Restriction map for
pFN21AE3520
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AseI AsnI Asp700I
AspEI AspI AvrII BalI BamHI
BbrPI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
Bst1107I BstBI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI MaeII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NaeI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I Van91I XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 512 GGTACC G^GTACC
AcyI 757 GGCGCC G[GA]^CG[CT]C
AscI 739 GGCGCGCC GG^CGCGCC
Asp718I 512 GGTACC G^GTACC
AviII 87 TGCGCA TGC^GCA
BbeI 757 GGCGCC GGCGC^C
BbvCI 148 GCTGAGG CCTCAGC(-5/-2)
BcgI 292 CGAGACTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 757 GGCGCC G[GA]^CG[CT]C
BsaWI 347 ACCGGA [AT]^CCGG[AT]
BsgI 300 GTGCAG GTGCAG(16/14)
BsmBI 385 GAGACG CGTCTC(1/5)
BssSI 297 CTCGTG CACGAG(-5/-1)
BstAPI 704 GCAAGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 18 GGTCACC G^GT[ACGT]ACC
BstF5I 563 CATCC GGATG(2/0)
BstPI 18 GGTCACC G^GT[ACGT]ACC
EcoO65I 18 GGTCACC G^GT[ACGT]ACC
FokI 563 CATCC GGATG(9/13)
FspI 87 TGCGCA TGC^GCA
Hin1I 757 GGCGCC G[GA]^CG[CT]C
KasI 757 GGCGCC G^GCGCC
KpnI 512 GGTACC GGTAC^C
KspI 823 CCGCGG CCGC^GG
NarI 757 GGCGCC GG^CGCC
NcoI 81 CCATGG C^CATGG
NspI 134 GCATGC [GA]CATG^[CT]
PleI 295 GACTC GAGTC(4/5)
PstI 851 CTGCAG CTGCA^G
PvuII 367 CAGCTG CAG^CTG
SacII 823 CCGCGG CCGC^GG
SfoI 757 GGCGCC GGC^GCC
SmaI 452 CCCGGG CCC^GGG
SphI 134 GCATGC GCATG^C
StuI 731 AGGCCT AGG^CCT
TaqI 482 TCGA T^CGA
TspRI 833 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 482 TCGA T^CGA
XbaI 381 TCTAGA T^CTAGA
XcmI 72 CCAGCACTTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 452 CCCGGG C^CCGGG
XmaI 452 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 513 GTAC GT^AC
600 GTAC
AspHI 109 GAGCAC G[AT]GC[AT]^C
535 GAGCAC
BanII 307 GAGCCC G[GA]GC[CT]^C
328 GAGCCC
BbsI 190 GAAGAC GAAGAC(2/6)
478 GTCTTC
BfaI 226 CTAG C^TAG
382 CTAG
BpmI 58 CTGGAG CTGGAG(16/14)
634 CTGGAG
BpuAI 190 GAAGAC GAAGAC(2/6)
478 GTCTTC
BsiEI 389 CGGCCG CG[GA][CT]^CG
859 CGGCCG
BsiHKAI 109 GAGCAC G[AT]GC[AT]^C
535 GAGCAC
BspMI 494 GCAGGT ACCTGC(4/8)
596 GCAGGT
BssHII 725 GCGCGC G^CGCGC
740 GCGCGC
BstNI 267 CCAGG CC^[AT]GG
474 CCAGG
DraII 455 GGGTCCT [GA]G^G[ACGT]CC[CT]
816 GGGACCT
EagI 389 CGGCCG C^GGCCG
859 CGGCCG
EarI 460 CTCTTC CTCTTC(1/4)
322 GAAGAG
EclXI 389 CGGCCG C^GGCCG
859 CGGCCG
Eco52I 389 CGGCCG C^GGCCG
859 CGGCCG
EcoO109I 455 GGGTCCT [GA]G^G[ACGT]CC[CT]
816 GGGACCT
EcoRII 267 CCAGG ^CC[AT]GG
474 CCAGG
EcoT14I 81 CCATGG C^C[AT][AT]GG
311 CCAAGG
HaeII 757 GGCGCC [GA]GCGC^[CT]
763 AGCGCC
HgaI 156 GACGC GACGC(5/10)
713 GCGTC
HphI 573 GGTGA GGTGA(8/7)
20 TCACC
Ksp632I 460 CTCTTC CTCTTC(1/4)
322 GAAGAG
MaeI 226 CTAG C^TAG
382 CTAG
MvaI 267 CCAGG CC^[AT]GG
474 CCAGG
PpuMI 455 GGGTCCT [GA]G^G[AT]CC[CT]
816 GGGACCT
RsaI 513 GTAC GT^AC
600 GTAC
SfcI 747 CTACAG C^T[GA][CT]AG
851 CTGCAG
StyI 81 CCATGG C^C[AT][AT]GG
311 CCAAGG
Tsp45I 19 GTCAC ^GT[GC]AC
715 GTCAC
Tsp509I 161 AATT ^AATT
206 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanI BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BssKI BstUI Cac8I
CfoI Cfr13I DdeI DsaI EaeI
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeIII
MboII MnlI MslI MspA1I MspI
MvnI MwoI NciI NlaIII NlaIV
Sau96I ScrFI TfiI TseI