Restriction map for
pFN21AB5161
(Linear) (Six-base) MAPSORT of: KIBB5161.seq Check: 1318 from: 1 to: 1389
KIBB5161.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 1079 1389
Size: 620 459 310
Fragments arranged by size:
620 459 310
AflIII A'CryG_T
Cuts at: 0 1359 1389
Size: 1359 30
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 641 673 1389
Size: 641 32 716
Fragments arranged by size:
716 641 32
AlwNI CAG_nnn'CTG
Cuts at: 0 93 1389
Size: 93 1296
ApoI r'AATT_y
Cuts at: 0 1372 1389
Size: 1372 17
AseI AT'TA_AT
Cuts at: 0 544 1389
Size: 544 845
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1220 1253 1389
Size: 1220 33 136
Fragments arranged by size:
1220 136 33
BglI GCCn_nnn'nGGC
Cuts at: 0 76 1389
Size: 76 1313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 643 1007 1389
Size: 356 287 364 382
Fragments arranged by size:
382 364 356 287
Bpu10I CC'TnA_GC
Cuts at: 0 86 914 1247 1344 1389
Size: 86 828 333 97 45
Fragments arranged by size:
828 333 97 86 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 641 671 1389
Size: 641 30 718
Fragments arranged by size:
718 641 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 365 1389
Size: 365 1024
BseYI C'CCAG_C
Cuts at: 0 71 1389
Size: 71 1318
BsrFI r'CCGG_y
Cuts at: 0 56 1389
Size: 56 1333
BssSI C'ACGA_G
Cuts at: 0 1232 1389
Size: 1232 157
BstAPI GCAn_nnn'nTGC
Cuts at: 0 452 1389
Size: 452 937
BtgI C'CryG_G
Cuts at: 0 35 120 1389
Size: 35 85 1269
Fragments arranged by size:
1269 85 35
DraIII CAC_nnn'GTG
Cuts at: 0 562 1389
Size: 562 827
EaeI y'GGCC_r
Cuts at: 0 32 38 986 1389
Size: 32 6 948 403
Fragments arranged by size:
948 403 32 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 620 643 1007 1079 1389
Size: 356 264 23 364 72 310
Fragments arranged by size:
364 356 310 264 72 23
EcoO109I rG'GnC_Cy
Cuts at: 0 535 1389
Size: 535 854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 879 1389
Size: 879 510
HincII GTy'rAC
Cuts at: 0 570 1125 1389
Size: 570 555 264
Fragments arranged by size:
570 555 264
HindIII A'AGCT_T
Cuts at: 0 245 724 1084 1389
Size: 245 479 360 305
Fragments arranged by size:
479 360 305 245
MfeI C'AATT_G
Cuts at: 0 254 1389
Size: 254 1135
MscI TGG'CCA
Cuts at: 0 40 988 1389
Size: 40 948 401
Fragments arranged by size:
948 401 40
MslI CAynn'nnrTG
Cuts at: 0 178 563 1364 1389
Size: 178 385 801 25
Fragments arranged by size:
801 385 178 25
MspA1I CmG'CkG
Cuts at: 0 75 1389
Size: 75 1314
NcoI C'CATG_G
Cuts at: 0 120 1389
Size: 120 1269
NspI r_CATG'y
Cuts at: 0 1363 1389
Size: 1363 26
PciI A'CATG_T
Cuts at: 0 1359 1389
Size: 1359 30
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 641 673 1389
Size: 641 32 716
Fragments arranged by size:
716 641 32
PpuMI rG'GwC_Cy
Cuts at: 0 535 1389
Size: 535 854
SfcI C'TryA_G
Cuts at: 0 871 1055 1389
Size: 871 184 334
Fragments arranged by size:
871 334 184
SspI AAT'ATT
Cuts at: 0 693 1389
Size: 693 696
StyI C'CwwG_G
Cuts at: 0 120 1389
Size: 120 1269
TatI w'GTAC_w
Cuts at: 0 132 1389
Size: 132 1257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 222 1389
Size: 222 1167
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApoI AseI BaeI BglI
BpmI Bpu10I BsaXI BseRI BseYI BsrFI BssSI BstAPI
BtgI DraIII EaeI Eco57MI EcoO109I FalI HincII HindIII
MfeI MscI MslI MspA1I NcoI NspI PciI PpiI
PpuMI SfcI SspI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII Hin4I HpaI KasI
KpnI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI