(Circular) (Six-base) MAPSORT of: pFN21AE3514.seq Check: 2220 from: 1 to: 5440 pFN21AE3514 5440 bp With 182 enzymes: SgfI * March 28, 2012 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2356 2356 Size: 5440 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3250 278 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2783 1937 Size: 846 4594 Acc65I G'GTAC_C Cuts at: 2761 2761 Size: 5440 AclI AA'CG_TT Cuts at: 1563 2691 3567 3940 1563 Size: 1128 876 373 3063 Fragments arranged by size: 3063 1128 876 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3503 4887 5155 1632 Size: 1871 1384 268 1917 Fragments arranged by size: 1917 1871 1384 268 AfeI AGC'GCT Cuts at: 2426 2426 Size: 5440 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5243 AflIII A'CryG_T Cuts at: 1203 4339 5316 1203 Size: 3136 977 1327 Fragments arranged by size: 3136 1327 977 AgeI A'CCGG_T Cuts at: 4271 4271 Size: 5440 AhdI GACnn_n'nnGTC Cuts at: 4169 4169 Size: 5440 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5440 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5440 AlwNI CAG_nnn'CTG Cuts at: 3183 4755 3183 Size: 1572 3868 ApaLI G'TGCA_C Cuts at: 3497 4653 3497 Size: 1156 4284 ApoI r'AATT_y Cuts at: 1493 2749 2975 3127 5236 1493 Size: 1256 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 226 152 AseI AT'TA_AT Cuts at: 160 1032 3994 160 Size: 872 2962 1606 Fragments arranged by size: 2962 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 AvaI C'yCGr_G Cuts at: 1870 1943 2765 1870 Size: 73 822 4545 Fragments arranged by size: 4545 822 73 BamHI G'GATC_C Cuts at: 2770 3137 2770 Size: 367 5073 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2307 2368 2651 2761 4216 Size: 325 217 627 520 61 283 110 1455 Cuts at: 4216 618 Size: 1842 Fragments arranged by size: 1842 1455 627 520 325 283 217 110 61 BanII G_rGCy'C Cuts at: 1391 2613 2718 2759 1391 Size: 1222 105 41 4072 Fragments arranged by size: 4072 1222 105 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2637 928 Size: 971 738 3731 Fragments arranged by size: 3731 971 738 BbvCI CC'TCA_GC Cuts at: 2700 2700 Size: 5440 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3631 3665 1903 Size: 34 1694 34 3678 Fragments arranged by size: 3678 1694 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3332 4542 3332 Size: 1210 4230 BclI T'GATC_A Cuts at: 1548 1548 Size: 5440 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2436 4051 136 Size: 107 122 71 631 605 764 1615 1525 Fragments arranged by size: 1615 1525 764 631 605 122 107 71 BglII A'GATC_T Cuts at: 5435 5435 Size: 5440 BlpI GC'TnA_GC Cuts at: 2612 2857 2612 Size: 245 5195 Bme1580I G_kGCm'C Cuts at: 1790 2310 2656 3501 4657 1790 Size: 520 346 845 1156 2573 Fragments arranged by size: 2573 1156 845 520 346 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5440 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3171 4129 442 Size: 416 53 83 399 24 1754 958 1753 Fragments arranged by size: 1754 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2150 1056 Size: 1094 4346 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2527 2645 2666 4100 1239 Size: 1288 118 21 1434 2579 Fragments arranged by size: 2579 1434 1288 118 21 Bpu10I CC'TnA_GC Cuts at: 2700 2700 Size: 5440 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2069 3565 4430 4728 2069 Size: 1496 865 298 2781 Fragments arranged by size: 2781 1496 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4103 882 Size: 617 2604 2219 Fragments arranged by size: 2604 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3231 493 Size: 711 2027 2702 Fragments arranged by size: 2702 2027 711 BsaBI GATnn'nnATC Cuts at: 5434 5434 Size: 5440 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3247 3629 4264 275 Size: 53 83 186 936 1714 382 635 1451 Fragments arranged by size: 1714 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2139 2220 3873 4271 4545 4692 5103 2139 Size: 81 1653 398 274 147 411 2476 Fragments arranged by size: 2476 1653 411 398 274 147 81 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5215 Fragments arranged by size: 5215 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2678 1186 Size: 1492 3948 BseYI C'CCAG_C Cuts at: 2418 4643 5139 2418 Size: 2225 496 2719 Fragments arranged by size: 2719 2225 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2341 2480 1163 Size: 573 605 139 4123 Fragments arranged by size: 4123 605 573 139 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2463 2890 3651 4679 1540 Size: 275 186 462 427 761 1028 2301 Fragments arranged by size: 2301 1028 761 462 427 275 186 BsiHKAI G_wGCw'C Cuts at: 2066 2394 2473 2613 3501 3586 4657 2066 Size: 328 79 140 888 85 1071 2849 Fragments arranged by size: 2849 1071 888 328 140 85 79 BsmI GAATG_Cn' Cuts at: 2951 3044 2951 Size: 93 5347 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2310 2394 2473 2613 2656 2718 Size: 399 276 244 84 79 140 43 62 Cuts at: 2718 2759 3501 3586 4657 1391 Size: 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 276 244 140 85 84 79 62 43 41 BspEI T'CCGG_A Cuts at: 2220 5103 2220 Size: 2883 2557 BspHI T'CATG_A Cuts at: 3329 5059 3329 Size: 1730 3710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2356 2795 844 Size: 1512 439 3489 Fragments arranged by size: 3489 1512 439 BsrBI CCG'CTC Cuts at: 1394 1823 2639 3327 1394 Size: 429 816 688 3507 Fragments arranged by size: 3507 816 688 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2401 3935 4109 65 Size: 1183 1153 1534 174 1396 Fragments arranged by size: 1534 1396 1183 1153 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2394 4084 4271 1700 Size: 110 584 1690 187 2869 Fragments arranged by size: 2869 1690 584 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5440 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5440 BssSI C'ACGA_G Cuts at: 3500 4512 3500 Size: 1012 4428 BstAPI GCAn_nnn'nTGC Cuts at: 794 2130 794 Size: 1336 4104 BstBI TT'CG_AA Cuts at: 4244 4244 Size: 5440 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5440 BstYI r'GATC_y Cuts at: 1972 2770 3137 3522 3539 4991 5077 5089 5435 Size: 798 367 385 17 1452 86 12 346 Cuts at: 5435 1972 Size: 1977 Fragments arranged by size: 1977 1452 798 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2074 513 Size: 552 939 70 3879 Fragments arranged by size: 3879 939 552 70 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2282 487 Size: 683 56 1056 3645 Fragments arranged by size: 3645 1056 683 56 BtsI GCAGTG_nn' Cuts at: 2956 3750 3770 2956 Size: 794 20 4626 Fragments arranged by size: 4626 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5440 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5405 DraI TTT'AAA Cuts at: 2744 2744 Size: 5440 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4447 809 Size: 507 3131 1802 Fragments arranged by size: 3131 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2233 2460 2887 Size: 54 1425 50 275 18 403 227 427 Cuts at: 2887 3776 5405 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 427 403 275 227 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2460 2887 1537 Size: 275 648 427 4090 Fragments arranged by size: 4090 648 427 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2036 3370 1353 Size: 683 1334 3423 Fragments arranged by size: 3423 1334 683 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2621 4027 4401 4547 387 Size: 1064 138 1032 1406 374 146 1280 Fragments arranged by size: 1406 1280 1064 1032 374 146 138 EcoICRI GAG'CTC Cuts at: 2611 2611 Size: 5440 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2527 2645 2666 3503 4100 4887 5155 Size: 393 895 118 21 837 597 787 268 Cuts at: 5155 1239 Size: 1524 Fragments arranged by size: 1524 895 837 787 597 393 268 118 21 EcoO109I rG'GnC_Cy Cuts at: 2379 2415 2379 Size: 36 5404 EcoRI G'AATT_C Cuts at: 2749 3127 2749 Size: 378 5062 FspI TGC'GCA Cuts at: 3946 3946 Size: 5440 HaeII r_GCGC'y Cuts at: 1678 2428 2527 4587 1678 Size: 750 99 2060 2531 Fragments arranged by size: 2531 2060 750 99 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4081 4113 4155 4187 1373 Size: 32 135 32 2509 32 42 32 2626 Fragments arranged by size: 2626 2509 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2784 3030 669 Size: 988 281 846 246 3079 Fragments arranged by size: 3079 988 846 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5440 HpaI GTT'AAC Cuts at: 3030 3030 Size: 5440 KpnI G_GTAC'C Cuts at: 2765 2765 Size: 5440 MfeI C'AATT_G Cuts at: 3039 3039 Size: 5440 MluI A'CGCG_T Cuts at: 5316 5316 Size: 5440 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4554 4738 1305 Size: 3249 184 2007 Fragments arranged by size: 3249 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3618 Fragments arranged by size: 3618 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3398 3757 3916 518 Size: 648 2232 359 159 2042 Fragments arranged by size: 2232 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2856 3533 4681 4926 1606 Size: 1250 677 1148 245 2120 Fragments arranged by size: 2120 1250 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2074 513 Size: 552 939 70 3879 Fragments arranged by size: 3879 939 552 70 NdeI CA'TA_TG Cuts at: 387 387 Size: 5440 NheI G'CTAG_C Cuts at: 1052 2146 1052 Size: 1094 4346 NotI GC'GGCC_GC Cuts at: 2887 2887 Size: 5440 NspI r_CATG'y Cuts at: 1132 1229 2286 2798 4343 1132 Size: 97 1057 512 1545 2229 Fragments arranged by size: 2229 1545 1057 512 97 PasI CC'CwG_GG Cuts at: 1348 2434 2718 1348 Size: 1086 284 4070 Fragments arranged by size: 4070 1086 284 PciI A'CATG_T Cuts at: 4339 4339 Size: 5440 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5440 PfoI T'CCnGG_A Cuts at: 2622 2622 Size: 5440 PmeI GTTT'AAAC Cuts at: 2744 2744 Size: 5440 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3509 3541 5022 5054 3509 Size: 32 1481 32 3895 Fragments arranged by size: 3895 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2379 2415 2379 Size: 36 5404 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5440 PsiI TTA'TAA Cuts at: 3010 3010 Size: 5440 PstI C_TGCA'G Cuts at: 830 2792 830 Size: 1962 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5440 SacI G_AGCT'C Cuts at: 2613 2613 Size: 5440 SalI G'TCGA_C Cuts at: 1936 2782 1936 Size: 846 4594 SanDI GG'GwC_CC Cuts at: 2379 2415 2379 Size: 36 5404 SbfI CC_TGCA'GG Cuts at: 2792 2792 Size: 5440 ScaI AGT'ACT Cuts at: 3688 5400 3688 Size: 1712 3728 SfcI C'TryA_G Cuts at: 826 1045 2173 2788 3923 4604 4795 826 Size: 219 1128 615 1135 681 191 1471 Fragments arranged by size: 1471 1135 1128 681 615 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5440 SmaI CCC'GGG Cuts at: 1872 2767 1872 Size: 895 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2084 3544 4445 4707 820 Size: 197 926 141 1460 901 262 1553 Fragments arranged by size: 1553 1460 926 901 262 197 141 SnaBI TAC'GTA Cuts at: 493 3231 493 Size: 2738 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5440 SphI G_CATG'C Cuts at: 1132 2798 1132 Size: 1666 3774 SspI AAT'ATT Cuts at: 5 52 3364 5 Size: 47 3312 2081 Fragments arranged by size: 3312 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5440 StyI C'CwwG_G Cuts at: 513 1065 2004 2074 2879 513 Size: 552 939 70 805 3074 Fragments arranged by size: 3074 939 805 552 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 3467 3484 3637 3822 4267 325 Size: 1255 417 1470 17 153 185 445 1498 Fragments arranged by size: 1498 1470 1255 445 417 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3686 5398 96 Size: 274 80 33 51 1443 1709 1712 138 Fragments arranged by size: 1712 1709 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2234 2266 5400 5432 2234 Size: 32 3134 32 2242 Fragments arranged by size: 3134 2242 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5440 XbaI T'CTAG_A Cuts at: 2776 2776 Size: 5440 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2236 2236 Size: 5440 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5440 XmaI C'CCGG_G Cuts at: 1870 2765 1870 Size: 895 4545 XmnI GAAnn'nnTTC Cuts at: 3569 3569 Size: 5440 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3248 276 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI