(Circular) (Six-base) MAPSORT of: pFN21AB5011.seq Check: 6257 from: 1 to: 5956 pFN21AB5011 5956 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2917 2917 Size: 5956 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3299 1937 Size: 1362 4594 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4019 5403 5671 1632 Size: 2387 1384 268 1917 Fragments arranged by size: 2387 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 3253 4855 5832 1203 Size: 2050 1602 977 1327 Fragments arranged by size: 2050 1602 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5956 AlwNI CAG_nnn'CTG Cuts at: 2185 2368 2779 3205 3699 5271 2185 Size: 183 411 426 494 1572 2870 Fragments arranged by size: 2870 1572 494 426 411 183 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 3265 3491 3643 5752 1493 Size: 1772 226 152 2109 1697 Fragments arranged by size: 2109 1772 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 3281 1870 Size: 73 1338 4545 Fragments arranged by size: 4545 1338 73 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3277 4732 618 Size: 325 217 627 1490 1455 1842 Fragments arranged by size: 1842 1490 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2239 3275 1391 Size: 848 1036 4072 Fragments arranged by size: 4072 1036 848 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2589 2676 3237 928 Size: 971 690 87 561 3647 Fragments arranged by size: 3647 971 690 561 87 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4147 4181 1903 Size: 34 2210 34 3678 Fragments arranged by size: 3678 2210 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2903 4567 136 Size: 107 122 71 631 605 1231 1664 1525 Fragments arranged by size: 1664 1525 1231 631 605 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 2111 2745 3373 2111 Size: 634 628 4694 Fragments arranged by size: 4694 634 628 Bme1580I G_kGCm'C Cuts at: 1790 4017 5173 1790 Size: 2227 1156 2573 Fragments arranged by size: 2573 2227 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3687 4645 442 Size: 416 53 83 399 24 2270 958 1753 Fragments arranged by size: 2270 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2390 3267 4616 1239 Size: 1151 877 1349 2579 Fragments arranged by size: 2579 1349 1151 877 Bpu10I CC'TnA_GC Cuts at: 3191 3191 Size: 5956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2414 2685 4081 4946 5244 2414 Size: 271 1396 865 298 3126 Fragments arranged by size: 3126 1396 865 298 271 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4619 882 Size: 617 3120 2219 Fragments arranged by size: 3120 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3763 4145 4780 275 Size: 53 83 186 936 2230 382 635 1451 Fragments arranged by size: 2230 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2061 3102 4389 4787 5061 5208 5619 2061 Size: 1041 1287 398 274 147 411 2398 Fragments arranged by size: 2398 1287 1041 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3240 3270 1178 Size: 30 165 30 1837 30 3864 Fragments arranged by size: 3864 1837 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5956 BseYI C'CCAG_C Cuts at: 2531 5159 5655 2531 Size: 2628 496 2832 Fragments arranged by size: 2832 2628 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2789 2792 1163 Size: 573 1053 3 4327 Fragments arranged by size: 4327 1053 573 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2239 4017 4102 5173 2239 Size: 1778 85 1071 3022 Fragments arranged by size: 3022 1778 1071 85 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2239 3275 4017 4102 5173 1391 Size: 399 449 1036 742 85 1071 2174 Fragments arranged by size: 2174 1071 1036 742 449 399 85 BspEI T'CCGG_A Cuts at: 2061 3102 5619 2061 Size: 1041 2517 2398 Fragments arranged by size: 2517 2398 1041 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2706 2917 3311 844 Size: 1862 211 394 3489 Fragments arranged by size: 3489 1862 394 211 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2892 4451 4625 65 Size: 1183 1644 1559 174 1396 Fragments arranged by size: 1644 1559 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4600 4787 1700 Size: 110 2790 187 2869 Fragments arranged by size: 2869 2790 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 4016 5028 4016 Size: 1012 4944 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5956 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstXI CCAn_nnnn'nTGG Cuts at: 2272 2623 2272 Size: 351 5605 BstYI r'GATC_y Cuts at: 1972 3286 3653 4038 4055 5507 5593 5605 5951 Size: 1314 367 385 17 1452 86 12 346 Cuts at: 5951 1972 Size: 1977 Fragments arranged by size: 1977 1452 1314 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4465 Fragments arranged by size: 4465 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5217 Fragments arranged by size: 5217 683 56 BtsI GCAGTG_nn' Cuts at: 2214 2780 2801 2949 2973 3472 4266 4286 2214 Size: 566 21 148 24 499 794 20 3884 Fragments arranged by size: 3884 794 566 499 148 24 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2026 2766 2889 Size: 54 1425 50 275 18 196 740 123 Cuts at: 2889 3403 4292 5921 8 Size: 514 889 1629 43 Fragments arranged by size: 1629 1425 889 740 514 275 196 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 2680 3027 3886 1353 Size: 678 649 347 859 3423 Fragments arranged by size: 3423 859 678 649 347 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4543 4917 5063 387 Size: 1064 138 2954 374 146 1280 Fragments arranged by size: 2954 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2237 2237 Size: 5956 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2390 3267 4019 4616 5403 5671 1239 Size: 393 758 877 752 597 787 268 1524 Fragments arranged by size: 1524 877 787 758 752 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2284 2284 Size: 5956 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 2201 4462 2201 Size: 2261 3695 HaeII r_GCGC'y Cuts at: 1678 2186 5103 1678 Size: 508 2917 2531 Fragments arranged by size: 2917 2531 508 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 3097 3129 4597 Size: 32 135 32 518 32 975 32 1468 Cuts at: 4597 4629 4671 4703 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1468 975 518 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2521 5070 5254 1305 Size: 1216 2549 184 2007 Fragments arranged by size: 2549 2007 1216 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2028 2891 10 Size: 54 1425 343 196 863 3075 Fragments arranged by size: 3075 1425 863 343 196 54 MslI CAynn'nnrTG Cuts at: 518 1166 2270 2462 2993 3914 4273 4432 518 Size: 648 1104 192 531 921 359 159 2042 Fragments arranged by size: 2042 1104 921 648 531 359 192 159 MspA1I CmG'CkG Cuts at: 1606 2171 2290 3002 3130 3372 4049 5197 5442 Size: 565 119 712 128 242 677 1148 245 Cuts at: 5442 1606 Size: 2120 Fragments arranged by size: 2120 1148 712 677 565 245 242 128 119 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4465 Fragments arranged by size: 4465 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 2489 3314 4859 1132 Size: 97 1260 825 1545 2229 Fragments arranged by size: 2229 1545 1260 825 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2185 2368 1108 Size: 1077 183 4696 Fragments arranged by size: 4696 1077 183 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2284 2284 Size: 5956 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 2792 2916 3526 2792 Size: 124 610 5222 Fragments arranged by size: 5222 610 124 PstI C_TGCA'G Cuts at: 830 2640 3308 830 Size: 1810 668 3478 Fragments arranged by size: 3478 1810 668 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 PvuII CAG'CTG Cuts at: 2171 3002 3130 2171 Size: 831 128 4997 Fragments arranged by size: 4997 831 128 SacI G_AGCT'C Cuts at: 2239 2239 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 2516 4204 5916 2516 Size: 1688 1712 2556 Fragments arranged by size: 2556 1712 1688 SexAI A'CCwGG_T Cuts at: 2049 2049 Size: 5956 SfcI C'TryA_G Cuts at: 826 1045 2636 3304 4439 5120 5311 826 Size: 219 1591 668 1135 681 191 1471 Fragments arranged by size: 1591 1471 1135 681 668 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2393 2700 4060 4961 5223 820 Size: 197 926 450 307 1360 901 262 1553 Fragments arranged by size: 1553 1360 926 901 450 307 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 2758 3880 5 Size: 47 2706 1122 2081 Fragments arranged by size: 2706 2081 1122 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5956 StyI C'CwwG_G Cuts at: 513 1065 2004 2023 3395 513 Size: 552 939 19 1372 3074 Fragments arranged by size: 3074 1372 939 552 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3983 4000 4153 4338 4783 325 Size: 1255 2403 17 153 185 445 1498 Fragments arranged by size: 2403 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2514 4202 5914 Size: 274 80 33 51 1443 537 1688 1712 Cuts at: 5914 96 Size: 138 Fragments arranged by size: 1712 1688 1443 537 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5916 5948 5916 Size: 32 5924 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2186 2528 2899 2186 Size: 342 371 5243 Fragments arranged by size: 5243 371 342 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI