Restriction map for
pFN21AB5161
(Circular) (Six-base) MAPSORT of: pFN21AB5161.seq Check: 1454 from: 1 to: 6094
pFN21AB5161 6094 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3904 278
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3437 1937
Size: 1500 4594
Acc65I G'GTAC_C
Cuts at: 3415 3415
Size: 6094
AclI AA'CG_TT
Cuts at: 1563 4221 4594 1563
Size: 2658 373 3063
Fragments arranged by size:
3063 2658 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2625 3084 4157 5541 5809 1632
Size: 993 459 1073 1384 268 1917
Fragments arranged by size:
1917 1384 1073 993 459 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5897
AflIII A'CryG_T
Cuts at: 1203 3364 4993 5970 1203
Size: 2161 1629 977 1327
Fragments arranged by size:
2161 1629 1327 977
AgeI A'CCGG_T
Cuts at: 4925 4925
Size: 6094
AhdI GACnn_n'nnGTC
Cuts at: 4823 4823
Size: 6094
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6094
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6094
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2646 2678 2646
Size: 32 6062
AlwNI CAG_nnn'CTG
Cuts at: 2098 3837 5409 2098
Size: 1739 1572 2783
Fragments arranged by size:
2783 1739 1572
ApaLI G'TGCA_C
Cuts at: 4151 5307 4151
Size: 1156 4938
ApoI r'AATT_y
Cuts at: 1493 3377 3403 3629 3781 5890 1493
Size: 1884 26 226 152 2109 1697
Fragments arranged by size:
2109 1884 1697 226 152 26
AseI AT'TA_AT
Cuts at: 160 1032 2549 4648 160
Size: 872 1517 2099 1606
Fragments arranged by size:
2099 1606 1517 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
AvaI C'yCGr_G
Cuts at: 1870 1943 3419 1870
Size: 73 1476 4545
Fragments arranged by size:
4545 1476 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3225 3258 3225
Size: 33 6061
BamHI G'GATC_C
Cuts at: 3424 3791 3424
Size: 367 5727
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3415 4870 618
Size: 325 217 627 1628 1455 1842
Fragments arranged by size:
1842 1628 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3413 1391
Size: 2022 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4285 4319 1903
Size: 34 2348 34 3678
Fragments arranged by size:
3678 2348 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3986 5196 3986
Size: 1210 4884
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6094
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2081 4705
Size: 107 122 71 631 605 337 72 2624
Cuts at: 4705 136
Size: 1525
Fragments arranged by size:
2624 1525 631 605 337 122 107 72
71
BglII A'GATC_T
Cuts at: 6089 6089
Size: 6094
BlpI GC'TnA_GC
Cuts at: 3511 3511
Size: 6094
Bme1580I G_kGCm'C
Cuts at: 1790 4155 5311 1790
Size: 2365 1156 2573
Fragments arranged by size:
2573 2365 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6094
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3825 4783 442
Size: 416 53 83 399 24 2408 958 1753
Fragments arranged by size:
2408 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2361 2648 3012 4754 1239
Size: 1122 287 364 1742 2579
Fragments arranged by size:
2579 1742 1122 364 287
Bpu10I CC'TnA_GC
Cuts at: 2091 2919 3252 3349 2091
Size: 828 333 97 4836
Fragments arranged by size:
4836 828 333 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4219 5084 5382 4219
Size: 865 298 4931
Fragments arranged by size:
4931 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4757 882
Size: 617 3258 2219
Fragments arranged by size:
3258 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3885 493
Size: 711 2681 2702
Fragments arranged by size:
2702 2681 711
BsaBI GATnn'nnATC
Cuts at: 6088 6088
Size: 6094
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3901 4283 4918 275
Size: 53 83 186 936 2368 382 635 1451
Fragments arranged by size:
2368 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4527 4925 5199 5346 5757 4527
Size: 398 274 147 411 4864
Fragments arranged by size:
4864 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2646 2676 1178
Size: 30 165 30 1243 30 4596
Fragments arranged by size:
4596 1243 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2370 1186
Size: 1184 4910
BseYI C'CCAG_C
Cuts at: 2076 5297 5793 2076
Size: 3221 496 2377
Fragments arranged by size:
3221 2377 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5521
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3544 4305 5333 1540
Size: 275 186 1543 761 1028 2301
Fragments arranged by size:
2301 1543 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4155 4240 5311 4155
Size: 85 1071 4938
Fragments arranged by size:
4938 1071 85
BsmI GAATG_Cn'
Cuts at: 3605 3698 3605
Size: 93 6001
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3413 4155 4240 5311 1391
Size: 399 1623 742 85 1071 2174
Fragments arranged by size:
2174 1623 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5757 5757
Size: 6094
BspHI T'CATG_A
Cuts at: 3983 5713 3983
Size: 1730 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3449 844
Size: 2605 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3981 1394
Size: 429 2158 3507
Fragments arranged by size:
3507 2158 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4589 4763 65
Size: 1183 3341 174 1396
Fragments arranged by size:
3341 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2061 4738 4925 1700
Size: 110 251 2677 187 2869
Fragments arranged by size:
2869 2677 251 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6094
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6094
BssSI C'ACGA_G
Cuts at: 3237 4154 5166 3237
Size: 917 1012 4165
Fragments arranged by size:
4165 1012 917
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2457 794
Size: 1663 4431
BstBI TT'CG_AA
Cuts at: 4898 4898
Size: 6094
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6094
BstYI r'GATC_y
Cuts at: 1972 3424 3791 4176 4193 5645 5731 5743 6089
Size: 1452 367 385 17 1452 86 12 346
Cuts at: 6089 1972
Size: 1977
Fragments arranged by size:
1977 1452 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 2125 513
Size: 552 939 36 85 4482
Fragments arranged by size:
4482 939 552 85 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5355
Fragments arranged by size:
5355 683 56
BtsI GCAGTG_nn'
Cuts at: 3610 4404 4424 3610
Size: 794 20 5280
Fragments arranged by size:
5280 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6059
DraI TTT'AAA
Cuts at: 3398 3398
Size: 6094
DraIII CAC_nnn'GTG
Cuts at: 2567 2567
Size: 6094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5101 809
Size: 507 3785 1802
Fragments arranged by size:
3785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2043 2991
Size: 54 1425 50 275 18 207 6 948
Cuts at: 2991 3541 4430 6059 8
Size: 550 889 1629 43
Fragments arranged by size:
1629 1425 948 889 550 275 207 54
50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3541 1537
Size: 275 1729 4090
Fragments arranged by size:
4090 1729 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4024 1353
Size: 2671 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4681 5055 5201 387
Size: 1064 138 3092 374 146 1280
Fragments arranged by size:
3092 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2361 2625 2648 3012 3084 4157 4754
Size: 393 729 264 23 364 72 1073 597
Cuts at: 4754 5541 5809 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1073 787 729 597 393 364 268
264 72 23
EcoO109I rG'GnC_Cy
Cuts at: 2540 2540
Size: 6094
EcoRI G'AATT_C
Cuts at: 3403 3781 3403
Size: 378 5716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 6094
FspI TGC'GCA
Cuts at: 4600 4600
Size: 6094
HaeII r_GCGC'y
Cuts at: 1678 5241 1678
Size: 3563 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4735 4767 4809 4841 1373
Size: 32 135 32 3163 32 42 32 2626
Fragments arranged by size:
3163 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2575 3130 3438 3684 669
Size: 988 281 637 555 308 246 3079
Fragments arranged by size:
3079 988 637 555 308 281 246
HindIII A'AGCT_T
Cuts at: 748 2250 2729 3089 748
Size: 1502 479 360 3753
Fragments arranged by size:
3753 1502 479 360
HpaI GTT'AAC
Cuts at: 3684 3684
Size: 6094
KpnI G_GTAC'C
Cuts at: 3419 3419
Size: 6094
MfeI C'AATT_G
Cuts at: 2259 3693 2259
Size: 1434 4660
MluI A'CGCG_T
Cuts at: 5970 5970
Size: 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5208 5392 1305
Size: 3903 184 2007
Fragments arranged by size:
3903 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 2993 10
Size: 54 1425 343 213 948 3111
Fragments arranged by size:
3111 1425 948 343 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 2568 3369 4052 4411 4570 518
Size: 648 1017 385 801 683 359 159 2042
Fragments arranged by size:
2042 1017 801 683 648 385 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 3510 4187 5335 5580 1606
Size: 474 1430 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 677 474 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2125 513
Size: 552 939 121 4482
Fragments arranged by size:
4482 939 552 121
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6094
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6094
NotI GC'GGCC_GC
Cuts at: 3541 3541
Size: 6094
NspI r_CATG'y
Cuts at: 1132 1229 3368 3452 4997 1132
Size: 97 2139 84 1545 2229
Fragments arranged by size:
2229 2139 1545 97 84
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6094
PciI A'CATG_T
Cuts at: 3364 4993 3364
Size: 1629 4465
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6094
PmeI GTTT'AAAC
Cuts at: 3398 3398
Size: 6094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2646 2678 4163 4195 5676 5708 2646
Size: 32 1485 32 1481 32 3032
Fragments arranged by size:
3032 1485 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2540 2540
Size: 6094
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6094
PsiI TTA'TAA
Cuts at: 3664 3664
Size: 6094
PstI C_TGCA'G
Cuts at: 830 3446 830
Size: 2616 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6094
SalI G'TCGA_C
Cuts at: 1936 3436 1936
Size: 1500 4594
SbfI CC_TGCA'GG
Cuts at: 3446 3446
Size: 6094
ScaI AGT'ACT
Cuts at: 4342 6054 4342
Size: 1712 4382
SfcI C'TryA_G
Cuts at: 826 1045 2876 3060 3442 4577 5258 5449 826
Size: 219 1831 184 382 1135 681 191 1471
Fragments arranged by size:
1831 1471 1135 681 382 219 191 184
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6094
SmaI CCC'GGG
Cuts at: 1872 3421 1872
Size: 1549 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4198 5099 5361 820
Size: 197 926 2255 901 262 1553
Fragments arranged by size:
2255 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3885 493
Size: 3392 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6094
SphI G_CATG'C
Cuts at: 1132 3452 1132
Size: 2320 3774
SspI AAT'ATT
Cuts at: 5 52 2698 4018 5
Size: 47 2646 1320 2081
Fragments arranged by size:
2646 2081 1320 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6094
StyI C'CwwG_G
Cuts at: 513 1065 2004 2125 3533 513
Size: 552 939 121 1408 3074
Fragments arranged by size:
3074 1408 939 552 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4121 4138 4291 4476 4921 325
Size: 1255 2541 17 153 185 445 1498
Fragments arranged by size:
2541 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2137 4340 6052
Size: 274 80 33 51 1443 160 2203 1712
Cuts at: 6052 96
Size: 138
Fragments arranged by size:
2203 1712 1443 274 160 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6054 6086 6054
Size: 32 6062
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6094
XbaI T'CTAG_A
Cuts at: 3430 3430
Size: 6094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2227 2227
Size: 6094
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6094
XmaI C'CCGG_G
Cuts at: 1870 3419 1870
Size: 1549 4545
XmnI GAAnn'nnTTC
Cuts at: 4223 4223
Size: 6094
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3902 276
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI