(Circular) (Six-base) MAPSORT of: pFN21AB5126.seq Check: 8210 from: 1 to: 5731 pFN21AB5126 5731 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2464 2464 Size: 5731 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3541 278 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2390 3074 1937 Size: 453 684 4594 Fragments arranged by size: 4594 684 453 Acc65I G'GTAC_C Cuts at: 2109 3052 2109 Size: 943 4788 AclI AA'CG_TT Cuts at: 1563 3858 4231 1563 Size: 2295 373 3063 Fragments arranged by size: 3063 2295 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3794 5178 5446 1632 Size: 2162 1384 268 1917 Fragments arranged by size: 2162 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5534 AflIII A'CryG_T Cuts at: 1203 4630 5607 1203 Size: 3427 977 1327 Fragments arranged by size: 3427 1327 977 AgeI A'CCGG_T Cuts at: 2112 4562 2112 Size: 2450 3281 AhdI GACnn_n'nnGTC Cuts at: 2386 4460 2386 Size: 2074 3657 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5731 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5731 AlwNI CAG_nnn'CTG Cuts at: 2309 3008 3474 5046 2309 Size: 699 466 1572 2994 Fragments arranged by size: 2994 1572 699 466 ApaLI G'TGCA_C Cuts at: 3788 4944 3788 Size: 1156 4575 ApoI r'AATT_y Cuts at: 1493 2577 3040 3266 3418 5527 1493 Size: 1084 463 226 152 2109 1697 Fragments arranged by size: 2109 1697 1084 463 226 152 AseI AT'TA_AT Cuts at: 160 1032 4285 160 Size: 872 3253 1606 Fragments arranged by size: 3253 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 AvaI C'yCGr_G Cuts at: 1870 1943 2046 2135 3056 1870 Size: 73 103 89 921 4545 Fragments arranged by size: 4545 921 103 89 73 BamHI G'GATC_C Cuts at: 3061 3428 3061 Size: 367 5364 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2109 3052 4507 618 Size: 325 217 627 322 943 1455 1842 Fragments arranged by size: 1842 1455 943 627 325 322 217 BanII G_rGCy'C Cuts at: 1391 2411 2500 2756 3050 1391 Size: 1020 89 256 294 4072 Fragments arranged by size: 4072 1020 294 256 89 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2838 928 Size: 971 939 3821 Fragments arranged by size: 3821 971 939 BbvCI CC'TCA_GC Cuts at: 2670 2670 Size: 5731 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2288 2322 3922 3956 1903 Size: 34 50 34 267 34 1600 34 3678 Fragments arranged by size: 3678 1600 267 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2584 3623 4833 2584 Size: 1039 1210 3482 Fragments arranged by size: 3482 1210 1039 BclI T'GATC_A Cuts at: 1548 1548 Size: 5731 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4342 136 Size: 107 122 71 631 605 2670 1525 Fragments arranged by size: 2670 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5726 5726 Size: 5731 BlpI GC'TnA_GC Cuts at: 2469 2755 3148 2469 Size: 286 393 5052 Fragments arranged by size: 5052 393 286 Bme1580I G_kGCm'C Cuts at: 1790 2037 2988 3792 4948 1790 Size: 247 951 804 1156 2573 Fragments arranged by size: 2573 1156 951 804 247 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2764 2790 3462 Size: 416 53 83 399 24 1347 26 672 Cuts at: 3462 4420 442 Size: 958 1753 Fragments arranged by size: 1753 1347 958 672 416 399 83 53 26 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2128 2268 4391 1239 Size: 889 140 2123 2579 Fragments arranged by size: 2579 2123 889 140 Bpu10I CC'TnA_GC Cuts at: 2508 2670 2750 2508 Size: 162 80 5489 Fragments arranged by size: 5489 162 80 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2425 2842 3856 4721 5019 2425 Size: 417 1014 865 298 3137 Fragments arranged by size: 3137 1014 865 417 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4394 882 Size: 617 2895 2219 Fragments arranged by size: 2895 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3522 493 Size: 711 2318 2702 Fragments arranged by size: 2702 2318 711 BsaBI GATnn'nnATC Cuts at: 5725 5725 Size: 5731 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3538 3920 4555 275 Size: 53 83 186 936 2005 382 635 1451 Fragments arranged by size: 2005 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2112 4164 4562 4836 4983 5394 2112 Size: 2052 398 274 147 411 2449 Fragments arranged by size: 2449 2052 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5506 Fragments arranged by size: 5506 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2523 1186 Size: 937 400 4394 Fragments arranged by size: 4394 937 400 BseYI C'CCAG_C Cuts at: 2163 2955 3001 4934 5430 2163 Size: 792 46 1933 496 2464 Fragments arranged by size: 2464 1933 792 496 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5158 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3181 3942 4970 1540 Size: 275 186 1180 761 1028 2301 Fragments arranged by size: 2301 1180 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2411 2500 2756 3792 3877 4948 2411 Size: 89 256 1036 85 1071 3194 Fragments arranged by size: 3194 1071 1036 256 89 85 BsmI GAATG_Cn' Cuts at: 3242 3335 3242 Size: 93 5638 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2411 2500 2756 2988 3050 3792 Size: 399 247 374 89 256 232 62 742 Cuts at: 3792 3877 4948 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 374 256 247 232 89 85 62 BspEI T'CCGG_A Cuts at: 5394 5394 Size: 5731 BspHI T'CATG_A Cuts at: 3620 5350 3620 Size: 1730 4001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2464 3022 3086 844 Size: 1620 558 64 3489 Fragments arranged by size: 3489 1620 558 64 BsrBI CCG'CTC Cuts at: 1394 1823 2926 3618 1394 Size: 429 1103 692 3507 Fragments arranged by size: 3507 1103 692 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4226 4400 65 Size: 1183 2978 174 1396 Fragments arranged by size: 2978 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2112 2168 4375 4562 1700 Size: 110 302 56 2207 187 2869 Fragments arranged by size: 2869 2207 302 187 110 56 BsrGI T'GTAC_A Cuts at: 96 2761 96 Size: 2665 3066 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5731 BssSI C'ACGA_G Cuts at: 3791 4803 3791 Size: 1012 4719 BstAPI GCAn_nnn'nTGC Cuts at: 794 2361 794 Size: 1567 4164 BstBI TT'CG_AA Cuts at: 4535 4535 Size: 5731 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5731 BstYI r'GATC_y Cuts at: 1972 3061 3428 3813 3830 5282 5368 5380 5726 Size: 1089 367 385 17 1452 86 12 346 Cuts at: 5726 1972 Size: 1977 Fragments arranged by size: 1977 1452 1089 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5179 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4992 Fragments arranged by size: 4992 683 56 BtsI GCAGTG_nn' Cuts at: 2635 3247 4041 4061 2635 Size: 612 794 20 4305 Fragments arranged by size: 4305 794 612 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5696 DraI TTT'AAA Cuts at: 3035 3035 Size: 5731 DraIII CAC_nnn'GTG Cuts at: 2804 2983 2804 Size: 179 5552 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2966 4738 809 Size: 507 1650 1772 1802 Fragments arranged by size: 1802 1772 1650 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3178 4067 5696 Size: 54 1425 50 275 18 1348 889 1629 Cuts at: 5696 8 Size: 43 Fragments arranged by size: 1629 1425 1348 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3178 1537 Size: 275 1366 4090 Fragments arranged by size: 4090 1366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3661 1353 Size: 2308 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2215 4318 4692 4838 387 Size: 1064 138 626 2103 374 146 1280 Fragments arranged by size: 2103 1280 1064 626 374 146 138 EcoICRI GAG'CTC Cuts at: 2409 2498 2754 2409 Size: 89 256 5386 Fragments arranged by size: 5386 256 89 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2128 2268 3794 4391 5178 5446 1239 Size: 393 496 140 1526 597 787 268 1524 Fragments arranged by size: 1526 1524 787 597 496 393 268 140 EcoO109I rG'GnC_Cy Cuts at: 2139 2191 2461 2139 Size: 52 270 5409 Fragments arranged by size: 5409 270 52 EcoRI G'AATT_C Cuts at: 3040 3418 3040 Size: 378 5353 FspI TGC'GCA Cuts at: 4237 4237 Size: 5731 HaeII r_GCGC'y Cuts at: 1678 2129 2161 4878 1678 Size: 451 32 2717 2531 Fragments arranged by size: 2717 2531 451 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2372 2404 4372 4404 4446 Size: 32 135 32 800 32 1968 32 42 Cuts at: 4446 4478 1373 Size: 32 2626 Fragments arranged by size: 2626 1968 800 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3075 3321 669 Size: 988 281 1137 246 3079 Fragments arranged by size: 3079 1137 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5731 HpaI GTT'AAC Cuts at: 3321 3321 Size: 5731 KpnI G_GTAC'C Cuts at: 2113 3056 2113 Size: 943 4788 MfeI C'AATT_G Cuts at: 3330 3330 Size: 5731 MluI A'CGCG_T Cuts at: 5607 5607 Size: 5731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2076 4845 5029 1305 Size: 771 2769 184 2007 Fragments arranged by size: 2769 2007 771 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3909 Fragments arranged by size: 3909 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3689 4048 4207 518 Size: 648 2523 359 159 2042 Fragments arranged by size: 2523 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2598 2759 3147 3824 4972 5217 1606 Size: 992 161 388 677 1148 245 2120 Fragments arranged by size: 2120 1148 992 677 388 245 161 NaeI GCC'GGC Cuts at: 2170 2170 Size: 5731 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5179 NdeI CA'TA_TG Cuts at: 387 387 Size: 5731 NgoMIV G'CCGG_C Cuts at: 2168 2168 Size: 5731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5731 NotI GC'GGCC_GC Cuts at: 3178 3178 Size: 5731 NspI r_CATG'y Cuts at: 1132 1229 3089 4634 1132 Size: 97 1860 1545 2229 Fragments arranged by size: 2229 1860 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5731 PciI A'CATG_T Cuts at: 4630 4630 Size: 5731 PflMI CCAn_nnn'nTGG Cuts at: 1108 3008 1108 Size: 1900 3831 PmeI GTTT'AAAC Cuts at: 3035 3035 Size: 5731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3800 3832 5313 5345 3800 Size: 32 1481 32 4186 Fragments arranged by size: 4186 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2191 2461 2191 Size: 270 5461 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5731 PsiI TTA'TAA Cuts at: 3301 3301 Size: 5731 PstI C_TGCA'G Cuts at: 830 2630 3083 830 Size: 1800 453 3478 Fragments arranged by size: 3478 1800 453 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5731 PvuII CAG'CTG Cuts at: 2759 2759 Size: 5731 RsrII CG'GwC_CG Cuts at: 2115 2115 Size: 5731 SacI G_AGCT'C Cuts at: 2411 2500 2756 2411 Size: 89 256 5386 Fragments arranged by size: 5386 256 89 SalI G'TCGA_C Cuts at: 1936 3073 1936 Size: 1137 4594 SbfI CC_TGCA'GG Cuts at: 3083 3083 Size: 5731 ScaI AGT'ACT Cuts at: 3979 5691 3979 Size: 1712 4019 SfcI C'TryA_G Cuts at: 826 1045 2391 2626 3079 4214 4895 5086 826 Size: 219 1346 235 453 1135 681 191 1471 Fragments arranged by size: 1471 1346 1135 681 453 235 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5731 SmaI CCC'GGG Cuts at: 1872 2048 3058 1872 Size: 176 1010 4545 Fragments arranged by size: 4545 1010 176 SmlI C'TyrA_G Cuts at: 820 1017 1943 2404 2857 3835 4736 4998 820 Size: 197 926 461 453 978 901 262 1553 Fragments arranged by size: 1553 978 926 901 461 453 262 197 SnaBI TAC'GTA Cuts at: 493 3522 493 Size: 3029 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5731 SphI G_CATG'C Cuts at: 1132 3089 1132 Size: 1957 3774 SspI AAT'ATT Cuts at: 5 52 3655 5 Size: 47 3603 2081 Fragments arranged by size: 3603 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5731 StyI C'CwwG_G Cuts at: 513 1065 3170 513 Size: 552 2105 3074 Fragments arranged by size: 3074 2105 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3758 3775 3928 4113 4558 325 Size: 1255 2178 17 153 185 445 1498 Fragments arranged by size: 2178 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2735 2761 3977 Size: 274 80 33 51 1443 758 26 1216 Cuts at: 3977 5689 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1216 758 274 138 80 51 33 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5691 5723 5691 Size: 32 5699 Tth111I GACn'n_nGTC Cuts at: 1316 2917 1316 Size: 1601 4130 XbaI T'CTAG_A Cuts at: 3067 3067 Size: 5731 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2243 2777 2243 Size: 534 5197 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5731 XmaI C'CCGG_G Cuts at: 1870 2046 3056 1870 Size: 176 1010 4545 Fragments arranged by size: 4545 1010 176 XmnI GAAnn'nnTTC Cuts at: 3860 3860 Size: 5731 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3539 276 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI