Restriction map for
pFN21AB5178
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglII BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccI 1323 GTCTAC GT^[AC][GT]AC
AccII 1429 CGCG CG^CG
Asp700I 864 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 528 TGCGCA TGC^GCA
BclI 1395 TGATCA T^GATCA
BglI 547 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 963 CTGGAG CTGGAG(16/14)
BsaI 1072 GGTCTC GGTCTC(1/5)
BsrFI 37 GCCGGT [GA]^CCGG[CT]
BstAPI 1176 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1429 CGCG CG^CG
BstYI 1162 GGATCT [GA]^GATC[CT]
Cfr10I 37 GCCGGT [GA]^CCGG[CT]
EcoNI 1056 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1395 TGATCA T^GATCA
FspI 528 TGCGCA TGC^GCA
MaeII 842 ACGT A^CGT
MflI 1162 GGATCT [GA]^GATC[CT]
MseI 1337 TTAA T^TAA
MvnI 1429 CGCG CG^CG
NcoI 384 CCATGG C^CATGG
NspI 356 GCATGC [GA]CATG^[CT]
SmlI 995 CTCAAG C^T[CT][GA]AG
SphI 356 GCATGC GCATG^C
StuI 645 AGGCCT AGG^CCT
Tru9I 1337 TTAA T^TAA
XcmI 1139 CCATGCCCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1162 GGATCT [GA]^GATC[CT]
XmnI 864 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
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AlwI 1032 GATCC GGATC(4/5)
1162 GGATC
AocI 48 CCTGAGG CC^T[ACGT]AGG
1345 CCTGAGG
AspHI 11 GAGCAC G[AT]GC[AT]^C
1446 GAGCAC
BalI 326 TGGCCA TGG^CCA
1433 TGGCCA
BanI 421 GGCACC G^G[CT][GA]CC
949 GGCACC
BanII 886 GAGCCC G[GA]GC[CT]^C
1312 GAGCCC
BbsI 292 GAAGAC GAAGAC(2/6)
783 GAAGAC
BfaI 268 CTAG C^TAG
571 CTAG
BlpI 1195 GCTGAGC GC^T[ACGT]AGC
1443 GCTGAGC
Bpu1102I 1195 GCTGAGC GC^T[ACGT]AGC
1443 GCTGAGC
BpuAI 292 GAAGAC GAAGAC(2/6)
783 GAAGAC
BsiHKAI 11 GAGCAC G[AT]GC[AT]^C
1446 GAGCAC
BsmAI 1073 GTCTC GTCTC(1/5)
1116 GTCTC
Bsp1407I 107 TGTACA T^GTACA
558 TGTACA
BsrDI 310 GCAATG GCAATG(2/0)
1244 GCAATG
BsrGI 107 TGTACA T^GTACA
558 TGTACA
BstEII 97 GGTGACC G^GT[ACGT]ACC
1359 GGTGACC
BstPI 97 GGTGACC G^GT[ACGT]ACC
1359 GGTGACC
BstXI 329 CCAACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
617 CCACGGTGGTGG
Bsu36I 48 CCTGAGG CC^T[ACGT]AGG
1345 CCTGAGG
CelII 1195 GCTGAGC GC^T[ACGT]AGC
1443 GCTGAGC
DsaI 384 CCATGG C^C[GA][CT]GG
617 CCACGG
Eco81I 48 CCTGAGG CC^T[ACGT]AGG
1345 CCTGAGG
EcoO65I 97 GGTGACC G^GT[ACGT]ACC
1359 GGTGACC
FauI 1204 CCCGC CCCGC(4/6)
1440 CCCGC
HaeII 1015 AGCGCC [GA]GCGC^[CT]
1402 AGCGCC
HinfI 993 GACTC G^A[ACGT]TC
1321 GAGTC
MaeI 268 CTAG C^TAG
571 CTAG
MluNI 326 TGGCCA TGG^CCA
1433 TGGCCA
MscI 326 TGGCCA TGG^CCA
1433 TGGCCA
PleI 993 GACTC GAGTC(4/5)
1321 GAGTC
PpuMI 536 AGGTCCT [GA]G^G[AT]CC[CT]
1178 AGGACCT
SbfI 549 CCTGCAGG CCTGCA^GG
1173 CCTGCAGG
Sse8387I 549 CCTGCAGG CCTGCA^GG
1173 CCTGCAGG
SspBI 107 TGTACA T^GTACA
558 TGTACA
Tsp509I 213 AATT ^AATT
1238 AATT
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbvI BcnI BmyI BsaJI BsgI
BsiYI BslI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I TspRI