Restriction map for
pFN21AB5011
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglII BlnI BsaAI BsaBI
BsaHI BsaI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AspHI 230 GAGCTC G[AT]GC[AT]^C
AviII 194 TGCGCA TGC^GCA
BanII 230 GAGCTC G[GA]GC[CT]^C
BfaI 1067 CTAG C^TAG
BglI 892 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 230 GAGCTC G[AGT]GC[ACT]^C
BsiHKAI 230 GAGCTC G[AT]GC[AT]^C
BsmAI 834 GAGAC GTCTC(1/5)
Bsp1286I 230 GAGCTC G[AGT]GC[ACT]^C
BsrDI 888 CATTGC GCAATG(2/0)
DraII 278 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 278 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 18 CCTTGG C^C[AT][AT]GG
FauI 282 CCCGC CCCGC(4/6)
FspI 194 TGCGCA TGC^GCA
HaeII 177 GGCGCT [GA]GCGC^[CT]
HgaI 1101 GACGC GACGC(5/10)
MaeI 1067 CTAG C^TAG
MaeII 606 ACGT A^CGT
NlaIV 279 GGTCCC GG[ACGT]^[ACGT]CC
NspI 480 GCATGT [GA]CATG^[CT]
PpuMI 278 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 631 CTGCAG CTGCA^G
SacI 230 GAGCTC GAGCT^C
ScaI 509 AGTACT AGT^ACT
SexAI 44 ACCTGGT A^CC[AT]GGT
SfcI 631 CTGCAG C^T[GA][CT]AG
SspI 751 AATATT AAT^ATT
StyI 18 CCTTGG C^C[AT][AT]GG
TaqI 832 TCGA T^CGA
Tsp509I 540 AATT ^AATT
TthHB8I 832 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 56 TCCGGA T^CCGGA
1097 TCCGGA
AfaI 510 GTAC GT^AC
1197 GTAC
AlwI 59 GGATC GGATC(4/5)
351 GGATC
AvaII 93 GGACC G^G[AT]CC
279 GGTCC
BalI 21 TGGCCA TGG^CCA
884 TGGCCA
BlpI 105 GCTCAGC GC^T[ACGT]AGC
739 GCTAAGC
BpmI 364 CTGGAG CTGGAG(16/14)
1241 CTGGAG
Bpu1102I 105 GCTCAGC GC^T[ACGT]AGC
739 GCTAAGC
BsaJI 18 CCTTGG C^C[ACGT][ACGT]GG
1214 CCCAGG
BsaWI 56 TCCGGA [AT]^CCGG[AT]
1097 TCCGGA
BseAI 56 TCCGGA T^CCGGA
1097 TCCGGA
BsgI 799 CTGCAC GTGCAG(16/14)
766 GTGCAG
BsmFI 503 GGGAC GGGAC(10/14)
280 GTCCC
BspEI 56 TCCGGA T^CCGGA
1097 TCCGGA
BspMI 710 GCAGGT ACCTGC(4/8)
921 GCAGGT
BstXI 260 CCATCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
611 CCAACTCTATGG
CelII 105 GCTCAGC GC^T[ACGT]AGC
739 GCTAAGC
MluNI 21 TGGCCA TGG^CCA
884 TGGCCA
MroI 56 TCCGGA T^CCGGA
1097 TCCGGA
MscI 21 TGGCCA TGG^CCA
884 TGGCCA
MseI 719 TTAA T^TAA
857 TTAA
MwoI 892 GCCACTTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
997 GCTGTCAAAGC
PflMI 174 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
357 CCAGGTGCTGG
RsaI 510 GTAC GT^AC
1197 GTAC
SfaNI 81 GATGC GCATC(5/9)
979 GATGC
SmlI 388 CTTGAG C^T[CT][GA]AG
695 CTCAAG
Tru9I 719 TTAA T^TAA
857 TTAA
Van91I 174 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
357 CCAGGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwNI BbsI BbvI
BpuAI BsiYI BslI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI NdeII
NlaIII PleI PvuII Sau3AI Sau96I
ScrFI TfiI TseI Tsp45I TspRI
XcmI