(Circular) (Six-base) MAPSORT of: pFN21AB5178.seq Check: 1530 from: 1 to: 6160 pFN21AB5178 6160 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2090 2090 Size: 6160 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3970 278 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 3503 1937 Size: 1392 174 4594 Fragments arranged by size: 4594 1392 174 Acc65I G'GTAC_C Cuts at: 3481 3481 Size: 6160 AclI AA'CG_TT Cuts at: 1563 4287 4660 1563 Size: 2724 373 3063 Fragments arranged by size: 3063 2724 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2681 4223 5607 5875 1632 Size: 1049 1542 1384 268 1917 Fragments arranged by size: 1917 1542 1384 1049 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5963 AflIII A'CryG_T Cuts at: 1203 5059 6036 1203 Size: 3856 977 1327 Fragments arranged by size: 3856 1327 977 AgeI A'CCGG_T Cuts at: 4991 4991 Size: 6160 AhdI GACnn_n'nnGTC Cuts at: 4889 4889 Size: 6160 AleI CACnn'nnGTG Cuts at: 1166 2627 2924 2970 1166 Size: 1461 297 46 4356 Fragments arranged by size: 4356 1461 297 46 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6160 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2504 2536 2504 Size: 32 6128 AlwNI CAG_nnn'CTG Cuts at: 2572 2614 2683 2930 3187 3903 5475 2572 Size: 42 69 247 257 716 1572 3257 Fragments arranged by size: 3257 1572 716 257 247 69 42 ApaLI G'TGCA_C Cuts at: 4217 5373 4217 Size: 1156 5004 ApoI r'AATT_y Cuts at: 1493 3469 3695 3847 5956 1493 Size: 1976 226 152 2109 1697 Fragments arranged by size: 2109 1976 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4714 160 Size: 872 3682 1606 Fragments arranged by size: 3682 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 AvaI C'yCGr_G Cuts at: 1870 1943 3485 1870 Size: 73 1542 4545 Fragments arranged by size: 4545 1542 73 BamHI G'GATC_C Cuts at: 3490 3857 3490 Size: 367 5793 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2426 2954 3481 4936 618 Size: 325 217 627 639 528 527 1455 1842 Fragments arranged by size: 1842 1455 639 627 528 527 325 217 BanII G_rGCy'C Cuts at: 1391 2895 3321 3479 1391 Size: 1504 426 158 4072 Fragments arranged by size: 4072 1504 426 158 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2304 2795 928 Size: 971 405 491 4293 Fragments arranged by size: 4293 971 491 405 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4351 4385 1903 Size: 34 50 34 2330 34 3678 Fragments arranged by size: 3678 2330 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4052 5262 4052 Size: 1210 4950 BclI T'GATC_A Cuts at: 1548 3400 1548 Size: 1852 4308 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2558 4771 136 Size: 107 122 71 631 605 886 2213 1525 Fragments arranged by size: 2213 1525 886 631 605 122 107 71 BglII A'GATC_T Cuts at: 6155 6155 Size: 6160 BlpI GC'TnA_GC Cuts at: 3201 3449 3577 3201 Size: 248 128 5784 Fragments arranged by size: 5784 248 128 Bme1580I G_kGCm'C Cuts at: 1790 2957 3009 4221 5377 1790 Size: 1167 52 1212 1156 2573 Fragments arranged by size: 2573 1212 1167 1156 52 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6160 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2017 3891 4849 Size: 416 53 83 399 24 600 1874 958 Cuts at: 4849 442 Size: 1753 Fragments arranged by size: 1874 1753 958 600 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2989 4820 1239 Size: 1750 1831 2579 Fragments arranged by size: 2579 1831 1750 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2985 4285 5150 5448 2985 Size: 1300 865 298 3697 Fragments arranged by size: 3697 1300 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3083 4823 882 Size: 617 1584 1740 2219 Fragments arranged by size: 2219 1740 1584 617 BsaAI yAC'GTr Cuts at: 493 1204 3951 493 Size: 711 2747 2702 Fragments arranged by size: 2747 2702 711 BsaBI GATnn'nnATC Cuts at: 6154 6154 Size: 6160 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3967 4349 4984 275 Size: 53 83 186 936 2434 382 635 1451 Fragments arranged by size: 2434 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4593 4991 5265 5412 5823 4593 Size: 398 274 147 411 4930 Fragments arranged by size: 4930 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2504 2534 2802 2832 1178 Size: 30 165 30 1101 30 268 30 4506 Fragments arranged by size: 4506 1101 268 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6160 BseYI C'CCAG_C Cuts at: 2607 5363 5859 2607 Size: 2756 496 2908 Fragments arranged by size: 2908 2756 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2500 2639 2696 3203 3404 1163 Size: 573 764 139 57 507 201 3919 Fragments arranged by size: 3919 764 573 507 201 139 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3610 4371 5399 1540 Size: 275 186 1609 761 1028 2301 Fragments arranged by size: 2301 1609 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2020 3455 4221 4306 5377 2020 Size: 1435 766 85 1071 2803 Fragments arranged by size: 2803 1435 1071 766 85 BsmI GAATG_Cn' Cuts at: 3671 3764 3671 Size: 93 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2895 2957 3009 3321 3455 3479 Size: 399 230 875 62 52 312 134 24 Cuts at: 3479 4221 4306 5377 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 875 742 399 312 230 134 85 62 52 24 BspEI T'CCGG_A Cuts at: 5823 5823 Size: 6160 BspHI T'CATG_A Cuts at: 4049 5779 4049 Size: 1730 4430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2090 2218 3186 3195 3243 3515 844 Size: 1246 128 968 9 48 272 3489 Fragments arranged by size: 3489 1246 968 272 128 48 9 BsrBI CCG'CTC Cuts at: 1394 1823 4047 1394 Size: 429 2224 3507 Fragments arranged by size: 3507 2224 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2322 3256 4655 4829 65 Size: 1183 1074 934 1399 174 1396 Fragments arranged by size: 1399 1396 1183 1074 934 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2042 4804 4991 1700 Size: 110 232 2762 187 2869 Fragments arranged by size: 2869 2762 232 187 110 BsrGI T'GTAC_A Cuts at: 96 2112 2563 96 Size: 2016 451 3693 Fragments arranged by size: 3693 2016 451 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6160 BssSI C'ACGA_G Cuts at: 4220 5232 4220 Size: 1012 5148 BstAPI GCAn_nnn'nTGC Cuts at: 794 3187 794 Size: 2393 3767 BstBI TT'CG_AA Cuts at: 4964 4964 Size: 6160 BstEII G'GTnAC_C Cuts at: 1184 2102 3364 1184 Size: 918 1262 3980 Fragments arranged by size: 3980 1262 918 BstXI CCAn_nnnn'nTGG Cuts at: 2341 2629 2341 Size: 288 5872 BstYI r'GATC_y Cuts at: 1972 3167 3490 3857 4242 4259 5711 5797 5809 Size: 1195 323 367 385 17 1452 86 12 Cuts at: 5809 6155 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1195 385 367 346 323 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2054 3351 2054 Size: 1297 4863 BtgI C'CryG_G Cuts at: 513 1065 2389 2622 513 Size: 552 1324 233 4051 Fragments arranged by size: 4051 1324 552 233 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5421 Fragments arranged by size: 5421 683 56 BtsI GCAGTG_nn' Cuts at: 3411 3676 4470 4490 3411 Size: 265 794 20 5081 Fragments arranged by size: 5081 794 265 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6160 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6125 DraI TTT'AAA Cuts at: 3464 3464 Size: 6160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5167 809 Size: 507 3851 1802 Fragments arranged by size: 3851 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2331 2886 3438 Size: 54 1425 50 275 18 501 555 552 Cuts at: 3438 3607 4496 6125 8 Size: 169 889 1629 43 Fragments arranged by size: 1629 1425 889 555 552 501 275 169 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3607 1537 Size: 275 1795 4090 Fragments arranged by size: 4090 1795 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4090 1353 Size: 2737 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4747 5121 5267 387 Size: 1064 138 3158 374 146 1280 Fragments arranged by size: 3158 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2681 2989 4223 4820 5607 5875 1239 Size: 393 1049 308 1234 597 787 268 1524 Fragments arranged by size: 1524 1234 1049 787 597 393 308 268 EcoNI CCTnn'n_nnAGG Cuts at: 3065 3065 Size: 6160 EcoO109I rG'GnC_Cy Cuts at: 2251 2542 2569 2680 3184 2251 Size: 291 27 111 504 5227 Fragments arranged by size: 5227 504 291 111 27 EcoRI G'AATT_C Cuts at: 3469 3847 3469 Size: 378 5782 FspI TGC'GCA Cuts at: 2535 4666 2535 Size: 2131 4029 FspAI rTGC'GCAy Cuts at: 2535 2535 Size: 6160 HaeII r_GCGC'y Cuts at: 1678 3024 3411 5307 1678 Size: 1346 387 1896 2531 Fragments arranged by size: 2531 1896 1346 387 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2975 3007 3028 3060 4801 Size: 32 135 32 1403 32 21 32 1741 Cuts at: 4801 4833 4875 4907 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1741 1403 135 42 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 669 1657 1938 3504 3750 669 Size: 988 281 1566 246 3079 Fragments arranged by size: 3079 1566 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6160 HpaI GTT'AAC Cuts at: 3750 3750 Size: 6160 KpnI G_GTAC'C Cuts at: 3485 3485 Size: 6160 MfeI C'AATT_G Cuts at: 3759 3759 Size: 6160 MluI A'CGCG_T Cuts at: 6036 6036 Size: 6160 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5274 5458 1305 Size: 3969 184 2007 Fragments arranged by size: 3969 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2333 3440 10 Size: 54 1425 343 501 1107 2730 Fragments arranged by size: 2730 1425 1107 501 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2627 2924 2970 3263 4118 4477 4636 Size: 648 1461 297 46 293 855 359 159 Cuts at: 4636 518 Size: 2042 Fragments arranged by size: 2042 1461 855 648 359 297 293 159 46 MspA1I CmG'CkG Cuts at: 1606 2164 2266 2468 2611 3448 3576 4253 5401 Size: 558 102 202 143 837 128 677 1148 Cuts at: 5401 5646 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 837 677 558 245 202 143 128 102 NcoI C'CATG_G Cuts at: 513 1065 2389 513 Size: 552 1324 4284 Fragments arranged by size: 4284 1324 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6160 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6160 NotI GC'GGCC_GC Cuts at: 3607 3607 Size: 6160 NspI r_CATG'y Cuts at: 1132 1229 2365 3518 5063 1132 Size: 97 1136 1153 1545 2229 Fragments arranged by size: 2229 1545 1153 1136 97 PasI CC'CwG_GG Cuts at: 1348 2584 1348 Size: 1236 4924 PciI A'CATG_T Cuts at: 5059 5059 Size: 6160 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6160 PfoI T'CCnGG_A Cuts at: 2979 2979 Size: 6160 PmeI GTTT'AAAC Cuts at: 3464 3464 Size: 6160 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2504 2536 4229 4261 5742 5774 2504 Size: 32 1693 32 1481 32 2890 Fragments arranged by size: 2890 1693 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2542 3184 2542 Size: 642 5518 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6160 PsiI TTA'TAA Cuts at: 3730 3730 Size: 6160 PstI C_TGCA'G Cuts at: 830 2101 2559 3183 3512 830 Size: 1271 458 624 329 3478 Fragments arranged by size: 3478 1271 624 458 329 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6160 PvuII CAG'CTG Cuts at: 2164 2266 2468 2611 2164 Size: 102 202 143 5713 Fragments arranged by size: 5713 202 143 102 SalI G'TCGA_C Cuts at: 1936 3502 1936 Size: 1566 4594 SbfI CC_TGCA'GG Cuts at: 2559 3183 3512 2559 Size: 624 329 5207 Fragments arranged by size: 5207 624 329 ScaI AGT'ACT Cuts at: 4408 6120 4408 Size: 1712 4448 SfcI C'TryA_G Cuts at: 826 1045 2097 2255 2477 2555 3179 3508 4643 Size: 219 1052 158 222 78 624 329 1135 Cuts at: 4643 5324 5515 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1052 681 624 329 222 219 191 158 78 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6160 SmaI CCC'GGG Cuts at: 1872 3487 1872 Size: 1615 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3000 4264 5165 5427 820 Size: 197 926 1057 1264 901 262 1553 Fragments arranged by size: 1553 1264 1057 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3951 493 Size: 3458 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6160 SphI G_CATG'C Cuts at: 1132 2365 3518 1132 Size: 1233 1153 3774 Fragments arranged by size: 3774 1233 1153 SspI AAT'ATT Cuts at: 5 52 4084 5 Size: 47 4032 2081 Fragments arranged by size: 4032 2081 47 StuI AGG'CCT Cuts at: 1517 2652 1517 Size: 1135 5025 StyI C'CwwG_G Cuts at: 513 1065 2389 2454 2498 2857 3599 513 Size: 552 1324 65 44 359 742 3074 Fragments arranged by size: 3074 1324 742 552 359 65 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2480 2896 4187 4204 4357 4542 4987 Size: 1255 900 416 1291 17 153 185 445 Cuts at: 4987 325 Size: 1498 Fragments arranged by size: 1498 1291 1255 900 445 416 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 2563 4406 Size: 274 80 33 51 1443 135 451 1843 Cuts at: 4406 6118 96 Size: 1712 138 Fragments arranged by size: 1843 1712 1443 451 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2802 2834 6120 6152 2802 Size: 32 3286 32 2810 Fragments arranged by size: 3286 2810 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6160 XbaI T'CTAG_A Cuts at: 3496 3496 Size: 6160 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3151 3151 Size: 6160 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6160 XmaI C'CCGG_G Cuts at: 1870 3485 1870 Size: 1615 4545 XmnI GAAnn'nnTTC Cuts at: 2873 4289 2873 Size: 1416 4744 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3968 276 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI