5890 ScaI AGT'ACT Cuts at: 4138 5850 4138 Size: 1712 4178 SfcI C'TryA_G Cuts at: 826 1045 2735 3126 3238 4373 5054 5245 826 Size: 219 1690 391 112 1135 681 191 1471 Fragments arranged by size: 1690 1471 1135 681 391 219 191 112 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5890 SmaI CCC'GGG Cuts at: 1872 3217 1872 Size: 1345 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2358 2550 3994 4895 5157 820 Size: 197 926 415 192 1444 901 262 1553 Fragments arranged by size: 1553 1444 926 901 415 262 197 192 SnaBI TAC'GTA Cuts at: 493 3681 493 Size: 3188 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5890 SphI G_CATG'C Cuts at: 1132 3248 1132 Size: 2116 3774 SspI AAT'ATT Cuts at: 5 52 3814 5 Size: 47 3762 2081 Fragments arranged by size: 3762 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5890 StyI C'CwwG_G Cuts at: 513 1065 2331 2525 2868 3161 3329 513 Size: 552 1266 194 343 293 168 3074 Fragments arranged by size: 3074 1266 552 343 293 194 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2065 2106 2893 3917 3934 4087 4272 Size: 1255 485 41 787 1024 17 153 185 Cuts at: 4272 4717 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1024 787 485 445 185 153 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4136 5848 96 Size: 274 80 33 51 1443 2159 1712 138 Fragments arranged by size: 2159 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5850 5882 5850 Size: 32 5858 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5890 XbaI T'CTAG_A Cuts at: 3226 3226 Size: 5890 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5890 XmaI C'CCGG_G Cuts at: 1870 3215 1870 Size: 1345 4545 XmnI GAAnn'nnTTC Cuts at: 3059 4019 3059 Size: 960 4930 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3698 276 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI