(Circular) (Six-base) MAPSORT of: pFN21AE3520.seq Check: 8855 from: 1 to: 5584 pFN21AE3520 5584 bp With 182 enzymes: SgfI * March 28, 2012 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3394 278 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2927 1937 Size: 990 4594 Acc65I G'GTAC_C Cuts at: 2517 2905 2517 Size: 388 5196 AclI AA'CG_TT Cuts at: 1563 3711 4084 1563 Size: 2148 373 3063 Fragments arranged by size: 3063 2148 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2396 2579 3647 5031 5299 1632 Size: 764 183 1068 1384 268 1917 Fragments arranged by size: 1917 1384 1068 764 268 183 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5387 AflIII A'CryG_T Cuts at: 1203 4483 5460 1203 Size: 3280 977 1327 Fragments arranged by size: 3280 1327 977 AgeI A'CCGG_T Cuts at: 4415 4415 Size: 5584 AhdI GACnn_n'nnGTC Cuts at: 4313 4313 Size: 5584 AleI CACnn'nnGTG Cuts at: 1166 2576 1166 Size: 1410 4174 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5584 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2486 2518 2486 Size: 32 5552 AlwNI CAG_nnn'CTG Cuts at: 3327 4899 3327 Size: 1572 4012 ApaLI G'TGCA_C Cuts at: 3641 4797 3641 Size: 1156 4428 ApoI r'AATT_y Cuts at: 1493 2893 3119 3271 5380 1493 Size: 1400 226 152 2109 1697 Fragments arranged by size: 2109 1697 1400 226 152 AscI GG'CGCG_CC Cuts at: 2745 2745 Size: 5584 AseI AT'TA_AT Cuts at: 160 1032 4138 160 Size: 872 3106 1606 Fragments arranged by size: 3106 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 AvaI C'yCGr_G Cuts at: 1870 1943 2336 2457 2531 2909 1870 Size: 73 393 121 74 378 4545 Fragments arranged by size: 4545 393 378 121 74 73 BamHI G'GATC_C Cuts at: 2914 3281 2914 Size: 367 5217 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2517 2527 2762 2905 4360 Size: 325 217 627 730 10 235 143 1455 Cuts at: 4360 618 Size: 1842 Fragments arranged by size: 1842 1455 730 627 325 235 217 143 10 BanII G_rGCy'C Cuts at: 1391 2316 2337 2903 1391 Size: 925 21 566 4072 Fragments arranged by size: 4072 925 566 21 BbeI G_GCGC'C Cuts at: 2766 2766 Size: 5584 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2202 2476 928 Size: 971 303 274 4036 Fragments arranged by size: 4036 971 303 274 BbvCI CC'TCA_GC Cuts at: 2154 2154 Size: 5584 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2286 2320 3775 3809 1903 Size: 34 349 34 1455 34 3678 Fragments arranged by size: 3678 1455 349 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3476 4686 3476 Size: 1210 4374 BclI T'GATC_A Cuts at: 1548 1548 Size: 5584 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4195 136 Size: 107 122 71 631 605 2523 1525 Fragments arranged by size: 2523 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5579 5579 Size: 5584 BlpI GC'TnA_GC Cuts at: 3001 3001 Size: 5584 Bme1580I G_kGCm'C Cuts at: 1790 3645 4801 1790 Size: 1855 1156 2573 Fragments arranged by size: 2573 1855 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5584 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3315 4273 442 Size: 416 53 83 399 24 1898 958 1753 Fragments arranged by size: 1898 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5584 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2660 4244 1239 Size: 845 576 1584 2579 Fragments arranged by size: 2579 1584 845 576 Bpu10I CC'TnA_GC Cuts at: 2154 2154 Size: 5584 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3709 4574 4872 3709 Size: 865 298 4421 Fragments arranged by size: 4421 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4247 882 Size: 617 2748 2219 Fragments arranged by size: 2748 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3375 493 Size: 711 2171 2702 Fragments arranged by size: 2702 2171 711 BsaBI GATnn'nnATC Cuts at: 5578 5578 Size: 5584 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2763 3391 3773 4408 Size: 53 83 186 936 1230 628 382 635 Cuts at: 4408 275 Size: 1451 Fragments arranged by size: 1451 1230 936 635 628 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2352 4017 4415 4689 4836 5247 2352 Size: 1665 398 274 147 411 2689 Fragments arranged by size: 2689 1665 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5359 Fragments arranged by size: 5359 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2198 2345 2432 1186 Size: 1012 147 87 4338 Fragments arranged by size: 4338 1012 147 87 BseYI C'CCAG_C Cuts at: 2726 4787 5283 2726 Size: 2061 496 3027 Fragments arranged by size: 3027 2061 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2326 1163 Size: 573 590 4421 Fragments arranged by size: 4421 590 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2397 2867 3034 3795 4823 1540 Size: 275 186 396 470 167 761 1028 2301 Fragments arranged by size: 2301 1028 761 470 396 275 186 167 BsiHKAI G_wGCw'C Cuts at: 2118 2544 3645 3730 4801 2118 Size: 426 1101 85 1071 2901 Fragments arranged by size: 2901 1101 1071 426 85 BsmI GAATG_Cn' Cuts at: 3095 3188 3095 Size: 93 5491 BsmBI CGTCTCn'nnnn_ Cuts at: 2384 2384 Size: 5584 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2316 2337 2544 2903 3645 3730 Size: 399 328 198 21 207 359 742 85 Cuts at: 3730 4801 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 359 328 207 198 85 21 BspEI T'CCGG_A Cuts at: 5247 5247 Size: 5584 BspHI T'CATG_A Cuts at: 3473 5203 3473 Size: 1730 3854 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2490 2592 2939 844 Size: 1646 102 347 3489 Fragments arranged by size: 3489 1646 347 102 BsrBI CCG'CTC Cuts at: 1394 1823 3471 1394 Size: 429 1648 3507 Fragments arranged by size: 3507 1648 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4079 4253 65 Size: 1183 2831 174 1396 Fragments arranged by size: 2831 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4228 4415 1700 Size: 110 2418 187 2869 Fragments arranged by size: 2869 2418 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5584 BssHII G'CGCG_C Cuts at: 1925 2730 2745 1925 Size: 805 15 4764 Fragments arranged by size: 4764 805 15 BssSI C'ACGA_G Cuts at: 2302 3644 4656 2302 Size: 1342 1012 3230 Fragments arranged by size: 3230 1342 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2715 794 Size: 1921 3663 BstBI TT'CG_AA Cuts at: 4388 4388 Size: 5584 BstEII G'GTnAC_C Cuts at: 1184 2023 1184 Size: 839 4745 BstYI r'GATC_y Cuts at: 1972 2914 3281 3666 3683 5135 5221 5233 5579 Size: 942 367 385 17 1452 86 12 346 Cuts at: 5579 1972 Size: 1977 Fragments arranged by size: 1977 1452 942 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2086 2248 2828 513 Size: 552 1021 162 580 3269 Fragments arranged by size: 3269 1021 580 552 162 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4845 Fragments arranged by size: 4845 683 56 BtsI GCAGTG_nn' Cuts at: 2837 3100 3894 3914 2837 Size: 263 794 20 4507 Fragments arranged by size: 4507 794 263 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5584 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5549 DraI TTT'AAA Cuts at: 2888 2888 Size: 5584 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4591 809 Size: 507 3275 1802 Fragments arranged by size: 3275 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2245 2394 Size: 54 1425 50 275 18 315 100 149 Cuts at: 2394 2864 3031 3920 5549 8 Size: 470 167 889 1629 43 Fragments arranged by size: 1629 1425 889 470 315 275 167 149 100 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2394 2864 3031 1537 Size: 275 582 470 167 4090 Fragments arranged by size: 4090 582 470 275 167 EarI CTCTTCn'nnn_ Cuts at: 1353 2322 2471 3514 1353 Size: 969 149 1043 3423 Fragments arranged by size: 3423 1043 969 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2120 2429 4171 4545 4691 387 Size: 1064 138 531 309 1742 374 146 1280 Fragments arranged by size: 1742 1280 1064 531 374 309 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2396 2579 2660 3647 4244 5031 Size: 393 452 312 183 81 987 597 787 Cuts at: 5031 5299 1239 Size: 268 1524 Fragments arranged by size: 1524 987 787 597 452 393 312 268 183 81 EcoO109I rG'GnC_Cy Cuts at: 2461 2822 2461 Size: 361 5223 EcoRI G'AATT_C Cuts at: 2893 3271 2893 Size: 378 5206 FspI TGC'GCA Cuts at: 2094 4090 2094 Size: 1996 3588 FspAI rTGC'GCAy Cuts at: 2094 2094 Size: 5584 HaeII r_GCGC'y Cuts at: 1678 2766 2772 4731 1678 Size: 1088 6 1959 2531 Fragments arranged by size: 2531 1959 1088 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2198 2230 4225 4257 4299 Size: 32 135 32 626 32 1995 32 42 Cuts at: 4299 4331 1373 Size: 32 2626 Fragments arranged by size: 2626 1995 626 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2928 3174 669 Size: 988 281 990 246 3079 Fragments arranged by size: 3079 990 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5584 HpaI GTT'AAC Cuts at: 3174 3174 Size: 5584 KasI G'GCGC_C Cuts at: 2762 2762 Size: 5584 KpnI G_GTAC'C Cuts at: 2521 2909 2521 Size: 388 5196 MfeI C'AATT_G Cuts at: 3183 3183 Size: 5584 MluI A'CGCG_T Cuts at: 5460 5460 Size: 5584 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4698 4882 1305 Size: 3393 184 2007 Fragments arranged by size: 3393 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3762 Fragments arranged by size: 3762 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2085 2576 2842 3542 3901 4060 518 Size: 648 919 491 266 700 359 159 2042 Fragments arranged by size: 2042 919 700 648 491 359 266 159 MspA1I CmG'CkG Cuts at: 1606 2153 2374 2515 2623 2830 3000 3677 4825 Size: 547 221 141 108 207 170 677 1148 Cuts at: 4825 5070 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 547 245 221 207 170 141 108 NarI GG'CG_CC Cuts at: 2763 2763 Size: 5584 NcoI C'CATG_G Cuts at: 513 1065 2086 513 Size: 552 1021 4011 Fragments arranged by size: 4011 1021 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5584 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5584 NotI GC'GGCC_GC Cuts at: 3031 3031 Size: 5584 NspI r_CATG'y Cuts at: 1132 1229 2143 2942 4487 1132 Size: 97 914 799 1545 2229 Fragments arranged by size: 2229 1545 914 799 97 PasI CC'CwG_GG Cuts at: 1348 2272 1348 Size: 924 4660 PciI A'CATG_T Cuts at: 4483 4483 Size: 5584 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5584 PmeI GTTT'AAAC Cuts at: 2888 2888 Size: 5584 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3653 3685 5166 5198 3653 Size: 32 1481 32 4039 Fragments arranged by size: 4039 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2461 2822 2461 Size: 361 5223 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5584 PsiI TTA'TAA Cuts at: 3154 3154 Size: 5584 PstI C_TGCA'G Cuts at: 830 2860 2936 830 Size: 2030 76 3478 Fragments arranged by size: 3478 2030 76 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5584 PvuII CAG'CTG Cuts at: 2374 2374 Size: 5584 SacII CC_GC'GG Cuts at: 2831 2831 Size: 5584 SalI G'TCGA_C Cuts at: 1936 2926 1936 Size: 990 4594 SbfI CC_TGCA'GG Cuts at: 2936 2936 Size: 5584 ScaI AGT'ACT Cuts at: 3832 5544 3832 Size: 1712 3872 SfcI C'TryA_G Cuts at: 826 1045 2752 2856 2932 4067 4748 4939 826 Size: 219 1707 104 76 1135 681 191 1471 Fragments arranged by size: 1707 1471 1135 681 219 191 104 76 SfoI GGC'GCC Cuts at: 2764 2764 Size: 5584 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5584 SmaI CCC'GGG Cuts at: 1872 2459 2911 1872 Size: 587 452 4545 Fragments arranged by size: 4545 587 452 SmlI C'TyrA_G Cuts at: 820 1017 1943 3688 4589 4851 820 Size: 197 926 1745 901 262 1553 Fragments arranged by size: 1745 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3375 493 Size: 2882 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5584 SphI G_CATG'C Cuts at: 1132 2143 2942 1132 Size: 1011 799 3774 Fragments arranged by size: 3774 1011 799 SspI AAT'ATT Cuts at: 5 52 3508 5 Size: 47 3456 2081 Fragments arranged by size: 3456 2081 47 StuI AGG'CCT Cuts at: 1517 2738 1517 Size: 1221 4363 StyI C'CwwG_G Cuts at: 513 1065 2086 2316 3023 513 Size: 552 1021 230 707 3074 Fragments arranged by size: 3074 1021 707 552 230 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3611 3628 3781 3966 4411 325 Size: 1255 2031 17 153 185 445 1498 Fragments arranged by size: 2031 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3830 5542 96 Size: 274 80 33 51 1443 1853 1712 138 Fragments arranged by size: 1853 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5544 5576 5544 Size: 32 5552 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5584 XbaI T'CTAG_A Cuts at: 2386 2920 2386 Size: 534 5050 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2084 2084 Size: 5584 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5584 XmaI C'CCGG_G Cuts at: 1870 2457 2909 1870 Size: 587 452 4545 Fragments arranged by size: 4545 587 452 XmnI GAAnn'nnTTC Cuts at: 3713 3713 Size: 5584 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3392 276 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI