(Linear) (Six-base) MAPSORT of: KIEE3520.seq Check: 3834 from: 1 to: 879 KIEE3520.orf With 182 enzymes: SgfI * March 22, 2012 16:36 .. Acc65I G'GTAC_C Cuts at: 0 512 879 Size: 512 367 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 391 574 879 Size: 391 183 305 Fragments arranged by size: 391 305 183 AleI CACnn'nnGTG Cuts at: 0 571 879 Size: 571 308 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 481 513 879 Size: 481 32 366 Fragments arranged by size: 481 366 32 AscI GG'CGCG_CC Cuts at: 0 740 879 Size: 740 139 AvaI C'yCGr_G Cuts at: 0 331 452 526 879 Size: 331 121 74 353 Fragments arranged by size: 353 331 121 74 BanI G'GyrC_C Cuts at: 0 512 522 757 879 Size: 512 10 235 122 Fragments arranged by size: 512 235 122 10 BanII G_rGCy'C Cuts at: 0 311 332 879 Size: 311 21 547 Fragments arranged by size: 547 311 21 BbeI G_GCGC'C Cuts at: 0 761 879 Size: 761 118 BbsI GAAGACnn'nnnn_ Cuts at: 0 197 471 879 Size: 197 274 408 Fragments arranged by size: 408 274 197 BbvCI CC'TCA_GC Cuts at: 0 149 879 Size: 149 730 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 281 315 879 Size: 281 34 564 Fragments arranged by size: 564 281 34 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 655 879 Size: 79 576 224 Fragments arranged by size: 576 224 79 Bpu10I CC'TnA_GC Cuts at: 0 149 879 Size: 149 730 BsaHI Gr'CG_yC Cuts at: 0 758 879 Size: 758 121 BsaWI w'CCGG_w Cuts at: 0 347 879 Size: 347 532 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 193 340 427 879 Size: 193 147 87 452 Fragments arranged by size: 452 193 147 87 BseYI C'CCAG_C Cuts at: 0 721 879 Size: 721 158 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 321 879 Size: 321 558 BsiEI CG_ry'CG Cuts at: 0 392 862 879 Size: 392 470 17 Fragments arranged by size: 470 392 17 BsiHKAI G_wGCw'C Cuts at: 0 113 539 879 Size: 113 426 340 Fragments arranged by size: 426 340 113 BsmBI CGTCTCn'nnnn_ Cuts at: 0 379 879 Size: 379 500 Bsp1286I G_dGCh'C Cuts at: 0 113 311 332 539 879 Size: 113 198 21 207 340 Fragments arranged by size: 340 207 198 113 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 485 587 879 Size: 485 102 292 Fragments arranged by size: 485 292 102 BssHII G'CGCG_C Cuts at: 0 725 740 879 Size: 725 15 139 Fragments arranged by size: 725 139 15 BssSI C'ACGA_G Cuts at: 0 297 879 Size: 297 582 BstAPI GCAn_nnn'nTGC Cuts at: 0 710 879 Size: 710 169 BstEII G'GTnAC_C Cuts at: 0 18 879 Size: 18 861 BtgI C'CryG_G Cuts at: 0 81 243 823 879 Size: 81 162 580 56 Fragments arranged by size: 580 162 81 56 BtsI GCAGTG_nn' Cuts at: 0 832 879 Size: 832 47 EaeI y'GGCC_r Cuts at: 0 140 240 389 859 879 Size: 140 100 149 470 20 Fragments arranged by size: 470 149 140 100 20 EagI C'GGCC_G Cuts at: 0 389 859 879 Size: 389 470 20 Fragments arranged by size: 470 389 20 EarI CTCTTCn'nnn_ Cuts at: 0 317 466 879 Size: 317 149 413 Fragments arranged by size: 413 317 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 115 424 879 Size: 115 309 455 Fragments arranged by size: 455 309 115 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 391 574 655 879 Size: 79 312 183 81 224 Fragments arranged by size: 312 224 183 81 79 EcoO109I rG'GnC_Cy Cuts at: 0 456 817 879 Size: 456 361 62 Fragments arranged by size: 456 361 62 FspI TGC'GCA Cuts at: 0 89 879 Size: 89 790 FspAI rTGC'GCAy Cuts at: 0 89 879 Size: 89 790 HaeII r_GCGC'y Cuts at: 0 761 767 879 Size: 761 6 112 Fragments arranged by size: 761 112 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 193 225 879 Size: 193 32 654 Fragments arranged by size: 654 193 32 KasI G'GCGC_C Cuts at: 0 757 879 Size: 757 122 KpnI G_GTAC'C Cuts at: 0 516 879 Size: 516 363 MslI CAynn'nnrTG Cuts at: 0 80 571 837 879 Size: 80 491 266 42 Fragments arranged by size: 491 266 80 42 MspA1I CmG'CkG Cuts at: 0 148 369 510 618 825 879 Size: 148 221 141 108 207 54 Fragments arranged by size: 221 207 148 141 108 54 NarI GG'CG_CC Cuts at: 0 758 879 Size: 758 121 NcoI C'CATG_G Cuts at: 0 81 879 Size: 81 798 NspI r_CATG'y Cuts at: 0 138 879 Size: 138 741 PasI CC'CwG_GG Cuts at: 0 267 879 Size: 267 612 PpuMI rG'GwC_Cy Cuts at: 0 456 817 879 Size: 456 361 62 Fragments arranged by size: 456 361 62 PstI C_TGCA'G Cuts at: 0 855 879 Size: 855 24 PvuII CAG'CTG Cuts at: 0 369 879 Size: 369 510 SacII CC_GC'GG Cuts at: 0 826 879 Size: 826 53 SfcI C'TryA_G Cuts at: 0 747 851 879 Size: 747 104 28 Fragments arranged by size: 747 104 28 SfoI GGC'GCC Cuts at: 0 759 879 Size: 759 120 SmaI CCC'GGG Cuts at: 0 454 879 Size: 454 425 SphI G_CATG'C Cuts at: 0 138 879 Size: 138 741 StuI AGG'CCT Cuts at: 0 733 879 Size: 733 146 StyI C'CwwG_G Cuts at: 0 81 311 879 Size: 81 230 568 Fragments arranged by size: 568 230 81 XbaI T'CTAG_A Cuts at: 0 381 879 Size: 381 498 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 79 879 Size: 79 800 XmaI C'CCGG_G Cuts at: 0 452 879 Size: 452 427 Enzymes that do cut: Acc65I AcuI AleI AloI AscI AvaI BanI BanII BbeI BbsI BbvCI BcgI BpmI Bpu10I BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BssHII BssSI BstAPI BstEII BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I FspI FspAI HaeII Hin4I KasI KpnI MslI MspA1I NarI NcoI NspI PasI PpuMI PstI PvuII SacII SfcI SfoI SmaI SphI StuI StyI XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaXI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI HincII HindIII HpaI MfeI MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TaqII TatI TstI Tth111I XhoI XmnI ZraI