Restriction map for
pFN21AB5178
(Linear) (Six-base) MAPSORT of: KIBB5178.seq Check: 2867 from: 1 to: 1455
KIBB5178.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 85 1455
Size: 85 1370
AccI GT'mk_AC
Cuts at: 0 1324 1455
Size: 1324 131
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1455
Size: 676 779
AleI CACnn'nnGTG
Cuts at: 0 622 919 965 1455
Size: 622 297 46 490
Fragments arranged by size:
622 490 297 46
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 499 531 1455
Size: 499 32 924
Fragments arranged by size:
924 499 32
AlwNI CAG_nnn'CTG
Cuts at: 0 567 609 678 925 1182 1455
Size: 567 42 69 247 257 273
Fragments arranged by size:
567 273 257 247 69 42
BanI G'GyrC_C
Cuts at: 0 421 949 1455
Size: 421 528 506
Fragments arranged by size:
528 506 421
BanII G_rGCy'C
Cuts at: 0 890 1316 1455
Size: 890 426 139
Fragments arranged by size:
890 426 139
BbsI GAAGACnn'nnnn_
Cuts at: 0 299 790 1455
Size: 299 491 665
Fragments arranged by size:
665 491 299
BclI T'GATC_A
Cuts at: 0 1395 1455
Size: 1395 60
BglI GCCn_nnn'nGGC
Cuts at: 0 553 1455
Size: 553 902
BlpI GC'TnA_GC
Cuts at: 0 1196 1444 1455
Size: 1196 248 11
Fragments arranged by size:
1196 248 11
Bme1580I G_kGCm'C
Cuts at: 0 952 1004 1455
Size: 952 52 451
Fragments arranged by size:
952 451 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 12 1455
Size: 12 1443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 984 1455
Size: 984 471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 980 1455
Size: 980 475
BsaI GGTCTCn'nnnn_
Cuts at: 0 1078 1455
Size: 1078 377
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 499 529 797 827 1455
Size: 499 30 268 30 628
Fragments arranged by size:
628 499 268 30 30
BseYI C'CCAG_C
Cuts at: 0 602 1455
Size: 602 853
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 634 691 1198 1399 1455
Size: 495 139 57 507 201 56
Fragments arranged by size:
507 495 201 139 57 56
BsiHKAI G_wGCw'C
Cuts at: 0 15 1450 1455
Size: 15 1435 5
Fragments arranged by size:
1435 15 5
Bsp1286I G_dGCh'C
Cuts at: 0 15 890 952 1004 1316 1450 1455
Size: 15 875 62 52 312 134 5
Fragments arranged by size:
875 312 134 62 52 15 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 85 213 1181 1190 1238 1455
Size: 85 128 968 9 48 217
Fragments arranged by size:
968 217 128 85 48 9
BsrDI GCAATG_nn'
Cuts at: 0 317 1251 1455
Size: 317 934 204
Fragments arranged by size:
934 317 204
BsrFI r'CCGG_y
Cuts at: 0 37 1455
Size: 37 1418
BsrGI T'GTAC_A
Cuts at: 0 107 558 1455
Size: 107 451 897
Fragments arranged by size:
897 451 107
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1182 1455
Size: 1182 273
BstEII G'GTnAC_C
Cuts at: 0 97 1359 1455
Size: 97 1262 96
Fragments arranged by size:
1262 97 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 336 624 1455
Size: 336 288 831
Fragments arranged by size:
831 336 288
BstYI r'GATC_y
Cuts at: 0 1162 1455
Size: 1162 293
Bsu36I CC'TnA_GG
Cuts at: 0 49 1346 1455
Size: 49 1297 109
Fragments arranged by size:
1297 109 49
BtgI C'CryG_G
Cuts at: 0 384 617 1455
Size: 384 233 838
Fragments arranged by size:
838 384 233
BtsI GCAGTG_nn'
Cuts at: 0 1406 1455
Size: 1406 49
EaeI y'GGCC_r
Cuts at: 0 326 881 1433 1455
Size: 326 555 552 22
Fragments arranged by size:
555 552 326 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 984 1455
Size: 676 308 471
Fragments arranged by size:
676 471 308
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1060 1455
Size: 1060 395
EcoO109I rG'GnC_Cy
Cuts at: 0 246 537 564 675 1179 1455
Size: 246 291 27 111 504 276
Fragments arranged by size:
504 291 276 246 111 27
FspI TGC'GCA
Cuts at: 0 530 1455
Size: 530 925
FspAI rTGC'GCAy
Cuts at: 0 530 1455
Size: 530 925
HaeII r_GCGC'y
Cuts at: 0 1019 1406 1455
Size: 1019 387 49
Fragments arranged by size:
1019 387 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 970 1002 1023 1055 1455
Size: 970 32 21 32 400
Fragments arranged by size:
970 400 32 32 21
MscI TGG'CCA
Cuts at: 0 328 1435 1455
Size: 328 1107 20
Fragments arranged by size:
1107 328 20
MslI CAynn'nnrTG
Cuts at: 0 622 919 965 1258 1455
Size: 622 297 46 293 197
Fragments arranged by size:
622 297 293 197 46
MspA1I CmG'CkG
Cuts at: 0 159 261 463 606 1443 1455
Size: 159 102 202 143 837 12
Fragments arranged by size:
837 202 159 143 102 12
NcoI C'CATG_G
Cuts at: 0 384 1455
Size: 384 1071
NspI r_CATG'y
Cuts at: 0 360 1455
Size: 360 1095
PasI CC'CwG_GG
Cuts at: 0 579 1455
Size: 579 876
PfoI T'CCnGG_A
Cuts at: 0 974 1455
Size: 974 481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 499 531 1455
Size: 499 32 924
Fragments arranged by size:
924 499 32
PpuMI rG'GwC_Cy
Cuts at: 0 537 1179 1455
Size: 537 642 276
Fragments arranged by size:
642 537 276
PstI C_TGCA'G
Cuts at: 0 96 554 1178 1455
Size: 96 458 624 277
Fragments arranged by size:
624 458 277 96
PvuII CAG'CTG
Cuts at: 0 159 261 463 606 1455
Size: 159 102 202 143 849
Fragments arranged by size:
849 202 159 143 102
SbfI CC_TGCA'GG
Cuts at: 0 554 1178 1455
Size: 554 624 277
Fragments arranged by size:
624 554 277
SfcI C'TryA_G
Cuts at: 0 92 250 472 550 1174 1455
Size: 92 158 222 78 624 281
Fragments arranged by size:
624 281 222 158 92 78
SmlI C'TyrA_G
Cuts at: 0 995 1455
Size: 995 460
SphI G_CATG'C
Cuts at: 0 360 1455
Size: 360 1095
StuI AGG'CCT
Cuts at: 0 647 1455
Size: 647 808
StyI C'CwwG_G
Cuts at: 0 384 449 493 852 1455
Size: 384 65 44 359 603
Fragments arranged by size:
603 384 359 65 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 475 891 1455
Size: 475 416 564
Fragments arranged by size:
564 475 416
TatI w'GTAC_w
Cuts at: 0 107 558 1455
Size: 107 451 897
Fragments arranged by size:
897 451 107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 797 829 1455
Size: 797 32 626
Fragments arranged by size:
797 626 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1146 1455
Size: 1146 309
XmnI GAAnn'nnTTC
Cuts at: 0 868 1455
Size: 868 587
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI BanI BanII
BbsI BclI BglI BlpI Bme1580I BmrI BpmI BpuEI
BsaI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI EaeI Eco57MI EcoNI EcoO109I FspI FspAI HaeII
Hin4I MscI MslI MspA1I NcoI NspI PasI PfoI
PpiI PpuMI PstI PvuII SbfI SfcI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI
BglII BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI ZraI