################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AgeI Aor51HI ApaI AscI AseI AsnI Asp718I AviII AvrII BbeI BbrPI BcgI BlnI BsaAI BsaBI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrFI BssHII BssSI Bst1107I BstUI BstZ17I Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV FseI FspI HaeII KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcyI 1464 GACGCC G[GA]^CG[CT]C AflII 747 CTTAAG C^TTAAG AhdI 1696 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2786 GTGCAC G^TGCAC AocI 1375 CCTGAGG CC^T[ACGT]AGG ApaLI 2786 GTGCAC G^TGCAC AspEI 1696 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 3394 CCCGAG C^[CT]CG[GA]G BanI 2910 GGCACC G^G[CT][GA]CC BfrI 747 CTTAAG C^TTAAG BglI 3031 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4627 AGATCT A^GATCT BsaHI 1464 GACGCC G[GA]^CG[CT]C BsaWI 1073 ACCGGA [AT]^CCGG[AT] BsoBI 3394 CCCGAG C^[CT]CG[GA]G BspHI 731 TCATGA T^CATGA BsrBI 1589 CCGCTC CCGCTC(-3/-3) BstEII 2310 GGTCACC G^GT[ACGT]ACC BstPI 2310 GGTCACC G^GT[ACGT]ACC Bsu36I 1375 CCTGAGG CC^T[ACGT]AGG DraIII 4703 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3792 GACACGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 4959 CCGTGG C^C[GA][CT]GG Eam1105I 1696 GACAATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1375 CCTGAGG CC^T[ACGT]AGG EcoO65I 2310 GGTCACC G^GT[ACGT]ACC EcoT14I 1968 CCTTGG C^C[AT][AT]GG EcoT22I 1205 ATGCAT ATGCA^T HgaI 1464 GACGC GACGC(5/10) Hin1I 1464 GACGCC G[GA]^CG[CT]C HpaI 546 GTTAAC GTT^AAC MfeI 3767 CAATTG C^AATTG MunI 3767 CAATTG C^AATTG NsiI 1205 ATGCAT ATGCA^T PshAI 3471 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 731 TCATGA T^CATGA SacI 4540 GAGCTC GAGCT^C SalI 4619 GTCGAC G^TCGAC SphI 4202 GCATGC GCATG^C StuI 3689 AGGCCT AGG^CCT StyI 1968 CCTTGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 4619 GTCGAC GT^[AC][GT]AC 4911 GTCTAC AlwNI 1712 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 3489 CAGTCACTG AspI 2523 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2649 GACTCAGTC BalI 2 TGGCCA TGG^CCA 1022 TGGCCA BamHI 18 GGATCC G^GATCC 3681 GGATCC BanII 1785 GGGCTC G[GA]GC[CT]^C 4540 GAGCTC BbsI 1297 GAAGAC GAAGAC(2/6) 1476 GAAGAC BclI 1629 TGATCA T^GATCA 3111 TGATCA BcnI 819 CCGGG CC^[GC]GG 1458 CCGGG BlpI 556 GCTGAGC GC^T[ACGT]AGC 1591 GCTCAGC Bpu1102I 556 GCTGAGC GC^T[ACGT]AGC 1591 GCTCAGC BpuAI 1297 GAAGAC GAAGAC(2/6) 1476 GAAGAC BsaI 1454 GAGACC GGTCTC(1/5) 598 GGTCTC Bsp1407I 1959 TGTACA T^GTACA 3516 TGTACA BspMI 4011 ACCTGC ACCTGC(4/8) 4605 ACCTGC BsrGI 1959 TGTACA T^GTACA 3516 TGTACA BstBI 2096 TTCGAA TT^CGAA 4646 TTCGAA CelII 556 GCTGAGC GC^T[ACGT]AGC 1591 GCTCAGC CfoI 2341 GCGC GCG^C 4127 GCGC FbaI 1629 TGATCA T^GATCA 3111 TGATCA HhaI 2341 GCGC GCG^C 4127 GCGC HinP1I 2341 GCGC G^CGC 4127 GCGC MluNI 2 TGGCCA TGG^CCA 1022 TGGCCA MscI 2 TGGCCA TGG^CCA 1022 TGGCCA NciI 819 CCGGG CC^[GC]GG 1458 CCGGG NdeI 1388 CATATG CA^TATG 5027 CATATG NspV 2096 TTCGAA TT^CGAA 4646 TTCGAA PflFI 2523 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2649 GACTCAGTC SfuI 2096 TTCGAA TT^CGAA 4646 TTCGAA SmlI 747 CTTAAG C^T[CT][GA]AG 3170 CTCAAG SpeI 1084 ACTAGT A^CTAGT 1857 ACTAGT SspBI 1959 TGTACA T^GTACA 3516 TGTACA SspI 256 AATATT AAT^ATT 838 AATATT Tth111I 2523 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2649 GACTCAGTC XcmI 2599 CCAGTTGGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3266 CCACTACTGTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaII BbvCI BbvI BciVI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI