(Circular) (Six-base) MAPSORT of: pFN21AB4983.seq Check: 2849 from: 1 to: 6232 pFN21AB4983 6232 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3575 1937 Size: 1638 4594 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2928 3216 4295 5679 5947 1632 Size: 1296 288 1079 1384 268 1917 Fragments arranged by size: 1917 1384 1296 1079 288 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6035 AflIII A'CryG_T Cuts at: 1203 5131 6108 1203 Size: 3928 977 1327 Fragments arranged by size: 3928 1327 977 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 4961 4961 Size: 6232 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6232 AlwNI CAG_nnn'CTG Cuts at: 3383 3975 5547 3383 Size: 592 1572 4068 Fragments arranged by size: 4068 1572 592 ApaLI G'TGCA_C Cuts at: 3149 4289 5445 3149 Size: 1140 1156 3936 Fragments arranged by size: 3936 1156 1140 ApoI r'AATT_y Cuts at: 1493 2359 2732 2816 2965 3012 3042 3263 3541 Size: 866 373 84 149 47 30 221 278 Cuts at: 3541 3767 3919 6028 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 866 373 278 226 221 152 149 84 47 30 AseI AT'TA_AT Cuts at: 160 1032 4786 160 Size: 872 3754 1606 Fragments arranged by size: 3754 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 3557 1870 Size: 73 1614 4545 Fragments arranged by size: 4545 1614 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2589 2622 2589 Size: 33 6199 BamHI G'GATC_C Cuts at: 3562 3929 3562 Size: 367 5865 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3553 5008 618 Size: 325 217 627 1766 1455 1842 Fragments arranged by size: 1842 1766 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3551 1391 Size: 2160 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5261 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4423 4457 1903 Size: 34 50 34 2402 34 3678 Fragments arranged by size: 3678 2402 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4124 5334 4124 Size: 1210 5022 BclI T'GATC_A Cuts at: 1548 1548 Size: 6232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4843 136 Size: 107 122 71 631 605 3171 1525 Fragments arranged by size: 3171 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6227 6227 Size: 6232 BlpI GC'TnA_GC Cuts at: 3649 3649 Size: 6232 Bme1580I G_kGCm'C Cuts at: 1790 3153 4293 5449 1790 Size: 1363 1140 1156 2573 Fragments arranged by size: 2573 1363 1156 1140 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2182 3365 3963 Size: 416 53 83 399 24 765 1183 598 Cuts at: 3963 4921 442 Size: 958 1753 Fragments arranged by size: 1753 1183 958 765 598 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2035 2178 3405 4892 1239 Size: 796 143 1227 1487 2579 Fragments arranged by size: 2579 1487 1227 796 143 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4357 5222 5520 4357 Size: 865 298 5069 Fragments arranged by size: 5069 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4895 882 Size: 617 3396 2219 Fragments arranged by size: 3396 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4023 493 Size: 711 2819 2702 Fragments arranged by size: 2819 2702 711 BsaBI GATnn'nnATC Cuts at: 6226 6226 Size: 6232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4039 4421 5056 275 Size: 53 83 186 936 2506 382 635 1451 Fragments arranged by size: 2506 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4665 5063 5337 5484 5895 4665 Size: 398 274 147 411 5002 Fragments arranged by size: 5002 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2151 2181 3378 3408 1178 Size: 30 165 30 748 30 1197 30 4002 Fragments arranged by size: 4002 1197 748 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6232 BseYI C'CCAG_C Cuts at: 2983 5435 5931 2983 Size: 2452 496 3284 Fragments arranged by size: 3284 2452 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2685 2733 1163 Size: 573 949 48 4662 Fragments arranged by size: 4662 949 573 48 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3682 4443 5471 1540 Size: 275 186 1681 761 1028 2301 Fragments arranged by size: 2301 1681 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3153 3364 4293 4378 5449 3153 Size: 211 929 85 1071 3936 Fragments arranged by size: 3936 1071 929 211 85 BsmI GAATG_Cn' Cuts at: 3743 3836 3743 Size: 93 6139 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3153 3364 3551 4293 4378 5449 1391 Size: 399 1363 211 187 742 85 1071 2174 Fragments arranged by size: 2174 1363 1071 742 399 211 187 85 BspEI T'CCGG_A Cuts at: 5895 5895 Size: 6232 BspHI T'CATG_A Cuts at: 4121 5851 4121 Size: 1730 4502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3587 844 Size: 2743 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4119 1394 Size: 429 2296 3507 Fragments arranged by size: 3507 2296 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4727 4901 65 Size: 1183 3479 174 1396 Fragments arranged by size: 3479 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4876 5063 1700 Size: 110 3066 187 2869 Fragments arranged by size: 3066 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6232 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 4292 5304 4292 Size: 1012 5220 BstAPI GCAn_nnn'nTGC Cuts at: 794 3500 794 Size: 2706 3526 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6232 BstYI r'GATC_y Cuts at: 1972 2998 3324 3562 3929 4314 4331 5783 5869 Size: 1026 326 238 367 385 17 1452 86 Cuts at: 5869 5881 6227 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1026 385 367 346 326 238 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2308 2550 513 Size: 552 1243 242 4195 Fragments arranged by size: 4195 1243 552 242 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5493 Fragments arranged by size: 5493 683 56 BtsI GCAGTG_nn' Cuts at: 2097 2490 3748 4542 4562 2097 Size: 393 1258 794 20 3767 Fragments arranged by size: 3767 1258 794 393 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6197 DraI TTT'AAA Cuts at: 2632 2815 2821 3536 2632 Size: 183 6 715 5328 Fragments arranged by size: 5328 715 183 6 DraIII CAC_nnn'GTG Cuts at: 2234 2234 Size: 6232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5239 809 Size: 507 3923 1802 Fragments arranged by size: 3923 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2944 3679 Size: 54 1425 50 275 18 207 907 735 Cuts at: 3679 4568 6197 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 907 889 735 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2378 2973 3216 3231 4162 1353 Size: 1025 595 243 15 931 3423 Fragments arranged by size: 3423 1025 931 595 243 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4819 5193 5339 387 Size: 1064 138 3230 374 146 1280 Fragments arranged by size: 3230 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2035 2178 2928 3216 3405 4295 4892 Size: 393 403 143 750 288 189 890 597 Cuts at: 4892 5679 5947 1239 Size: 787 268 1524 Fragments arranged by size: 1524 890 787 750 597 403 393 288 268 189 143 EcoRI G'AATT_C Cuts at: 3012 3541 3919 3012 Size: 529 378 5325 Fragments arranged by size: 5325 529 378 EcoRV GAT'ATC Cuts at: 2775 2775 Size: 6232 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3425 3515 3425 Size: 90 6142 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 5379 1678 Size: 3701 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 2309 2341 4873 Size: 32 135 32 430 32 275 32 2532 Cuts at: 4873 4905 4947 4979 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2532 430 275 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3576 3822 669 Size: 988 281 1638 246 3079 Fragments arranged by size: 3079 1638 988 281 246 HindIII A'AGCT_T Cuts at: 748 2400 3480 748 Size: 1652 1080 3500 Fragments arranged by size: 3500 1652 1080 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 3163 3831 3163 Size: 668 5564 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2737 3491 5346 5530 1305 Size: 1432 754 1855 184 2007 Fragments arranged by size: 2007 1855 1432 754 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2039 10 Size: 54 1425 343 207 4203 Fragments arranged by size: 4203 1425 343 207 54 MslI CAynn'nnrTG Cuts at: 518 1166 2454 2748 4190 4549 4708 518 Size: 648 1288 294 1442 359 159 2042 Fragments arranged by size: 2042 1442 1288 648 359 294 159 MspA1I CmG'CkG Cuts at: 1606 3648 4325 5473 5718 1606 Size: 2042 677 1148 245 2120 Fragments arranged by size: 2120 2042 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2308 513 Size: 552 1243 4437 Fragments arranged by size: 4437 1243 552 NdeI CA'TA_TG Cuts at: 387 2301 387 Size: 1914 4318 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6232 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 2244 3590 5135 1132 Size: 97 1015 1346 1545 2229 Fragments arranged by size: 2229 1545 1346 1015 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6232 PciI A'CATG_T Cuts at: 5131 5131 Size: 6232 PflMI CCAn_nnn'nTGG Cuts at: 1108 3383 1108 Size: 2275 3957 PfoI T'CCnGG_A Cuts at: 2155 2575 2155 Size: 420 5812 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4301 4333 5814 5846 4301 Size: 32 1481 32 4687 Fragments arranged by size: 4687 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6232 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PstI C_TGCA'G Cuts at: 830 2092 2395 3584 830 Size: 1262 303 1189 3478 Fragments arranged by size: 3478 1262 1189 303 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6232 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SapI GCTCTTCn'nnn_ Cuts at: 2973 3216 2973 Size: 243 5989 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 2389 3354 4480 6192 2389 Size: 965 1126 1712 2429 Fragments arranged by size: 2429 1712 1126 965 SexAI A'CCwGG_T Cuts at: 3376 3376 Size: 6232 SfcI C'TryA_G Cuts at: 826 1045 2075 2088 2391 2613 3580 4715 5396 Size: 219 1030 13 303 222 967 1135 681 Cuts at: 5396 5587 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1030 967 681 303 222 219 191 13 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 3559 1872 Size: 1687 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4336 5237 5499 820 Size: 197 926 2393 901 262 1553 Fragments arranged by size: 2393 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3590 1132 Size: 2458 3774 SspI AAT'ATT Cuts at: 5 52 4156 5 Size: 47 4104 2081 Fragments arranged by size: 4104 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6232 StyI C'CwwG_G Cuts at: 513 1065 2034 2308 3328 3671 513 Size: 552 969 274 1020 343 3074 Fragments arranged by size: 3074 1020 969 552 343 274 SwaI ATTT'AAAT Cuts at: 2821 2821 Size: 6232 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4259 4276 4429 4614 5059 325 Size: 1255 2679 17 153 185 445 1498 Fragments arranged by size: 2679 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2025 2387 2926 Size: 274 80 33 51 1443 48 362 539 Cuts at: 2926 3249 3352 4478 6190 96 Size: 323 103 1126 1712 138 Fragments arranged by size: 1712 1443 1126 539 362 323 274 138 103 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6192 6224 6192 Size: 32 6200 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6232 XbaI T'CTAG_A Cuts at: 2327 3568 2327 Size: 1241 4991 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2306 2306 Size: 6232 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6232 XmaI C'CCGG_G Cuts at: 1870 3557 1870 Size: 1687 4545 XmnI GAAnn'nnTTC Cuts at: 4361 4361 Size: 6232 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI