Restriction map for
pFN21AB5161
##################################
# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MflI MluI MroI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 1372 AAATTT [GA]^AATT[CT]
AflIII 1359 ACATGT A^C[GA][CT]GT
AlwNI 88 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1372 AAATTT [GA]^AATT[CT]
AseI 543 ATTAAT AT^TAAT
AsnI 543 ATTAAT AT^TAAT
BglI 70 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 350 GAGGAG GAGGAG(10/8)
BsiYI 41 CCACCCTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 41 CCACCCTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspLU11I 1359 ACATGT A^CATGT
BsrFI 56 ACCGGC [GA]^CCGG[CT]
BssSI 1232 CACGAG CACGAG(-5/-1)
BstAPI 446 GCAGAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 1146 GCGC GCG^C
Cfr10I 56 ACCGGC [GA]^CCGG[CT]
DraII 534 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 557 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 534 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 120 CCATGG C^C[AT][AT]GG
FauI 75 GCGGG CCCGC(4/6)
HapII 57 CCGG C^CGG
HhaI 1146 GCGC GCG^C
HinP1I 1146 GCGC G^CGC
HpaII 57 CCGG C^CGG
HphI 235 TCACC GGTGA(8/7)
MfeI 254 CAATTG C^AATTG
MspA1I 73 CAGCGG C[AC]G^C[GT]G
MspI 57 CCGG C^CGG
MunI 254 CAATTG C^AATTG
NcoI 120 CCATGG C^CATGG
NspI 1359 ACATGT [GA]CATG^[CT]
PpuMI 534 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 543 ATTAAT AT^TAAT
SspI 691 AATATT AAT^ATT
StyI 120 CCATGG C^C[AT][AT]GG
XcmI 215 CCAAAGGATATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaII 535 GGTCC G^G[AT]CC
881 GGACC
BalI 38 TGGCCA TGG^CCA
986 TGGCCA
BsmAI 592 GAGAC GTCTC(1/5)
205 GTCTC
BsmFI 899 GGGAC GGGAC(10/14)
1018 GGGAC
BsrI 878 ACTGG ACTGG(1/-1)
1110 ACTGG
BssKI 488 CCAGG ^CC[ACGT]GG
885 CCAGG
BstNI 488 CCAGG CC^[AT]GG
885 CCAGG
DpnII 822 GATC ^GATC
868 GATC
DsaI 35 CCGTGG C^C[GA][CT]GG
120 CCATGG
EcoRII 488 CCAGG ^CC[AT]GG
885 CCAGG
HincII 568 GTCAAC GT[CT]^[GA]AC
1123 GTCAAC
HindII 568 GTCAAC GT[CT]^[GA]AC
1123 GTCAAC
MboI 822 GATC ^GATC
868 GATC
MluNI 38 TGGCCA TGG^CCA
986 TGGCCA
MscI 38 TGGCCA TGG^CCA
986 TGGCCA
MvaI 488 CCAGG CC^[AT]GG
885 CCAGG
NdeII 822 GATC ^GATC
868 GATC
NlaIV 50 GGAACC GG[ACGT]^[ACGT]CC
881 GGACCC
Sau3AI 822 GATC ^GATC
868 GATC
ScrFI 488 CCAGG CC^[ACGT]GG
885 CCAGG
SfcI 871 CTACAG C^T[GA][CT]AG
1055 CTACAG
TaqI 941 TCGA T^CGA
1238 TCGA
Tsp45I 234 GTCAC ^GT[GC]AC
1339 GTGAC
TspRI 1253 CACTG CA[GC]TG[ACGT][ACGT]^
1266 CAGTG
TthHB8I 941 TCGA T^CGA
1238 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BpmI BsaJI BstF5I Cac8I Cfr13I
DdeI EaeI Fnu4HI FokI HaeIII
HindIII HinfI ItaI MaeI MaeIII
MboII MnlI MseI MslI MwoI
NlaIII PleI RsaI Sau96I SfaNI
TfiI Tru9I TseI Tsp509I