(Circular) (Six-base) MAPSORT of: pFN21AB5141.seq Check: 4799 from: 1 to: 6274 pFN21AB5141 6274 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2161 2326 2161 Size: 165 6109 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4084 278 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3617 1937 Size: 1680 4594 Acc65I G'GTAC_C Cuts at: 3595 3595 Size: 6274 AclI AA'CG_TT Cuts at: 1563 4401 4774 1563 Size: 2838 373 3063 Fragments arranged by size: 3063 2838 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2609 3466 4337 5721 5989 1632 Size: 977 857 871 1384 268 1917 Fragments arranged by size: 1917 1384 977 871 857 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6077 AflIII A'CryG_T Cuts at: 1203 2919 3393 5173 6150 1203 Size: 1716 474 1780 977 1327 Fragments arranged by size: 1780 1716 1327 977 474 AgeI A'CCGG_T Cuts at: 5105 5105 Size: 6274 AhdI GACnn_n'nnGTC Cuts at: 5003 5003 Size: 6274 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6274 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3323 1184 Size: 2139 4135 AlwNI CAG_nnn'CTG Cuts at: 2061 3143 4017 5589 2061 Size: 1082 874 1572 2746 Fragments arranged by size: 2746 1572 1082 874 ApaI G_GGCC'C Cuts at: 2979 2979 Size: 6274 ApaLI G'TGCA_C Cuts at: 4331 5487 4331 Size: 1156 5118 ApoI r'AATT_y Cuts at: 1493 3291 3583 3809 3961 6070 1493 Size: 1798 292 226 152 2109 1697 Fragments arranged by size: 2109 1798 1697 292 226 152 AseI AT'TA_AT Cuts at: 160 1032 4828 160 Size: 872 3796 1606 Fragments arranged by size: 3796 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 AvaI C'yCGr_G Cuts at: 1870 1943 2120 2234 2978 3599 1870 Size: 73 177 114 744 621 4545 Fragments arranged by size: 4545 744 621 177 114 73 BamHI G'GATC_C Cuts at: 2415 3604 3971 2415 Size: 1189 367 4718 Fragments arranged by size: 4718 1189 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3595 5050 618 Size: 325 217 627 1808 1455 1842 Fragments arranged by size: 1842 1808 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2235 2682 2748 2979 3003 3593 1391 Size: 844 447 66 231 24 590 4072 Fragments arranged by size: 4072 844 590 447 231 66 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3273 928 Size: 971 1374 3929 Fragments arranged by size: 3929 1374 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3418 3452 4465 4499 1903 Size: 34 50 34 1397 34 1013 34 3678 Fragments arranged by size: 3678 1397 1013 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4166 5376 4166 Size: 1210 5064 BclI T'GATC_A Cuts at: 1548 3072 1548 Size: 1524 4750 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2093 4885 136 Size: 107 122 71 631 605 421 2792 1525 Fragments arranged by size: 2792 1525 631 605 421 122 107 71 BglII A'GATC_T Cuts at: 6269 6269 Size: 6274 BlpI GC'TnA_GC Cuts at: 3691 3691 Size: 6274 Bme1580I G_kGCm'C Cuts at: 1790 2410 2979 3134 3444 4335 5491 1790 Size: 620 569 155 310 891 1156 2573 Fragments arranged by size: 2573 1156 891 620 569 310 155 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6274 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3131 4005 4963 Size: 416 53 83 399 24 1714 874 958 Cuts at: 4963 442 Size: 1753 Fragments arranged by size: 1753 1714 958 874 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2463 1056 Size: 1407 4867 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3022 3022 Size: 6274 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2913 2971 3372 3437 3486 3581 4934 Size: 935 739 58 401 65 49 95 1353 Cuts at: 4934 1239 Size: 2579 Fragments arranged by size: 2579 1353 935 739 401 95 65 58 49 Bpu10I CC'TnA_GC Cuts at: 3369 3369 Size: 6274 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2093 2198 4399 5264 5562 2093 Size: 105 2201 865 298 2805 Fragments arranged by size: 2805 2201 865 298 105 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4937 882 Size: 617 3438 2219 Fragments arranged by size: 3438 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4065 493 Size: 711 2861 2702 Fragments arranged by size: 2861 2702 711 BsaBI GATnn'nnATC Cuts at: 2639 3491 6268 2639 Size: 852 2777 2645 Fragments arranged by size: 2777 2645 852 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2295 2685 4081 4463 Size: 53 83 186 936 762 390 1396 382 Cuts at: 4463 5098 275 Size: 635 1451 Fragments arranged by size: 1451 1396 936 762 635 390 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4707 5105 5379 5526 5937 4707 Size: 398 274 147 411 5044 Fragments arranged by size: 5044 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3005 3035 1178 Size: 30 165 30 1602 30 4417 Fragments arranged by size: 4417 1602 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2729 3069 3206 3434 1186 Size: 1543 340 137 228 4026 Fragments arranged by size: 4026 1543 340 228 137 BseYI C'CCAG_C Cuts at: 2054 2378 2724 5477 5973 2054 Size: 324 346 2753 496 2355 Fragments arranged by size: 2753 2355 496 346 324 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2780 3065 1163 Size: 573 1044 285 4372 Fragments arranged by size: 4372 1044 573 285 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2088 3724 4485 5513 1540 Size: 275 186 87 1636 761 1028 2301 Fragments arranged by size: 2301 1636 1028 761 275 186 87 BsiHKAI G_wGCw'C Cuts at: 2109 2214 2235 2682 2748 2796 3003 4335 4420 Size: 105 21 447 66 48 207 1332 85 Cuts at: 4420 5491 2109 Size: 1071 2892 Fragments arranged by size: 2892 1332 1071 447 207 105 85 66 48 21 BsmI GAATG_Cn' Cuts at: 3785 3878 3785 Size: 93 6181 BsmBI CGTCTCn'nnnn_ Cuts at: 2605 2605 Size: 6274 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2214 2235 2410 2682 2748 2796 Size: 399 319 105 21 175 272 66 48 Cuts at: 2796 2979 3003 3134 3444 3593 4335 4420 5491 Size: 183 24 131 310 149 742 85 1071 Cuts at: 5491 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 319 310 272 183 175 149 131 105 85 66 48 24 21 BspEI T'CCGG_A Cuts at: 5937 5937 Size: 6274 BspHI T'CATG_A Cuts at: 4163 5893 4163 Size: 1730 4544 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2161 2326 2373 3087 3629 844 Size: 1317 165 47 714 542 3489 Fragments arranged by size: 3489 1317 714 542 165 47 BsrBI CCG'CTC Cuts at: 1394 1823 4161 1394 Size: 429 2338 3507 Fragments arranged by size: 3507 2338 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4769 4943 65 Size: 1183 3521 174 1396 Fragments arranged by size: 3521 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2130 2219 4918 5105 1700 Size: 110 320 89 2699 187 2869 Fragments arranged by size: 2869 2699 320 187 110 89 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6274 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6274 BssSI C'ACGA_G Cuts at: 4334 5346 4334 Size: 1012 5262 BstAPI GCAn_nnn'nTGC Cuts at: 794 2844 794 Size: 2050 4224 BstBI TT'CG_AA Cuts at: 5078 5078 Size: 6274 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6274 BstXI CCAn_nnnn'nTGG Cuts at: 2995 3358 2995 Size: 363 5911 BstYI r'GATC_y Cuts at: 1972 2415 2592 3336 3604 3971 4356 4373 5825 Size: 443 177 744 268 367 385 17 1452 Cuts at: 5825 5911 5923 6269 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 744 443 385 367 346 268 177 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2787 2787 Size: 6274 BtgI C'CryG_G Cuts at: 513 1065 2556 3156 513 Size: 552 1491 600 3631 Fragments arranged by size: 3631 1491 600 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5535 Fragments arranged by size: 5535 683 56 BtsI GCAGTG_nn' Cuts at: 3295 3454 3790 4584 4604 3295 Size: 159 336 794 20 4965 Fragments arranged by size: 4965 794 336 159 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6274 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6239 DraI TTT'AAA Cuts at: 3578 3578 Size: 6274 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5281 809 Size: 507 3965 1802 Fragments arranged by size: 3965 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 3243 3721 Size: 54 1425 50 275 18 255 1158 478 Cuts at: 3721 4610 6239 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1158 889 478 275 255 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2085 3721 1537 Size: 275 273 1636 4090 Fragments arranged by size: 4090 1636 275 273 EarI CTCTTCn'nnn_ Cuts at: 1353 2871 4204 1353 Size: 1518 1333 3423 Fragments arranged by size: 3423 1518 1333 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2428 4861 5235 5381 387 Size: 1064 138 839 2433 374 146 1280 Fragments arranged by size: 2433 1280 1064 839 374 146 138 EcoICRI GAG'CTC Cuts at: 2233 2680 2746 3001 2233 Size: 447 66 255 5506 Fragments arranged by size: 5506 447 255 66 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2609 2913 2971 3372 3437 3466 Size: 393 542 435 304 58 401 65 29 Cuts at: 3466 3486 3581 4337 4934 5721 5989 1239 Size: 20 95 756 597 787 268 1524 Fragments arranged by size: 1524 787 756 597 542 435 401 393 304 268 95 65 58 29 20 EcoNI CCTnn'n_nnAGG Cuts at: 2333 2711 2333 Size: 378 5896 EcoO109I rG'GnC_Cy Cuts at: 2201 2448 2975 2201 Size: 247 527 5500 Fragments arranged by size: 5500 527 247 EcoRI G'AATT_C Cuts at: 3291 3583 3961 3291 Size: 292 378 5604 Fragments arranged by size: 5604 378 292 FspI TGC'GCA Cuts at: 4780 4780 Size: 6274 HaeII r_GCGC'y Cuts at: 1678 2024 2732 5421 1678 Size: 346 708 2689 2531 Fragments arranged by size: 2689 2531 708 346 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2271 2303 3209 3241 4915 Size: 32 135 32 699 32 906 32 1674 Cuts at: 4915 4947 4989 5021 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1674 906 699 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2473 3618 3864 669 Size: 988 281 535 1145 246 3079 Fragments arranged by size: 3079 1145 988 535 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6274 HpaI GTT'AAC Cuts at: 3864 3864 Size: 6274 KpnI G_GTAC'C Cuts at: 3599 3599 Size: 6274 MfeI C'AATT_G Cuts at: 3873 3873 Size: 6274 MluI A'CGCG_T Cuts at: 6150 6150 Size: 6274 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5388 5572 1305 Size: 4083 184 2007 Fragments arranged by size: 4083 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3245 10 Size: 54 1425 343 1413 3039 Fragments arranged by size: 3039 1425 1413 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2693 3068 3167 4232 4591 4750 518 Size: 648 1527 375 99 1065 359 159 2042 Fragments arranged by size: 2042 1527 1065 648 375 359 159 99 MspA1I CmG'CkG Cuts at: 1606 2011 2245 2666 3103 3690 4367 5515 5760 Size: 405 234 421 437 587 677 1148 245 Cuts at: 5760 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 587 437 421 405 245 234 NaeI GCC'GGC Cuts at: 2132 2221 2132 Size: 89 6185 NcoI C'CATG_G Cuts at: 513 1065 3156 513 Size: 552 2091 3631 Fragments arranged by size: 3631 2091 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6274 NgoMIV G'CCGG_C Cuts at: 2130 2219 2130 Size: 89 6185 NheI G'CTAG_C Cuts at: 1052 2459 1052 Size: 1407 4867 NotI GC'GGCC_GC Cuts at: 2085 3721 2085 Size: 1636 4638 NspI r_CATG'y Cuts at: 1132 1229 3397 3632 5177 1132 Size: 97 2168 235 1545 2229 Fragments arranged by size: 2229 2168 1545 235 97 PasI CC'CwG_GG Cuts at: 1348 2810 1348 Size: 1462 4812 PciI A'CATG_T Cuts at: 3393 5173 3393 Size: 1780 4494 PflMI CCAn_nnn'nTGG Cuts at: 1108 2061 2305 3143 3382 1108 Size: 953 244 838 239 4000 Fragments arranged by size: 4000 953 838 244 239 PfoI T'CCnGG_A Cuts at: 3414 3558 3414 Size: 144 6130 PmeI GTTT'AAAC Cuts at: 3578 3578 Size: 6274 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4343 4375 5856 5888 4343 Size: 32 1481 32 4729 Fragments arranged by size: 4729 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2201 2448 2201 Size: 247 6027 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6274 PsiI TTA'TAA Cuts at: 3844 3844 Size: 6274 PspOMI G'GGCC_C Cuts at: 2975 2975 Size: 6274 PspXI vC'TCGA_Gb Cuts at: 2234 2234 Size: 6274 PstI C_TGCA'G Cuts at: 830 2244 2424 3531 3626 830 Size: 1414 180 1107 95 3478 Fragments arranged by size: 3478 1414 1107 180 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6274 PvuII CAG'CTG Cuts at: 2666 3103 2666 Size: 437 5837 SacI G_AGCT'C Cuts at: 2235 2682 2748 3003 2235 Size: 447 66 255 5506 Fragments arranged by size: 5506 447 255 66 SalI G'TCGA_C Cuts at: 1936 3616 1936 Size: 1680 4594 SanDI GG'GwC_CC Cuts at: 2201 2201 Size: 6274 SbfI CC_TGCA'GG Cuts at: 2424 3626 2424 Size: 1202 5072 ScaI AGT'ACT Cuts at: 2984 4522 6234 2984 Size: 1538 1712 3024 Fragments arranged by size: 3024 1712 1538 SfcI C'TryA_G Cuts at: 826 1045 2240 2420 2452 3527 3622 4757 5438 Size: 219 1195 180 32 1075 95 1135 681 Cuts at: 5438 5629 826 Size: 191 1471 Fragments arranged by size: 1471 1195 1135 1075 681 219 191 180 95 32 SfiI GGCCn_nnn'nGGCC Cuts at: 2093 2093 Size: 6274 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6274 SmaI CCC'GGG Cuts at: 1872 3601 1872 Size: 1729 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2108 2213 2234 4378 5279 5541 Size: 197 926 165 105 21 2144 901 262 Cuts at: 5541 820 Size: 1553 Fragments arranged by size: 2144 1553 926 901 262 197 165 105 21 SnaBI TAC'GTA Cuts at: 493 4065 493 Size: 3572 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6274 SphI G_CATG'C Cuts at: 1132 3632 1132 Size: 2500 3774 SspI AAT'ATT Cuts at: 5 52 4198 5 Size: 47 4146 2081 Fragments arranged by size: 4146 2081 47 StuI AGG'CCT Cuts at: 1517 2096 1517 Size: 579 5695 StyI C'CwwG_G Cuts at: 513 1065 3024 3156 3713 513 Size: 552 1959 132 557 3074 Fragments arranged by size: 3074 1959 557 552 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3217 4301 4318 4471 4656 5101 325 Size: 1255 1637 1084 17 153 185 445 1498 Fragments arranged by size: 1637 1498 1255 1084 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2922 2982 4520 Size: 274 80 33 51 1443 945 60 1538 Cuts at: 4520 6232 96 Size: 1712 138 Fragments arranged by size: 1712 1538 1443 945 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2193 2225 3005 3037 6234 6266 2193 Size: 32 780 32 3197 32 2201 Fragments arranged by size: 3197 2201 780 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3164 1316 Size: 1848 4426 XbaI T'CTAG_A Cuts at: 3610 3610 Size: 6274 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3031 3031 Size: 6274 XhoI C'TCGA_G Cuts at: 1943 2234 1943 Size: 291 5983 XmaI C'CCGG_G Cuts at: 1870 3599 1870 Size: 1729 4545 XmnI GAAnn'nnTTC Cuts at: 3542 4403 3542 Size: 861 5413 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4082 276 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacII SapI SexAI SfoI SrfI SwaI