(Linear) (Six-base) MAPSORT of: KIEE3773.seq Check: 6774 from: 1 to: 5229 KIEE3773.orf With 182 enzymes: SgfI * March 22, 2012 16:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 4614 5229 Size: 4614 615 AccI GT'mk_AC Cuts at: 0 4620 4912 5229 Size: 4620 292 317 Fragments arranged by size: 4620 317 292 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 847 1148 1643 1838 2230 2653 2930 3218 Size: 847 301 495 195 392 423 277 288 Cuts at: 3218 3467 4048 4136 4169 5229 Size: 249 581 88 33 1060 Fragments arranged by size: 1060 847 581 495 423 392 301 288 277 249 195 88 33 AflII C'TTAA_G Cuts at: 0 747 5229 Size: 747 4482 AflIII A'CryG_T Cuts at: 0 1635 1962 4671 5229 Size: 1635 327 2709 558 Fragments arranged by size: 2709 1635 558 327 AhdI GACnn_n'nnGTC Cuts at: 0 1701 5229 Size: 1701 3528 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 4746 5219 5229 Size: 4746 473 10 Fragments arranged by size: 4746 473 10 AlwNI CAG_nnn'CTG Cuts at: 0 1717 3494 5229 Size: 1717 1777 1735 Fragments arranged by size: 1777 1735 1717 ApaLI G'TGCA_C Cuts at: 0 2786 5229 Size: 2786 2443 ApoI r'AATT_y Cuts at: 0 56 442 767 1266 1345 1838 2227 2404 Size: 56 386 325 499 79 493 389 177 Cuts at: 2404 2550 4171 4279 4818 5096 5229 Size: 146 1621 108 539 278 133 Fragments arranged by size: 1621 539 499 493 389 386 325 278 177 146 133 108 79 56 AvaI C'yCGr_G Cuts at: 0 3394 5229 Size: 3394 1835 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2995 3028 3611 3644 4130 4163 5229 Size: 2995 33 583 33 486 33 1066 Fragments arranged by size: 2995 1066 583 486 33 33 33 BamHI G'GATC_C Cuts at: 0 18 3681 5229 Size: 18 3663 1548 Fragments arranged by size: 3663 1548 18 BanI G'GyrC_C Cuts at: 0 2910 5229 Size: 2910 2319 BanII G_rGCy'C Cuts at: 0 1789 4544 5229 Size: 1789 2755 685 Fragments arranged by size: 2755 1789 685 BbsI GAAGACnn'nnnn_ Cuts at: 0 1304 1483 5229 Size: 1304 179 3746 Fragments arranged by size: 3746 1304 179 BbvCI CC'TCA_GC Cuts at: 0 1217 1556 3887 5229 Size: 1217 339 2331 1342 Fragments arranged by size: 2331 1342 1217 339 BciVI GTATCCnnnnn_n' Cuts at: 0 1046 1215 2052 3023 3402 5087 5229 Size: 1046 169 837 971 379 1685 142 Fragments arranged by size: 1685 1046 971 837 379 169 142 BclI T'GATC_A Cuts at: 0 1629 3111 5229 Size: 1629 1482 2118 Fragments arranged by size: 2118 1629 1482 BfrBI ATG'CAT Cuts at: 0 1207 5229 Size: 1207 4022 BglI GCCn_nnn'nGGC Cuts at: 0 3037 5229 Size: 3037 2192 BglII A'GATC_T Cuts at: 0 4627 5229 Size: 4627 602 BlpI GC'TnA_GC Cuts at: 0 557 1592 5229 Size: 557 1035 3637 Fragments arranged by size: 3637 1035 557 Bme1580I G_kGCm'C Cuts at: 0 2790 5229 Size: 2790 2439 BmgBI CAC'GTC Cuts at: 0 4618 5229 Size: 4618 611 BmrI ACTGGGnnnn_n' Cuts at: 0 1610 1748 2593 4653 5229 Size: 1610 138 845 2060 576 Fragments arranged by size: 2060 1610 845 576 138 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3675 5229 Size: 3675 1554 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 586 935 2282 2308 2504 3328 4727 5229 Size: 586 349 1347 26 196 824 1399 502 Fragments arranged by size: 1399 1347 824 586 502 349 196 26 Bpu10I CC'TnA_GC Cuts at: 0 1217 1556 3887 5229 Size: 1217 339 2331 1342 Fragments arranged by size: 2331 1342 1217 339 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3155 5229 Size: 3155 2074 BsaI GGTCTCn'nnnn_ Cuts at: 0 604 1448 5229 Size: 604 844 3781 Fragments arranged by size: 3781 844 604 BsaHI Gr'CG_yC Cuts at: 0 1465 5229 Size: 1465 3764 BsaWI w'CCGG_w Cuts at: 0 1073 5229 Size: 1073 4156 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 786 816 2640 2670 4982 5012 5199 5229 Size: 786 30 1824 30 2312 30 187 30 Fragments arranged by size: 2312 1824 786 187 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 479 794 1671 2576 2648 5229 Size: 479 315 877 905 72 2581 Fragments arranged by size: 2581 905 877 479 315 72 BseYI C'CCAG_C Cuts at: 0 3631 4252 4517 5229 Size: 3631 621 265 712 Fragments arranged by size: 3631 712 621 265 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 780 2975 5215 5229 Size: 780 2195 2240 14 Fragments arranged by size: 2240 2195 780 14 BsiHKAI G_wGCw'C Cuts at: 0 2790 3374 4544 5229 Size: 2790 584 1170 685 Fragments arranged by size: 2790 1170 685 584 BsmI GAATG_Cn' Cuts at: 0 1062 1896 2673 5039 5229 Size: 1062 834 777 2366 190 Fragments arranged by size: 2366 1062 834 777 190 Bsp1286I G_dGCh'C Cuts at: 0 1789 2790 3374 4544 5229 Size: 1789 1001 584 1170 685 Fragments arranged by size: 1789 1170 1001 685 584 BspHI T'CATG_A Cuts at: 0 731 5229 Size: 731 4498 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 4020 4614 5229 Size: 4020 594 615 Fragments arranged by size: 4020 615 594 BsrBI CCG'CTC Cuts at: 0 1591 5229 Size: 1591 3638 BsrDI GCAATG_nn' Cuts at: 0 257 2580 2752 5229 Size: 257 2323 172 2477 Fragments arranged by size: 2477 2323 257 172 BsrGI T'GTAC_A Cuts at: 0 1959 3516 5229 Size: 1959 1557 1713 Fragments arranged by size: 1959 1713 1557 BstAPI GCAn_nnn'nTGC Cuts at: 0 789 1213 1717 1923 5229 Size: 789 424 504 206 3306 Fragments arranged by size: 3306 789 504 424 206 BstBI TT'CG_AA Cuts at: 0 2097 4647 5229 Size: 2097 2550 582 Fragments arranged by size: 2550 2097 582 BstEII G'GTnAC_C Cuts at: 0 2310 5229 Size: 2310 2919 BstXI CCAn_nnnn'nTGG Cuts at: 0 2053 4960 5087 5229 Size: 2053 2907 127 142 Fragments arranged by size: 2907 2053 142 127 BstYI r'GATC_y Cuts at: 0 18 822 862 2447 3681 4213 4627 4698 Size: 18 804 40 1585 1234 532 414 71 Cuts at: 4698 5229 Size: 531 Fragments arranged by size: 1585 1234 804 532 531 414 71 40 18 Bsu36I CC'TnA_GG Cuts at: 0 1376 5229 Size: 1376 3853 BtgI C'CryG_G Cuts at: 0 4959 5229 Size: 4959 270 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 3853 5229 Size: 3853 1376 BtsI GCAGTG_nn' Cuts at: 0 1674 4308 4428 5229 Size: 1674 2634 120 801 Fragments arranged by size: 2634 1674 801 120 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2469 2504 5229 Size: 2469 35 2725 Fragments arranged by size: 2725 2469 35 DraI TTT'AAA Cuts at: 0 474 726 773 5229 Size: 474 252 47 4456 Fragments arranged by size: 4456 474 252 47 DraIII CAC_nnn'GTG Cuts at: 0 4708 5229 Size: 4708 521 DrdI GACnn_nn'nnGTC Cuts at: 0 3798 5229 Size: 3798 1431 EaeI y'GGCC_r Cuts at: 0 2 1022 2694 5229 Size: 2 1020 1672 2535 Fragments arranged by size: 2535 1672 1020 2 EarI CTCTTCn'nnn_ Cuts at: 0 160 456 2081 2187 2672 3746 4067 4399 Size: 160 296 1625 106 485 1074 321 332 Cuts at: 4399 5229 Size: 830 Fragments arranged by size: 1625 1074 830 485 332 321 296 160 106 EcoICRI GAG'CTC Cuts at: 0 4542 5229 Size: 4542 687 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 586 847 935 1148 1643 1838 2230 2282 Size: 586 261 88 213 495 195 392 52 Cuts at: 2282 2308 2504 2653 2930 3218 3328 3467 4048 Size: 26 196 149 277 288 110 139 581 Cuts at: 4048 4136 4169 4727 5229 Size: 88 33 558 502 Fragments arranged by size: 586 581 558 502 495 392 288 277 261 213 196 195 149 139 110 88 88 52 33 26 EcoO109I rG'GnC_Cy Cuts at: 0 698 853 1191 2022 3606 5229 Size: 698 155 338 831 1584 1623 Fragments arranged by size: 1623 1584 831 698 338 155 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 729 2092 5229 Size: 729 1363 3137 Fragments arranged by size: 3137 1363 729 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 713 745 864 896 1246 1278 1687 1719 Size: 713 32 119 32 350 32 409 32 Cuts at: 1719 1773 1805 2606 2638 2640 2670 2672 3442 Size: 54 32 801 32 2 30 2 770 Cuts at: 3442 3474 3745 3777 4417 4449 4929 4961 4982 Size: 32 271 32 640 32 480 32 21 Cuts at: 4982 5014 5229 Size: 32 215 Fragments arranged by size: 801 770 713 640 480 409 350 271 215 119 54 32 32 32 32 32 32 32 32 32 32 32 30 21 2 2 HincII GTy'rAC Cuts at: 0 335 548 4621 4861 5229 Size: 335 213 4073 240 368 Fragments arranged by size: 4073 368 335 240 213 HindIII A'AGCT_T Cuts at: 0 274 2100 3550 4367 5229 Size: 274 1826 1450 817 862 Fragments arranged by size: 1826 1450 862 817 274 HpaI GTT'AAC Cuts at: 0 548 5229 Size: 548 4681 MfeI C'AATT_G Cuts at: 0 3767 5229 Size: 3767 1462 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2034 2534 3558 5206 5229 Size: 2034 500 1024 1648 23 Fragments arranged by size: 2034 1648 1024 500 23 MscI TGG'CCA Cuts at: 0 4 1024 5229 Size: 4 1020 4205 Fragments arranged by size: 4205 1020 4 MslI CAynn'nnrTG Cuts at: 0 898 974 1421 2816 3007 3934 3959 4932 Size: 898 76 447 1395 191 927 25 973 Cuts at: 4932 5229 Size: 297 Fragments arranged by size: 1395 973 927 898 447 297 191 76 25 MspA1I CmG'CkG Cuts at: 0 2286 2965 3808 3858 5229 Size: 2286 679 843 50 1371 Fragments arranged by size: 2286 1371 843 679 50 NdeI CA'TA_TG Cuts at: 0 1389 5028 5229 Size: 1389 3639 201 Fragments arranged by size: 3639 1389 201 NsiI A_TGCA'T Cuts at: 0 1209 5229 Size: 1209 4020 NspI r_CATG'y Cuts at: 0 1639 1966 4206 4675 5229 Size: 1639 327 2240 469 554 Fragments arranged by size: 2240 1639 554 469 327 PciI A'CATG_T Cuts at: 0 1635 1962 4671 5229 Size: 1635 327 2709 558 Fragments arranged by size: 2709 1635 558 327 PfoI T'CCnGG_A Cuts at: 0 3275 5229 Size: 3275 1954 PpuMI rG'GwC_Cy Cuts at: 0 698 853 1191 2022 3606 5229 Size: 698 155 338 831 1584 1623 Fragments arranged by size: 1623 1584 831 698 338 155 PshAI GACnn'nnGTC Cuts at: 0 3475 5229 Size: 3475 1754 PstI C_TGCA'G Cuts at: 0 536 3734 4538 5229 Size: 536 3198 804 691 Fragments arranged by size: 3198 804 691 536 PvuII CAG'CTG Cuts at: 0 2286 2965 3808 5229 Size: 2286 679 843 1421 Fragments arranged by size: 2286 1421 843 679 SacI G_AGCT'C Cuts at: 0 4544 5229 Size: 4544 685 SalI G'TCGA_C Cuts at: 0 4619 5229 Size: 4619 610 SanDI GG'GwC_CC Cuts at: 0 853 5229 Size: 853 4376 SapI GCTCTTCn'nnn_ Cuts at: 0 2081 2187 4067 5229 Size: 2081 106 1880 1162 Fragments arranged by size: 2081 1880 1162 106 SfcI C'TryA_G Cuts at: 0 155 410 532 2762 3185 3730 4534 4694 Size: 155 255 122 2230 423 545 804 160 Cuts at: 4694 5120 5229 Size: 426 109 Fragments arranged by size: 2230 804 545 426 423 255 160 155 122 109 SmlI C'TyrA_G Cuts at: 0 747 3170 5229 Size: 747 2423 2059 Fragments arranged by size: 2423 2059 747 SpeI A'CTAG_T Cuts at: 0 1084 1857 5229 Size: 1084 773 3372 Fragments arranged by size: 3372 1084 773 SphI G_CATG'C Cuts at: 0 4206 5229 Size: 4206 1023 SspI AAT'ATT Cuts at: 0 258 840 5229 Size: 258 582 4389 Fragments arranged by size: 4389 582 258 StuI AGG'CCT Cuts at: 0 3691 5229 Size: 3691 1538 StyI C'CwwG_G Cuts at: 0 1968 5229 Size: 1968 3261 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 554 1989 4547 5229 Size: 554 1435 2558 682 Fragments arranged by size: 2558 1435 682 554 TatI w'GTAC_w Cuts at: 0 1959 2541 3135 3516 5082 5229 Size: 1959 582 594 381 1566 147 Fragments arranged by size: 1959 1566 594 582 381 147 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 786 814 818 846 1220 1252 1513 1545 Size: 786 28 4 28 374 32 261 32 Cuts at: 1545 4008 4040 4298 4330 5199 5229 Size: 2463 32 258 32 869 30 Fragments arranged by size: 2463 869 786 374 261 258 32 32 32 32 30 28 28 4 Tth111I GACn'n_nGTC Cuts at: 0 2526 2652 5229 Size: 2526 126 2577 Fragments arranged by size: 2577 2526 126 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2606 3273 5229 Size: 2606 667 1956 Fragments arranged by size: 2606 1956 667 XmnI GAAnn'nnTTC Cuts at: 0 1549 2196 4553 5229 Size: 1549 647 2357 676 Fragments arranged by size: 2357 1549 676 647 Enzymes that do cut: AarI AccI AcuI AflII AflIII AhdI AlfI AlwNI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NdeI NsiI NspI PciI PfoI PpuMI PshAI PstI PvuII SacI SalI SanDI SapI SfcI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AgeI AleI AloI ApaI AscI AseI AsiSI AvrII BbeI BcgI BmtI BsaAI BsaBI BsiEI BsiWI BsmBI BspEI BsrFI BssHII BssSI BstZ17I ClaI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI XbaI XhoI XmaI ZraI