################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MscI MseI MslI MspA1I MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccII 368 CGCG CG^CG AccIII 215 TCCGGA T^CCGGA AclI 685 AACGTT AA^CGTT Aor51HI 419 AGCGCT AGC^GCT BbsI 625 GAAGAC GAAGAC(2/6) BbvCI 694 GCTGAGG CCTCAGC(-5/-2) BfaI 142 CTAG C^TAG BglI 425 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 606 GCTCAGC GC^T[ACGT]AGC Bpu1102I 606 GCTCAGC GC^T[ACGT]AGC BpuAI 625 GAAGAC GAAGAC(2/6) BseAI 215 TCCGGA T^CCGGA BseRI 658 GAGGAG GAGGAG(10/8) BsiEI 455 CGGCCG CG[GA][CT]^CG BspEI 215 TCCGGA T^CCGGA BspMI 360 GCAGGT ACCTGC(4/8) BsrBI 632 CCGCTC CCGCTC(-3/-3) BsrDI 397 CATTGC GCAATG(2/0) BsrFI 389 ACCGGC [GA]^CCGG[CT] BsrI 578 CCAGT ACTGG(1/-1) BstAPI 119 GCAAAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 129 CATCC GGATG(2/0) BstUI 368 CGCG CG^CG CelII 606 GCTCAGC GC^T[ACGT]AGC Cfr10I 389 ACCGGC [GA]^CCGG[CT] DpnII 16 GATC ^GATC DsaI 69 CCATGG C^C[GA][CT]GG EagI 455 CGGCCG C^GGCCG EarI 25 CTCTTC CTCTTC(1/4) EclXI 455 CGGCCG C^GGCCG Eco47III 419 AGCGCT AGC^GCT Eco52I 455 CGGCCG C^GGCCG EcoT14I 69 CCATGG C^C[AT][AT]GG FokI 129 CATCC GGATG(9/13) HgaI 259 GACGC GACGC(5/10) HinfI 310 GACTC G^A[ACGT]TC HphI 180 GGTGA GGTGA(8/7) Ksp632I 25 CTCTTC CTCTTC(1/4) MaeI 142 CTAG C^TAG MaeII 686 ACGT A^CGT MaeIII 274 GTGAC ^GT[ACGT]AC MboI 16 GATC ^GATC MroI 215 TCCGGA T^CCGGA MvnI 368 CGCG CG^CG NcoI 69 CCATGG C^CATGG NdeII 16 GATC ^GATC NheI 141 GCTAGC G^CTAGC NspI 277 ACATGC [GA]CATG^[CT] PleI 310 GACTC GAGTC(4/5) Psp1406I 685 AACGTT AA^CGTT SacI 604 GAGCTC GAGCT^C Sau3AI 16 GATC ^GATC SfaNI 128 GCATC GCATC(5/9) SfcI 168 CTGTAG C^T[GA][CT]AG SmlI 79 CTCAAG C^T[CT][GA]AG StyI 69 CCATGG C^C[AT][AT]GG Tsp45I 274 GTGAC ^GT[GC]AC TspRI 579 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 224 CCATTGGCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BanII 604 GAGCTC G[GA]GC[CT]^C 709 GAGCCC BcnI 453 CCCGG CC^[GC]GG 483 CCGGG BsaWI 134 TCCGGT [AT]^CCGG[AT] 215 TCCGGA BsgI 351 CTGCAC GTGCAG(16/14) 490 CTGCAC DdeI 607 CTCAG C^T[ACGT]AG 695 CTGAG DraII 373 GGGACCC [GA]G^G[ACGT]CC[CT] 409 GGGTCCC EaeI 228 TGGCCG [CT]^GGCC[GA] 455 CGGCCG EcoO109I 373 GGGACCC [GA]G^G[ACGT]CC[CT] 409 GGGTCCC FauI 8 GCGGG CCCGC(4/6) 437 GCGGG HaeII 419 AGCGCT [GA]GCGC^[CT] 518 AGCGCC NciI 453 CCCGG CC^[GC]GG 483 CCGGG PpuMI 373 GGGACCC [GA]G^G[AT]CC[CT] 409 GGGTCCC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AluI AspHI AvaII BanI BbvI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI