(Circular) (Six-base) MAPSORT of: pFN21AB5177.seq Check: 3901 from: 1 to: 5356 pFN21AB5177 5356 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3166 278 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2168 2699 1937 Size: 231 531 4594 Fragments arranged by size: 4594 531 231 Acc65I G'GTAC_C Cuts at: 2677 2677 Size: 5356 AclI AA'CG_TT Cuts at: 1563 2500 3483 3856 1563 Size: 937 983 373 3063 Fragments arranged by size: 3063 983 937 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2161 2207 2235 2254 3419 4803 5071 1632 Size: 529 46 28 19 1165 1384 268 1917 Fragments arranged by size: 1917 1384 1165 529 268 46 28 19 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5159 AflIII A'CryG_T Cuts at: 1203 4255 5232 1203 Size: 3052 977 1327 Fragments arranged by size: 3052 1327 977 AgeI A'CCGG_T Cuts at: 4187 4187 Size: 5356 AhdI GACnn_n'nnGTC Cuts at: 4085 4085 Size: 5356 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2069 1184 Size: 885 4471 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2248 2280 2248 Size: 32 5324 AlwNI CAG_nnn'CTG Cuts at: 3099 4671 3099 Size: 1572 3784 ApaLI G'TGCA_C Cuts at: 3413 4569 3413 Size: 1156 4200 ApoI r'AATT_y Cuts at: 1493 2038 2520 2614 2665 2891 3043 5152 1493 Size: 545 482 94 51 226 152 2109 1697 Fragments arranged by size: 2109 1697 545 482 226 152 94 51 AseI AT'TA_AT Cuts at: 160 1032 2250 3910 160 Size: 872 1218 1660 1606 Fragments arranged by size: 1660 1606 1218 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 AvaI C'yCGr_G Cuts at: 1870 1943 2014 2681 1870 Size: 73 71 667 4545 Fragments arranged by size: 4545 667 73 71 BamHI G'GATC_C Cuts at: 2686 3053 2686 Size: 367 4989 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 2677 4132 618 Size: 325 217 627 266 624 1455 1842 Fragments arranged by size: 1842 1455 627 624 325 266 217 BanII G_rGCy'C Cuts at: 1391 2675 1391 Size: 1284 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4385 BbvCI CC'TCA_GC Cuts at: 2149 2149 Size: 5356 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3547 3581 1903 Size: 34 1610 34 3678 Fragments arranged by size: 3678 1610 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2012 2108 3248 4458 2012 Size: 96 1140 1210 2910 Fragments arranged by size: 2910 1210 1140 96 BclI T'GATC_A Cuts at: 1548 2398 1548 Size: 850 4506 BfrBI ATG'CAT Cuts at: 2342 2342 Size: 5356 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3967 136 Size: 107 122 71 631 605 2295 1525 Fragments arranged by size: 2295 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5351 5351 Size: 5356 BlpI GC'TnA_GC Cuts at: 2773 2773 Size: 5356 Bme1580I G_kGCm'C Cuts at: 1790 2645 3417 4573 1790 Size: 855 772 1156 2573 Fragments arranged by size: 2573 1156 855 772 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5356 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3087 4045 442 Size: 416 53 83 399 24 1670 958 1753 Fragments arranged by size: 1753 1670 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4016 1239 Size: 2777 2579 Bpu10I CC'TnA_GC Cuts at: 2149 2149 Size: 5356 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3481 4346 4644 3481 Size: 865 298 4193 Fragments arranged by size: 4193 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2583 4019 882 Size: 617 1084 1436 2219 Fragments arranged by size: 2219 1436 1084 617 BsaAI yAC'GTr Cuts at: 493 1204 3147 493 Size: 711 1943 2702 Fragments arranged by size: 2702 1943 711 BsaBI GATnn'nnATC Cuts at: 2313 5350 2313 Size: 3037 2319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3163 3545 4180 275 Size: 53 83 186 936 1630 382 635 1451 Fragments arranged by size: 1630 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3789 4187 4461 4608 5019 3789 Size: 398 274 147 411 4126 Fragments arranged by size: 4126 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2248 2278 1178 Size: 30 165 30 845 30 4256 Fragments arranged by size: 4256 845 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2040 2096 1186 Size: 854 56 4446 Fragments arranged by size: 4446 854 56 BseYI C'CCAG_C Cuts at: 2095 4559 5055 2095 Size: 2464 496 2396 Fragments arranged by size: 2464 2396 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4783 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2806 3567 4595 1540 Size: 275 186 805 761 1028 2301 Fragments arranged by size: 2301 1028 805 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3417 3502 4573 3417 Size: 85 1071 4200 Fragments arranged by size: 4200 1071 85 BsmI GAATG_Cn' Cuts at: 2046 2067 2342 2867 2960 2046 Size: 21 275 525 93 4442 Fragments arranged by size: 4442 525 275 93 21 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2645 2675 3417 3502 4573 1391 Size: 399 855 30 742 85 1071 2174 Fragments arranged by size: 2174 1071 855 742 399 85 30 BspEI T'CCGG_A Cuts at: 5019 5019 Size: 5356 BspHI T'CATG_A Cuts at: 2388 2428 3245 4975 2388 Size: 40 817 1730 2769 Fragments arranged by size: 2769 1730 817 40 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2291 2711 844 Size: 1447 420 3489 Fragments arranged by size: 3489 1447 420 BsrBI CCG'CTC Cuts at: 1394 1823 3243 1394 Size: 429 1420 3507 Fragments arranged by size: 3507 1420 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3851 4025 65 Size: 1183 2603 174 1396 Fragments arranged by size: 2603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4000 4187 1700 Size: 110 2190 187 2869 Fragments arranged by size: 2869 2190 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5356 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5356 BssSI C'ACGA_G Cuts at: 3416 4428 3416 Size: 1012 4344 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5356 BstBI TT'CG_AA Cuts at: 4160 4160 Size: 5356 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5356 BstYI r'GATC_y Cuts at: 1972 2686 3053 3438 3455 4907 4993 5005 5351 Size: 714 367 385 17 1452 86 12 346 Cuts at: 5351 1972 Size: 1977 Fragments arranged by size: 1977 1452 714 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2169 2169 Size: 5356 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3865 Fragments arranged by size: 3865 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4617 Fragments arranged by size: 4617 683 56 BtsI GCAGTG_nn' Cuts at: 2872 3666 3686 2872 Size: 794 20 4542 Fragments arranged by size: 4542 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5356 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5321 DraI TTT'AAA Cuts at: 2660 2660 Size: 5356 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4363 809 Size: 507 3047 1802 Fragments arranged by size: 3047 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2803 3692 5321 Size: 54 1425 50 275 18 973 889 1629 Cuts at: 5321 8 Size: 43 Fragments arranged by size: 1629 1425 973 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2803 1537 Size: 275 991 4090 Fragments arranged by size: 4090 991 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3286 1353 Size: 1933 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3943 4317 4463 387 Size: 1064 138 2354 374 146 1280 Fragments arranged by size: 2354 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2161 2207 2235 2254 3419 4016 4803 Size: 393 529 46 28 19 1165 597 787 Cuts at: 4803 5071 1239 Size: 268 1524 Fragments arranged by size: 1524 1165 787 597 529 393 268 46 28 19 EcoRI G'AATT_C Cuts at: 2665 3043 2665 Size: 378 4978 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 5356 FspI TGC'GCA Cuts at: 3862 3862 Size: 5356 HaeII r_GCGC'y Cuts at: 1678 4503 1678 Size: 2825 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2383 2415 3997 Size: 32 135 32 530 32 249 32 1582 Cuts at: 3997 4029 4071 4103 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1582 530 249 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2700 2946 669 Size: 988 281 762 246 3079 Fragments arranged by size: 3079 988 762 281 246 HindIII A'AGCT_T Cuts at: 748 2555 748 Size: 1807 3549 HpaI GTT'AAC Cuts at: 2946 2946 Size: 5356 KpnI G_GTAC'C Cuts at: 2681 2681 Size: 5356 MfeI C'AATT_G Cuts at: 2652 2955 2652 Size: 303 5053 MluI A'CGCG_T Cuts at: 5232 5232 Size: 5356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2286 4470 4654 1305 Size: 981 2184 184 2007 Fragments arranged by size: 2184 2007 981 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3534 Fragments arranged by size: 3534 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2051 3314 3673 3832 518 Size: 648 885 1263 359 159 2042 Fragments arranged by size: 2042 1263 885 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2153 2772 3449 4597 4842 1606 Size: 547 619 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 619 547 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3865 Fragments arranged by size: 3865 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5356 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5356 NotI GC'GGCC_GC Cuts at: 2803 2803 Size: 5356 NsiI A_TGCA'T Cuts at: 2344 2344 Size: 5356 NspI r_CATG'y Cuts at: 1132 1229 2714 4259 1132 Size: 97 1485 1545 2229 Fragments arranged by size: 2229 1545 1485 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5356 PciI A'CATG_T Cuts at: 4255 4255 Size: 5356 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5356 PmeI GTTT'AAAC Cuts at: 2660 2660 Size: 5356 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2248 2280 3425 3457 4938 4970 2248 Size: 32 1145 32 1481 32 2634 Fragments arranged by size: 2634 1481 1145 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5356 PsiI TTA'TAA Cuts at: 2926 2926 Size: 5356 PstI C_TGCA'G Cuts at: 830 2288 2708 830 Size: 1458 420 3478 Fragments arranged by size: 3478 1458 420 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5356 PvuII CAG'CTG Cuts at: 2153 2153 Size: 5356 SalI G'TCGA_C Cuts at: 1936 2698 1936 Size: 762 4594 SbfI CC_TGCA'GG Cuts at: 2708 2708 Size: 5356 ScaI AGT'ACT Cuts at: 3604 5316 3604 Size: 1712 3644 SfcI C'TryA_G Cuts at: 826 1045 2284 2704 3839 4520 4711 826 Size: 219 1239 420 1135 681 191 1471 Fragments arranged by size: 1471 1239 1135 681 420 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5356 SmaI CCC'GGG Cuts at: 1872 2016 2683 1872 Size: 144 667 4545 Fragments arranged by size: 4545 667 144 SmlI C'TyrA_G Cuts at: 820 1017 1943 3460 4361 4623 820 Size: 197 926 1517 901 262 1553 Fragments arranged by size: 1553 1517 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3147 493 Size: 2654 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5356 SphI G_CATG'C Cuts at: 1132 2714 1132 Size: 1582 3774 SspI AAT'ATT Cuts at: 5 52 3280 5 Size: 47 3228 2081 Fragments arranged by size: 3228 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5356 StyI C'CwwG_G Cuts at: 513 1065 2004 2795 513 Size: 552 939 791 3074 Fragments arranged by size: 3074 939 791 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3383 3400 3553 3738 4183 325 Size: 1255 1803 17 153 185 445 1498 Fragments arranged by size: 1803 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3602 5314 96 Size: 274 80 33 51 1443 1625 1712 138 Fragments arranged by size: 1712 1625 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2064 2096 5316 5348 2064 Size: 32 3220 32 2072 Fragments arranged by size: 3220 2072 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5356 XbaI T'CTAG_A Cuts at: 2692 2692 Size: 5356 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2452 2452 Size: 5356 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5356 XmaI C'CCGG_G Cuts at: 1870 2014 2681 1870 Size: 144 667 4545 Fragments arranged by size: 4545 667 144 XmnI GAAnn'nnTTC Cuts at: 2220 3485 2220 Size: 1265 4091 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3164 276 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI